Outcomes after treatment of nonsevere gram-negative clinical mastitis with ceftiofur hydrochloride for 2 or 5 days compared with negative control.

A study was conducted at 3 commercial dairies in California to compare outcomes of treating nonsevere (mild and moderate) gram-negative (GN) clinical mastitis (CM) with intramammary (IMM) ceftiofur HCl (125 mg of ceftiofur HCl per tube) in either 2-d (SP2) or 5-d (SP5) treatment programs compared with nontreatment (CON). In addition, we contrasted results from cases classified as mild and moderate. Four hundred fifteen cases were included in the final dataset, including 135 CON, 133 SP2, and 147 SP5. Milk from quarters with CM was sampled for on-farm culture (OFC) to differentiate gram-positive (GP) and GN bacteria, with results known within 24 h. Those with GN infections were randomly assigned to experimental groups, while those with GP, mixed infections, and contaminated samples did not continue in the study and received standard farm therapy. For cows with GN infections, a sample was submitted for MALDI-TOF assay. Only nonsevere cases were enrolled, and all quarters yielded monocultures of GN species. Clinical scores were obtained 0, 1, 2, 3, 4, 5, 14, 21, and 28 ± 3 d relative to enrollment. Milk samples were collected from quarters 14, 21, and 28 ± 3 d after enrollment, and submitted for routine culture and, when appropriate, submitted to MALDI-TOF evaluation. For many response criteria, there were significant interactions between treatments and CM severity scores at the time of enrollment, with effectiveness of ceftiofur HCl treatment being more beneficial compared with CON as mastitis clinical severity increased. While most treatment responses were significant for animals with mild or moderate GN mastitis, the largest responses were noted among cows with moderate CM cases.

Small Performance Effects of a Practical Mixed-Methods Cooling Strategy in Elite Team Sport Athletes.

Purpose: The ingestion of ice slurry and application of ice towels can elicit favorable physiological, perceptual, and performance benefits when used individually; however, the combined use and effectiveness of these practical cooling strategies have not been assessed using a sport-specific performance test, based on actual match demands, in an elite team sport context. Methods: Ten non-heat acclimated elite male rugby sevens athletes undertook two cycling heat response tests (HRT) designed to be specific to the demands of rugby sevens in hot conditions (35°C, 80% rH). In a crossover design, the HRTs were conducted with (COOLING) and without (HOT) the combined use of internal (ice slushy ingestion) and external (application of ice towels to the head, neck, and face) pre- and per-cooling strategies. Physiological, perceptual, and performance variables were monitored throughout each HRT. Results: COOLING resulted in reductions in mean tympanic temperature (-0.4 ± 0.2°C; d = 1.18); mean heart rate (-5 ± 8 bpm; d = 0.53); thermal discomfort (-0.5 ± 0.9 AU; d = 0.48); and thirst sensation (-1.0 ± 1.1 AU; d = 0.61) during the HRT. COOLING also resulted in a small increase in 4-min time trial power output (by 7 ± 33 W, ~3%; d = 0.35) compared to HOT. Discussion: A combination of internal and external pre- and per-cooling strategies can result in a range of small physiological, perceptual, and performance benefits during a rugby sevens specific HRT, compared to undertaking no cooling. Practitioners should include such strategies when performing in hot conditions.

Practical application of a mixed active and passive heat acclimation protocol in elite male Olympic team sport athletes.

