Bioaccumulation and health risk assessment of trace elements in Tilapia (Oreochromis mossambicus) from selected inland water bodies.

The presence of toxic trace elements (TEs) has resulted in a worldwide deterioration in freshwater ecosystem quality. This study aimed to analyze the distribution of TEs, including chromium (Cr), nickel (Ni), arsenic (As), mercury (Hg), cadmium (Cd), and lead (Pb), in water, sediment, and organs of Tilapia (Oreochromis mossambicus) collected from selected inland water bodies in Tamil Nadu, India. The water samples exhibited a range of concentrations for TEs: Cr varied from 0.014 to 5.193 µg/L, Ni ranged from 0.283 to 11.133 µg/L, As ranged from 0.503 to 1.519 µg/L, Cd from 0.001 to 0.616 µg/L, and Pb ranged from non-detectable (ND) to 6.103 µg/L. The concentrations of TEs in sediment were found to vary within the following ranges: 5.259 to 32.621 mg/kg for Cr, 1.932 to 30.487 mg/kg for Ni, 0.129 to 0.563 mg/kg for As, 0.003 to 0.011 mg/kg for Cd, ND to 0.003 mg/kg for Hg, and 0.404 to 1.575 mg/kg for Pb. The study found that the accumulation pattern of TE in fishes across all selected areas was liver > bone > gill > muscle. The organs had TE concentrations of Cr (ND-0.769 mg/kg), Ni (ND-1.053 mg/kg), As (0.002-0.080 mg/kg), Pb (ND-0.411 mg/kg), and Hg (ND-0.067 mg/kg), which was below the maximum residual limit prescribed by EC and FSSAI. The bioconcentration factor (BCF) of TEs exhibited a greater magnitude in comparison with the biota-sediment accumulation factor due to the higher concentration of TEs in fish and lower level in water. The assessment of both carcinogenic and non-carcinogenic risks suggests that the consumption of Tilapia from the study region does not pose any significant risks.

Employing Piper longum extract for eco-friendly fabrication of PtPd alloy nanoclusters: advancing electrolytic performance of formic acid and methanol oxidation.

Advancement in bioinspired alloy nanomaterials has a crucial impact on fuel cell applications. Here, we report the synthesis of PtPd alloy nanoclusters via the hydrothermal method using Piper longum extract, representing a novel and environmentally friendly approach. Physicochemical characteristics of the synthesized nanoclusters were investigated using various instrumentation techniques, including X-ray photoelectron spectroscopy, X-ray diffraction, and High-Resolution Transmission electron microscopy. The electrocatalytic activity of the biogenic PtPd nanoclusters towards the oxidation of formic acid and methanol was evaluated chronoamperometry and cyclic voltammetry studies. The surface area of the electrocatalyst was determined to be 36.6 m2g-1 by Electrochemical Surface Area (ECSA) analysis. The biologically inspired PtPd alloy nanoclusters exhibited significantly higher electrocatalytic activity compared to commercial Pt/C, with specific current responses of 0.24 mA cm - 2 and 0.17 mA cm - 2 at synthesis temperatures of 180 °C and 200 °C, respectively, representing approximately four times higher oxidation current after 120 min. This innovative synthesis approach offers a promising pathway for the development of PtPd alloy nanoclusters with enhanced electrocatalytic activity, thereby advancing fuel cell technology towards a sustainable energy solution.

Polyurethane degradation by extracellular urethanase producing bacterial isolate Moraxella catarrhalis strain BMPPS3.

