[Comparative characterization of walking biomechanical parameters in hip prosthesis users with mechanical and electronic knee modules]. / Sravnitel'naya kharakteristika biomekhanicheskikh parametrov khod'by u pol'zovatelei protezov bedra s mekhanicheskim i elektronnym kolennymi modulyami.

According to many studies, prostheses with electronic knee modules have some important technical advantages over mechanical knee modules, allowing the user to have a more active lifestyle and significantly improving the overall quality of life. However, we have found no published Russian studies of the biomechanics of walking on such prostheses for more than 20 years of their use in Russia. OBJECTIVE: To compare spatial and temporal parameters of walking in patients after hip replacement using prostheses with electronic and mechanical knee modules. MATERIALS AND METHODS: The study included 94 patients aged between 18 and 50 with hip prostheses. The patients were divided into two groups: Group 1 included 48 patients with a hip prosthesis with a mechanical knee module; Group 2 included 46 patients with a hip prosthesis with an electronic knee module. The groups were comparable in size, gender and age distribution, and mobility grade (grade 3 according to the MOBIS classification system). All patients had no comorbidities or injuries that could limit mobility. Spatial and temporal parameters of walking on hip prostheses with different knee modules were assessed using a video analysis system (SMART DX5000, Italy). RESULTS: Group 2 patients (prostheses with electronic knee modules) had the following spatial and temporal biomechanical walking indices: arbitrary walking speed 1.10-1.08 m/s, walking pace 105.82 steps/minute, step cycle 1.13-1.15 s, double step length 1.27 m, rhythm index 0.89. Group 1 patients (prostheses with mechanical knee modules) had worse outcomes according to the same indices: walking speed 0.81-0.87 m/s, walking pace 90.84 steps/minute, step cycle 1.33 s, double step length 1.07 m, rhythm index 0.74. Preferred walking speed and walking pace indices in patients with electronic knee modules in hip prostheses were at the lower limits of reference values in healthy individuals; the step length was insignificantly reduced; the rhythm index was close to that of healthy individuals (0.94-0.97). CONCLUSION: The results of the study show that the use of prostheses with electronic knee modules in patients with amputation at the hip level enables patients to learn better symmetrical walking and significantly increases their mobility compared to mechanical modules.

[Cytokine profile changes in children with juvenile idiopathic arthritis-associated uveitis]. / Izmenenie tsitokinovogo profilya u detei s uveitom, assotsiirovannym s yuvenil'nym idiopaticheskim artritom.

AIM: to identify the differences between serum cytokine profiles in juvenile idiopathic arthritis (JIA) with or without uveal tract inflammation. MATERIAL AND METHODS: Serum cytokine profiles were studied in two groups of patients: 20 children with JIA and JIA-associated uveitis and 33 children, who had no signs of uveitis under basic therapy for their JIA. All the patients showed drug remission of articular syndrome. Inflammation of the choroid took the form of chronic anterior uveitis. The process was active in 95% of cases. The control group consisted of 35 children without rheumatic disease or other acute condition at the time of examination. Groups were comparable in terms of age and sex. Serum levels of TNF-α, IFN-γ, IL-17, IL-10 were measured by the enzyme multiplied immunoassay technique. RESULTS: A statistically significant increase in TNF-α, IFN-γ, IL-17, and IL-10 levels was found in all patients as compared to the control group. A comparison drawn between serum cytokine levels of JIA patients and those, who also suffered from JIA-associated uveitis, revealed a decrease in IFN-γ and an increase in IL-10 in the latter group. There was also a statistically significant positive correlation between TNF-α and IFN-γ serum levels in patients with JIA-associated uveitis. CONCLUSION: Development of uveitis in patients with drug remission of JIA occurs on the background of cytokine imbalance in the serum, in particular, increased concentrations of proinflammatory TNF-α and IL-17 cytokines along with reduced IFN-γ and increased IL-10 levels. This may be regarded as risk factors for ocular inflammation and should be taken into account when making treatment decisions.

[FEATURES OF MODIFYING EFFECT OF LOW-INTENSITY ELECTROMAGNETIC RADIATION OF NATURAL AND TECHNOGENIC ORIGIN ON VIABILITY AND FUNCTIONAL STATUS OF NEUTROPHILIC GRANULOCYTES].