To investigate effectiveness and retention of heat acclimation (HA) integrated within an elite rugby sevens team training program, 12 elite male rugby sevens athletes undertook 10 days of mixed active/passive HA across 2 weeks of normal training. Physiological and performance variables were assessed using a sport-specific, repeated high-intensity heat-response test pre-HA; after 5 days (mid-HA) and 10 days (post-HA); and 16 days post-HA (decay). Resting, submaximal, and end-exercise core temperatures were lower at mid-HA (≤-0.26 °C; d ≥ -0.47), post-HA (≤-0.30 °C; d ≥ -0.72), and decay (≤-0.29 °C; d ≥ -0.56), compared to pre-HA. Sweat rate was greater at post-HA compared to pre-HA (0.3 ± 0.3 L·h-1; d = 0.63). Submaximal heart rate (HR) was lower at mid- (-9 ± 4 bpm; d = -0.68) and post-HA (-11 ± 4 bpm; d = -0.90) compared to pre-HA. Mean and peak 6 s power output improved at mid-HA (83 ± 52 W; 112 ± 67 W; d ≥ 0.47) and post-HA (125 ± 62 W; 172 ± 85 W; d ≥ 0.72) compared to pre-HA. Improvements in HR and performance persisted at decay (d ≥ 0.66). The initial 5 days of mixed-methods HA elicited many typical HA adaptations, with an additional 5 days eliciting further thermoregulatory, sudomotor, and performance improvements. Adaptations were well retained after 16 days of normal training, without any further heat stimulus. The trial was retrospectively registered with the Australian New Zealand Clinical Trials Registry (ACTRN12622000732785).

The relationship between physiological and performance variables during a hot/humid international rugby sevens tournament.

To characterise physiological responses to competing in an international rugby sevens tournament played in hot/humid conditions, core temperature (Tc) and Tc predictors were collected from 11 elite men's rugby sevens athletes competing in the Oceania sevens tournament in Suva, Fiji. Tc, body mass change, sweat electrolytes, playing minutes, total running distance, high speed running distance (HSD), psychrometric wet bulb temperature and exertional heat illness symptoms were collected pre, during and post games. Linear mixed-models were used to assess the effect of Tc predictors on post-game Tc, along with differences in Tc across measurement periods. Compared to baseline on both tournament days, mean Tc was higher during all between game (recovery) measures (all d >1.30, p <0.01). On both tournament days, eight athletes reached a post-game Tc >39.0°C, with several athletes reaching >39.0°C during warm-ups. Mean post-game Tc was related to playing minutes, total running distance, HSD, and post warm-up Tc (all p < 0.01). The Tc during warm-ups and games regularly exceeded those demonstrated to be detrimental to repeated sprint performance (> 39°C). Warm-up Tc represents the easiest predictor of post-game Tc to control via time/intensity modulation and the use of appropriate pre- and per-cooling strategies. Practitioners should be prepared to modulate warm-ups and other heat preparation strategies based on likely environmental conditions during hot/humid tournaments.

Identification of spatial intensity profiles from femtosecond laser machined depth profiles via neural networks.

Laser machining involves many complex processes, especially when using femtosecond pulses due to the high peak intensities involved. Whilst conventional modelling, such as those based on photon-electron interactions, can be used to predict the appearance of the surface after machining, this generally becomes unfeasible for micron-scale features and larger. The authors have previously demonstrated that neural networks can simulate the appearance of a sample when machined using different spatial intensity profiles. However, using a neural network to model the reverse of this process is challenging, as diffractive effects mean that any particular sample appearance could have been produced by a large number of beam shape variations. Neural networks struggle with such one-to-many mappings, and hence a different approach is needed. Here, we demonstrate that this challenge can be solved by using a neural network loss function that is a separate neural network. Here, we therefore present a neural network that can identify the spatial intensity profiles needed, for multiple laser pulses, to produce a specific depth profile in 5 µm thick electroless nickel.

Modelling laser machining of nickel with spatially shaped three pulse sequences using deep learning.

Femtosecond laser machining is a complex process, owing to the high peak intensities involved. Modelling approaches for the prediction of final sample quality based on photon-atom interactions are therefore challenging to extrapolate up to the microscale and beyond. The problem is compounded when multiple exposures are used to produce a final structure, where surface modifications from previous exposures must be taken into consideration. Neural network approaches allow for the automatic creation of a model that accounts for these challenging processes, without any physical knowledge of the processes being programmed by a specialist. We present such a network for the prediction of surface quality for multi-exposure femtosecond machining on a 5µm electroless nickel layer deposited on copper, where each pulse is uniquely spatially shaped using a spatial light modulator. This neural network modelling method accurately predicts the surface profile after three, sequential, overlapping exposures of dissimilar intensity patterns. It successfully reproduces such effects as the sub-diffraction limit machining feasible with multiple exposures, and the smoothing effect on edge-burr from previous exposures expected in multi-exposure laser machining.

