Do people know how others view them? Two approaches for identifying the accuracy of metaperceptions.

Self-knowledge includes not only beliefs about one's own traits and abilities, but beliefs about how others view the self. Are such metaperceptions accurate? This article identifies two distinct standards used to determine meta-accuracy. The correlational approach tests whether metaperceptions correlate with an accuracy criterion (i.e. social perceptions). The mean-level approach instead asks whether metaperceptions tend to err in a systematic direction. This article reviews complementary lessons gleaned from research taking one approach or the other: whether metaperceptions merely reflect self-perceptions, whose metaperceptions are more or less accurate, and what psychological processes impede meta-accuracy, among others. Ultimately, neither approach is endorsed as unconditionally superior. Instead, which approach offers the proper accuracy standard should depend on the decisions those metaperceptions will guide.

The overblown implications effect.

People frequently engage in behaviors that put their competencies on display. However, do such actors understand how others view them in light of these performances? Eight studies support an overblown implications effect (OIE): Actors overestimate how much observers think an actor's one-off success or failure offers clear insight about a relevant competency (Study 1). Furthermore, actors overblow performances' implications even in prospect, before there are experienced successes or failures on which to ruminate (Studies 2 and 3). To explain the OIE, we introduce the construct of working trait definitions-accessible beliefs about what specific skills define a general trait or competency. When actors try to adopt observers' perspective, the narrow performance domain seems disproportionately important in defining the general trait (Study 4). By manipulating actors' working trait definitions to include other (unobserved) trait-relevant behaviors, we eliminated the OIE (Study 5). The final 3 studies (Studies 6a-6c) more precisely localized the error. Although actors and observers agreed on what a single success or failure (e.g., the quality of a single batch of cookies) could reveal about actors' narrow competence (e.g., skill at baking cookies), actors erred in thinking observers would feel this performance would reveal a considerable amount about the more general skill (e.g., cooking ability) and related specific competencies (e.g., skill at making omelets). Discussion centers on how the present theoretical account differs from previous explanations why metaperceptions err and identifies important open questions for future research. (PsycINFO Database Record (c) 2020 APA, all rights reserved).

Overestimating the valuations and preferences of others.

People often make judgments about their own and others' valuations and preferences. Across 12 studies (N = 17,594), we find a robust bias in these judgments such that people overestimate the valuations and preferences of others. This overestimation arises because, when making predictions about others, people rely on their intuitive core representation of the experience (e.g., is the experience generally positive?) in lieu of a more complex representation that might also include countervailing aspects (e.g., is any of the experience negative?). We first demonstrate that the overestimation bias is pervasive for a wide range of positive (Studies 1-5) and negative experiences (Study 6). Furthermore, the bias is not merely an artifact of how preferences are measured (Study 7). Consistent with judgments based on core representations, the bias significantly reduces when the core representation is uniformly positive (Studies 8A-8B). Such judgments lead to a paradox in how people see others trade off between valuation and utility (Studies 9A-9B). Specifically, relative to themselves, people believe that an identically paying other will get more enjoyment from the same experience, but paradoxically, that an identically enjoying other will pay more for the same experience. Finally, consistent with a core representation explanation, explicitly prompting people to consider the entire distribution of others' preferences significantly reduced or eliminated the bias (Study 10). These findings suggest that social judgments of others' preferences are not only largely biased, but they also ignore how others make trade-offs between evaluative metrics. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

Identification of residues within the African swine fever virus DP71L protein required for dephosphorylation of translation initiation factor eIF2α and inhibiting activation of pro-apoptotic CHOP.

The African swine fever virus DP71L protein recruits protein phosphatase 1 (PP1) to dephosphorylate the translation initiation factor 2α (eIF2α) and avoid shut-off of global protein synthesis and downstream activation of the pro-apoptotic factor CHOP. Residues V16 and F18A were critical for binding of DP71L to PP1. Mutation of this PP1 binding motif or deletion of residues between 52 and 66 reduced the ability of DP71L to cause dephosphorylation of eIF2α and inhibit CHOP induction. The residues LSAVL, between 57 and 61, were also required. PP1 was co-precipitated with wild type DP71L and the mutant lacking residues 52- 66 or the LSAVL motif, but not with the PP1 binding motif mutant. The residues in the LSAVL motif play a critical role in DP71L function but do not interfere with binding to PP1. Instead we propose these residues are important for DP71L binding to eIF2α.

