RESUMEN
We report a case of clear cell carcinoma (CCC) of the ovary with plasma cell-rich inflammatory stroma, a recently proposed subtype of CCC, and present the cytological findings. The patient was a 48-year-old woman, who was incidentally found to have a right ovarian tumor during the preoperative work-up for an early-stage adenocarcinoma of the uterine cervix. Cytological examination of an imprint smear of the ovarian tumor and peritoneal washing revealed solid cell clusters of irregular, often dendritic shapes, which were intermingled with many inflammatory cells. "Raspberry bodies" were not found. Histopathological examination of the extirpated ovarian tumor showed the features of CCC with plasma cell-rich inflammatory stroma. This subtype of ovarian CCC poses cytological and histological diagnostic problems, and its differentiation from dysgerminoma is often difficult, because it mostly lacks the hyaline or mucoid stroma. Irregularly shaped clusters of large polyhedral cells, coarsely clumped nuclear chromatin, and plasma cell-rich inflammatory infiltrates suggest CCC, but the cytological differences between dysgerminoma and CCC are often subtle, and immunohistochemical examinations for cytokeratin 7 or epithelial membrane antigen may be necessary. Diagn. Cytopathol. 2017;45:128-132. © 2016 Wiley Periodicals, Inc.
Asunto(s)
Carcinoma/patología , Neoplasias Ováricas/patología , Células del Estroma/patología , Femenino , Humanos , Persona de Mediana Edad , Prueba de PapanicolaouRESUMEN
Metastasis from various neoplasms to the spleen is very rare and most of the cases are found at autopsy. We report a patient presenting with uterine cervical cancer with splenic metastases. A 49-year-old woman presenting with genital bleeding was referred to our hospital and diagnosed with stage IIB cervical cancer. She underwent concomitant chemoradiotherapy (CCRT) consisting of 50 Gy whole pelvis irradiation, high-dose-rate intracavitary brachytherapy 24 Gy/4 fractions and six weeks of paclitaxel and carboplatin administration. Ten months after the initial therapy, CT revealed recurrence at spleen. Although she received 5 courses of nedaplatin, enlargement of the tumor was noticed. She underwent a splenectomy and the result of histology was compatible with metastasis of cervical cancer.
RESUMEN
Malignant changes in endometriosis are often reported, but those in adenomyosis are rare. We report a case of endometrioid adenocarcinoma arising from adenomyosis. Case Presentation. A 57-year-old woman presenting with vaginal bleeding was referred to our hospital. Cytological tests of endometrium revealed atypical glandular cells. Fractional endometrial curettage revealed normal endometrium without atypia. Magnetic resonance imaging (MRI) revealed multiple myomas. The endometrium was slightly enhanced on T1-weighted imaging and endometrial cancer was suspected. Myometrial invasion was not evident. The patient was admitted and semiradical hysterectomy with bilateral salpingo-oophorectomy and pelvic lymphadenectomy was performed. Histopathological study revealed grade 1 endometrioid adenocarcinoma. Although the lesion was located in the muscle layer of the corpus and invaded more than half of it, the endometrium was intact. Pelvic lymph node metastasis was noticed. No cervical invasion or metastasis to the adnexa was seen. We diagnosed the case with a stage 1B endometrioid adenocarcinoma originating from adenomyosis. Adjuvant chemotherapy was then performed in the form of 5 cycles of paclitaxel (180 mg/m(2)) and carboplatin (AUC = 5). Five years later, right lung metastasis and right para-aortic and pelvic lymph nodes metastasis were noticed. Paclitaxel and carboplatin are now being administered.
RESUMEN
Adenosarcoma is a rare tumor which consists of benign glandular epithelium and malignant mesenchymal component. Here we report a case of adenosarcoma of the uterine corpus. Case Presentation. A 59-year-old woman presented with vaginal bleeding and visited a local clinic. She had a uterine tumor pointed out and was referred to our hospital. Ultrasound scans revealed a large heterogeneous mass occupying the whole uterine cavity. Cytological test of endometrium was performed but the result was negative. A fractional endometrial curettage revealed no malignancy. Magnetic resonance imaging (MRI) revealed a heterogeneous solid tumor of 77 × 76 mm. Total abdominal hysterectomy with bilateral salpingo-oophorectomy and pelvic lymphadenectomy was performed. On gross examination, the tumor was arising from the uterine body and occupied the whole uterine cavity. Histopathological examination revealed phyllodes-like architecture on low magnificationandperiglandular cuffing of tumor cells. The lesion was confined to the uterus. Histopathological final diagnosis was adenosarcoma. Her postoperative course was uneventful and she was discharged without postoperative treatment and remains alive without disease 6 months after the surgery.
