RESUMEN
The current study determined if interleukin-6 (IL-6) had a causative role in the lung dysfunction and/or surfactant alterations associated with three different lung insults. IL-6 (or saline) was instilled into rats followed by mechanical ventilation in vivo for 4 hours. Also, IL-6 (-/-) and wild-type mice were subjected to 3 insults: ex vivo injurious mechanical ventilation; cecal ligation and perforation; and hyperoxia exposure. In all experiments, the presence or absence of IL-6 did not significantly influence gas exchange, lung compliance, or various surfactant measurements. These results suggest that IL-6 may have a limited role in the surfactant alterations observed in acute lung injury.
Asunto(s)
Interleucina-6/inmunología , Interleucina-6/farmacología , Pulmón/efectos de los fármacos , Surfactantes Pulmonares/metabolismo , Síndrome de Dificultad Respiratoria/inmunología , Animales , Lavado Broncoalveolar/efectos adversos , Ciego/lesiones , Modelos Animales de Enfermedad , Hiperoxia/complicaciones , Interleucina-6/genética , Pulmón/fisiología , Masculino , Ratones , Ratones Endogámicos , Ratones Transgénicos , Ratas , Ratas Sprague-Dawley , Respiración Artificial/efectos adversos , Síndrome de Dificultad Respiratoria/etiología , Sepsis/tratamiento farmacológico , Sepsis/inmunologíaRESUMEN
An imbalance in matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases (TIMPs) leads to excessive or insufficient tissue breakdown, which is associated with many disease processes. The TIMP-3 null mouse is a model of MMP/TIMP imbalance, which develops air space enlargement and decreased lung function. These mice responded differently to cecal ligation and perforation (CLP)-induced septic lung injury than wild-type controls. The current study addresses whether the TIMP-3 knockout lung is susceptible to different types of insults or only those involving sepsis, by examining its response to lipopolysaccharide (LPS)-induced sepsis, mechanical ventilation (MV), and hyperoxia. TIMP-3 null noninjured controls of each insult consistently demonstrated significantly higher compliance vs. wild-type mice. Null mice treated with LPS had a further significantly increased compliance compared with untreated controls. Conversely, MV and hyperoxia did not alter compliance in the null lung. MMP abundance and activity increased in response to LPS but were generally unaltered following MV or hyperoxia, correlating with compliance alterations. All three insults produced inflammatory cytokines; however, the response of the null vs. wild-type lung was dependent on the type of insult. Overall, this study demonstrated that 1) LPS-induced sepsis produced a similar response in null mice to CLP-induced sepsis, 2) the null lung responded differently to various insults, and 3) the null susceptibility to compliance changes correlated with increased MMPs. In conclusion, this study provides insight into the role of TIMP-3 in response to various lung insults, specifically its importance in regulating MMPs to maintain compliance during a sepsis.
Asunto(s)
Hiperoxia/metabolismo , Enfermedades Pulmonares/metabolismo , Respiración Artificial , Sepsis/metabolismo , Inhibidor Tisular de Metaloproteinasa-3/fisiología , Animales , Hiperoxia/inducido químicamente , Hiperoxia/patología , Lipopolisacáridos/farmacología , Rendimiento Pulmonar , Enfermedades Pulmonares/inducido químicamente , Enfermedades Pulmonares/patología , Metaloproteinasas de la Matriz/metabolismo , Ratones , Ratones Noqueados , Sepsis/inducido químicamente , Sepsis/patología , Inhibidor Tisular de Metaloproteinasa-3/genéticaRESUMEN
Matrix metalloproteinases (MMPs) are degradative enzymes, which act to remodel tissue. Their activity is regulated by the tissue inhibitors of metalloproteinases (TIMPs). An imbalance in the degradation/inhibition activities has been associated with many diseases, including sepsis. We have previously shown that TIMP-3 knockout animals develop spontaneous, progressive air space enlargement. The objectives of this study were to determine the effects of a septic lung stress induced by cecal ligation and perforation (CLP) on lung function, structure, pulmonary surfactant, and inflammation in TIMP-3 null mice. Knockout and wild-type animals were randomized to either sham or CLP surgery, allowed to recover for 6 h, and then euthanized. TIMP-3 null animals exposed to sham surgery had a significant increase in lung compliance when compared with sham wild-type mice. Additionally, the TIMP-3 knockout mice showed a significant increase in compliance following CLP. Rapid compliance changes were accompanied by significantly decreased collagen and fibronectin levels and increased gelatinase (MMP-2 and -9) abundance and activation. Additionally, in situ zymography showed increased airway-associated gelatinase activity in the knockout animals enhanced following CLP. In conclusion, exposing TIMP-3 null animals to sepsis rapidly enhances the phenotypic abnormalities of these mice, due to increased MMP activity induced by CLP.