Biowaste management by hydrothermal carbonization and anaerobic co-digestion: Synergistic effects and comparative metagenomic analysis.

The feasibility of anaerobic co-digestion in semicontinuous mode of two major urban biowaste, food waste (FW) and garden and park waste (GPW) (75 % FW and 25 % GPW) as well as the co-digestion of FW with the process water originated from the hydrothermal carbonization of GPW (95 % FW and 5 % process water), both on a COD basis, has been assessed. The effect of varying organic loading rate (OLR) from 1.5 to 3.5 g COD/L·d on methane yield, gross energy recovery, and microbiome population was evaluated. For comparison, anaerobic digestion of FW was also conducted to determine the best strategy for sustainable biowaste management. This study showed an optimal OLR of 2.5 g COD/L·d. Acetic and propionic acid content increased as OLR raised for each condition studied, while methane yield decreased at the highest OLR tested indicating overloading of the system. The anaerobic co-digestion of FW and process water showed a 10 % increase on methane production compared to anaerobic digestion of FW (324 vs. 294 mL CH4 STP/L·d). Moreover, it enhances the process due to a greater abundance and diversity of hydrolytic and acidogenic bacteria belonging to Bacterioidota, Firmicutes, and Chloroflexi phyla, as well as promotes the hydrogenotrophic pathway under higher propionic concentrations which is not usually favoured for methane production. The integration of hydrothermal carbonization of GPW with the anaerobic co-digestion of 95 % FW and 5 % of process water results in the highest potential energy recovery and could be a good strategy for sustainable management of urban biowaste.

Study of two approaches for the process water management from hydrothermal carbonization of swine manure: Anaerobic treatment and nutrient recovery.

Hydrothermal carbonization (HTC) is a promising alternative to transform biomass waste into a solid carbonaceous material (hydrochar) and a process water with potential for material and energy recovery. In this study, two alternatives for process water treatment by conventional and acid-assisted HTC of swine manure are discussed. Process water from conventional HTC at 180 °C showed high biodegradability (55% COD removal) and methane production (∼290 mL STP CH4 g-1 CODadded) and the treatment in an upflow anaerobic sludge blanket reactor allowed obtaining a high methane production yield (1.3 L CH4 L-1 d-1) and COD removal (∼70%). The analysis of the microbiota showed a high concentration of Synergistota and Firmicutes phyla, with high degradation of organic nitrogen-containing organic compounds. Acid-assisted HTC proved to be a viable option for nutrient recovery (migration of 83% of the P to the process water), which allowed obtaining a solid salt by chemical precipitation with Mg(OH)2 (NPK of 4/4/0.4) and MgCl2 (NPK 8/17/0.5), with a negligible content of heavy metals. The characteristics of the precipitated solid complied with the requirements of European Regulation (2019)/1009 for fertilizers and amendments in agricultural soils, being a suitable alternative for the recycling of nutrients from wastes.

Swine manure management by hydrothermal carbonization: Comparative study of batch and continuous operation.

Hydrothermal carbonization (HTC) is considered a promising technology for biomass waste management without pre-drying. This study explores the potential for swine manure management by comparing batch and continuous processes, emphasizing the benefits of the continuous mode, particularly for its potential full-scale application. The continuous process at low temperature (180 °C) resulted in a hydrochar with a lower degree of carbonization compared to the batch process, but similar characteristics were found in both hydrochars at higher operating temperatures (230-250 °C), such as C content (∼ 52 wt%), fixed carbon (∼ 24 wt%) and higher calorific value (21 MJ kg-1). Thermogravimetric and combustion analyses showed that hydrochars exhibited characteristics suitable as solid biofuels for industrial use. The process water showed a high content of organic matter as soluble chemical oxygen demand (7-22 g L-1) and total organic carbon (4-10 g L-1), although a high amount of refractory species such as N- and O-containing long aromatic compounds were detected in the process water from the batch process, while the process water from the continuous process presented more easily biodegradable compounds such as acids and alcohols, among others. The longer time required to reach operating temperature in the case of the batch system (longer heating time to reach operating temperature) resulted in lower H/C and O/C ratios compared to hydrochar from the continuous process. This indicates that the dehydration and decarboxylation reactions of the feedstock play a more important role in the batch process. This study shows the efficiency of the continuous process to obtain carbonaceous materials suitable for use as biofuel, providing a solution for swine manure management.

