CYP21A2 Gene Analysis in Southern Iranian CAH Patients and a Brief Review of the Mutation Spectrum.

Background: CYP21A2 gene mutations are responsible for more than 95% of Congenital Adrenal Hyperplasia (CAH) disorders with autosomal recessive inheritance. Most of these pathogenic mutations originate from the CYP21A1P, a neighboring pseudogene with 98% homology, due to unequal crossing over or gene conversion events. Mutation identification of the gene could be beneficial for accurate diagnosis and outcome prediction. Methods: Twelve unrelated patients with CAH diagnosis were recruited for genetic counseling. To ensure distinct amplification of the CYP21A2 gene rather than its pseudogene, the complete sequence of the gene was amplified through two overlapping fragments by specific primers. The entire sequences were screened by direct Sanger sequencing using new sequencing primers. Results: Only two pathogenic point mutations were identified. The c.293-13C>G, also known as In2G, and the c.955C>T mutations were found in 37.5 and 33.3% of alleles, respectively. One patient showed homozygous gene deletion. We also reviewed recent reports on CYP21A2 gene mutations in Iran. Conclusion: Evaluating the ethnicity-specific gene mutation data is significant for populations with diverse ethnic groups including the Iranian population. Although several common mutations have been reported as causative mutations among CAH patients, identifying only two common point mutations in Fars province would help prioritize exon sequencing and reduce the cost and time of genotyping.

Evaluation of the safety and efficacy of biosimilar recombinant growth hormone in children with growth hormone deficiency: non-inferiority, randomized, parallel, multicentric and Phase III trial.

OBJECTIVES: This study is designed in order to compare the efficacy and safety of recombinant human growth hormone (rhGH) with the reference brand. METHODS: According to the inclusion criteria, 85 people in 13 Iranian centers were randomly selected to receive biosimilar Somatropin (Somatin®) (44 people) and reference Somatropin (Norditropin®) (41 people) at a dose of 35 µg/kg/d, seven days/week for 12 months. The primary outcomes included height velocity (HV) was measured during 12 months of treatment. RESULTS: The two intervention groups' Height changes were similar. The mean HV was 10.96 cm/year in the biosimilar group and 10.05 cm/year in the reference groups after 12 months. Estimates of the lower bounds of 95% CI for mean height differences in the biosimilar intervention group compared to the reference intervention group did not exceed the 2 cm margin. Therefore, the non-inferiority of biosimilar intervention compared to the brand product is verified. Common ADRs in both groups were nausea in two patients (2.4%), diarrhea in two patients (2.4%), increased body temperature in one patient (1.2%), and headache in one patient (1.2%). CONCLUSIONS: The finding of this study indicated that Somatin® and Norditropin® have comparable efficacy and safety profiles. CLINICAL TRIAL REGISTRATION: www.IRCT.irIRCT20171122037571N1.

The effect of yoga therapy directed by virtual training on depression of adolescent girls with type 1 diabetes: a randomized controlled trial.

Background: Recently, the depression-alleviating impact of yoga therapy was documented among patients with type 2 diabetes; nonetheless, whether this consequence is similar in individuals with type 1 diabetes (T1D) is still unclear. Therefore, this trial sought to investigate the potential impact of yoga therapy on the depression of adolescents with T1D. Methods: This randomized controlled trial recruited 62 girls with T1D, aged 12-17 years, from January to June 2020. The participants were randomly allocated to equal experimental and control arms (31 per group) through a block randomization approach. The routine care was implemented in two study arms, while the experimental arm additionally received yoga therapy directed by virtual training for eight consecutive weeks (one session per week). Maria Kovacs Children's Depression Inventory was completed at baseline and the end of the 8-week intervention. Results: The mean of the depression total score was significantly lower in the experimental arm in comparison with the control arm at the trial end (9.38 ± 8.44 vs. 12.77 ± 6.96, p = 0.014). Also, the reduction in mean change from the baseline to the trial end was significantly more in the experimental arm (- 5.25 ± 1.13 vs. - 0.80 ± 1.00, p = 0.013). Conclusions: The administration of yoga therapy directed by virtual training seems to be potentially effective in reducing depression among adolescent girls with T1D. However, further long-term trials with a larger sample size are needed to shed light on the obtained findings and address the intervention's efficacy on glycemic outcomes. Supplementary Information: The online version contains supplementary material available at 10.1007/s40200-023-01245-x.