AIM: Study effects of microwave electromagnetic radiation (EMR) of the SHF range in vitro on viability and functional status of neutrophilic granulocytes. MATERIALS AND METHODS: Neutrophils of peripheral blood of 30 conditionally healthy donors aged 18 - 20 years were exposed to the effect of broadband (natural, similar to sun radio emission) and, mono-frequency (technogenic, similar to emission of cell phones, PCs, microwave ovens, etc.) EMR with frequency range of 4 - 4.34 GHz, generated by a SHF-generator <>. 16 minutes after the effect, viability of neutrophilic granulocytes, phagocytic activity, lysosome activity ofcells were studied, NBT-reducing ability and cytokine content in supernatant were evaluated. RESULTS: The amount of viable neu- trophils significantly reduced after the effect of modelled EMR with technogenic frequency- temporal structure, functional status of viable cells did not change. Neutrophils retained viability after the effect of EMR of natural origin, increased levels of IL1ß and TNFα was determined in supernatants, functional status of neutrophils of men remained stable, and in women an increase of phagocytic ability and a reduction of production of oxygen radicals was noted. CONCLUSION: The detected reduction of the amount of viable neutrophils under the effect of EMR of technogenic origin could result in disturbances in the system of innate immunity, other homeostasis elements of the human organism and development of pathologic conditions. At the same time, the de- tected effects of EMR of natural origin. open perspectives of use of modelled microwave EMR of SHF range in prophylaxis and clinical medicine.

[Cytokine status of cervical mucus in women with transitory and persistent course of papillomavirus infection].

AIM: Study the levels of cytokines in cervical mucus of women with transitory and persistent course of papillomavirus infection (PVI). MATERIALS AND METHODS: Material from 122 women was studied: 32 patients in control group, 68 patients with transitory course of PVI and 52 women with persistent course of PVI. The presence or absence of high risk HPV DNA was confirmed by real time PCR. High risk HPV DNA was determined in scrapes of epithelial cells of cervical canal. Quantitative determination of cytokines (IFN-alpha, IFN-gamma, IL-1beta, receptor antagonist IL-1, IL-2, IL-4, IL-8 and IL-10, TNFalpha in cervical mucus was performed by enzyme immunoassay. RESULTS: Transitory course of PVI was characterized by an increase of IFN-alpha and IFN-gamma, persistent course of PIV--by a decrease of IFN-alpha, IL-2 level was increased in both groups of patients with PVI. CONCLUSION: The outcome of PVI is controlled by interferon component of the immunity. Because of this during observation of patients with PVI the determination of IFN-alpha, IFN-gamma content in cervical mucus is an appropriate diagnostic procedure in clinical practice for the prognosis of risk of development of high risk PIV persistency.

[Influence of Solcotrichovac vaccine on formation of neutrophil extracellular traps].

AIM: To study the influence of Solcotichovac vaccine on formation of neutrophil extracellular traps. MATERIALS AND METHODS: Solcotrichovac vaccine administered in various doses was used in order to assess the formation of neutrophil extracellular traps in peripheral blood of women of childbearing age. RESULTS: Therapeutic dose of Solcotrichovac stimulated the most prominent formation of neutrophil extracellular traps. CONCLUSION: Solcotrichovac vaccine stimulated non-specific arm of mucosal immunity due to formation of neutrophil extracellular traps and it is probable that this mechanism explains the effect of this vaccine.

[Effects of sodium hydrogen carbonate-chloride-containing mineral water on stress sensitivity in rats]. / Otsenka vliianiia gidrokarbonatno-khloridnoi natrievoi mineral'noi vody na chuvstvitel'nost' krys k deistviiu stressa.

The investigation used the mineral water (MW) Uralochka obtained from well 4638 located on the territory of the sanatorium Ural (the village of Khomutino, Chelyabinsk Region). Experimental findings suggest that the use of the Uralochka water for 14 days lowers experimental animals' sensitivity to stress. This is manifested by that MW use virtually completely eliminated the hematological manifestations of stress, poststress depression of a cell immunity response and prevented the reduction of cell counts in the abdominal cavity. The immunostimulating effect of the Uralochka MW on humoral immunity and the phagocytosis of peritoneal macrophages preserved with the combined influence of stress and MW.

[An evaluation of the effect of sodium bicarbonate-chloride mineral water on the rat immune system]. / Otsenka vliianiia gidrokarbonatno-khloridnoi natrievoi mineral'noi vody na immunnuiu sistemu krys.

Mineral water Uralochka tried in rats is obtained from the spring in the territory of the sanatorium Ural (Chelyabinsk Province). The mineral water contains chloride, hydrocarbonate ions and sodium cations, mineralization is 4.13 g/dm3. A 15-day course of Uralochka drinking did not influence much reaction of delayed hypersensitivity, but increased the absolute number of antibody-forming cells by 95.86%, raised the ability of peritoneal macrophages to absorb latex particles. Thus, mineral water Uralochka can raise intensity of humoral immune response and the ability of macrophages for phagocytosis of the latex particles. Phagocytosis activity and intensity increased by 48.27 and 62.13%, respectively.