Co-hydrothermal carbonization of swine manure and lignocellulosic waste: A new strategy for the integral valorization of biomass wastes.

Co-hydrothermal carbonization (co-HTC) is a promising strategy to improve hydrothermal carbonization (HTC) of low-quality wastes. HTC of swine manure (SM), with high N (2.9 wt%), S (0.7 wt%) and ash (22.6 wt%) contents, as well as low C (35.6 wt%) and higher heating value (HHV; 14.3 MJ kg-1), resulted in a hydrochar with unsuitable characteristics as a solid fuel. Co-HTC of SM and garden and park waste (GPW) improved hydrochar properties (C content (43 - 48 wt%) and HHV (18 - 20 MJ kg-1), and decreased N (∼2 wt%), S (<0.3 wt%) and ash (<15 wt%) content. A high GPW ratio (>50 wt%) during co-HTC resulted in a hydrochar similar to that obtained from GPW. The co-HTC increased nutrient migration to the process water, which allowed the precipitation of salt with high P (7.8 wt%) and negligible heavy metal content. Anaerobic digestion of co-HTC process water allowed high organic matter removal (up to 65%), and methane production (315 - 325 mL CH4 g-1CODadded). Gross energy recovery by HTC and anaerobic digestion was 5 - 6-fold higher than anaerobic treatment of feedstocks. Therefore, co-HTC of SM and GPW with a ratio > 50% GPW proved to be a suitable approach to valorize and manage SM and obtain value-added products (hydrochar, mineral fertilizer and methane).

Critical review of technologies for the on-site treatment of hospital wastewater: From conventional to combined advanced processes.

This review aims to assess different technologies for the on-site treatment of hospital wastewater (HWW) to remove pharmaceutical compounds (PhCs) as sustances of emerging concern at a bench, pilot, and full scales from 2014 to 2020. Moreover, a rough characterisation of hospital effluents is presented. The main detected PhCs are antibiotics and psychiatric drugs, with concentrations up to 1.1 mg/L. On the one hand, regarding the presented technologies, membrane bioreactors (MBRs) are a good alternative for treating HWW with PhCs removal values higher than 80% in removing analgesics, anti-inflammatories, cardiovascular drugs, and some antibiotics. Moreover, this system has been scaled up to the pilot plant scale. However, some target compounds are still present in the treated effluent, such as psychiatric and contrast media drugs and recalcitrant antibiotics (erythromycin and sulfamethoxazole). On the other hand, ozonation effectively removes antibiotics found in the HWW (>93%), and some studies are carried out at the pilot plant scale. Even though, some families, such as the X-ray contrast media, are recalcitrant to ozone. Other advanced oxidation processes (AOPs), such as Fenton-like or UV treatments, seem very effective for removing pharmaceuticals, Antibiotic Resistance Bacteria (ARBs) and Antibiotic Resistance Genes (ARGs). However, they are not implanted at pilot plant or full scale as they usually consider extra reactants such as ozone, iron, or UV-light, making the scale-up of the processes a challenging task to treat high-loading wastewater. Thus, several examples of biological wastewater treatment methods combined with AOPs have been proposed as the better strategy to treat HWW with high removal of PhCs (generally over 98%) and ARGs/ARBs (below the detection limit) and lower spending on reactants. However, it still requires further development and optimisation of the integrated processes.

Energy recovery from garden and park waste by hydrothermal carbonisation and anaerobic digestion.