Plastic waste has become a global issue and a threat to the ecosystem. The present study isolated polyurethane (PU) degrading bacterial species from soil dumped with plastic wastes. Four bacterial isolates, RS1, RS6, RS9 and RS13 were obtained and their ability to degrade PU in a synthetic medium with PU as a sole source of carbon was assessed individually. After thirty days of incubation, the highest PU weight loss of 67.36 ± 0.32% was recorded in the medium containing RS13 isolate. The results of FTIR revealed the occurrence of carbonyl peaks. The putative isolate RS13 confirmed with the genus Moraxella according to 16S rRNA gene sequencing and the isolate was specified as Moraxella catarrhalis strain BMPPS3. The restriction analysis of Moraxella catarrhalis strain BMPPS3 revealed that the GCAT content to 51% and 49% correspondingly. Moraxella catarrhalis strain BMPPS3 was able to colonize on PU surface and form a biofilm as revealed by SEM investigation. Fatty acids and alkanes were found to be the degradation products by GC-MS analysis. The presence of these metabolites facilitated the growth of strain RS13 and suggested that ester hydrolysis products had been mineralized into CO2 and H2O. Extracellular biosurfactant synthesis has also been found in Moraxella catarrhalis strain BMPPS13 inoculated with synthetic media and mineral salt media containing PU and glucose as carbon sources, respectively with a significant level of cell-surface hydrophobicity (32%). The production and activity of extracellular esterase showed consistent increase from day 1-15 which peaked (1.029 mM/min/mg) on day 24 significantly at P < 0.001. Crude biosurfactants were lipopeptide-based, according to the characteristic investigation. According to this study findings, Moraxella catarrhalis produces biosurfactants of the esterase, urethanase and lipase (lipopeptide) types when carbon source PU is present.

Diffusion of organochlorine (OCPs) and cypermethrin pesticides from rohu (Labeo rohita) internal organs to edible tissues during ice storage: a threat to human health.

The migration of organochlorine pesticides (OCPs) and cypermethrin residues from internal organs to edible tissues of ice-held Labeo rohita (rohu) was investigated in this study. The liver (246 µg/kg) had the highest level of ∑OCP residues, followed by the gills (226 µg/kg), intestine (167 µg/kg), and muscle tissue (54 µg/kg). The predominant OCPs in the liver and gut were endosulfan (53-66 µg/kg), endrin (45-53 µg/kg), and dichloro-diphenyl-trichloroethane (DDT; 26-35 µg/kg). The ∑OCP residues in muscle increased to 152 µg/kg when the entire rohu was stored in ice, but they decreased to 129 µg/kg in gill tissues. On days 5 and 9, the total OCPs in the liver increased to 317 µg/kg and 933 µg/kg, respectively. Beyond day 5 of storage, total internal organ disintegration had led to an abnormal increase in OCP residues of liver-like mass. Despite a threefold increase in overall OCP residues by day 9, accumulation of benzene hexachloride (BHC) and heptachlor was sixfold, endrin and DDT were fourfold, aldrin was threefold, and endosulfan and cypermethrin were both twofold. Endosulfan, DDT, endrin, and heptachlor were similarly lost in the gills at a rate of 40%, while aldrin and BHC were also lost at 60 and 30%, respectively. The accumulation of OCP residues in tissues has been attributed to particular types of fatty acid derivatives. The study concluded that while pesticide diffusion to edible tissues can occur during ice storage, the levels observed were well below the allowable limit for endosulfan, endrin, and DDT.

Potentially toxic metals in seawater, sediment and seaweeds: bioaccumulation, ecological and human health risk assessment.

This study assesses the bioaccumulation, ecological, and health risks associated with potentially toxic metals (PTMs), including Pb, Hg, Cd, As, and Cr in Hare Island, Thoothukudi. The results revealed that the concentration of PTMs in sediment, seawater, and S. wightii ranged from 0.095 to 2.81 mg kg-1, 0.017 to 1.515 mg L-1, and 0.076 to 5.713 mg kg-1, respectively. The highest concentrations of PTMs were found in the S. wightii compared to seawater and sediment. The high bioaccumulation of Hg and As in S. wightii suggests that it can be used as a bioindicator for these elements in this region. The ecological risk indices, which include individual, complex, biological, and ecological pollution indices, suggest that Hare Island had moderate contamination with Hg and Cd. However, there are no human health risks associated with PTMs. This study examines the current ecological and health risks associated with PTMs and emphasizes the importance of regular monitoring.

A response surface model to examine the reactive red 239 sorption behaviors on Rhizoclonium hieroglyphicum: isotherms, kinetics, thermodynamics and toxicity analyses.

