Impacts of Exercise Interventions on Inflammatory Markers and Vascular Adhesion Molecules in Patients With Heart Failure: A Meta-analysis of RCTs.

Background: The aim of this meta-analysis was to investigate the effects of concurrent, aerobic, and resistance exercise on markers of inflammation and vascular adhesion molecules (high-sensitivity C-reactive protein [hs-CRP], interleukin [IL]-6, tumour necrosis factor-alpha [TNF-α], soluble intercellular adhesion molecule-1 [sICAM-1], soluble vascular cell adhesion molecule-1 [sVCAM-1], fibrinogen, IL-1-ß, IL-10, IL-18, and E-selectin) in patients with heart failure (HF). Methods: The PubMed, Scopus, Web of Science, and Google Scholar databases were searched for dates up to August 31, 2022. Randomized controlled trial studies for exercise interventions on circulating inflammatory and vascular adhesion markers in patients with HF were included. Standardized mean difference (SMD) and 95% confidence interval (CI) were calculated. Results: A total of 45 articles were included. Exercise training significantly reduced hs-CRP (SMD -0.441 [95% CI: -0.642 to -0.240], P = 0.001), IL-6 (SMD -0.158 (95% CI: -0.303 to -0.013], P = 0.032), and sICAM-1 (SMD -0.282 [95% CI: -0.477 to -0.086], P = 0.005) markers. Analysis of subgroups revealed that a significant reduction occurred in hs-CRP level for the following subgroups: middle-aged, elderly, overweight status, aerobic exercise, concurrent training, both high and moderate intensity, and short-term, long-term, and very long-term follow-up, compared to a control group (P < 0.05). A significant reduction occurred in IL-6 and sICAM-1 levels for those in the following subgroups, compared to a control group (P < 0.05): middle-aged, aerobic exercise, moderate-intensity exercise, and short-term follow-up. A reduction in TNF-α level occurred for middle-aged patients, compared to a control-group (P < 0.05). Conclusions: These exercise-related changes (improved inflammation and vascular adhesion markers) as clinical benefits in general, and for exercise-based cardiac rehabilitation in a more-specific format, improve clinical evolution and survival in patients with HF of different etiologies (registration number = CRD42021271423).

Contexte: L'objectif de cette méta-analyse était d'étudier les effets d'un entraînement combiné, aérobie ou musculaire sur les marqueurs de l'inflammation et sur les molécules d'adhésion des cellules vasculaires (protéine C-réactive à haute sensibilité [hs-CRP], interleukine 6 [IL-6], facteur de nécrose tumorale alpha [TNF-α], molécule d'adhésion intercellulaire de type 1 sous forme soluble [sICAM-1], molécule d'adhésion aux cellules vasculaires de type 1 sous forme soluble [sVCAM-1], fibrinogène, IL-1 bêta, IL-10, IL-18 et sélectine E) chez les patients souffrant d'insuffisance cardiaque. Méthodologie: Les bases de données PubMed, Scopus, Web of Science et Google Scholar ont été consultées jusqu'au 31 août 2022 pour relever les essais contrôlés randomisés traitant des effets de l'exercice physique sur les marqueurs inflammatoires circulants et les marqueurs d'adhésion vasculaire chez les patients souffrant d'insuffisance cardiaque. La différence moyenne standardisée (DMS) et l'intervalle de confiance (IC) à 95 % ont également été calculés. Résultats: Au total, 45 articles ont été retenus. L'entraînement physique a réduit de manière significative les marqueurs de hs-CRP (DMS : ­0,441 [IC à 95 % : ­0,642 à ­0,240], p = 0,001), IL-6 (DMS : ­0,158 (IC à 95 % : ­0,303 à ­0,013], p = 0,032) et sICAM-1 (DMS : ­0,282 [IC à 95 % : ­0,477 à ­0,086], p = 0,005). L'analyse des sous-groupes a révélé une réduction significative du taux de hs-CRP par rapport au groupe témoin (p < 0,05) pour les modalités suivantes : âge moyen, âge avancé, surpoids, entraînement aérobie, entraînement combiné, entraînement à intensité élevée ou modérée, suivi à court terme, à long terme et à très long terme. Une réduction significative des taux d'IL-6 et de sICAM-1 a également été observée par rapport au groupe témoin (p < 0,05) pour les modalités suivantes : âge moyen, entraînement aérobie, entraînement à intensité modérée et suivi à court terme. Enfin, une réduction du taux de TNF-α a été observée chez les patients d'âge moyen par rapport au groupe témoin (p < 0,05). Conclusions: Ces changements liés à l'exercice (amélioration des marqueurs d'inflammation et d'adhésion vasculaire), qui entraînent des bienfaits cliniques en général, mais plus particulièrement dans le cadre d'une réadaptation cardiaque, améliorent l'évolution clinique et la survie chez les patients souffrant d'insuffisance cardiaque de différentes causes (numéro d'enregistrement = CRD42021271423).

