RESUMEN
Polysiloxanes have shown exquisite properties for fabrication of microstructures for various biomedical and biotechnological applications. Nevertheless, their biocompatibility in terms of cell adhesion and survival ability is controversial. A simple polysiloxane modifying procedure that reproducibly enhances cell adhesion was proposed. Sonication of the hybrid organic-inorganic polymer of polysiloxane type, Ormocomp, in potassium hydroxide (KOH)/ethanol solution enhanced adhesion and subsequent survival of a panel of four cell lines. Characterization of surface properties of untreated and KOH-treated Ormocomp coatings has revealed considerable negative charge of Ormocomp substrates based on measurements of zeta potentials. KOH treatment did not modify surface morphology as visualized by scanning electron microscopy, but it resulted in alteration in both chemical composition according to SIMS analysis and hydrophilicity evaluated by static water contact angles. The results suggest that the failure of the adherent cells to survive on Ormocomp coatings is related to cell adhesion. The negative surface charge of Ormocomp substrates may be one of the influencing factors; however, the modification of surface chemistry mediated by KOH and the resulting increase in hydrophilicity accompanied by modification of protein adsorption are more likely responsible for enhanced cell adhesion and survival on Ormocomp coatings. KOH treatment thus may serve as a simple, cost-effective procedure modifying polysiloxane-type polymers that leads to reproducible enhancement of cell adhesion.
Asunto(s)
Materiales Biocompatibles/metabolismo , Adhesión Celular , Hidróxidos/metabolismo , Compuestos de Potasio/metabolismo , Siloxanos/metabolismo , Rayos Ultravioleta , Animales , Materiales Biocompatibles/química , Materiales Biocompatibles/efectos de la radiación , Línea Celular , Supervivencia Celular , Materiales Biocompatibles Revestidos , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Siloxanos/química , Siloxanos/efectos de la radiación , Sonicación , Espectrometría de Masa de Ion Secundario , Propiedades de SuperficieRESUMEN
A technique for improving the sensitivity of high mass molecular analysis is described. Three carbon species, fullerenes, single walled carbon nanotubes, and highly ordered pyrolytic graphite are introduced as matrices for the secondary ion mass spectrometry analysis of cyclodextrin (C(42)H(70)O(35), 1134 u). The fullerene and nanotubes are deposited as single deposition, and 10, 20, or 30 deposition films and cyclodextrin is deposited on top. The cyclodextrin parent-like ions and two fragments were analyzed. A 30 deposition fullerene film enhanced the intensity of cationized cyclodextrin with Na by a factor of 37. While the C(6)H(11)O(5) fragment, corresponding to one glucopyranose unit, increased by a factor of 16. Although fragmentation on fullerene is not suppressed, the intensity is twice as low as the parent-like ion. Deprotonated cyclodextrin increases by 100× and its C(8)H(7)O fragment by 10×. While the fullerene matrix enhances secondary ion emission, the nanotubes matrix film generates a basically constant yield. Graphite gives rise to lower intensity peaks than either fullerene or nanotubes. Scanning electron microscopy and atomic force microscopy provide images of the fullerene and nanotubes deposition films revealing flat and web structured surfaces, respectively. A "colliding ball" model is presented to provide a plausible physical mechanism of parent-like ion enhancement using the fullerene matrix.