The present study is an attempt to investigate the potentiality of Rhizoclonium hieroglyphicum in the removal of reactive red 239 (RR239) from aqueous solution and to assess the toxicity of the treated dye solution. Optimisation of the process variables namely dye and biosorbent concentrations, pH, temperature and incubation time for RR239 removal was performed using Response Surface Methodology (RSM) assisted Box Behnken Design (BBD) model. The recycling and regeneration efficiency of the dye adsorbed alga was evaluated using different eluents under optimized conditions. Further to understand the adsorption mechanism, isotherms, kinetics and thermodynamic studies were performed. UV-vis and FT-IR spectroscopy was employed to confirm the interaction between the adsorbate and biosorbent. The nature of the treated dye solution was assessed using phyto, microbial and brine shrimp toxicity studies. On the basis of quadratic polynomial equation and response surfaces given by RSM, 90% decolorization of RR239 was recorded at room temperature under specified optimal conditions (300 mg/L of dye, 500 mg/L of biosorbent, pH 8 and 72 h of contact time). Desorption experiments demonstrated 88% of RR239 recovery using 0.1 N acetic acid as an eluent and 81% of dye removal in regeneration studies. The data closely aligned with Freundlich isotherm (R2 - 0.98) and pseudo-second-order kinetic model (R2 - 0.9671). Thermodynamic analysis revealed that the process of adsorption was endothermic, spontaneous, and favorable. UV-Vis and FT-IR analyses provided evidence for adsorbate-biosorbent interaction, substantiating the process of decolorization. In addition, the results of phyto, microbial and brine shrimp toxicity assays consistently confirmed the non-toxic nature of the treated dye. Thus, the study demonstrated that R. hieroglyphicum can act as a potent bioremediation agent in alleviating the environmental repercussions of textile dyeing processes.

Synthesis of iron oxide nanoparticles using orange fruit peel extract for efficient remediation of dye pollutant in wastewater.

The removal of color-causing compounds from wastewater is a significant challenge that industries encounter due to their toxic, carcinogenic, and harmful properties. Despite the extensive research and development of various techniques with the objective of effectively degrading color pollutants, the challenge still persists. This paper introduces a simple technique for producing iron oxide nanoparticles (Fe2O3 NPs) using orange fruit peel for sustainable dye degradation in aqueous environment. The observation of color change and the measurement of UV-visible absorbance at 240 nm provided a confirmation for the development of Fe2O3 NPs. Transmission electron microscopy examination demonstrated that the Fe2O3 NPs have an agglomerated distribution and forming spherical structures with size ranging from 25-80 nm. Energy-dispersive X-ray spectroscopy analysis supported the existence of Fe and O. Fourier transform infrared spectroscopy conducted to investigate the involvement of orange peel extract in the reduction, capping, and synthesis of Fe2O3 NPs from the precursor salt. Fe2O3 NPs showed a photocatalytic remediation of 97%, for methylene blue under visible light irradiation. Additionally, prepared NPs exhibited concentration depended biofilm inhibition action against E. coli and S. aureus. In conclusion, Fe2O3 NPs can efficiently purify water and suppress pathogens due to their strong degrading activity, reusability, and biofilm inhibition property.

A review on simultaneous heavy metal removal and organo-contaminants degradation by potential microbes: Current findings and future outlook.

Industrial processes result in the production of heavy metals, dyes, pesticides, polyaromatic hydrocarbons (PAHs), pharmaceuticals, micropollutants, and PFAS (per- and polyfluorinated substances). Heavy metals are currently a significant problem in drinking water and other natural water bodies, including soil, which has an adverse impact on the environment as a whole. The heavy metal is highly poisonous, carcinogenic, mutagenic, and teratogenic to humans as well as other animals. Multiple polluted sites, including terrestrial and aquatic ecosystems, have been observed to co-occur with heavy metals and organo-pollutants. Pesticides and heavy metals can be degraded and removed concurrently from various metals and pesticide-contaminated matrixes due to microbial processes that include a variety of bacteria, both aerobic and anaerobic, as well as fungi. Numerous studies have examined the removal of heavy metals and organic-pollutants from different types of systems, but none of them have addressed the removal of these co-occurring heavy metals and organic pollutants and the use of microbes to do so. Therefore, the main focus of this review is on the recent developments in the concurrent microbial degradation of organo-pollutants and heavy metal removal. The limitations related to the simultaneous removal and degradation of heavy metals and organo-pollutant pollutants have also been taken into account.

New insights into defects and magnetic interactions inducing lattice disordering in Co2Fe0.5Cr0.5Al.