RESUMEN
Placental mesenchymal dysplasia (PMD) rarely complicates with pregnancy. A 30-year-old woman, gravida 3, para 3, presenting with placentomegaly, was referred to our department at 18 weeks of gestation. An ultrasonography revealed a normal fetus with a large multicystic placenta, measuring 125 × 42 × 80 mm. The border between the lesion and normal region was not clear. Color doppler revealed little blood flow in the lesion. Magnetic resonance imaging revealed normal fetus and a large multicystic placenta. Serum human chorionic gonadotropin level was 20124.97 U/L, which was normal at 20 weeks of gestation. Thus, placental mesenchymal dysplasia rather than hydatidiform mole with coexistent fetus was suspected. Then, routine checkup was continued. Because she had the history of Cesarean section, an elective Cesarean section was performed at 37 weeks of gestation, and 2520 g female infant with apgar score 8/9 was delivered. The baby was normal with no evidence of Beckwith-Wiedemann syndrome. Placenta of 20 × 16 × 2 cm, weighing 720 g, was bulky with grape like vesicles involving whole placenta. Microscopic examination revealed dilated villi and vessels with thick wall which was lacking trophoblast proliferation. Large hydropic stem villi with myxomatous struma and cistern formation were seen. PMD was histopathologically confirmed.
Asunto(s)
Benzoquinonas/aislamiento & purificación , Naftoquinonas/aislamiento & purificación , Streptomyces/química , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Benzoquinonas/química , Benzoquinonas/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Fermentación , Células HeLa , Humanos , Espectroscopía de Resonancia Magnética , Estructura Molecular , Naftoquinonas/química , Naftoquinonas/farmacologíaRESUMEN
A novel naphthoquinone-like polyketide, JBIR-85 (1), with a unique skeleton and antioxidative activity was isolated from a culture of Streptomyces sp. RI-77. The planar structure of 1 was established on the basis of extensive NMR and MS analyses. The structure of 1 including the absolute configuration was established via X-ray crystallographic analysis. Since 1 exhibits a unique skeleton, we performed feeding experiments to reconfirm the structure and predict the biosynthetic pathway.
Asunto(s)
Depuradores de Radicales Libres/aislamiento & purificación , Naftoquinonas/aislamiento & purificación , Policétidos/aislamiento & purificación , Streptomyces/química , Compuestos de Bifenilo/farmacología , Cristalografía por Rayos X , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Conformación Molecular , Estructura Molecular , Naftoquinonas/química , Resonancia Magnética Nuclear Biomolecular , Picratos/farmacología , Policétidos/químicaRESUMEN
In the course of our screening program for anticancer agents from natural sources, five new angucyclines, JBIR-90 (1), -116 (2), -91 (3), -92 (4) and -93 (5), were isolated from the fermentation broth of Streptomyces sp. RI33. The structures of 1-5 were elucidated on the basis of 1D and 2D NMR spectroscopy and MS analyses. Compounds 2-4 showed cytotoxic activities against malignant pleural mesothelioma cells at IC(50) values of 20-50 µM.
Asunto(s)
Antineoplásicos/farmacología , Glicósidos/farmacología , Mesotelioma/tratamiento farmacológico , Neoplasias Pleurales/tratamiento farmacológico , Streptomyces/metabolismo , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Línea Celular Tumoral , Fermentación , Glicósidos/química , Glicósidos/aislamiento & purificación , Humanos , Concentración 50 Inhibidora , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Mesotelioma/patología , Neoplasias Pleurales/patologíaRESUMEN
A Gram-positive actinobacterium, designated M24DS4(T), was isolated from a soil sample collected from Aomi, Tokyo, Japan, using the membrane-filter method. Strain M24DS4(T) exhibited low 16S rRNA gene sequence similarity (96.1â%) with Streptomyces scabrisporus NBRC 100760(T). Cell hydrolysates contained the ll-isomer of diaminopimelic acid and the predominant quinones were MK-9(H(8)) and MK-9(H(6)). The genomic DNA G+C content was 75 mol%. Comparison of the characteristics of strain M24DS4(T) and related members of the genus Streptomyces with validly published names showed that the strain represents a novel species of the genus, for which the name Streptomyces aomiensis sp. nov. is proposed. The type strain is M24DS4(T) (â=âNBRC 106164(T) â=âKACC 14925(T)).
Asunto(s)
Microbiología del Suelo , Streptomyces/clasificación , Streptomyces/aislamiento & purificación , Composición de Base , Análisis por Conglomerados , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Ácido Diaminopimélico/análisis , Filtración/métodos , Datos de Secuencia Molecular , Filogenia , Quinonas/análisis , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Streptomyces/genética , TokioAsunto(s)
Naftoquinonas/aislamiento & purificación , Naftoquinonas/farmacología , Streptomyces/química , Línea Celular , Supervivencia Celular/efectos de los fármacos , Antagonistas de Aminoácidos Excitadores , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Glutamatos/metabolismo , Humanos , Naftoquinonas/química , Resonancia Magnética Nuclear Biomolecular , Rotación Óptica , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría Infrarroja , Espectrofotometría Ultravioleta , Terpenos/química , Terpenos/farmacologíaRESUMEN
Screening for anti-influenza virus activity in compounds isolated from Streptomyces sp. RI18, which was isolated using the membrane filter method, uncovered a novel compound, JBIR-68 (1), which contains a unique skeleton. Its structure was established by extensive NMR and MS analyses. In addition, 1 was synthesized to confirm the configuration of its sugar moiety. Compound 1 inhibited influenza virus growth in plaque assays.
