Probing the dynamics of three freshwater Anammox genera at different salinity levels in a partial nitritation and Anammox sequencing batch reactor treating landfill leachate.

Partial nitritation/Anammox was applied to treat NaCl-amended landfill leachate. The reactor established robust nitrogen removal of 85.7 ± 2.4% with incremental salinity from 0.61% to 3.10% and achieved 0.91-1.05 kg N/m3/d at salinity of 2.96%-3.10%. Microbial community analysis revealed Nitrosomonas, Nitrospira, and denitrifiers occupied 4.1%, <0.2% and 10.9%, respectively. Salinity variations impelled the dynamics of Anammox bacteria. Jettenia shifted to Brocadia and Kuenenia at salinity of 0.61%-0.81%. Kuenenia outcompeted Brocadia and occupied 51.5% and 50.9% at salinity of 1.48%-1.54% and 2.96%-3.10%, respectively. High nitrite affinity and fast growth rate were proposed as key factors fostering Brocadia overgrew Jettenia. Functionalities of sodium-motive-force facilitated energy generation and intracellular osmotic pressure equilibrium regulation crucially determined Kuenenia's dominance at elevated salinity. Co-occurrence network further manifested beneficial symbiotic relationships boosted Kuenenia's preponderance. Knowledge gleaned deepen understanding on survival niches of freshwater Anammox genera at saline environments and lead to immediate benefits to its applications treating relevant wastewaters.

Unclassified Anammox bacterium responds to robust nitrogen removal in a sequencing batch reactor fed with landfill leachate.

Treatment of landfill leachate was conducted in a lab-scale sequencing batch reactor (SBR). The SBR was started through inoculating activated sludge with controlling dissolved oxygen of 0.5-1.0 mg/L. Anammox reaction took place within around three months. The SBR established robust nitrogen removal with incremental NLRs of 0.25-2.17 kg N/m3/d. At the final phase, it achieved elevated nitrogen removals of 1.68-1.91 kg N/m3/d. 16S rRNA gene amplicon sequencing analysis revealed Nitrosomonas, unclassified Anammox bacterium, and diverse denitrifying populations coexisted and accounted for 4.02%, 20.05% and 34.69%, respectively. Phylogenic analysis and average nucleotide identity comparison jointly suggested the unclassified Anammox bacterium potentially pertained to a novel Anammox lineage. The functional profiles' prediction suggested sulfate reduction, arsenate reduction and eliminations of antibiotics and drugs likely occurred in the SBR. The finding from this study suggests contribution of unclassified Anammox bacteria in influencing nitrogen budget in natural and engineering systems is currently being underestimated.

Efficient transition from partial nitritation to partial nitritation/Anammox in a membrane bioreactor with activated sludge as the sole seed source.

A lab-scale membrane bioreactor (MBR) was employed to carry out the partial nitritation/Anammox (PN/A) process from conventional activated sludge. Seed sludge was cultivated under microaerobic conditions for 10 days before seeding into the MBR. The bacterial community was analyzed on the basis of cloning and sequencing of 16S rRNA gene. Relative slow ammonia oxidation rates (3.2-13.0 mgN/L/d) were established in the microaerobic cultivation period. In the continuous MBR operation, the nitritation was achieved in the first 16 days and the reactor produced a balanced ratio between ammonia and nitrite which favored the proliferation of Anammox bacteria. Efficient transition from PN to PN/A was achieved in two months which was supported by appearance of reddish spots on the reactor inner wall and the concurrent consumption of ammonium and nitrite. The PN/A performed a robust and high-rate nitrogen removal capability and achieved a peak nitrogen removal of 1.81 kg N/m3/d. 16S rRNA gene-based analysis indicated that "Nitrosomonas sp." and "Candidatus Jettenia sp." accounted for ammonia oxidation and nitrogen depletion, respectively. Denitratisoma facilitated denitrification in the reactor. The present study suggested that a pre-cultivation of seed sludge under microaerobic conditions assists fast realization of PN and further convoyed efficient transition from PN to PN/A. Knowledge gleaned from this study is of significance to initiation, operation, and control of MBR-PN/As.