Function of Brønsted and Lewis acid sites in xylose conversion into furfural.

In this work, the xylose conversion and the selectivity to furfural were assessed over mesoporous sulfonic silica SBA-15-(X)SO3H catalysts doped with metal ions (X = Al(iii), Ti(iv) or Zr(iv)). The type and amount of acid sites were analyzed by adsorption of pivalonitrile. The SBA-15-(X)SO3H materials show Lewis acid sites (LAS) and two types of Brønsted acid sites (BAS) with different strengths. Type I (BAS I) belongs to terminal silanol groups, type II (BAS II) is ascribed to hydroxyl groups bonded to sulfur or transition metal, and the LAS is related to M-O bonds. Optimal reaction conditions for the most active catalyst (SBA-15-(Zr)SO3H) were 120 minutes of reaction at 160 °C, 20 wt% of catalyst, and 2.5% of xylose/solvent. Additionally, a kinetic study was carried out to calculate the rate constants, the activation energy, and the pre-exponential factor for the xylose dehydration reaction. It was found that the selectivity to furfural in sulfonic silica SBA-15-(X)SO3H catalysts was directly related to the BAS II fraction. While LAS negatively impacts the selectivity to furfural leading to the undesired reaction between furfural and xylose obtaining humins as secondary products.

Comparison of dilute acid pretreatment of agave assisted by microwave versus ultrasound to enhance enzymatic hydrolysis.

A comparison between microwave and ultrasound irradiations in the agave pretreatment using dilute sulfuric acid as catalyst was assessed for the first time. Pretreatments were performed using a Taguchi Orthogonal Array L9 (34) to improve the hemicellulose removal and the agave digestibility. The results showed that under optimal conditions, the hemicellulose removal was superior in the pretreatment assisted with microwave (77.5%) compared to ultrasound (28.2%). Enzymatic hydrolysis yield of agave pretreated with microwave (MWOC) was 2-fold higher than agave pretreated with ultrasound (USOC). The relatively mild conditions of pretreatment with MWOC allowed to obtain a hydrolyzed free of inhibitors with a high glucose concentration (47.7 g/L) at low solids loading (10% w/v). However, these conditions did not have a significant effect over the agave pretreated with ultrasound. The pretreatment assisted with MWOC allowed to reduce time and temperature of the process compared to pretreatment with conventional heating.

Clostridium strain selection for co-culture with Bacillus subtilis for butanol production from agave hydrolysates.

In this work, three Clostridium strains were tested for butanol production from Agave lechuguilla hydrolysates to select one for co-culturing. The agave hydrolysates medium was supplemented with nutrients and reducing agents to promote anaerobiosis. Clostridium acetobutylicum ATCC 824 had the highest butanol production (6.04 g/L) and was selected for further analyses. In the co-culture process, Bacillus subtilis CDBB 555 was used to deplete oxygen and achieve anaerobic conditions required for butanol production. The co-culture was prepared with C. acetobutylicum and B. subtilis without anaerobic pretreatment. Butanol production in co-culture from agave hydrolysates was compared with experiments using synthetic medium with glucose and a pure culture of C. acetobutylicum. The maximum butanol concentration obtained was 8.28 g/L in the co-cultured hydrolysate medium. Results obtained in the present work demonstrated that agave hydrolysates have the potential for butanol production using a co-culture of B. subtilis and C. acetobutylicum without anaerobic pretreatment.

Autohydrolysis pretreatment assessment in ethanol production from agave bagasse.

The aim of the present work was to assess the autohydrolysis pretreatment of Agave tequilana bagasse for ethanol production. The pretreatment was conducted using a one-liter high pressure Parr reactor under different severity factors (SF) at a 1:6w/v ratio (solid:liquid) and 200rpm. The solids obtained under the selected autohydrolysis conditions were subjected to enzymatic hydrolysis with a commercial cellulase cocktail, and the enzymatic hydrolysate was fermented using Saccharomyces cerevisiae. The results obtained from the pretreatment process showed that the glucan content in the pretreated solid was mostly preserved, and an increase in the digestibility was observed for the case with a SF of 4.13 (190°C, 30min). Enzymatic hydrolysis of the pretreated solids showed a yield of 74.3%, with a glucose concentration of 126g/L, resulting in 65.26g/L of ethanol after 10h of fermentation, which represent a 98.4% conversion according to the theoretical ethanol yield value.