Asunto(s)
Antivirales/química , Monosacáridos/química , Orthomyxoviridae/efectos de los fármacos , Pirimidinonas/química , Streptomyces/química , Antivirales/aislamiento & purificación , Antivirales/farmacología , Estructura Molecular , Monosacáridos/aislamiento & purificación , Monosacáridos/farmacología , Pirimidinonas/aislamiento & purificación , Pirimidinonas/farmacologíaAsunto(s)
Depuradores de Radicales Libres/aislamiento & purificación , Péptidos Cíclicos/aislamiento & purificación , Animales , Línea Celular , Línea Celular Tumoral , Ensayos de Selección de Medicamentos Antitumorales , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Humanos , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Neuronas/efectos de los fármacos , Fármacos Neuroprotectores/química , Fármacos Neuroprotectores/aislamiento & purificación , Fármacos Neuroprotectores/farmacología , Péptidos Cíclicos/química , Péptidos Cíclicos/farmacología , Ratas , Streptomyces/química , Streptomyces/genéticaAsunto(s)
Antifúngicos/aislamiento & purificación , Aspergillus/química , Furanos/aislamiento & purificación , Pirroles/aislamiento & purificación , Antifúngicos/química , Antifúngicos/farmacología , Candida albicans/efectos de los fármacos , Ensayos de Selección de Medicamentos Antitumorales , Sinergismo Farmacológico , Furanos/química , Furanos/farmacología , Humanos , Espectroscopía de Resonancia Magnética , Miconazol/administración & dosificación , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Pirroles/química , Pirroles/farmacologíaAsunto(s)
Penicillium/química , Penicillium/clasificación , Piridonas/química , Piridonas/aislamiento & purificación , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Espectroscopía de Resonancia Magnética , Filogenia , Piridonas/farmacología , Análisis de Secuencia de ADNRESUMEN
In the course of our chemical screening program for new secondary metabolites, we isolated new compounds JBIR-37 (1) and -38 (2) from a culture broth of the marine sponge-derived fungus, Acremonium sp. SpF080624G1f01. The structures of 1 and 2 were determined to be di- and mono-O-beta-D-glucopyranosyloxy-4-(1,1-dimethyl-2-propenyl)benzene on the basis of extensive NMR and MS spectroscopic data, respectively.
Asunto(s)
Acremonium/química , Glucósidos/aislamiento & purificación , Éteres Fenílicos/aislamiento & purificación , Poríferos/microbiología , Animales , Medios de Cultivo , Glucósidos/química , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Estructura Molecular , Éteres Fenílicos/químicaRESUMEN
In the course of our screening program for novel metabolites from tunicate-derived fungi, novel sesquiterpenoids, named JBIR-27 (1) and -28 (2), together with known sporogen-AO1 and phomenone, were isolated from the culture broth of Penicillium sp. SS080624SCf1. The structures of 1 and 2 were determined to be eremophilane analogs on the basis of extensive NMR and MS analyses. Sporogen-AO1, phomenone and 2 showed cytotoxicity against human cervical carcinoma cell line HeLa at IC(50) values of 8.3, 19 and 92 microM, respectively, whereas 1 was inactive at a concentration of 80 microM.
Asunto(s)
Antibióticos Antineoplásicos/aislamiento & purificación , Penicillium/química , Penicillium/metabolismo , Sesquiterpenos/aislamiento & purificación , Urocordados/microbiología , Animales , Antibióticos Antineoplásicos/farmacología , Medios de Cultivo , ADN de Hongos/biosíntesis , ADN de Hongos/metabolismo , Fermentación , Células HeLa , Humanos , Espectrometría de Masas , Conformación Molecular , Sesquiterpenos/farmacología , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
In the course of our screening program for active compounds that induce cell morphological changes of Saccharomyces cerevisiae, the culture broth of an entomopathogenic fungus Metarhizium sp. fE61 exhibited a unique morphological phenotype. We conducted an activity-guided isolation from the fermentation broth of Metarhizium sp. fE61 to yield two new macrolide compounds named JBIR-19 (1) and -20 (2) as active substances. Their structures were determined to be 24-membered macrolide analogs containing a 2-aminoethyl phosphate ester on the basis of NMR and other spectroscopic data. Compounds 1 and 2 induced striking elongated morphology of S. cerevisiae at concentrations of 3.1 and 13 microM, but showed weak antiyeast activity at MICs of 200 and >200 microM, respectively.