Determination of prediction equations for apparent metabolizable energy corrected for nitrogen of corn gluten meal and canola meal in broilers.

The objectives of this experiment were to determine the AMEn content of different samples of corn gluten meal (CGM) and canola meal (CM) by a reference diet method and to develop prediction equations based on the chemical composition to estimate the AMEn value of CGM and CM in broilers. A total of 300 one-day-old male broiler chicks were randomly assigned to fifteen treatments (14 experimental diet and 1 reference diet) with 4 replicates of each with 5 birds per replicate. At first, birds were fed a starter diet from 0 to 10 d of age, and then, a grower diet from 11 to 23 d of age. To determine the AMEn content, the test diet consisted of 60% reference diet, 38% each test CGM or CM, and 2% minor ingredients. To adaptation, the broilers were fed experimental diets for 4 d, and then feces were collected on 28 d. The gross energy values and chemical compositions among the CGM and CM from different origins were significantly different. The AMEn values of the CGM samples varied from 3,123 to 3,918 kcal/kg, and for the CM, the range was from 1,578 to 2,109 kcal/kg. At the end of the experiment, data were analyzed with SPSS software, and a regression equation was obtained based on the chemical composition. The best equations were selected based on the standard of prediction and regression adjusted R2. The equation, AMEn = 49.196 × CP + 80.87 × EE (SEP 180.99; adjusted R2 0.97), was selected to predict the AMEn value of CGM, and the equation, AMEn = 631.55 × EE + 16.716 × CP (SEP 55.3; adjusted R2 0.94), was selected to predict the AMEn value of CM.

Inborn Errors of Metabolism Associated With Autism Among Children: A Multicenter Study from Iran.

OBJECTIVE: This study aimed to find the common inborn errors of metabolism in Iranian patients with autism spectrum disorder. METHODS: In this cross-sectional multicenter study, 105 children and adolescents with autism spectrum disorder from six centers in different cities of Iran were enrolled between August, 2019 and October, 2020. Metabolic screening, including measuring plasma levels of amino acids, acylcarnitines, creatine, and guani-dinoacetate, and urinary levels of organic acids, purines, and pyrimidines was performed. Other data, including age, parental consanguinity, history of seizure, developmental mile-stones, and physical examination, were also recorded. RESULTS: An inborn error of metabolism was found in 13 (12.4%) patients. Five patients (4.8%) had cerebral creatine deficiency syndrome, 4 (3.8%) had arginine succinate aciduria, 2- methylbutyryl glycinuria, short-chain acyl-CoA dehydrogenase deficiency, and combined methylmalonic aciduria/malonic aciduria. There was a strong association between positive meta-bolic evaluation and parental consanguinity, history of seizures, microcephaly, and delayed development. CONCLUSIONS: Our results suggest that metabolic screening should be performed in the cases of autism associated with parental consanguinity, developmental delay, and a history of seizures. The assays to be considered as a screening panel include plasma or blood amino acids, acylcarnitines, creatine and guanidinoacetate, and urinary levels of organic acids.

Effects of Different Levels of Dietary Zinc-Threonine and Zinc Oxide on the Zinc Bioavailability, Biological Characteristics and Performance of Honey Bees (Apis mellifera L.).

The experiment was conducted to investigate the effect of supplementary different levels of zinc-threonine (Zn-Thr) and zinc oxide (ZnO) on the Zn bioavailability, biological characteristics and performance of honey bees (Apis mellifera L.). The experiments were carried out with seven treatments in a completely randomized design with five replicates for each treatment. During the experiment, groups were fed a basal diet without extra zinc (10.4 mg Zn/kg diet), and it was used as the control diet and 3 levels of 20, 40, and 60 mg Zn/kg were added to the diet by ZnO and Zn-Thr sources. The results showed that different levels of organic Zn significantly increased Zn and Fe content in the carcass of caged bees compared to different levels of inorganic Zn and control groups. Also, honey bees fed with levels of 40 and 60 mg Zn/kg Zn-Thr supplementation significantly had lower Malondialdehyde (MDA) concentration and higher ash content, protein content, superoxide dismutase (SOD) activity in their tissues. In addition, they showed more life span, feed intake, population, brood rearing, and hive weight gain (p < 0.05). Totally, the results of the present experiments revealed that diets supplied with organic Zn compared to inorganic Zn play significant roles in the improvement of Zn bioavailability, biological characteristics, and performance in honey bees.