Electroactive Amphiphiles for Addressable Supramolecular Nanostructures.

In this focus review we aim to highlight an exciting class of materials, electroactive amphiphiles (EAAs). This class of functional amphiphilic molecules has been the subject of sporadic investigations over the last few decades, but little attempt has been made to date to gather or organise these investigations into a logical fashion. Here we attempted to gather the most important contributions, provide a framework in which to discuss them, and, more importantly, point towards the areas where we believe these EAAs will contribute to solving wider scientific problems and open new opportunities. Our discussions cover materials based on low molecular weight ferrocenes, viologens and anilines, as well as examples of polymeric and supramolecular EAAs. With the advances of modern analytical techniques and new tools for modelling and understanding optoelectronic properties, we believe that this area of research is ready for further exploration and exploitation.

Ballistic and snake photon imaging for locating optical endomicroscopy fibres.

We demonstrate determination of the location of the distal-end of a fibre-optic device deep in tissue through the imaging of ballistic and snake photons using a time resolved single-photon detector array. The fibre was imaged with centimetre resolution, within clinically relevant settings and models. This technique can overcome the limitations imposed by tissue scattering in optically determining the in vivo location of fibre-optic medical instruments.

Endoscopic sensing of alveolar pH.

Previously unobtainable measurements of alveolar pH were obtained using an endoscope-deployable optrode. The pH sensing was achieved using functionalized gold nanoshell sensors and surface enhanced Raman spectroscopy (SERS). The optrode consisted of an asymmetric dual-core optical fiber designed for spatially separating the optical pump delivery and signal collection, in order to circumvent the unwanted Raman signal generated within the fiber. Using this approach, we demonstrate a ~100-fold increase in SERS signal-to-fiber background ratio, and demonstrate multiple site pH sensing with a measurement accuracy of ± 0.07 pH units in the respiratory acini of an ex vivo ovine lung model. We also demonstrate that alveolar pH changes in response to ventilation.

Fetal domoic acid exposure affects lateral amygdala neurons, diminishes social investigation and alters sensory-motor gating.

Domoic acid (DA) is an algal neurotoxin that accumulates in marine fish and shellfish. DA can move across the placenta and concentrate in amniotic fluid, which can be swallowed during late gestation. DA also transfers to infants via milk. Preclinical studies to determine effects of developmental DA expose have primarily involved DA exposure during the postnatal period and little is known about late CNS effects following prenatal DA. In the present study, we tested the hypothesis that prenatal exposure of FVB mice to low levels of DA would result in diminished social interaction and sensory motor gating associated with alterations in parvalbumin immunoreactivity in relevant brain regions undergoing development during and following DA exposure. In addition to parvalbumin, we stained with NeuN for a neuronal specific nuclear protein to determine if neuronal loss followed prenatal DA exposure. A single moderate dose of DA administered during gestation produces diminishes social investigation and alters sensorimotor gating, behavioral effects more pronounced in males than females. These behavioral changes were associated with discrete alterations in the parvalbumin-positive subtype of GABAergic neurons in the dentate gyrus and lateral amygdala.

Cognitive rehabilitation group intervention for breast cancer survivors: results of a randomized clinical trial.