The role of exercise training on cardiovascular peptides in patients with heart failure: A systematic review and meta-analysis.

Background: The purpose of this systematic review & meta-analysis was to determine the roles of aerobic, resistance or concurrent exercises vs. control (CON) group on B-type natriuretic peptide (BNP) and N-terminal-pro hormone BNP (NT-proBNP) in patients with heart failure. Methods: The electronic databases of PubMed, Scopus, Web of Science, and Google Scholar were searched up to May 2022 for aerobic vs. CON, resistance vs. CON, and concurrent vs. CON studies on circulating (serum or plasma) levels of BNP and NT-proBNP in patients with heart failure. Non-randomized or randomized controlled trial studies were included. Standardized mean difference (SMD) and 95% confidence intervals (95% CIs) were calculated. This systematic review & meta-analysis was registered in PROSPERO at the University of York with the registration number [CRD42021271632]. Results: A total of 28 articles (37 intervention arms), 26 aerobic intervention arms, 3 resistance intervention arms, and 8 concurrent intervention arms were included. A total of 2563 participants (exercise groups = 1350 and CON groups = 1213) were included. Exercise training significantly decreased NT-proBNP marker [-0.229 (SMD and 95% CI: 0.386 to -0.071), p = 0.005], irrespective of overweight/obesity status. Analysis of subgroup by type of exercise training revealed that there was a significant reduction in the NT-proBNP marker for aerobic exercise group compared to the CON group [-0.336 (SMD and 95% CI: 0.555 to -0.105), p = 0.004], whereas concurrent exercise did not show significant changes in the NT-proBNP marker [-0.134 (SMD and 95% CI: 0.350 to 0.083), p = 0.227]. In addition, exercise training did not significantly change the BNP marker [-0.122 (SMD and 95% CI: 0.322 to 0.079), p = 0.235]. Conclusions: The results suggested that exercise training, especially aerobic exercise can be improved the NT-proBNP concentrations in patients with HF (irrespective of overweight/obesity status), which may be a sign of positive physiological adaptations to aerobic exercise.

Serum and gene expression profile of cytokines following combination of yoga training and vitamin D supplementation in breast cancer survivors: a randomized controlled trial.

BACKGROUND: This study aimed to examine the effect of the combination of yoga training with high vitamin D dose supplementation on the expression and systemic levels of inflammatory cytokines and psychophysical status of breast cancer survivors. METHODS: Thirty volunteered breast cancer survivors (48 ± 8 years) were randomly allocated to a high dose (4000 IU) of vitamin D supplementation (HD) group (n = 10), yoga with a high dose of vitamin D (YHD) group (n = 10), and yoga with a low dose (2000 IU) of vitamin D (YLD) group (n = 10). Participants performed the Hatha yoga style for 12 weeks, twice a week. Blood samples, quality of life (QoL) questionnaire, and physical performance tests were taken before and after the intervention. RESULTS: Body fat percentage (ηp2 = 0.36), handgrip strength (ηp2 = 0.41) and QoL indicators include global health (ηp2 = 0.54), functional scales (ηp2 = 0.49), and symptoms scales (ηp2 = 0.50) were significantly improved in the both YHD and YLD groups compared to the HD group (p < 0.05). Also, interleukin-10 (IL-10) levels were markedly increased in the Y-HVD group compared to the Y-LVD and HVD groups. Moreover, there were significant decreases in tumor necrosis factor-α (TNF-α) and interleukin-6 levels in the Y-HVD group after the intervention. The anti-inflammatory index (IL-10/TNF-α) was significantly increased in both the yoga groups (P < 0.05). CONCLUSION: Yoga promotes physical and psychological fitness and, in combination with a high dose of vitamin D, improves the cytokine profile, which can effectively manage the side effects associated with cancer. Trial registration IRCT20210726051993N2. Registration date: 2022/02/27. URL: https://www.irct.ir/trial/62079.