Hydrothermal carbonisation (HTC) can transform wet lignocellulosic biomass, which is not considered an effective biofuel for energy production at the industrial level, into a carbonaceous product called hydrochar (HC) that is suitable for combustion and a process water (PW). PW is an interesting by-product that can be valorised for biogas production via anaerobic digestion (AD). This study presents a new approach for the valorisation of garden and park wastes (GPW) by integrating HTC to generate HC for energy production, while PW is subjected to AD for biogas production. The hydrothermal treatment was performed at 180, 210, and 230 °C, yielding HC with improved physicochemical properties, such as an elevated higher heating value (21-25 MJ kg-1); low ash (<5 wt.%), nitrogen (1.3 wt.%), and sulphur (0.2 wt.%) contents; better fuel ratio (0.4-0.6); and a broad comprehensive combustibility index (8.0×10-7 to 9.6×10-7 min-2 °C-3). AD of the generated PW was conducted under mesophilic conditions (35 °C), resulting in a methane production in the range of 253-326 mL g-1 CODadded and COD removal of up to 65%. The combination of HTC and AD allowed the recovery of 91% and 94% of the energy content feedstock, as calculated from the combustion of HC and methane, respectively.

Energy and phosphorous recovery through hydrothermal carbonization of digested sewage sludge.

This work evaluates the potential of hydrothermal carbonization (HTC) to valorize the digestate derived from the anaerobic digestion of sewage sludge into useful materials for P and energy recovery. The hydrothermal treatment of digestate at 180-240 °C did not lead to high-rank hydrochars. On the other hand, inorganic P concentration did not change with the temperature, while as the carbonization temperature increased, the organic P retention yield in hydrochar became lower, increasing the total P in the process water obtained at the highest temperature, up to 25.3%. P recovery from acid leaching of the hydrochar obtained at 180 °C, via precipitation with CaO at pH up to 9, led to a brown solid precipitate with total P content close to 42 mg g-1, in the range of low grade phosphorus ores. Moreover, acid leaching reduced by 50% the ash content, yielding lignite-like upgraded hydrochars with higher heating values in the range of 20.5-23.1 MJ kg-1, fairly interesting as solid fuels. Anaerobic digestion of the process water enabled additional energy recovery in form of biogas (325 and 279 mL CH4 g-1 VS -at standard temperature and pressure; STP- from the process water resulting at 180 and 210 °C, respectively).

Contamination of N-poor wastewater with emerging pollutants does not affect the performance of purple phototrophic bacteria and the subsequent resource recovery potential.

Propagation of emerging pollutants (EPs) in wastewater treatment plants has become a warning sign, especially for novel resource-recovery concepts. The fate of EPs on purple phototrophic bacteria (PPB)-based systems has not yet been determined. This work analyzes the performance of a photo-anaerobic membrane bioreactor treating a low-N wastewater contaminated with 25 EPs. The chemical oxygen demand (COD), N and P removal efficiencies were stable (76 ±â€¯8, 62 ±â€¯15 and 36 ±â€¯8 %, respectively) for EPs loading rate ranging from 50 to 200 ng L-1 d-1. The PPB community adapted to changes in both the EPs concentration and the organic loading rate (OLR) and maintained dominance with >85 % of total 16S gene copies. Indeed, an increment of the OLR caused an increase of the biomass growth and activity concomitantly with a higher EPs removal efficiency (30 ±â€¯13 vs 54 ±â€¯11 % removal for OLR of 307 ±â€¯4 and 590 ±â€¯8 mgCOD L-1 d-1, respectively). Biodegradation is the main mechanism of EPs removal due to low EPs accumulation on the biomass, the membrane or the reactor walls. Low EPs adsorption avoided biomass contamination, resulting in no effect on its biological methane potential. These results support the use of PPB technologies for resource recovery with low EPs contamination of the products.

Toxicity and inhibition assessment of ionic liquids by activated sludge.