Evaluation of an Innovative Zn Source on Feed Efficiency, Growth Performance, Skin and Bone Quality of Broilers Suffering Heat Stress.

One thousand two hundred male broilers were used to evaluate the effect of different dosages of HiZox® on feed efficiency, growth performance and bone quality of broilers suffering from heat stress. A completely randomized design was used, with four treatments and ten replicates. Basal corn−soybean meal diets supplemented with 75, 100 and 125 mg/kg zinc from HiZox and 100 mg/kg zinc from regular ZnO were used to make four treatments. Heat stress was induced after the third week by keeping house temperature between 28−34 °C, from 1 pm until 5 pm. The body weights of the birds that received the diet supplemented with HiZox or ZnO showed no significant difference at 7 and 14 days. Body weight of heat stressed birds fed diets containing different levels of HiZox or ZnO were not different at 28 and 42 days of age. In comparison to the Ross 308 management guide, induced heat stress diminished body weight and feed intake by approximately 17 and 21%, respectively. At 28 days, chickens who received 125 mg/kg Zn from Hizox had better feed efficiency (p < 0.05). The mortality rate of heat-stressed male broiler chickens who received different dosages of HiZox was 2.85% less than that of the regular ZnO group (p < 0.06). The results showed that addition of HiZox to the diet of male broiler under heat stress doubled the skin resistance during feather plucking in the slaughter plant and improved carcass quality (p < 0.07). Tibia breaking strength, included elongation and extension were improved by consumption of a diet supplemented with 75 mg HiZox/kg (p < 0.09). The HiZox-75 fed broilers required higher amounts of energy (MJ) for tibia breaking at break and peak points at 42 days (p < 0.09; p < 0.07). Jejunum Zn concentrations reflected the quantity of ingested Zn (p < 0.0001). Gizzard Zn solubility was dependent on dietary treatment (p < 0.03). Solubility of Zn in the gizzard of chickens who received HiZox was higher (about 30%) than broilers fed regular ZnO. In conclusion, Zn from HiZox was more efficient in decreasing heat stress mortality, increasing skin resistance and bone breaking strength compared to a regular ZnO source.

The frequency of CD4+ and CD8+ circulating T stem cell memory in type 1 diabetes.

INTRODUCTION: The frequencies and functions of T stem cell memory (TSCM) subsets vary in autoimmune diseases. We evaluated the frequencies of CD4+ and CD8+ TSCM subsets as well as their PD-1 expression levels in patients with T1D. METHODS: Blood samples were collected from new case (NC) (n = 15), and long-term (LT) (n = 15) groups and healthy controls (n = 15). Five subsets of T cells including TCM(CD4+ /CD8+ CCR7+ CD45RO+ CD95+ ), TCMhi (CD4+ /CD8+ CCR7+ CD45ROhi CD95+ ), TEM(CD4+ /CD8+ CCR7- CD45RO+ CD95+ ), TSCM(CD4+ /CD8+ CCR7+ CD45RO- CD95+ ), and T naive (CD4+ /CD8+ CCR7+ CD45RO- CD95- ) were detected by flow-cytometry. RESULTS: The frequency of CD4+ TSCM was higher in NC patients than LT patients and controls (p < .0001 and p = .0086, respectively). A higher percentage of the CD8+ T naive cells was shown in NC patients as compared with LT and healthy individuals (p = .0003 and p = .0002, respectively). An increased level of PD-1 expression was observed on the CD4+ TCM and TCMhi cells in LT patients as compared with healthy controls (p = .0037 and p = .0145, respectively). Also, the higher PD-1 expression was observed on the CD8+ TCM and TCMhi in NC and LT patients as compared with controls (p = .0068 and p < .0001; p = .0012 and p = .0012, respectively). CONCLUSION: Considering TSCMs' capacities to generate all memory and effector T cells, our results may suggest a potential association between the increased frequencies of TSCMs and T1D progression.

Carbulin: A Comprehensive Mobile Application for Advanced Carbohydrate Counting and Diet- and Insulin-Regimen Planning for Type 1 Diabetic Patients.