Atomic scale understanding of defect induced magnetic interactions resulting in lattice disordering has been deduced in a detailed manner for the first time in Co2Fe0.5Cr0.5Al based on Mössbauer spectroscopic studies and compared with the results obtained in Co2Fe0.8Cr0.2Al and Co2FeAl. An interesting linear correlation between valence electron concentration and the mean hyperfine fields at Fe sites in Co2FeAl based compounds has been deduced which is observed to exhibit different slopes with the substitution of Cr. This study elucidates an important role of the manifestation of the magnetic interactions especially between Fe, Co and Cr atoms leading to significant changes in the concentration and specific types of defects selectively produced in Co2Fe0.5Cr0.5Al as compared with that of Co2Fe0.8Cr0.2Al subjected to similar non-equilibrium treatments in this study. Further, for the first time this study elucidates the striking correlation of the effective value of the hyperfine field with the degree of ordering/disordering of the lattice with the Fe atoms associated with ordered sites experiencing a much higher value of the hyperfine field as compared to that of the disordered sites. This study also proposes optimal annealing treatment for the recovery of defects in Co2Fe0.5Cr0.5Al, which would be of significant importance in these spintronic materials.

Extraction and characterization of exopolysaccharides from Lactiplantibacillus plantarum strain PRK7 and PRK 11, and evaluation of their antioxidant, emulsion, and antibiofilm activities.

Exopolysaccharides (EPS) are produced by probiotic bacteria Lactiplantibacillus plantarum PRK7 and L. plantarum PRK11. The structure of EPS-7 and EPS-11 was characterized by Fourier-transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), gas chromatography-mass spectroscopy (GCMS), and thermogravimetric analysis (TGA). Further, in in vitro studies antioxidant, emulsion, and antibiofilm activity were investigated. The FTIR spectrum confirmed the presence of polysaccharides in EPS-7 and EPS-11, with absorbance at 1654.93 and 1655.33 cm-1, respectively. H1 NMR further confirmed the presence of glucose, galactose, xylose, and mannose. Sugar derivatives in EPS-7 and EPS-11 were further confirmed with GCMS. The SEM analysis revealed that EPS-7 had a weblike structure and EPS-11 had a smooth porous layer. The result of the TGA revealed that EPS-7 and EPS-11 had greater thermal stability at 319.1 and 300.1 °C, respectively. Furthermore, EPS-7 and EPS-11 showed a good percentage of free radical scavenging in DPPH (89.77 % and 93.1 %), ABTS (57.65 % and 58.63 %), hydroxyl radical scavenging (44.46 % and 40.308 %), and reducing power assay. The emulsion activity was confirmed with edible oils such as coconut oil, sesame oil, almond oil, castor oil, and neem oil. The highest emulsion activity for EPS-7 and EPS-11 was found with coconut and castor oil. In addition, the antibiofilm activity against pathogens revealed that EPS can prevent biofilm formation. Thus, it was found that EPS-7 and EPS-11 possess good structural characteristics and their biological activity makes them ideal for applications in the food and pharmaceutical industry.

Development of ZnO/SnO2/rGO hybrid nanocomposites for effective photocatalytic degradation of toxic dye pollutants from aquatic ecosystems.

The impact of ZnO/SnO2/reduced graphene oxide nanocomposites (ZnO/SnO2/rGO NCs) for improved photocatalytic degradation of organic dye pollution is examined in this study. The developed ternary nanocomposites had a variety of characteristics that were detected, such as crystallinity, recombination of photogenerated charge carriers, energy gap, and surface morphologies. When rGO was added to the mixture, the optical band gap energy of ZnO/SnO2 was lowered, which improved the photocatalytic activity. Additionally, in comparison to ZnO, ZnO/rGO, SnO2/rGO samples, the ZnO/SnO2/rGO nanocomposites demonstrated exceptional photocatalytic effectiveness for the destruction of orange II (99.8%) and reactive red 120 dye (97.02%), respectively after 120 min exposure to sunlight. The high electron transport properties of the rGO layers, which make it feasible to efficiently separate electron-hole pairs, are attributed to the enhanced photocatalytic activity of the ZnO/SnO2/rGO nanocomposites. According to the results, synthesized ZnO/SnO2/rGO nanocomposites are a cost-efficient option for removing dye pollutants from an aqueous ecosystem. Studies show that ZnO/SnO2/rGO nanocomposites are effective photocatalysts and may one day serve as the ideal material to reduce water pollution.