Toxicity of 13 ionic liquids (ILs) corresponding to different families were studied by inhibition respiration assays (15 min) using activated sludge. Toxicity increased as increasing the number of carbons in the alkyl-chain of imidazolium-based ILs, with EC50 values from 4.19 to 0.17 for 1-ethyl-3-methylimidazolium chloride ([Emim][Cl]) and 1-octyl-3-methylimidazolium chloride ([Omim][Cl]), respectively. An increase in toxicity was observed for aromatic-based ILs (pyridinium- and imidazolium-based ILs) due to the hydrophobic character of the head groups in comparison with linear structures as phosphonium and ammonium cations. Among to the anions studied fixing [Emim]+ as cation, [HSO4]- and [NTf2]- presented low EC50 values (0.34 mM and 1.69 mM, respectively) while [Cl]- and [EtSO4]- were considered harmless anions due to the hydrophilic character of chloride and the organic nature of [EtSO4]-. ILs toxicity/inhibition was determined by adding a biodegradable compound and measuring the sludge response after being in contact with the ILs for at least 15 h. The exposure of sewage sludge to ILs for more than 15 min used in short inhibition assays caused more toxic effect on microorganisms, even for [Choline][NTf2], previously defined as practically harmless (EC50 = 2.79 mM). Biodegradability assays confirmed the biodegradable nature of choline cation, related with TOC conversion of 40%, only due to cation consumption. No oxygen consumption or even lysis of microbial cells was observed for Tetrabutylammonium bis(trifluoromethylsulfonyl)imide and for 1-Ethyl-3-methylimidazolium hydrogensulphate due to the presence of anions previously defined as hazardous ([NTf2]- and [HSO4]-), maintaining their recalcitrant character to sewage systems.

Cation and anion effect on the biodegradability and toxicity of imidazolium- and choline-based ionic liquids.

This work studies the effect of the cation and anion on the biodegradability and inhibition of imidazolium- and choline-based ionic liquids (ILs) using activated sludge. Six commercial ILs, formed by combination of 1-Butyl-3-methylimidazolium (Bmim+) and N,N,N-trimethylethanolammonium (Choline+) cations and chloride (Cl-), acetate (Ac-) and bis(trifluoromethanesulfonyl)imide (NTf2-) anions were evaluated, all representative counter-ions with markedly different toxicity and biodegradability. Inherent and fast biodegradability tests were used to evaluate both the microorganism inhibition and the IL biodegradability. In addition, the ecotoxicological response (EC50) of the ILs was studied using activated sludge and Vibrio fischeri (Microtox® test). Bmim+ and NTf2- can be considered as non-biodegradable, whereas aerobic microorganisms easily degraded Choline+ and Ac-. The biodegradation pattern of each cation/anion is nearly unaffected by counter-ion nature. Moreover, concentrations of CholineNTf2 higher than 50 mg/L caused a partial inhibition on microbial activity, in good concordance with its low EC50 (54 mg/L) measured by respiration inhibition test, which alerts on the negative environmental impact of NTf2-containing ILs on the performance of sewage treatment plants.

Valorization of microalgal biomass by hydrothermal carbonization and anaerobic digestion.

The potential of hydrothermal carbonization (HTC) as a novel choice for treating microalgal biomass (MAB) was assessed. The hydrochar obtained at 210 °C had a carbon content and a higher heating value (HHV) 1.09 and 1.1 times greater, respectively, than that of the feedstock. Also, washing the hydrochar with HCl efficiently removed ash and increased its carbon content 1.40-fold. Energy recovery in the liquid fraction from the hydrothermal treatment (LF) by anaerobic digestion (AD) allowed methane yields of 188-356 mL STP CH4 g-1 VSadded, to be obtained. As a result, the amount of energy recovered from MAB was increased from about 4 MJ kg-1 (20% in terms of HHV) to 15.4, 12.1 and 10.4 MJ kg-1 by combining HTC at 180, 210 and 240 °C, respectively, with AD. Therefore, HTC at 180 °C in combination with AD seemingly provides an effective method for valorizing MAB.