Regulation of transcription of the Aedes albopictus cecropin A1 gene: A role for p38 mitogen-activated protein kinase.

Regulation of the Aedes albopictus cecropin A1 promoter was studied to provide insight into the transcriptional control of this antimicrobial peptide (AMP) gene in mosquitoes. Gene expression levels of cecropin A1 increased in A. albopictus C6/36 cells in response to heat-killed Escherichiacoli. Reporter gene assays incorporating -757 to +32 of the A. albopictus cecropin A1 promoter revealed that E. coli could induce expression in these cells with more pronounced expression than that seen with lipopolysaccharide (LPS). Analysis of deletion constructs demonstrated that the 5' boundary of the regulatory region for the activation of this AMP was located between -173 and -64. Western blotting with anti-phospho-specific antibodies demonstrated that p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinase (JNK) were activated by LPS, whereas only p38 MAPK was activated by E. coli. Moreover, pharmacological experiments revealed that pre-incubation of cells with the p38 MAPK inhibitor SB203580 resulted in a striking activation of the cecropin A1 promoter following immune challenge, demonstrating that p38 MAPK negatively regulates cecropin A1 promoter activity. Finally the region required for the negative regulation by p38 MAPK was identified as being between -173 and -64. This report is the first to show involvement of the p38 MAPK pathway in the negative regulation of AMP production in a mosquito.

The African swine fever virus DP71L protein recruits the protein phosphatase 1 catalytic subunit to dephosphorylate eIF2alpha and inhibits CHOP induction but is dispensable for these activities during virus infection.

The African swine fever virus (ASFV) DP71L protein is present in all isolates as either a short form of 70 to 72 amino acids or a long form of about 184 amino acids, and both of these share sequence similarity to the C-terminal domain of the herpes simplex virus ICP34.5 protein and cellular protein GADD34. In the present study we expressed DP71L in different mammalian cells and demonstrated that DP71L causes dephosphorylation of eukaryotic translation initiation factor 2 alpha (eIF2α) in resting cells and during chemical-induced endoplasmic reticulum stress and acts to enhance expression of cotransfected reporter genes. We showed that DP71L binds to all the three isoforms (α, ß, and γ) of the protein phosphatase 1 catalytic subunit (PP1c) and acts by recruiting PP1c to eIF2α. We also showed that DP71L inhibits the induction of ATF4 and its downstream target, CHOP. We investigated the eIF2α phosphorylation status and induction of CHOP in porcine macrophages infected by two ASFV field isolates, Malawi Lil20/1 and Benin 97/1, and two DP71L deletion mutants, MalawiΔNL and E70ΔNL. Our results showed that deletion of the DP71L gene did not cause an increase in the level of eIF2α phosphorylation or induction of CHOP, indicating that DP71L is not the only factor required by the virus to control the phosphorylation level of eIF2α during infection. We therefore hypothesize that ASFV has other mechanisms to prevent the eIF2α phosphorylation and the subsequent protein synthesis inhibition.

Cordycepin inhibits protein synthesis and cell adhesion through effects on signal transduction.

3'-Deoxyadenosine, also known as cordycepin, is a known polyadenylation inhibitor with a large spectrum of biological activities, including anti-proliferative, pro-apoptotic and anti-inflammatory effects. In this study we confirm that cordycepin reduces the length of poly(A) tails, with some mRNAs being much more sensitive than others. The low doses of cordycepin that cause poly(A) changes also reduce the proliferation of NIH3T3 fibroblasts. At higher doses of the drug we observed inhibition of cell attachment and a reduction of focal adhesions. Furthermore, we observed a strong inhibition of total protein synthesis that correlates with an inhibition of mammalian target of rapamycin (mTOR) signaling, as observed by reductions in Akt kinase and 4E-binding protein (4EBP) phosphorylation. In 4EBP knock-out cells, the effect of cordycepin on translation is strongly reduced, confirming the role of this modification. In addition, the AMP-activated kinase (AMPK) was shown to be activated. Inhibition of AMPK prevented translation repression by cordycepin and abolished 4EBP1 dephosphorylation, indicating that the effect of cordycepin on mTOR signaling and protein synthesis is mediated by AMPK activation. We conclude that many of the reported biological effects of cordycepin are likely to be due to its effects on mTOR and AMPK signaling.