Soil-dwelling grub larvae of Protaetia brevitarsis biodegrade polystyrene: Responses of gut microbiome and host metabolism.

Plastic pollution poses a significant threat to terrestrial ecosystems, yet the potential for soil fauna to contribute to plastic biodegradation remains largely unexplored. In this study, we reveal that soil-dwelling grubs, Protaetia brevitarsis larvae, can effectively biodegrade polystyrene (PS) plastics. Over a period of 4 weeks, these grubs achieved a remarkable 61.5 % reduction in PS foam mass. This biodegradation was confirmed by the depolymerization of ingested PS, formation of oxidative functional groups, noticeable chemical modifications, and an increase of δ13C of residual PS in frass. Additionally, antibiotic treatment to suppress gut microbes led to variations in the biodegradation process. PS ingestion induced a significant shift in the gut microbiome, promoting the growth of degradation-related bacteria such as Promicromonosporaceae, Bacillaceae, and Paenibacillaceae. Furthermore, the digestion of plastic triggered extensive metabolomic reprogramming of grubs' intestines, enhancing redox capabilities and facilitating PS biodegradation. These results indicate that responsive adaptation of both the gut microbiome and the host's intestinal metabolism contributes to PS degradation. Collectively, these findings demonstrate P. brevitarsis larvae's capability to alleviate soil plastic pollution, and highlight the potential of researching soil fauna further for sustainable plastic waste management solutions.

Polymer chain extenders induce significant toxicity through DAF-16 and SKN-1 pathways in Caenorhabditis elegans: A comparative analysis.

Polymer chain extenders, commonly used in plastic production, have garnered increasing attention due to their potential environmental impacts. However, a comprehensive understanding of their ecological risks remains largely unknown. In this study, we employed the model organism Caenorhabditis elegans to investigate toxicological profiles of ten commonly-used chain extenders. Exposure to environmentally relevant concentrations of these chain extenders (ranging from 0.1 µg L-1 to 10 mg L-1) caused significant variations in toxicity. Lethality assays demonstrated the LC50 values ranged from 92.42 µg L-1 to 1553.65 mg L-1, indicating marked differences in acute toxicity. Sublethal exposures could inhibit nematodes' growth, shorten lifespan, and induce locomotor deficits, neuronal damage, and reproductive toxicity. Molecular analyses further elucidated the involvement of the DAF-16 and SKN-1 signaling pathways, as evidenced by upregulated expression of genes including ctl-1,2,3, sod-3, gcs-1, and gst-4. It implicates these pathways in mediating oxidative stress and toxicities induced by chain extenders. Particularly, hexamethylene diisocyanate and diallyl maleate exhibited markedly high toxicity among the chain extenders, as revealed through a comparative analysis of multiple endpoints. These findings demonstrate the potential ecotoxicological risks of polymer chain extenders, and suggest the need for more rigorous environmental safety assessments.

Removal of microplastics from water by coagulation of cationic-modified starch: An environmentally friendly solution.

Microplastics (MPs) pose a potential risk to aquatic ecosystems, and there is a growing demand to alleviate the contamination of MPs. Here, we introduce cationic-modified starch (CS) as an eco-friendly bio-coagulant for removing MPs from water. CS with varying degrees of substitution was synthesized and characterized, and its performance in removing MPs was evaluated under different MP sizes, types, and aging, as well as various water conditions. The results indicated that CS efficiently removed MPs, achieving an average removal rate of 65.33 % for polystyrene particles, with higher removal rates for larger, high-density, and aged MPs. The efficiency of CS remained consistent across a wide range of water pH values, but was significantly reduced in the presence of kaolin clay or/and humic acid. The removal efficiency of CS for MPs was enhanced by the non-ionic surfactant, Tween 20, but inhibited by the anionic surfactant, cetyltrimethylammonium bromide. In addition, CS could concurrently remove both MPs and phenanthrene, as a typical water contaminant. Moreover, the applicability of CS was demonstrated in natural water samples from the Ecological Demonstration Zone of the Yangtze River Delta, China, with an average removal rate of 60.13 ± 3.15 %. Taken together, this study offers an environmentally friendly and cost-effective approach for the removal of MPs from water, demonstrating CS has significant application potential as a sustainable solution to mitigate microplastic pollution.

Microfiber-loaded bacterial community in indoor fallout and air-conditioner filter dust.

Microfibers (MFs) are widely existed in indoor air; however, characteristic of microbiota on MFs is largely unknown. In this study, air-borne MFs were collected from fallout or air-conditioner (AC) filter dust in three types of indoor space including living room, dormitory and office. Both plastic and natural MFs were identified by Fourier transform infrared spectroscopy. Ultramicroscopic observation showed dense biofilms adhering on surfaces of MFs. Fallout MFs contained more bacteria but fewer fungi than MFs from AC filter dust. MFs-loaded bacteria were of highest abundance in living rooms, following dormitories and offices. Bacterial community and its diversity were further analyzed by 16S rRNA High-throughput sequencing. Up to 4540 of bacterium OTUs were shared in these MFs samples, unique OTUs in fallout and AC filter samples accounting for 26.3 % and 25.7 % of the total. Compared to MFs fallout, AC filter MFs contained more species of pathogenic bacteria, such as Betaproteobacteriales and Ralstonia, with obviously different ß-diversity between two groups. Phenotypic analysis showed that fallout and AC filter MFs bacteria presented high index values of film formation, oxidative stress tolerance and potential pathogenicity. Overall, these results suggest that abundant bacteria including pathogen can be loaded on MFs, and would pose health risks through delivery of indoor MFs.