Nowadays, the introduction of the so-called 'diabetes technology', either hardware/device or software, to different aspects of day-to-day living in patients with diabetes aims to improve blood glucose control and various lifestyle features. The coordination of vast context of diabetes education/training, particularly in the area of medical nutrition therapy, is considered as a great concern. On the other hand, Iranian food culture consists of a set of traditional dietary patterns and food consumption habit. The study was aimed to develop "the Comprehensive Mobile Application of Advanced Carbohydrate Counting and Diet- and Insulin-Regimen Planning" to help type 1 diabetic patients, improving their health status. The programming language of Kotlin, JavaScript, Node JS, and HTML5 was used for the mobile app development. The app was developed with the following abilities: 1) educating users on different aspects of disease control including, updated general treatment guidelines on physical activity, medical nutrition and insulin therapy, stress management, and the patient's specific goals and dietary needs, 2) performing advanced carbohydrate counting using both picture-represented and kitchen-scale of carbohydrate foods as well as traditional Iranian foods, 3) recommending the patient's specific insulin dose, either short- or rapid-acting, based on the carbohydrate content of the selected meal or the selected amount of Iranian foods, 4) recommending the personalized insulin dose needed for decreasing the high blood glucose levels, and 5) performing 3 and 4 simultaneously. Developing Carbulin was an effort to increase type 1 diabetes self-management using the traditional Iranian dietary pattern and menu.

Molecular characterization of a large cohort of mucopolysaccharidosis patients: Iran Mucopolysaccharidosis RE-diagnosis study (IMPRESsion).

Mucopolysaccharidoses (MPSs) are rare, heterogeneous inborn errors of metabolism (IEM) diagnosed through a combination of clinical, biochemical, and genetic investigations. The aim of this study was molecular characterization of the largest cohort of Iranian MPS patients (302 patients from 289 unrelated families), along with tracking their ethnicity and geographical origins. 185/289 patients were studied using an IEM-targeted NGS panel followed by complementary Sanger sequencing, which led to the diagnosis of 154 MPS patients and 5 non-MPS IEMs (diagnostic yield: 85.9%). Furthermore, 106/289 patients who were referred with positive findings went through reanalysis and confirmatory tests which confirmed MPS diagnosis in 104. Among the total of 258 MPS patients, 225 were homozygous, 90 harbored novel variants, and 9 had copy number variations. MPS IV was the most common type (34.8%) followed by MPS I (22.7%) and MPS VI (22.5%). Geographical origin analysis unveiled a pattern of distribution for frequent variants in ARSB (c.430G>A, c.962T>C [p.Leu321Pro], c.281C>A [p.Ser94*]), GALNS (c.319G>A [p.Ala107Thr], c.860C>T [p.Ser287Leu], c.1042A>G [p.Thr348Ala]), and IDUA (c.1A>C [p.Met1Leu], c.1598C>G [p.Pro533Arg], c.1562_1563insC [p.Gly522Argfs*50]). Our extensive patient cohort reveals the genetic and geographic landscape of MPS in Iran, which provides insight into genetic epidemiology of MPS and can facilitate a more cost-effective, time-efficient diagnostic approach based on the region-specific variants.

Uncovering Cardiac Involvement in Childhood Diabetes: Is it Time to Move Toward Speckle Tracking Echocardiography in Childhood Diabetes Management?

CONTEXT: One of the most common endocrine disorders in children is diabetes which is the leading cause of premature cardiovascular disease in adulthood. AIMS: This study is aimed to investigate the extend of cardiac involvement in diabetic children by speckle tracking echocardiography (STE) in comparison to two-dimensional (2D) echocardiography and routine laboratory data. SETTINGS AND DESIGN: A cross-sectional study conducted on patients under 18 years of age who deal with type one diabetes mellitus for more than 5 years. SUBJECTS AND METHODS: To compare the STE results, we included the STE data of 25 normal age-matched children. All patients underwent laboratory analysis for lipid profile, blood sugar, and 2D echocardiography plus STE. STATISTICAL ANALYSIS USED: Two-sample independent t-test, Chi-square test, logistic regression test, Spearman's rank correlation coefficient, and the Pearson correlation coefficient. RESULTS: From March 2018 to 2019, we included 53 patients, mean age 15.8 ± 0.39 years and 52.8% female, and 25 nondiabetic control in this study. STE revealed global longitudinal strain (LS) -18.4 versus - 24.2 for patient (44 valid cases) versus control group, respectively, with significant statistical difference. Diabetic patients had lower LS in all segments compared to the control group. CONCLUSIONS: STE has very high sensitivity to detect cardiac involvement far earlier than 2D echocardiography. None of the routine biomarkers or demographic features can predict cardiac involvement based on segmental abnormalities of STE. Active investigation to clear the remote impact of STE abnormalities and its practical role in childhood diabetes management is highly recommended.

