Asunto(s)
Dermatitis/epidemiología , Leucemia Linfocítica Crónica de Células B/patología , Neoplasias Cutáneas/epidemiología , Piel/patología , Anciano , Anciano de 80 o más Años , Comorbilidad , Dermatitis/genética , Dermatitis/patología , Progresión de la Enfermedad , Femenino , Genes de las Cadenas Pesadas de las Inmunoglobulinas/genética , Humanos , Leucemia Linfocítica Crónica de Células B/epidemiología , Leucemia Linfocítica Crónica de Células B/genética , Masculino , Persona de Mediana Edad , Mutación , Estadificación de Neoplasias , Factores de Riesgo , Neoplasias Cutáneas/genética , Neoplasias Cutáneas/patología , Proteína p53 Supresora de Tumor/genéticaAsunto(s)
Linfoma de Burkitt/genética , Metilación de ADN , Herpesvirus Humano 4 , Secuenciación de Nucleótidos de Alto Rendimiento , Linfoma de Burkitt/virología , Línea Celular Tumoral , Exoma , Regulación Neoplásica de la Expresión Génica , Herpesvirus Humano 4/genética , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Humanos , Cariotipificación , Polimorfismo de Nucleótido SimpleRESUMEN
The recently discovered MLT/MALT1 gene is fused with the API2 gene in the t(11;18)(q21;q21), which characterizes about one-third of MALT lymphomas. In order to screen for variant translocations and amplifications of MLT/MALT1, we have developed a novel, undirected two-color interphase fluorescence in situ hybridization (FISH) assay with two PAC clones flanking MLT/MALT1. This assay was applied to 108 marginal zone B-cell lymphomas (MZBCLs), including 72 extranodal MALT lymphomas, 17 nodal, and 19 splenic MZBCL. In 19 MALT lymphomas (26%), but in none of the nodal or splenic MZBCL, separated hybridization signals of the MLT/MALT1 flanking probes, were found. Further FISH analyses showed that 12 of these 19 cases displayed the classical t(11;18) and the remaining seven cases revealed the novel t(14;18)(q32;q21), involving the MLT/MALT1 and IGH genes. The frequency at which these translocations occurred varied significantly with the primary location of disease. The t(11;18) was mainly detected in gastrointestinal MALT lymphomas, whereas the t(14;18) occurred in MALT lymphomas of the parotid gland and the conjunctiva. Amplification of MLT/MALT1 was not observed in any of the lymphomas analyzed. We conclude that the translocations t(11;18)(q21;q21) and t(14;18)(q21;q32) represent the main structural aberrations involving MLT/MALT1 in MALT lymphomas, whereas true amplifications of MLT/MALT1 occur rarely in MZBCL.