Current trends and prospects in catalytic upgrading of lignocellulosic biomass feedstock into ultrapure biofuels.

Eco-friendly renewable energy sources have recommended as fossil fuel alternatives in recent years to reduce environmental pollution and meet future energy demands in various sectors. As the largest source of renewable energy in the world, lignocellulosic biomass has received considerable interest from the scientific community to advance the fabrication of biofuels and ultrafine value-added chemicals. For example, biomass obtained from agricultural wastes could catalytically convert into furan derivatives. Among furan derivatives, 5-hydroxymethylfurfural (HMF) and 2, 5-dimethylfuran (DMF) are considered the most useful molecules that can be transformed into desirable products such as fuels and fine chemicals. Because of its exceptional properties, e.g., water insolubility and high boiling point, DMF has studied as the ideal fuel in recent decades. Interestingly, HMF, a feedstock upgraded from biomass sources can easily hydrogenate to produce DMF. In the present review, the current state of the art and studies on the transformation of HMF into DMF using noble metals, non-noble metals, bimetallic catalysts, and their composites have discussed elaborately. In addition, comprehensive insights into the operating reaction conditions and the influence of employed support over the hydrogenation process have demonstrated.

Synergistic role of metal oxide loading cocatalysts on photocatalytic degradation of organic pollutants and inactive bacteria over template-free ZnFe2O4 nanocubes.

For wastewater treatment, a highly reliable and ecologically friendly oxidation method is always preferred. This work described the production of a new extremely effective visible light-driven Ag2Ox loaded ZnFe2O4 nanocomposties photocatalyst using a wet impregnation technique. Under visible light irradiation, the produced Ag2Ox loaded ZnFe2O4 nanocomposties were used in the photodegradation of rhodamine B (RhB) and Reactive Red 120 (RR120) dyes. Analysis using X-ray diffraction, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and transmission electron microscopy revealed that Ag2Ox nanoparticles were well dispersed on the surface of ZnFe2O4 NPs and that the Ag2Ox loaded ZnFe2O4 NPs were created. When compared with bare ZnFe2O4 NPs, Ag2Ox-loaded ZnFe2O4 nanocomposites showed better photocatalytic activity for RhB and RR120 degradation under visible light (>420 nm) illumination. The reaction kinetics and degradation methodology, in addition to the photocatalytic degradation functions of Ag2Ox-loaded ZnFe2O4 nanocomposites, were thoroughly investigated. The 3 wt% Ag2Ox loaded ZnFe2O4 nanocomposites have a 99% removal efficiency for RhB and RR120, which is about 2.4 times greater than the ZnFe2O4 NPs and simple combination of 1 wt% and 2 wt% Ag2Ox loaded ZnFe2O4 nanocomposites. Furthermore, the 3 wt% Ag2Ox loaded ZnFe2O4 nanocomposites demonstrated consistent performance without decreasing activity throughout 3 consecutive cycles, indicating a potential approach for the photo-oxidative destruction of organic pollutants as well as outstanding antibacterial capabilities. According to the findings of the experiments, produced new nanoparticles are an environmentally friendly, cost-efficient option for removing dyes, and they were successful in suppressing the development of Gram-negative and Gram-positive bacteria.

Toxicity analysis and biomarker response of Quinalphos Organophosphate Insecticide (QOI) on eco-friendly exotic Eudrilus eugeniae earthworm.