PURPOSE: We conducted a randomized clinical trial evaluating the efficacy of a cognitive rehabilitation (CR) intervention compared with a wait list (WL) control condition on cognitive complaints, neuropsychological and brain functioning in breast cancer survivors (BCS). METHODS: The small group intervention of five sessions included psychoeducation and cognitive exercises. ELIGIBILITY: Disease-free BCS with cognitive complaints, diagnosed with stage I, II or III breast cancer, completed primary treatment 18 months to 5 years earlier. Neurocognitive test data and cognitive complaints on the Patient's Assessment of Own Functioning Inventory (PAOFI) were assessed at baseline (T1), immediately post-intervention (T2), and 2 months later (T3). A subgroup of participants underwent resting state quantitative electroencephalography (qEEG) at all three assessment time points. RESULTS: Forty-eight participants [mean age (SD) 53.8 (8.2)] completed T1 assessments, and 29 participants had analyzable qEEG data. The CR group improved significantly over time compared with the WL group on PAOFI total and memory scores (both p = .01) and on Rey Auditory Verbal Learning Test (RAVLT) total (trials I-V) (p = .02) and RAVLT delayed recall (p = .007) scores. On qEEG, the CR group showed a significant decrease in delta 'slow wave' power (p = .02) and an increase in the frontal distribution of alpha power (p = .04) from T1 to T2. CONCLUSIONS: BCS in the CR group showed immediate and sustained improvements in self-reported cognitive complaints and memory functioning on neurocognitive testing. Results of the qEEG substudy provide some support for neurophysiological changes underlying the intervention. Copyright © 2015 John Wiley & Sons, Ltd.

Laser-induced photo-polymerisation for creation of paper-based fluidic devices.

Paper-based microfluidics is a rapidly progressing inter-disciplinary technology driven by the need for low-cost alternatives to conventional point-of-care diagnostic tools. For transport of reagents/analytes, such devices often consist of interconnected hydrophilic fluid-flow channels that are demarcated by hydrophobic barrier walls that extend through the thickness of the paper. Here, we present a laser-based fabrication procedure that uses polymerisation of a photopolymer to produce the required fluidic channels in paper. Experimental results showed that the structures successfully guide the flow of fluids and allow containment of fluids in wells, and hence the technique is suitable for fabrication of paper-based microfluidic devices. The minimum width for the hydrophobic barriers that successfully prevented fluid leakage was ~120 µm and the minimum width for the fluidic channels that can be formed was ~80 µm, the smallest reported so far for paper-based fluidic patterns.

Do stressful events account for the link between socioeconomic status and mental health?

BACKGROUND: The purpose of this study was to prospectively examine the relationships between socioeconomic status (SES), demographic variables and mental health and to determine whether number of life stressors mediated these relationships. METHODS: Wave 1 (2001-02) and 2 (2004-05) data from the National Epidemiologic Survey on Alcohol and Related Conditions (NESARC; n = 34,459) were used in the current study. Controlling for wave 1 mental health, a mediation model was tested to determine the relative impact of socioeconomic and demographic variables (all measured at wave 1) on mental health 3 years later (wave 2). The number of life stressors experienced in the 12 months prior to wave 1, assessed at wave 1, was evaluated as the mediator. RESULTS: Findings indicated that SES, age, race/ethnicity, gender and marital status independently predicted changes in mental health ratings at wave 2. In addition, the number of life stressors mediated the relation between socioeconomic and demographic variables and mental health. Exposure to life stressors helps to explain commonly reported socioeconomic and demographic disparities in mental health. CONCLUSIONS: Findings may suggest that reducing exposure to stressors and/or improving coping with life stressors may both improve mental health and reduce health disparities.

An independent audit of the Australian food industry's voluntary front-of-pack nutrition labelling scheme for energy-dense nutrition-poor foods.