Effect of Stocking Density and Dietary Protein Level in Biofloc System on the Growth, Digestive and Antioxidant Enzyme Activities, Health, and Resistance to Acute Crowding Stress in Juvenile Common Carp (Cyprinus carpio).

This study is aimed at evaluating the effects of stocking densities and dietary protein levels in the biofloc system on the performance of common carp. Fish (12.09 ± 0.99 g) were transferred to 15 tanks: fish reared at 10 kg/m3 as medium density and fed 35% (MD35) or 25% (MD25) protein and fish reared at 20 kg/m3 as high density and fed 35% (HD35) or 25% (HD25) protein in the biofloc system and control fish reared at MD and fed 35% protein in clear water. After 60 days, fish were subjected to crowding stress (80 kg/m3) for 24 h. The growth of fish was highest in MD35. The feed conversion ratio was lower in MD35 compared to the control and HD groups. The amylase, lipase, protease, and superoxide dismutase and glutathione peroxidase activities in the biofloc groups were significantly higher than in the control. After crowding stress, cortisol and glucose levels in biofloc treatments were significantly decreased compared to the control. After 12 and 24 h stress, lysozyme activity in MD35 was significantly lower than in the HD treatments. Overall, the biofloc system with MD could improve growth and robustness against acute stress in fish. Also, biofloc could compensate 10% reduction of protein in common carp juvenile diet when reared in MD.

Biofloc technology improves growth, innate immune responses, oxidative status, and resistance to acute stress in common carp (Cyprinus carpio) under high stocking density.

This study aimed to evaluate the effects of biofloc technology (BFT) on the performance of common carp under high stocking density. Fish (8.41 ±â€¯0.44 g) were stocked into 12 tanks (70 L) in a 2 × 2 factorial design consisting of clear water (CW) and BFT systems at two stocking densities. Fish were divided into four groups according to culture system and stocking density: CW6 (clear water, 6 kg m-3), CW12 (clear water, 12 kg m-3), BFT6 (biofloc, 6 kg m-3), and BFT12 (biofloc, 12 kg m-3). After 49 days, the fish were exposed to acute crowding stress (40 kg m-3) for 7 days to evaluate the fish performance to additional stressors. Total ammonia nitrogen (TAN) concentration was significantly lower in BFT groups at the end of the trial. The growth of fish was significantly higher and the feed conversion ratio was lower in BFT groups. The final density of fish was highest in BFT12 group (25.41 ±â€¯0.48 kg m-3). The amylase and protease activities in BFT groups were significantly higher than the CW12 group. The highest serum catalase, superoxide dismutase, and glutathione peroxidase activities, and malondialdehyde content (3.07 ±â€¯0.16 nmol mg-1 protein) were observed in CW12 group. Serum LDL, cholesterol, triglyceride, total protein, and albumin levels were significantly higher in CW6 and BFT6 groups, followed by BFT12 group, and lowest in CW12 group. Serum globulin level was lowest in CW12 group (0.68 ±â€¯0.06 g dL-1). After 49 days, serum lysozyme, alternative complement (ACH50) and ALP activities were significantly higher and serum cortisol and glucose levels and ALT and AST activities were lower in BFT groups than the CW12 group. After 7 days of acute stress, serum lysozyme activities were significantly lower and ACH50 and ALP activities were higher in BFT groups than the CW12 group. Also, fish in CW12 group were more sensitive to the acute stressor, showing higher cortisol and lower glucose levels. Overall, biofloc had the anti-stress effect, and the culture of common carp in the biofloc system at a stocking density of 12 kg m-3 could be recommended. BFT can also exert beneficial effects on the welfare of fish subjected to acute stress.

Effects of chronic high stocking density on liver proteome of rainbow trout (Oncorhynchus mykiss).