Assessment the ecotoxicity and inhibition of imidazolium ionic liquids by respiration inhibition assays.

The ecotoxicity and inhibition of 12 imidazolium ionic liquids (ILs) with alkyl chain from C4 to C10 and chloride (Cl-), tetrafluoroborate (BF4-) and bis(trifluoromethanesulfonyl)imide (NTf2-) anions have been studied by means of respiration inhibition assays using activated sludge collected from a wastewater treatment plant. This test represents an alternative easy, economic and quick way to evaluate the true impact of ILs on activated sludge-based wastewater treatment. For comparison purposes, the EC50 values were also determined by the Microtox test (Vibrio fischeri). It was observed that this widely used microbial test overestimates the effect of the ILs on biological wastewater treatment facilities, especially in the case of ILs with lower ecotoxicity. The results of the biological tests showed that the alkyl chain length plays a crucial role in the ecotoxicity of ILs. A significant increase of the toxicity with the length of the n-alkyl chain was found. Regarding to the impact of the anion, the ecotoxicity measured by respiration inhibition assays follows the order NTf2- > Cl- > BF4-, being the anion effect higher as decreasing the length of cation alkyl chain. According to the hazard substances ranking for aquatic organisms (Passino and Smith, 1987), imidazolium ILs with C4 alkyl chain can be classified as "practically harmless" compounds whereas those with alkyl chains C8 or C10 correspond to "highly toxic" species.

Mesophilic anaerobic co-digestion of the organic fraction of municipal solid waste with the liquid fraction from hydrothermal carbonization of sewage sludge.

In the present study, the influence of substrate pre-treatment (grinding and sieving) on batch anaerobic digestion of the organic fraction of municipal solid waste (OFMSW) was first assessed, then followed by co-digestion experiments with the liquid fraction from hydrothermal carbonization (LFHTC) of dewatered sewage sludge (DSS). The methane yield of batch anaerobic digestion after grinding and sieving (20 mm diameter) the OFMSW was considerably higher (453 mL CH4 STP g-1 VSadded) than that of untreated OFMSW (285 mL CH4 STP g-1 VSadded). The modified Gompertz model adequately predicted process performance. The maximum methane production rate, Rm, for ground and sieved OFMSW was 2.4 times higher than that of untreated OFMSW. The anaerobic co-digestion of different mixtures of OFMSW and LFHTC of DSS did not increase the methane yield above that of the anaerobic digestion of OFMSW alone, and no synergistic effects were observed. However, the co-digestion of both wastes at a ratio of 75% OFMSW-25% LFHTC provides a practical waste management option. The experimental results were adequately fitted to a first-order kinetic model showing a kinetic constant virtually independent of the percentage of LFHTC (0.52-0.56 d-1) and decreasing slightly for 100% LFHTC (0.44 d-1).

Enhanced anaerobic degradability of highly polluted pesticides-bearing wastewater under thermophilic conditions.

This work presents a sustainable and cost-competitive solution for hardly biodegradable pesticides-bearing wastewater treatment in an anaerobic expanded granular sludge bed (EGSB) reactor at mesophilic (35°C) and thermophilic (55°C). The reactor was operated in continuous mode during 160days, achieving an average COD removal of 33 and 44% under mesophilic and thermophilic conditions, respectively. The increase of temperature improved the biomass activity and the production of methane by 35%. Around 96% of pesticides identified in raw wastewater were not detected in both mesophilic and thermophilic effluents. A dramatic selection of the microbial population in anaerobic granules was caused by the presence of pesticides, which also changed significantly when the temperature was increased. Pesticides caused a significant inhibition on methanogenesis, especially over acetoclastic methanogens. Aerobic biodegradability tests of the resulting anaerobic effluents revealed that aerobic post-treatment is also a feasible and effective option, yielding more than 60% COD reduction.

Fouling control in membrane bioreactors with sewage-sludge based adsorbents.