An ever-increasing use of pesticides in agricultural fields has led to a catastrophic decline in crop quality and, ultimately soil fertility. To control various pests, quinalphos is commonly used in India's tea plantations. This study aims to investigate the effects of the Quinalphos organophosphate insecticide on the non-target beneficial organism Eudrilus eugeniae earthworms and the biomarkers that respond to its effects. Earthworm species, especially E. eugeniae, remains as the most trustworthy and well-suited model organism for conducting a wide variety of environmental studies. The median lethal concentration (LC50) was identified as 3.561 µg cm-2 (contact filter paper) and 1.054 mg kg-2 (artificial soil toxicity). The 5% and 10% of LC50 value 3.561 µg cm-2 was exposed to earthworm to analyze the sublethal effects at pre-clitellum, clitellum, and post-clitellum segments. Specific enzymatic activities of neurotransmitter enzyme acetylcholinesterase; antioxidant enzymes such as lipid peroxidase, superoxide dismutase, and catalase; and detoxification enzymes including glutathione S transferase, reduced glutathione, carboxylesterase, and Cytochrome P450 were analyzed. Exposure of E. eugeniae earthworm to subacute exposures of pesticides caused significant alterations in these stress markers in a concentration-dependent manner. Morphological abnormalities like bulginess, coiling, and bleeding were observed after exposure of the insecticide treatments. Histological cellular disintegration, a reduced NRRT time, and an inhibited proteolytic zone were also identified in pesticide-exposed earthworms. Studies demonstrate that the organophosphate insecticide quinalphos causes acute toxicity in E. eugeniae; hence, it is suggested that non-target eco-friendly E. eugeniae earthworms may be at risk if exposed to the excessive concentrations of quinalphos organophosphate insecticide in soil.

A mini-review on innovative strategies for simultaneous microbial bioremediation of toxic heavy metals and dyes from wastewater.

Bioremediation of heavy metals and dyes is one of the emerging techniques globally as it is evident from the numerous publications made by various research groups. Biofilm-assisted bioremediation is one of the trending approaches as it facilitates negatively charged extracellular polymeric substances which makes the bacteria resistant to the toxic chemicals. Genetic engineering of microbes will make them unique in the bioremediation process. This mini-review concentrates on source and toxic effects of heavy metals and dyes on aqueous and living beings. Further, the genetic improvement strategies for effective bioremediation are described. However, the gap between practicability and real-time applicability needs to test with real-time wastewater in the industrial scale.

Sodium hydroxide pre-treated Aspergillus flavus biomass for the removal of reactive black 5 and its toxicity evaluation.

The present study was focused on the removal of Reactive Black 5 (RB5) from aqueous solution using pre treated Aspergillus flavus as a biosorbent. Pre-treatment of fungal biomass with 0.1 M sodium hydroxide facilitated the removal of dye effectively when compared to untreated fungal biomass. Optimum biosorption conditions for RB5 removal was determined as a function of dye concentration (50-400 mg/L), biosorbent concentration (100-500 mg/L), incubation time (1-7hrs), pH (3-8) and temperature (20-50 °C). At the optimum conditions, the maximum removal efficiency of RB5 achieved by NaOH pretreated A. flavus was 91%. The dye removal was studied kinetically and it obeys the pseudo-second order model and the experimental equilibrium data well fitted the Langmuir isotherm indicating monolayer adsorption of dye molecules on the biosorbent. The thermodynamic parameters such as a change in free energy (ΔG), enthalpy (ΔH) and entropy (ΔS) were calculated and negative values of ΔG suggested that the dye removal process was spontaneous at all temperatures. Furthermore, the values of ΔH revealed that the adsorption process was endothermic. Recovery of RB5 from the fungal biomass was effective using 0.1 M Na2CO3 as an eluent. The interaction of adsorbate with biosorbent was analyzed using UV-Vis and FT-IR spectroscopy, SEM and XRD analyses. Phytotoxicity and microbial toxicity studies revealed the non-toxic nature of the treated dye solution. Hence, the fungal biomass pretreated with NaOH was efficient in decolorizing RB5 as well as composite raw industrial effluent generated from dyeing industries.

Facile fabrication of (2D/2D) MoS2@MIL-88(Fe) interface-driven catalyst for efficient degradation of organic pollutants under visible light irradiation.

The present investigation describes the photocatalytic degradation of methylene blue (MB) and rhodamine-B (RhB) using molybdenum disulfide (MoS2) anchored metal-organic frameworks (MOFs) under visible light irradiation. Herein, MIL-88(Fe) was successfully modified with MoS2 to yield a novel heterogeneous MoS2@MIL-88(Fe) hybrid composite. The prepared catalyst enhances the superior photocatalytic activity than the pristine form of MoS2 and MIL-88(Fe) framework. The physico-chemical properties of the prepared catalyst were analytically investigated and the results exhibit greater photocatalytic efficiency towards the chosen dyes, with an optical band gap of 2.75 eV. The MoS2 and MIL-88(Fe) framework could act as efficient oxidation and reduction sites in the as-synthesized MoS2@MIL-88(Fe) composite, and generated the non-toxic by-products such as hydroxyl (•OH), and superoxide species (•O2-) for the mineralization of MB and RhB dyes. The degradation kinetics showed that the dye system followed a pseudo-first-order model which is well supported by the Langmuir-Hinshelwood mechanism. Moreover, the reusability studies showed excellent photocatalytic activity after five cycles. Finally, the photocatalytic degradation mechanism of MB and RhB dyes was suggested.