Recommendations for Infantile-Onset and Late-Onset Pompe Disease: An Iranian Consensus.

Background: Pompe disease, also denoted as acid maltase or acid α-glucosidase deficiency or glycogen storage disease type II, is a rare, autosomal recessive lysosomal storage disorder. Several reports have previously described Pompe disease in Iran and considering increased awareness of related subspecialties and physicians, the disease's diagnosis is growing. Objective: This guideline's main objective was to develop a national guideline for Pompe disease based on national and international evidence adapting with national necessities. Methods: A group of expert clinicians with particular interests and experience in diagnosing and managing Pompe disease participated in developing this guideline. This group included adult neurologists, pediatric neurologists, pulmonologists, endocrinologists, cardiologists, pathologists, and physiatrists. After developing search terms, four authors performed an extensive literature review, including Embase, PubMed, and Google Scholar, from 1932 to current publications before the main meeting. Before the main consensus session, each panel member prepared an initial draft according to pertinent data in diagnosis and management and was presented in the panel discussion. Primary algorithms for the diagnosis and management of patients were prepared in the panel discussion. The prepared consensus was finalized after agreement and concordance between the panel members. Conclusion: Herein, we attempted to develop a consensus based on Iran's local requirements. The authors hope that disseminating these consensuses will help healthcare professionals in Iran achieve the diagnosis, suitable treatment, and better follow-up of patients with infantile-onset Pompe disease and late-onset Pompe disease.

Role of Selenium Deficiency in Pediatrics with Acquired Hypothyroidism.

BACKGROUND: Although selenium is one of the nutrients that has an important role in the metabolism of thyroid hormones, it has been investigated in rare studies. This study aimed to evaluate role of selenium deficiency in children and adolescents with acquired hypothyroidism. METHODS: This case and control study was conducted on 60 acquired hypothyroidism and 60 healthy children who had been referred to the pediatrics endocrine clinic in Shiraz, Iran, from November 2018 to April 2019. Some information such as age, gender, weight, height, duration of disease, and level of plasma selenium were recorded. Plasma selenium level was measured by atomic absorption spectrophotometer. Data were analyzed using SPSS software 21.0. RESULTS: The mean of selenium level in the case and control groups were 93.77 ± 24.90 µg/dl and 85.96 ± 25.20 µg/dl, respectively. There was no significant difference between the two groups in the mean levels of selenium. Independent t-test showed no significant difference in the mean levels of selenium in male and female samples in the case group, but this difference was significant in the control group. CONCLUSION: Selenium deficiency may not have significant role as a cause of acquired hypothyroidism in pediatric and adolescent age group, in south of Iran. Thus, it does not seem necessary to routinely check the level of selenium for patients with thyroid dysfunction.

Optimal Frequency to Screen Celiac Disease amongst Patients with Type 1 Diabetes Mellitus: A Multicenter Study.

AIMS: Celiac disease (CD) is frequent amongst patients with type 1 diabetes mellitus (T1DM). Since there is a disagreement on the optimal interval and frequency to perform screening tests for CD among diabetic patients, this study aimed to evaluate these issues amongst patients with T1DM. METHODS: This retrospective cohort study was conducted in seven referral diabetic centers in different cities of Iran from January 2020 to January 2021. Data belonging to 106 patients who were affected by both T1DM and CD was collected. The time interval between CD diagnosis and diabetes (IBCD), the age of diabetes onset, and any associated diseases, symptoms, and family history of T1DM and CD were recorded and analyzed. RESULTS: Results show that 45% of the patients with CD were diagnosed during the first year of diabetes onset; furthermore, 18% and 16% of the patients with CD were diagnosed in the second or third year after being diagnosed with diabetes. In addition, another 18% of patients with CD were diagnosed during the fourth till the eighth year after diabetes onset. Moreover, there was a negative relationship between the age of T1DM diagnosis and IBCD. Most participants were asymptomatic at the time of CD diagnosis. CONCLUSIONS: Screening tests to detect CD amongst patients with T1DM should continue for at least eight years after the initial T1DM diagnosis, especially those affected at a younger age.

Comparison of Zinc Sulfate and Zinc Threonine Based on Zn Bioavailability and Performance of Broiler Chicks.

