[CHARACTERISTICS OF LEVOFLOXACIN AND ITS CLINICAL APPLICATION (REVIEW)].

We analyzed of the effectiveness of levofloxacin in various diseases, the sensitivity of microorganisms to it and side effects. LF does not show carcinogenic, mutagenic and teratogenic activity. It is used effectively in the treatment of infections of the respiratory and genitourinary tract, skin and soft tissues, for the prevention of complications in surgical practice, as well as infections of many other localizations. Levofloxacin has a wide spectrum of action, including most gram-positive and gram-negative pathogens, including intracellularly located, unlike other fluoroquinolones, highly active against gram-positive cocci, including pneumococci which are resistant to penicillin, erythromycin. In addition, levofloxacin is more active against atypical pathogens. Doctors should clearly justife the appointment of LF in an adequate dose in each case, in order to avoid or reduce the occurrence of resistance of pathogens to it.

THE INFLUENCE OF CONTRACEPTION ON VAGINAL MICROBIOCENOSIS CONDITION.

Aim - in complex to evaluate different types of female vaginal biocenosis on the background of contraceptives usage in order to detect implant-associated infection. Clinical status, features of vaginal microflora and formation of biofilms were assessed in 64 women of reproductive age, who had inert intrauterine device (IUD) of the second generation for a period of from 5 months to 8 years. pH of vaginal secretion was measured by the test strips CITOLAB (Farmasko, Ukraine). The condition of vaginal biocenosis was assessed according to «Femoflor-Screen¼, polymerase chain reaction in real time. The formation of biofilms was explored with the study of the ability of pure culture Gardnerellaе to adhesion on the surface of microplates and the addition of crystal violet indicator. Optical density was measured with the photometer «Multiskan EX-355¼ (Labsystems, China). These vaginal micribiota of the patients in control group were inconsistent with microbiocenosis of I and II type (normocenosis, intermediate type). II type of the vagina normocenosis was found in 29,4 % patients with long usage of contraception up to 12 month. III type of vaginal microbiocenosis was observed in 55, 8 % of examined patients within the duration of IUD use from 2 till 5 years. Microbiocenosis of women with IV type of smear was inconsistent with non-specific or hybrid vaginitis (IUD duration more than 5 years). Changes of total bacterial exudates, detection of opportunistic or pathogenic flora, especially for mixed bacterial associations for women with intra-uterine devices gives rise to vaginal biofilm formation. The type of vaginal biocenosis depends on the duration of IUC use. рН level of vaginal secretion, which conforms to the quantitative evaluation of microbiocenosis and the availability of lactobacteria in it can be used as an indicator for condition of vaginal biocenosis. pН level of vaginal secretion can be a screen control of vaginal biocenosis condition during intrauterine devices stay for the control of its durability in the uterus.

Edible larvae and pupae of honey bee (Apis mellifera): Odor and nutritional characterization as a function of diet.

Future protein demand is expected to rise with global population growth. In this study a comprehensive sensorial analysis of the odor of honey bee (Apis mellifera) larvae and pupae as function of their diet (with and without added sugar solution) was performed, as well as nutritional values and antioxidant activity analysis. Honey bee brood powder is a potentially valuable nutritional source with 20-25% protein (dry matter basis), high antioxidant activity and polyphenol content. Main volatile compounds detected using GC-MS with HS-SPME injection were odorless pheromones that represented differences between larvae and pupae. The determined active odor compounds were 2- and 3-methylbutanal, diacetyl, nonanal, dimethyl sulfide and ocimene. A trained sensory panel described honey bee brood aroma profile mainly with buttery and milky attributes, with different life stages and diets giving similar profiles. Such studies can be useful for future development of food products with desired nutritional and sensorial characteristics.

Effects of radiation damage in studies of protein-DNA complexes by cryo-EM.

Nucleic acids are responsible for the storage, transfer and realization of genetic information in the cell, which provides correct development and functioning of organisms. DNA interaction with ligands ensures the safety of this information. Over the past 10 years, advances in electron microscopy and image processing allowed to obtain the structures of key DNA-protein complexes with resolution below 4Å. However, radiation damage is a limiting factor to the potentially attainable resolution in cryo-EM. The prospect and limitations of studying protein-DNA complex interactions using cryo-electron microscopy are discussed here. We reviewed the ways to minimize radiation damage in biological specimens and the possibilities of using radiation damage (so-called 'bubblegrams') to obtain additional structural information.