BACKGROUND/OBJECTIVE: Since 2006, the Australian food industry has promoted its front-of-pack (FOP) food labelling system-the Daily Intake Guide (DIG)-as a success story of industry self-regulation. With over 4000 products already voluntary featuring the DIG, the industry argues that government regulation of FOP nutrition labelling is simply unnecessary. However, no independent audit of the industry's self-regulation has ever been undertaken and we present the first such Australian data. SUBJECTS/METHODS: Energy-dense nutrient-poor (EDNP) snacks were audited at nine Australian supermarkets, including biscuits, candy, ice creams, chocolates, crisps, sports drinks, energy drinks, flavoured milks, sweetened juices and soft drinks. In these categories nutrition labels were recorded for 728 EDNP products in various packaging sizes. RESULTS: The DIG was displayed on 66% of audited EDNP products but most of these (75%) did not report saturated fat and sugar content. Only generic supermarket EDNP products were likely to display saturated fat and sugar content, compared with very few branded products (48% vs 4%, P<0.001). Branded products not displaying fat and sugar content contained on average 10-times more saturated fat than those displaying such (10% vs 1% DI, P<0.001) and nearly twice as much sugar (21 vs 13% DI, P<0.05). CONCLUSIONS: Most Australian manufacturers of EDNP products have adopted the DIG; consistent with industry claims of widespread adoption, but almost all still avoid displaying the high saturated fat and sugar content of their products by opting for the 'energy alone' option, violating the industry's own voluntarily guidelines and highlighting serious weaknesses with the industry's self-regulation.

Intimal hyperplasia: slow but deadly.

Intimal hyperplasia is the leading cause of long-term failure in coronary artery bypass vein grafting, coronary artery stenting, angioplasty, arteriovenous fistula for dialysis, and allograft transplantation. Intimal hyperplasia is a product of vascular smooth muscle cell proliferation, migration through the internal elastic lamina, and deposition of extracellular matrix proteins driven by growth factors in the vasculature. This vascular pathology results in a progressive diminution of the vessel lumen and serves as a site for thrombosis and atherosclerotic lesions. A key cell type in the initiation of intimal hyperplasia is the vascular endothelial cell, which appears to have down-stream effects on the vascular smooth muscle proliferation and migration. Currently, the only means available for prevention of intimal hyperplasia is through inhibition of mammalian target of rapamycin (mTOR) with the immunosuppressant rapamycin. mTOR integrates up-stream signals from growth factors such as IL-2 and senses the cellular nutrient and energy levels and redox status. This presentation will discuss the potential means of preserving the vascular endothelial cell and, thereby, reducing the development of intimal hyperplasia in our open-heart surgical patients.

Assessing resistance against macrocyclic lactones in gastro-intestinal nematodes in cattle using the faecal egg count reduction test and the controlled efficacy test.

The main objective of the present study was to evaluate the accuracy of the faecal egg count reduction test (FECRT) to assess the resistance status of ivermectin (IVM)-resistant isolates of the cattle nematodes Ostertagia ostertagi and Cooperia oncophora, using the controlled efficacy test (worm counts) as a reference. The second objective was to investigate whether both IVM-resistant isolates showed side-resistance against moxidectin (MOX) under controlled conditions. Thirty male Holstein calves were experimentally infected with 25,000 L3 of an IVM-resistant O. ostertagi isolate and 25,000 L3 of an IVM-resistant C. oncophora isolate. Twenty-eight days later the calves were randomly divided into 2 treatment groups and 1 untreated control group. Animals in groups 1 and 2 received MOX (Cydectin(®) 1%, Pfizer) and IVM (Ivomec(®) 1%, Merial) respectively, by subcutaneous injection at a dose rate of 0.2mg/kg bodyweight. Faecal samples were collected 7 and 14 days after treatment and animals were necropsied 14/15 days post-treatment. Both the FECRT and the controlled efficacy test demonstrated that the O. ostertagi and C. oncophora isolates were resistant against IVM, with efficacies below 90%. The IVM-resistant O. ostertagia isolate was still susceptible to MOX treatment, as shown by over 99% reduction in egg counts and worm burden. The FECRT suggested borderline resistance against MOX in the IVM-resistant C. oncophora isolate, with egg count reductions between 97% (95% CI: 76; 100) at day 7 and 86% (95% CI: 49; 96) at day 14. However, the controlled efficacy test clearly showed MOX-resistance, with a decrease of only 31% (95% CI: -12; 57) in C. oncophora worm numbers. After MOX treatment, a significantly lower number of eggs per female C. oncophora worms was counted compared to the control group (43% reduction). Due to this reduced fecundity, the FECRT may fail to detect MOX-resistance.

