Developing quantitative analysis program of blood flow velocity according to vessel diameter for neovascular age-related macular degeneration using OCTA-VISTA.

This study aimed to develop a quantitative analysis program of blood flow velocity by vessel diameter in neovascular age-related macular degeneration (nAMD) subjects using high-speed swept-source optical coherence tomography angiography. This retrospective, observational, cross-sectional study included 10 eyes of healthy volunteers and 4 eyes of patients with representative nAMD. Novel scan patterns and variable interscan time analysis were utilized to measure the flow parameter, a surrogate marker of blood flow velocity, by vessel diameter within different depths. Detected vessels at superficial and deep as well as outer retinal regions were categorized into three vessel diameters (major vessels (> 40 µm), medium vessels (20-40 µm), and capillaries (< 20 µm)). The flow parameter increased with enlarged vessel diameter in all participants at superficial and deep layer. All nAMD subjects, except for type 3 macular neovascularization (MNV), contained a structure dominated by medium vessels at outer retinal region. The mean flow parameter at outer retinal region was type 1 MNV (1.46 ms-1), type 1 + 2 MNV (0.98 ms-1), and polypoidal choroidal vasculopathy, including branching vascular networks (1.46 ms-1). This program provides the possibility to extract the blood flow information at different depths by vessel diameter types, which is considered to be useful tool for evaluating nAMD pathology and activity.

Extended and adjustable field-of-view of variable interscan time analysis by ammonite-scanning swept-source optical coherence tomography angiography.

A novel scanning protocol, ammonite scan, is proposed for widefield optical coherence tomography angiography (OCTA) and relative retinal blood flow velocity imaging in the human retina using variable interscan time analysis (VISTA). A repeated circle scan using a 400 kHz swept-source was employed to achieve an interscan time of 1.28 ms. The center of the repeated circular scan continuously moved spirally towards the peripheral region, ensuring an extended and adjustable scan range while preserving the short interscan time. Image artifacts due to eye movement were eliminated via extra motion-correction processing using data redundancy. The relative blood flow velocity in superficial and deep plexus layers was calculated from the VISTA image, and their ratio was used to explore the microvascular flow parameter in the healthy human eye.

Sensorless astigmatism correction using a variable cross-cylinder for high lateral resolution optical coherence tomography in a human retina.

High lateral resolution (∼5µm) optical coherence tomography (OCT) that employs a variable cross-cylinder (VCC) to compensate for astigmatism is presented for visualizing minute structures of the human retina. The VCC and its sensorless optimization process enable ocular astigmatism correction of up to -5.0 diopter within a few seconds. VCC correction has been proven to increase the signal-to-noise ratio and lateral resolution using a model eye. This process is also validated using the human eye by visualizing the capillary network and human cone mosaic. The proposed method is applicable to existing OCT, making high lateral resolution OCT practical in clinical settings.

Injection-seeded terahertz-wave parametric generator with timing stabilized excitation for nondestructive testing applications.

An injection-seeded terahertz (THz)-wave parametric generator (is-TPG) with a footprint the size of an A3 paper is presented. We improved the measurement performance of the is-TPG source for nondestructive inspection applications. A high pulse repetition rate up to 70 kHz and a low pulse timing jitter of a few tens of picoseconds, which is approximately one ten-thousandth of the conventional is-TPG, were achieved. THz waves exhibited excellent performance with a maximum average output power of 20 µW, a monochromatic spectrum linewidth of ∼20 GHz, and a frequency tuning range of 1.7-3.0 THz. This was achieved by designing the entire system configuration from the pump laser source to THz-wave generation. A new double-pass all-solid-state optical amplifier was developed with a high gain and low noise using an externally pulse-modulated laser diode (LD) as the master oscillator source. An achromatic optical injection system was developed for the is-TPG with a 40% reduction in the conventional path length. They were housed in a single enclosure in two layers. LDs and optical fiber amplifiers could be rack-mounted, and the outputs were delivered to the housing via optical fibers. The developed THz-wave source performed nondestructive imaging of a human hair sample fixed with Scotch tape on a test pattern in an envelope by irradiating 2.1 THz waves. A clearly recognizable THz-wave image of an enclosed hair with a spatial resolution close to the THz wavelength was obtained.

Frequency-agile injection-seeded terahertz-wave parametric generation: publisher's note.

This publisher's note contains corrections to Opt. Lett.45, 77 (2020)OPLEDP0146-959210.1364/OL.45.000077.