RESUMEN
Well-defined poly(methyl methacrylate) (PMMA) and poly(methyl acrylate) (PMA) are prepared via photochemically induced atom transfer radical polymerization (photoATRP) using ppm amounts of CuBr2 /tris(2-pyridylmethyl)amine and CuBr2 /tris[2-(dimethylamino)ethyl]amine catalyst complexes, respectively, without degassing of polymerization mixture and with no need to introduce an external reducing agent to the system. The effect of ligand to CuBr2 ratio on kinetic and induction period during the polymerization of MMA and MA is investigated. The induction period is influenced also by the amount of oxygen in the polymerization system. Both the kinetics of MA polymerization and the induction period are affected by light intensity. Finally, the high livingness and initiation efficiency of the photoATRP system in the presence of air are proved by chain extension polymerizations. The presented system is valuable from an industrial point of view, since after optimization, well-defined and high-molar-mass poly(meth)acrylates can be prepared without the necessity of degassing the system, while the polymerization can be proceeded quickly and without an induction period.
Asunto(s)
Metacrilatos/síntesis química , Oxígeno/química , Fotoquímica , Polimerizacion , Catálisis , Cinética , Metacrilatos/efectos de la radiaciónRESUMEN
Comprehensive characterization of nanoparticles associated with investigation of their cellular uptake creates the basis on which fundamental in vitro and in vivo studies can be built. In this work, a complex analysis of various surface-modified magnetite nanoparticles in biologically relevant environment is reported and the promotion of incorrect characterization into the results obtained from model biological experiments leading to false conclusions is demonstrated. Via a bottom-up approach from particle characterization by DLS towards interpretation of biological data based on cellular uptake, this work draws attention to the systematic propagation of errors stemming from inaccurate determination of input parameters for DLS, improper selection of particle size distribution, inadequate sampling, unknown colloidal behavior and the omission of fraction of particles complying with the internalization threshold. In addition, cellular uptake depending on the number of treated cells is shown. The definition of cellular uptake efficacy reflecting the size distribution of particles beside their absolute internalization is postulated.
Asunto(s)
Fenómenos Químicos , Espacio Intracelular/metabolismo , Nanopartículas de Magnetita/química , Células A549 , Coloides/química , Dispersión Dinámica de Luz , Endocitosis , Humanos , Hidrodinámica , Nanopartículas de Magnetita/ultraestructura , Tamaño de la Partícula , SolucionesRESUMEN
A highly compartmentalized enzymatic network regulates the pro-apoptotic and proliferative effects of sphingolipids. Over-conversion of ceramide (Cer) correlates with insensitivity to apoptosis signaling (in response to chemotherapy) and to drug resistance of cancer cells. De novo sphingomyelin biosynthesis relies on non-vesicular ceramide trafficking by the CERT (CERamide Transfer) protein. Therefore, blocking CERT transfer, thus leading to increased intracellular ceramide availability, represents a potential anticancer strategy. Our study is based on the implementation of an in vitro binding assay, supported by in silico molecular docking. It constitutes the first attempt to explore at the molecular level for the identification of novel CERT ligands. This approach is the first step toward in silico design and optimization of CERT inhibitor candidates, potentially relevant as innovative ceramide-transfer-targeting therapeutic agents.
Asunto(s)
Proteínas Portadoras/antagonistas & inhibidores , Proteínas Portadoras/metabolismo , Ceramidas/metabolismo , Transporte Biológico/efectos de los fármacos , Ligandos , Modelos Moleculares , Conformación MolecularRESUMEN
The practical stereodivergent route to both syn- and anti-diastereomers of 1-substituted 3-aminobutane-1,4-diols based on the crystallization-induced asymmetric transformation (CIAT) approach was completed. This led to the revision of the reported stereochemistry of the first inhibitor of CERT-dependent ceramide trafficking HPA-12 from (R,R)-anti- to the (R,S)-syn-enantiomer. Due to the expeditiousness of production and inexpensive conditions developed, a series of alkyl- and aryl-substituted analogues of HPA-12 is also reported.