The potential application of powdered activated carbon (PAC) to mitigate membrane fouling has been tested in membrane bioreactors (MBRs) fed with cosmetic wastewater. Inexpensive powder activated carbon was prepared from sewage sludge biosolids (B-PAC) by pyrolysis (750 °C; 0.5 h) and air-activation (400 °C; 2 h). Adsorption capacities of 143 and 570 mg g-1 were reached for carbohydrates and proteins, respectively, quite similar to those of a commercial activated carbon (C-PAC). To check the effect of PAC addition on membrane fouling, three MBRs were simultaneously operated without (control-MBR) and with PAC (B-MBR and C-MBR) for 150 days in continuous mode at 8 L m-2 h-1 flux. Similar COD removal efficiencies were achieved in these three MBR systems. After 100 days of operation, the effect of the PACs on the sludge filterability was studied in the MBRs for 10 days. B-MBR showed stable transmembrane pressure (TMP) after 9 days of operation, unlike of control-MBR and C-MBR, where the TMP increased after the 2nd and 5th days, respectively. Therefore, operational cost saving can be achieved in the membrane cleaning due to decrease of fouling rate. Operating at stable state condition the addition of PAC gave rise to an increase of the critical flux of 25%. In an extra shear test, carried out at the end of the continuous experiment, a clear reduction in mean size of the flocs from 45 to 28 µm was observed in control-MBR. However, the extra shear led to a slight reduction of the mean size of flocs (less than 5%) in MBRs with PAC, with average sizes of 62 and 71 µm in C-MBR and B-MBR, respectively. The molecular weight fractionation of the MBR demonstrated a higher selectivity of B-PAC toward the adsorption of proteins smaller than 1 µm which prevents the irreversible fouling of the membranes. The membranes lifetime was increased because the B-PAC extended the filtration for a longer period than C-PAC, probably due to its easier in-situ regeneration.

Comparison of Fenton and Fenton-like oxidation for the treatment of cosmetic wastewater.

The treatment of cosmetic wastewaters by Fenton (Fe²âº/H2O2) and Fenton-like (Fe³âº/H2O2) oxidation has been studied. From batch and continuous experiments it has been proved that both versions of the Fenton process lead to quite similar results in terms of chemical oxygen demand (COD) and total organic carbon reduction although the COD shows a slightly higher rate in the early stages of reaction. COD reductions of around 55% after 2 h reaction time and 75-80% with 4 h residence time were reached in batch and continuous experiments, respectively, conducted at pH around 3, ambient temperature (20 °C), with 200 mg/L of Fe dose and an initial H2O2/COD weight ratio corresponding to the theoretical stoichiometric value. Achieving the locally allowable limit of COD for industrial wastewater discharge into the municipal sewer system takes no more than 30 min reaction time under those conditions by both Fenton systems. However, the Fenton-like process, where iron is fed as Fe(3+), would be preferable for industrial applications since the ferric sludge resulting upon final neutralization of the effluent can be recycled to the process. A second-order kinetic equation with respect to COD fitted fairly well the experimental results at different temperatures, thus providing a simple practical tool for design purposes.

Anaerobic biodegradability of mixtures of pesticides in an expanded granular sludge bed reactor.