Influence of biochar and EDTA on enhanced phytoremediation of lead contaminated soil by Brassica juncea.

Phytoremediation technology is an eco-friendly technology for the treatment of the polluted environment. Conversely, the natural and synthetic amendments have been revealed to improve the heavy metal phytoextraction from polluted soils with hyperaccumulation and/or non-hyper accumulating plants. This study evaluated the synergistic effect of biochar (BC) and EDTA to enhance phytoextraction of heavy metal lead (Pb) from artificially polluted soil by Brassica juncea. The BC and EDTA amendment enhanced the growth and survival of B. juncea under Pb stress environment. BC and EDTA significantly increased the biomass of B. juncea and significantly increased the total chlorophyll content in the combined amendment of BC and EDTA (22.2 mg/g) compared to the individual amendment of BC (12.8 mg/g) and EDTA (12.2 mg/g) respectively. The combined use of EDTA and biochar showed enhanced Pb uptake (60.2 mg/g) compared to control (10.0 mg/g). The order of Pb uptake was found to be BC + EDTA (60.2 mg/g) ˃ EDTA (23.5 mg/g) ˃ BC (22. 0 mg/g) ˃ control (10.0 mg/g). The maximum activity of SOD (35.2 ± 1.2 U/mg), POD (47.0 ± 1.8 U/mg) and CAT (28.0 ± 1.0 U/mg) was obtained in the mixed application of EDTA and BC. The obtained results revealed that the combined use of BC and EDTA was the most advantageous option for the treatment of Pb contaminated soil as compared to individual amendments.

Production and purification of laccase by Bacillus sp. using millet husks and its pesticide degradation application.

Lignocellulosic agricultural bi-products, pearl millet (PM) and finger millet (FM) husks, were used for the production of laccase using Bacillus sp. PS under solid-state fermentation (SSF). Abiotic variables such as substrate (PM, FM) concentration (1-5%), incubation time (24-96 h) and pH (5-10) were optimized using Response surface methodology (RSM) to maximize the laccase production. The predicted model showed maximum laccase activity of 402 U/mL appearing after 96 h of incubation with PM 2.0 g/L and FM 1.5 g/L at pH 7.0. Single protein band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) confirmed homogeneity of the laccase with a molecular weight of 63-75 kDa. The partially purified laccase effectively degraded the pesticides (Tricel, 71.8 ± 3.5 and Phoskill 77.3 ± 3.4%) within 5 days of incubation (40 °C) in pH 7.0. The pesticide degradation was further confirmed by high-performance liquid chromatography (HPLC) and the chromatograms showed the single dominant peaks at retention time 2.482 (tricel) and 2.608 (phoskill) min, respectively. Pesticide-degrading laccase was produced by Bacillus sp. PS under SSF reveals the utilization of low-cost bi-substrates for enhanced laccase production.

Study of Crystallographic Texture Evolution during High-Temperature Deformation of 18Cr-ODS Ferritic Steel based on Plasticity Assessment.

This paper aims at understanding the texture evolution in extruded oxide dispersion strengthened 18Cr ferritic steel during high-temperature uniaxial compression testing at 1,423 K at a strain rate of 0.01/s based on extensive electron back scatter diffraction characterization. The α-fiber texture is observed along the extrusion direction (ED) in the initial microstructure. The flow curves generated during uniaxial compression test are used to determine the associated hardening parameters. In addition, the degree of texture evolution after deformation along the ED and the transverse direction (TD) with respect to the initial condition has been predicted using VPSC-5 constitutive model. The prediction shows that the deformation along the ED produces a dominant γ-fiber texture in contrast to the TD. This is in agreement with the experimental results where γ-fiber texture is observed, due to enhanced dynamic recrystallization at high-temperature deformation.