The main aim of the present study was to assess the effects of chronic high stocking density on liver proteome of rainbow trout. Rainbow trout juveniles (42.6 ± 2.3 g average body weight) were randomly distributed into six tanks at two stocking densities (low stocking density (LD) = 20 kg m-3 and high stocking density (HD) = 80 kg m-3). Both treatments were performed in triplicate tanks for a period of 60 days. High stocking density caused a reduction in the growth performance compared with LD fish. Lysozyme activity increased with stocking density, while serum complement activity presented the opposite pattern. Serum cortisol and total protein levels did not show significant differences (P > 0.05) between experimental groups. The fish reared at high stocking density showed significantly lower osmolality and globulin values but higher albumin level. The HD group had significantly higher activities of catalase, glutathione peroxidase and superoxide dismutase, and malondialdehyde content in the liver when compared to the LD group. Comparative proteomics was used to determine the proteomic responses in livers of rainbow trout reared at high stocking density for 60 days. Out of nine protein spots showing altered abundance (>1.5-folds, P < 0.05), eight spots were successfully identified. Two proteins including apolipoprotein A-I-2 precursor and mitochondrial stress-70 protein were found to increase in HD group. The spots found to decrease in the HD group were identified as follows: 2-peptidylprolyl isomerase A, two isoforms of glyceraldehydes-3-phosphate dehydrogenase, an unnamed protein product similar to fructose-bisphosphate aldolase, 78 kDa glucose-regulated protein, and serum albumin 1 protein.

Proteomic analysis of liver tissue from rainbow trout (Oncorhynchus mykiss) under high rearing density after administration of dietary vitamin E and selenium nanoparticles.

The aim of the present study was to investigate the effects of dietary vitamin E (vit E) and selenium nanoparticles (nanoSe) on liver proteome profile of rainbow trout under high density condition. To correlate the proteome modifications with physiological aspects, growth, serum metabolites (cortisol, glucose, lactate, ALT, AST, and ALP), and liver antioxidant-related parameters (SOD, GPx, CAT, and MDA) were also examined. A total of 1275 fish (average weight of 42.6±2.3g) were stocked into 12 tanks at a density of 80kgm-3. The fish were divided into four groups according to diet: control (basal diet), vit E (500mgkg-1 vit E-supplemented diet), nanoSe (1mgkg-1 nanoSe-supplemented diet), and combination (500mgkg-1 vit E and 1mgkg-1 nanoSe-supplemented diet). After 60days, the best performance and health status of fish were observed in vit E and combination groups. Supplementation with nanoSe had no significant effects on growth performance. In addition, we compared liver proteome profiles of fish fed with a basal diet (control) and diets supplemented with vit E or nanoSe. Among the identified proteins, GRP78, ATPsyn-d, and HSP70 had an increased abundance in the vit E group, while HPPD and GAPDH showed a decreased abundance. In response to nanoSe supplementation, the expression of MDH, FAA, FBPA, TPI, GRHPR, GNMT, FDH, and Enol was increased. The proteomic data indicate that vit E or nanoSe supplementation can alter the expression of proteins involved in metabolic status of rainbow trout reared under high rearing density.

Design of a biological method for rapid detection of presence of PCR inhibitors in aged bone DNA.

BACKGROUND: Molecular human identification is one of the most important tests performed in forensic laboratories. Some of these tests are applied for identification of human remains from natural disasters, wars, etc., but problems may occur as a result of DNA degradation and external DNA contamination. We investigated effects of bacterial DNA on identifying the presence or absence of PCR inhibitors in aged bone DNA. METHODS: DNA samples were extracted from blood, bone remains and Escherichia coli. These DNA were amplified using human and bacterial specific primers. RESULTS: Using different blood, aged bone, and bacterial DNA dilutions along with PCR based methods; we checked their positive, negative effects, or detecting presence of inhibitors in aged bone DNA by PCR method. CONCLUSIONS: Our observation indicated that the addition of bacterial DNA could be a valid biological method for testing the quality of bone DNA to enable us to obtain a usable profile for the identification of human remains. This method will help to test the presence of inhibitors, quantity or even quality of DNA which are of importance in profiling archeological remains. Our method will help to determine if PCR failure is due to presence of inhibitors or lack of amplifiable DNA either because of degradation, minute amount or absence of human DNA.