The effect of retail cigarette pack displays on unplanned purchases: results from immediate postpurchase interviews.

OBJECTIVE: To assess the influence of point-of-sale (POS) cigarette displays on unplanned purchases. METHODS: Intercept interviews were conducted with customers observed purchasing cigarettes from retail outlets featuring POS cigarette displays. Measures included intention to purchase cigarettes prior to entering the store, unprompted and prompted salience of POS tobacco displays, urge to buy cigarettes as a result of seeing the POS display, brand switching and support for a ban on POS cigarette displays. RESULTS: In total, 206 daily smokers aged 18-76 years (90 male, 116 female) were interviewed. Unplanned cigarette purchases were made by 22% of participants. POS displays influenced nearly four times as many unplanned purchases as planned purchases (47% vs 12%, p<0.01). Brand switching was reported among 5% of participants, half of whom were influenced by POS displays. Four times as many smokers were supportive of a ban on POS tobacco displays than unsupportive (49% vs 12%), and 28% agreed that such a ban would make it easier to quit. CONCLUSIONS: POS tobacco displays act as a form of advertising even in the absence of advertising materials. They stimulate unplanned cigarette purchases, play an important role in brand selection and tempt smokers trying to quit. This justifies removing POS tobacco displays from line of sight-something that very few smokers in our sample would object to.

Semantic processing in "associative" false memory.

We studied the semantic properties of a class of illusions, of which the Deese/Roediger-McDermott (DRM) paradigm is the most prominent example, in which subjects falsely remember words that are associates of studied words. We analyzed DRM materials for 16 dimensions of semantic content and assessed the ability of these dimensions to predict interlist variability in false memory. For the more general class of illusions, we analyzed pairs of presented and unpresented words that varied in associative strength for the presence of these same 16 semantic properties. DRM materials proved to be exceptionally rich in meaning, as indexed by these semantic properties. Variability in false recall, false recognition, and backward associative strength loaded on a single semantic factor (familiarity/meaningfulness), whereas variability in true recall loaded on a quite different factor (imagery/concreteness). For word association generally, 15 semantic properties varied reliably with forward or backward association between words. Implications for semantic versus associative processing in this class of illusions, for dual-process theories, and for semantic properties of word associations are discussed.

A trial of spontaneous breathing to determine the readiness for extubation in very low birth weight infants: a prospective evaluation.

Extubation failure in premature infants is common. A spontaneous breathing trial (SBT) was prospectively evaluated to determine timing of extubation. Compared with historical controls, infants were extubated at significantly higher ventilator rates and airway pressures using the SBT. No differences in rates of bronchopulmonary dysplasia or duration of ventilation were seen.

Alterations in growth plate and articular cartilage morphology are associated with reduced SOX9 localization in the magnesium-deficient rat.

Insufficient dietary magnesium (Mg) intake has been associated with low bone mass in humans,and recent basic science studies have indicated that this bone loss may be secondary to increased release of substance P and TNFc Much less is known about the effects of low Mg intake on cartilage. We have evaluated growth plate and articular cartilage in rats following a 6 month dietary Mg restriction. Histomorphometry demonstrated significantly decreased distal femur articular cartilage chondrocyte density and decreased tibial growth plate width in experimental animals compared to controls. Growth plates of Mg-restricted animals showed reduced chondrocyte column formation. Extracellular matrix of both articular cartilage and growth plates in experimental animals contained reduced amounts of proteoglycans. Immunolocalization of Sox9 was decreased in both articular and growth plate cartilage in experimental animals compared to controls, suggesting that reduced Mg intake causes cartilage changes that may be secondary to reduced levels of the SOX9 transcription factor.