The purpose of this study was to compare zinc sulfate and zinc threonine chelate based on Zn bioavailability and performance of broiler chicks. The study was conducted in a completely randomized design with 256 day-old Ross 308 chicks and eight treatments including control treatment (no zinc supplementation), three levels of zinc sulfate and zinc threonine chelate (40, 80, and 120 mg zinc per kg feed), and a common commercial chelate (Bioplex Zn®) supply 40 mg zinc per kg feed. The results of total period showed that threonine chelate group had the highest live weight compared with other treatments and lowest feed conversion ratio belonged to 80 and 120 ppm of zinc threonine chelate (p < 0.05). Zinc threonine chelate and commercial chelate treatments had the lowest cholesterol and LDL levels compared with other treatments (p < 0.05). Zinc chelate threonine which contains 80 and 120 ppm of zinc had the highest HDL and superoxide dismutase enzymes and the lowest heterophile to lymphocyte ratio compared with other treatments (p < 0.05). Relative bioavailability of zinc threonine to zinc sulfate based on body weight, feed conversion ratio, cholesterol, LDL, HDL, superoxide dismutase enzyme, ash, and zinc content in tibia were 418.75, 173.91, 131.38, 159.43, 278.63, 193.45, 156.46, and 117.65%, respectively. According to the results of broiler performance and other traits measured in this study, it seems that the use of 80 ppm of zinc threonine chelate in the broiler diet is recommended in comparison with zinc sulfate levels and other threonine chelate levels.

Comparison the Zn-Threonine, Zn-Methionine, and Zn Oxide on Performance, Egg Quality, Zn Bioavailability, and Zn Content in Egg and Excreta of Laying Hens.

The experiment was conducted to investigate the effect of supplementary zinc-threonine (Zn-Thr), zinc-methionine (Zn-Met), and zinc oxide (ZnO) on the laying performance, egg quality, Zn content in egg and excreta. One hundred and sixty hens (Hy-Line W36) were randomly divided into 10 treatments with 4 replications of 4 hens each. During the first 4 weeks, groups were fed a corn-soy basal diet without extra zinc (30.3 mg Zn/kg diet) to depletion storage zinc from the body and it was used as the control diet. During the ensuing 10 weeks from 36 to 45 weeks of age, 3 levels of 30, 60, and 90 mg Zn/kg were added to the diet by ZnO, Zn-Met, and Zn-Thr sources. Results showed that there were no significant differences between the experimental treatments in terms of egg weight, feed intake, eggshell weight, eggshell percentage, and albumen weight. In total experimental period, the supplementation of Zn-Met and Zn-Thr decreased feed conversion ratio and increased egg mass and egg production compared with ZnO 30 and 60 mg Zn/kg and control treatments (P < 0.05). The results showed that treatment effect on Zn content in egg and excreta was significant (P < 0.05). It is concluded that diets supplied with lower levels of organic Zn can maintain egg production performance, improve egg quality, enrich eggs, increase bioavailability, and reduce the negative effects of age on egg quality characteristics in laying hens compared with their oxide form.

Growth retardation among children in southern Iran: a 7-year population based cohort study.

BACKGROUND: Growth retardation is a common health problem, which requires early prevention and detection. This study was conducted to define the approximate age at which stunting starts among the Iranian boys and girls. METHOD: The second phase of a population-based retrospective cohort nested case-control study on 400 children who were followed from birth to 7 years of age. This study was performed to define the pattern of growth among stunted and normal children and to reveal the age at which stunting starts in each gender. RESULTS: Of the selected participants, 53% were girls. Also, about 18% of the children registered by the selected health centers were defined as stunted (under the 3rd percentile of the corresponding sex-age NCHS/WHO growth reference). For boys, the height was relatively similar between the two groups until the age of 6 months at which the difference in height between normal and stunted children starts to become significantly large (difference = 0.70 cm, P = 0.04). For girls, height in the two groups is relatively similar until the age of 9 months at which the difference starts to become significantly large (difference = 0.97 cm, P = 0.01). No significant difference in the weight of the girls was observed between the normal and stunted groups during the study period (difference = 283.21 g, P > 0.05). However, boys from the stunted group were lighter since almost the same time that they started to become significantly shorter (difference = 1265.19 g, P = 0.001). CONCLUSIONS: Soon after birth (at about the 6 months of age), the growth pattern of some (stunted) children starts to stumble and divert from normal. The sixth month of age is the age at which mothers start weaning with withdrawing breast milk and start supplementary foods and adult diet. A specially designed study is needed to understand the actual reason for observing such a phenomenon among Iranian children.

