RESUMEN
Imaging Raman spectroscopy can be used to identify cancerous tissue. Traditionally, a step-by-step scanning of the sample is applied to generate a Raman image, which, however, is too slow for routine examination of patients. By transferring the technique of integral field spectroscopy (IFS) from astronomy to Raman imaging, it becomes possible to record entire Raman images quickly within a single exposure, without the need for a tedious scanning procedure. An IFS-based Raman imaging setup is presented, which is capable of measuring skin ex vivo or in vivo. It is demonstrated how Raman images of healthy and cancerous skin biopsies were recorded and analyzed.
Asunto(s)
Interpretación de Imagen Asistida por Computador/métodos , Nevo/diagnóstico por imagen , Neoplasias Cutáneas/diagnóstico por imagen , Piel/diagnóstico por imagen , Espectrometría Raman/métodos , Anciano , Anciano de 80 o más Años , Algoritmos , Animales , Estudios de Casos y Controles , Diseño de Equipo , Antebrazo/diagnóstico por imagen , Humanos , Nevo/química , Fantasmas de Imagen , Procesamiento de Señales Asistido por Computador , Piel/química , Neoplasias Cutáneas/química , Espectrometría Raman/instrumentación , PorcinosRESUMEN
Until now, spatially resolved Raman Spectroscopy has required to scan a sample under investigation in a time-consuming step-by-step procedure. Here, we present a technique that allows the capture of an entire Raman image with only one single exposure. The Raman scattering arising from the sample was collected with a fiber-coupled high-performance astronomy spectrograph. The probe head consisting of an array of 20 × 20 multimode fibers was linked to the camera port of a microscope. To demonstrate the high potential of this new concept, Raman images of reference samples were recorded. Entire chemical maps were received without the need for a scanning procedure.
