RESUMEN
This study evaluated the effects of linseed oil (LO) and two-grain sources on growth performance, microbial protein yield (MPY), blood metabolites, and inflammatory markers in Holstein dairy calves. Forty-eight 3-day-old dairy calves (24 males and 24 females) with starting BW of 40.3 ± 1.6 kg were allocated in a completely randomised block design with a 2 × 2 factorial arrangement as follows; (1) Corn grain (CG) with no LO supplementation (CG-NLO), (2) CG with 2.5% LO supplementation (CG-LO), (3) Barley grain (BG) with no LO supplementation (BG-NLO), and (4) BG with 2.5% LO supplementation (BG-LO). The calves were weaned on d 59 but the study lasted for 14 days after weaning (Day 73 of experiment). The results showed that starter feed intake was influenced neither by grain source nor linseed oil. However, average daily gain, BW, hip height, and MPY were improved in calves received BG compared to CG diets. Linseed oil supplementation had no significant effects on growth performance and MPY. During preweaning period, calves fed BG-LO had the greatest feed efficiency and the highest wither height. However, the greatest tumour necrosis factor and serum amyloid A were observed in BG-NLO. Despite, LO supplementation did not influence growth performance of animals per se; however, it reduced circulating inflammatory markers in calves during preweaning period. Based on this study condition, BG is more favourable than CG in dairy calves from the daily gain and microbial protein synthesis perspectives, and supplementing the starters with n-3 FA can be strategy to improve immune performance of calves fed barley-based starter diets.