Relationship between chemical composition and standardized ileal digestible amino acid contents of corn grain in broiler chickens.

This experiment was conducted to evaluate the chemical composition and standardized ileal amino acid digestibility of corn grain and to use these data to develop prediction equations for estimating total amino acids (TAAs) and standardized ileal digestible amino acids (SIDAAs) for broiler chickens. Four types of corn grains were obtained from different origins (Brazil, Ukraine, Russia, and Iran). Eighty-day-old Ross 308 male broiler chicks were fed a standard diet until day 18, and experimental diets were fed from 19 to 24 D of age. Five dietary treatments consisted of 4 semi-purified diets containing corn from each origin as the only source of amino acid (AA) and a N-free diet for determination of basal endogenous AA losses. Assay diets contained 939 g of test corn/kg. The concentration of crude protein and gross energy ranged from 7.58 to 8.39% (coefficient of variation [CV] = 4.72%) and 4,121 to 4,621 kcal/kg (CV = 5.09%), respectively. There was significant variation among the 4 corn grains in standardized ileal digestibility (SID) for CP, Phe, Leu, Asp, Glu, Ser, Gly, Ala, and Tyr (P ≤ 0.05). The results of linear regression showed that linear prediction equations based on protein content can be used to predict the TAA and SIDAA contents (e.g., TLys = 0.041 × CP, adj R2 = 95.9, standard error of prediction [SEP] = 0.05; SIDLys = 0.0356 × CP, adj R2 = 96, SEP = 0.051). Inclusion of other proximate components of test samples into the regression equation increased the R2 value and decreased the SEP value (e.g., TLys = 0.329 × crude fiber [CF] ‒ 0.209 × Ash, adj R2 = 99.9, SEP = 0.005; SIDLys = â€’1.1591 + 0.836 × CF ‒ 0.055 × Ash, adj R2 = 99.9, SEP = 0.001). The concentration of TAA and SIDAA was highly correlated (adj R2 > 89%) for most AA and showed that the amount of SIDAA could be predicted from its total concentration with a high degree of accuracy (e.g., SIDLys = 0.0023 + 0.861 × TLys, adj R2 = 99.9, SEP = 0.0001). In conclusion, this in vitro assays and equations accurately predicted TAA and SIDAA corn grain samples for broiler chickens and can serve as a reference analysis to develop calibration equations for rapid feed quality evaluation methods such as near-infrared reflectance spectroscopy.

Prediction of the total and standardized ileal digestible amino acid contents from the chemical composition of soybean meals of different origin in broilers.

The objective of this experiment was to determine total amino acid (TAA) content, standardized ileal digestibility (SID) of crude protein, and standardized ileal amino acid digestibility in 9 sources of soybean meal (SBM) of different origin and to subsequently establish equations for predicting the TAA content and concentration of standardized ileal digestible amino acids (SIDAA) based on their protein content and other proximate components. Concentration of SIDAA of the samples was also predicted using TAA values. A total of 160 1-day-old male broiler chicks were randomly assigned to 10 dietary treatments consisted of 9 semipurified diets containing one SBM (200 g of crude protein/kg) as the only source of dietary amino acid (AA) and one N-free diet to determine endogenous ileal AA flow. The birds were fed with a standard diet from 0 to 18 D of age, and experimental diets were fed from 19 to 24 D of age. The fitness of the models of the study was tested using the adjusted coefficient of determination (R2) value, P-value regression and coefficients, and standard error of prediction (SEP). The coefficient of SID for Lys and Cys among SBM varied from 86.7 to 96.3 and 74.1 to 89.3, respectively, with significant difference (P < 0.05). In equations based on protein content, the adjusted R2 value ranged from 40.7 (Ile) to 99.6 (Met) and 37.2 (Met + Cys) to 99.6 (Met) for TAA content and concentration of SIDAA, respectively. Inclusion of other proximate components of test samples (e.g., crude fiber, neutral detergent fiber, acid detergent fiber, ash, gross energy, and so on) into the regression equation increased the adjusted R2 value and decreased the SEP. The results of linear regression revealed that it is possible to satisfactorily estimate the TAA content and concentration of SIDAA of SBM through its protein content and other proximate components, but the prediction equations based on other proximate components were more accurate in terms of reflecting the measured results; however, additional time and costs were associated with this approach. It is also possible to estimate the concentration of SIDAA through TAA values with reasonable accuracy and lower SEP.