Prominent toxicity of isocyanates and maleic anhydrides to Caenorhabditis elegans: Multilevel assay for typical organic additives of biodegradable plastics.

Biodegradable plastics (BDP) are increasingly applied; however, there has been of concerns about their environmental safety, especially from nondegradable additive compositions. Until now, data of ecotoxicity of BDP additives is scarce. Here, nematode C. elegans was used to comparatively evaluate toxicity of an isocyanate additive, i.e., Hexamethylene diisocyanate (HDI), a maleic anhydride, i.e., Diallyl maleate (DIM), and other four BDP organic additives. These additives caused lethality of nematodes at µg L-1 level, of lowest LC50 value of HDI/DIM. Uniform exposure to these additives resulted in various degrees of inhibitions in body volumes and longevity, indicating developmental toxicity. Moreover, BDP additives induced significant elevations of gst-4 expression, especially mean 123.54 %/234.29 % increase in HDI/DIM group, but reduced ges-1 expression, which indicates oxidative damages and mitochondrial dysfunction. BDP additives further caused inhibition in locomotor and food intake/excretion behavior, and related damages of glutamatergic neurons and GABAergic neurons, indicating their neurotoxicity. We found HDI and DIM presented relatively strong effects on susceptible endpoints including lethality, gst-4, mean lifespan, food intake and excretion behavior. Overall, this study suggests prominent ecotoxic risk of isocyanates and maleic anhydrides as BDP additives, which is significant for the selection of environmentally friendly BDP additives.

Degradation of polylactic acid/polybutylene adipate films in different ratios and the response of bacterial community in soil environments.

Biodegradable plastic mulch film (BDM) is an environmentally friendly alternative to conventional polyethylene mulch, and has been growingly used in agriculture. However, practical degradation performance of BDM, especially the widely used type of blended polylactic acid (PLA)/polybutylene adipate (PBAT) in different ratios, and microbial alteration in soil environments, remain largely unrevealed. In this study, four types of BDM blended with 40-80% PLA and 20-60% PBAT were comparatively investigated through microcosm soil incubation experiments for 105 days, and combined with conditions of different soil moisture or pH. Microbiome within film-surrounding soil were assayed using 16 S rRNA high-throughput sequencing. Results showed a trend of increasing degradation efficiency with the increase of PLA proportion, and 70% PLA and 30% PBAT group presented the highest weight loss rate, i.e., 60.16 ± 5.86%. In addition, degradation and aging of PLA/PBAT varied among different soil moisture and pH values. A moderate moisture, i.e., 60% and a neutral pH7.0 caused significantly high degradation efficiency compared to other moisture or pH conditions. Moreover, bacterial abundance and community structure in the surrounding soil were related to soil moisture and pH. PLA/PBAT incubation treatment induced a remarkable increase in abundance of degradation-related species Pseudomonas and Sphingomonas. Bacterial richness and diversity in soil correspondingly respond to ratio-different PLA/PBAT's degradation under moisture/pH-different conditions through a redundancy analysis. Altogether, these findings indicate that practical degradation of PLA/PBAT film is closely related to soil environments and bacterial community. It is significant for the application of biodegradable plastics in agriculture on the perspective of soil sustainability.

Size/shape-dependent migration of microplastics in agricultural soil under simulative and natural rainfall.

Agricultural soil is a sink of microplastics (MPs) in the environment. MPs in topsoil can be transferred deeply or into surrounding water by rainfall. However, little is known about rainfall-induced migration pattern of different MPs in agricultural soil. In this study, soil leaching experiments of 21 d were performed on Nile red-stained size-different polyethylene terephthalate (PET) particles, and shape-different polyethylene (PE) MPs under simulated or natural rainfall. Results showed that simulated rainfall of 5-25 mm/d caused intensity-dependent migration of MPs in horizontal and vertical directions. Maximum migration depth of MP particles arrived up to 4-7 cm. Rise of soil slopes could significantly increase horizontal mobility of MPs. Comparatively, natural rainfall of similar intensity caused relatively high mobility of MPs. Moreover, under both simulative and natural rainfall, mobility of MPs presented size/shape-different characteristics. Comparatively, small-size MPs (especially <1 mm) showed relatively high mobility in horizontal or vertical direction, and had high-frequency presence in runoff water. Of four MPs' shapes, fiber and film had relatively high mobility in comparison to particles. These results indicate that rainfall can cause size/shape-dependent migration of MPs in agricultural soil. It suggests size/shape-different environment fate of MPs, and provides a reference for MP control.