The biodegradability and toxicity of three commercial pesticides containing 2-methyl-4-chlorophenoxyacetic acid (MCPA), imidacloprid and dimethoate were evaluated individually, and a complex mixture of these pesticides was treated in an expanded granular sludge bed (EGSB) reactor. MCPA was partially biodegraded, while imidacloprid and dimethoate remained almost unaltered during the individual biodegradability tests. Cyclohexanone was identified as the major solvent in the dimethoate-bearing insecticide, which was completely removed regardless of the presence of other pesticides. The analysis of the inhibition over the acetoclastic methanogenesis showed IC(50) (half maximal inhibitory concentration) values of 474 and 367 mg/L for imidacloprid and dimethoate, respectively. The effect on the methanogenesis was negligible in the case of MCPA and cyclohexanone. Pesticides caused a dramatic decrease of the EGSB reactor performance. After 30 d acclimation, the EGSB reactor achieved a stable chemical oxygen demand (COD) removal efficiency and methane production of around 85% and 0.9 g CH(4)-COD/g COD, respectively, for MCPA, imidacloprid, dimethoate and cyclohexanone feed concentrations of 57, 20, 25 and 27 mg/L, respectively. The presence of complex pesticide mixtures led to synergistic/antagonistic responses, reducing the MCPA biodegradation and improving the removal of the insecticides' active ingredients, which were completely removed in the EGSB reactor.

Coupling Fenton and biological oxidation for the removal of nitrochlorinated herbicides from water.

The combination of Fenton and biological oxidation for the removal of the nitrochlorinated herbicides alachlor, atrazine and diuron in aqueous solution has been studied. The H2O2 dose was varied from 20 to 100% of the stoichiometric amount related to the initial chemical oxygen demand (COD). The effluents from Fenton oxidation were analyzed for ecotoxicity, biodegradability, total organic carbon (TOC), COD and intermediate byproducts. The chemical step resulted in a significant improvement of the biodegradability in spite of its negligible or even slightly negative effect on the ecotoxicity. Working at 60% of the stoichiometric H2O2 dose allowed obtaining highly biodegradable effluents in the cases of alachlor and atrazine. That dose was even lower (40% of the stoichiometric) for diuron. The subsequent biological treatment was carried out in a sequencing batch reactor (SBR) and the combined Fenton-biological treatment allowed up to around 80% of COD reduction.

Strategies to evaluate biodegradability: application to chlorinated herbicides.

The biodegradability of nitrochlorinated (diuron and atrazine) and chlorophenoxy herbicides (2,4-D and MCPA) has been studied through several bioassays using different testing times and biomass/substrate ratios. A fast biodegradability test using unacclimated activated sludge yielded no biodegradation of the herbicides in 24 h. The inherent biodegradability test gave degradation percentages of around 20-30% for the nitrochlorinated herbicides and almost complete removal of the chlorophenoxy compounds. Long-term biodegradability assays were performed using sequencing batch reactor (SBR) and sequencing batch membrane bioreactor (SB-MBR). Fixed concentrations of each herbicide below the corresponding EC50 value for activated sludge were used (30 mg L(-1) for diuron and atrazine and 50 mg L(-1) for 2,4-D and MCPA). No signs of herbicide degradation appeared before 35 days in the case of diuron and atrazine and 21 days for 2,4-D, whereas MCPA was partially degraded since the early stages. Around 25-36% degradation of the nitrochlorinated herbicides and 53-77% of the chlorophenoxy ones was achieved after 180 and 135 days, respectively, in SBR, whereas complete disappearance of 2,4-D was reached after 80 days in SB-MBR.

Low-temperature anaerobic treatment of low-strength pentachlorophenol-bearing wastewater.

The anaerobic treatment of low-strength wastewater bearing pentachlorophenol (PCP) at psychro-mesophilic temperatures has been investigated in an expanded granular sludge bed reactor. Using an upward flow rate of 4 m h(-1), a complete removal of PCP, as well as COD removal and methanization efficiencies higher than 75% and 50%, respectively, were achieved. Methanogenesis and COD consumption were slightly affected by changes in loading rate, temperature (17-28°C) and inlet concentrations of urea and oils. Pentachlorophenol caused an irreversible inhibitory effect over both acetoclastic and hydrogenotrophic methanogens, being the later more resistant to the toxic effect of pentachlorophenol. An auto-inhibition phenomenon was observed at PCP concentrations higher than 10 mg L(-1), which was accurately predicted by a Haldane-like model. The inhibitory effect of PCP over the COD consumption and methane production was modelled by modified pseudo-Monod and Roediger models, respectively.