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The original article has been corrected.
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To elucidate mutation spectrum and genotype-phenotype correlations in Japanese patients with OI, we conducted comprehensive genetic analyses using NGS, as this had not been analyzed comprehensively in this patient population. Most mutations were located on COL1A1 and COL1A2. Glycine substitutions in COL1A1 resulted in the severe phenotype. INTRODUCTION: Most cases of osteogenesis imperfecta (OI) are caused by mutations in COL1A1 or COL1A2, which encode α chains of type I collagen. However, mutations in at least 16 other genes also cause OI. The mutation spectrum in Japanese patients with OI has not been comprehensively analyzed, as it is difficult to identify using classical Sanger sequencing. In this study, we aimed to reveal the mutation spectrum and genotype-phenotype correlations in Japanese patients with OI using next-generation sequencing (NGS). METHODS: We designed a capture panel for sequencing 15 candidate OI genes and 19 candidate genes that are associated with bone fragility or Wnt signaling. Using NGS, we examined 53 Japanese patients with OI from unrelated families. RESULTS: Pathogenic mutations were detected in 43 out of 53 individuals. All mutations were heterozygous. Among the 43 individuals, 40 variants were identified including 15 novel mutations. We found these mutations in COL1A1 (n = 30, 69.8%), COL1A2 (n = 12, 27.9%), and IFITM5 (n = 1, 2.3%). Patients with glycine substitution on COL1A1 had a higher frequency of fractures and were more severely short-statured. Although no significant genotype-phenotype correlation was observed for bone mineral density, the trabecular bone score was significantly lower in patients with glycine substitutions. CONCLUSION: We identified pathogenic mutations in 81% of our Japanese patients with OI. Most mutations were located on COL1A1 and COL1A2. This study revealed that glycine substitutions on COL1A1 resulted in the severe phenotype among Japanese patients with OI.
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Osteogénesis Imperfecta/genética , Adolescente , Adulto , Densidad Ósea/genética , Niño , Preescolar , Colágeno Tipo I/genética , Cadena alfa 1 del Colágeno Tipo I , Femenino , Estudios de Asociación Genética , Variación Genética , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Lactante , Japón , Masculino , Mutación , Análisis de Secuencia de ADN , Adulto JovenRESUMEN
Mandibuloacral dysplasia (MAD) is a rare autosomal recessive progeroid syndrome, characterized by mandibular hypoplasia, acroosteolysis affecting distal phalanges and clavicles, delayed closure of the cranial sutures, atrophic skin, and lipodystrophy. Recently, mutations in lamin A/C (LMNA) and zinc metalloprotease (ZMPSTE24), involved in post-translational processing of prelamin A to mature lamin A, have been identified in MAD kindreds. We now report novel compound heterozygous mutations in exon 1 (c.121C>T; p.Q41X) and exon 6 (c.743C>T; p.P248L) in ZMPSTE24 in two Japanese sisters, 7- and 3-year old, with severe MAD and characteristic facies and atrophic skin. The older sister had lipodystrophy affecting the chest and thighs but sparing abdomen. Their parents and a brother, who were healthy, had heterozygous mutations. The missense mutation, P248L, was not found in 100 normal subjects of Japanese origin. The mutant Q41X was inactive in a yeast halo assay; however, the mutant P248L retained near normal ZMPSTE24 activity. Immunoblots demonstrated accumulation of prelamin A in the patients' cell lysates from lymphoblasts. The lymphoblasts from the patients also revealed less intense staining for lamin A/C on immunofluorescence. We conclude that ZMPSTE24 deficiency results in accumulation of farnesylated prelamin A, which may be responsible for cellular toxicity and the MAD phenotype.
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Anomalías Múltiples/genética , Mandíbula/anomalías , Proteínas de la Membrana/genética , Metaloendopeptidasas/genética , Mutación Missense , Pueblo Asiatico , Niño , Preescolar , Análisis Mutacional de ADN , Femenino , Genotipo , Humanos , Lamina Tipo A , Lipodistrofia/genética , Proteínas de la Membrana/deficiencia , Metaloendopeptidasas/deficiencia , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Fenotipo , Prenilación , Precursores de Proteínas/genética , Precursores de Proteínas/metabolismo , HermanosRESUMEN
We previously reported that a type II sodium phosphate (Na(+)-Pi) cotransporter (Npt2) protein is expressed in osteoclasts and that Pi limitation decreases osteoclast-mediated bone resorption in vitro. We also demonstrated that mice homozygous for the disrupted Npt2 gene (Npt2-/-) exhibit a unique age-dependent bone phenotype that is associated with significant hypophosphatemia. In the present study, we sought to identify the Npt2 cDNA in mouse osteoclasts and characterize the impact of Npt2 gene ablation on osteoclast function and bone histomorphometry. We demonstrate that the osteoclast Npt2 cDNA sequence is identical to that of the proximal renal tubule and, thus, not an isoform or splice variant thereof. Histomorphometric analysis revealed that, at 25 days of age, Npt2-/- mice exhibited a reduction in osteoclast number and eroded perimeters, relative to wild-type mice. Moreover, although the number of metaphyseal trabeculae was reduced in 25-day-old Npt2-/- mice, trabecular bone volume was normal due to increased trabecular width. At 115 days of age, the decrease in osteoclast index persisted in Npt2-/- mice relative to wild-type littermates. However, mineralizing and osteoblast surfaces and bone formation rates were increased, and, although trabecular number was still reduced, trabecular bone volume was higher than that of wild-type mice. These data demonstrate a link between osteoclast activity and trabecular development in young Npt2-/- mice, and suggest that an age-related adaptation to Npt2 deficiency is apparent in osteoclast and osteoblast function and bone formation.
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Resorción Ósea/genética , Hipofosfatemia/genética , Osteoclastos/fisiología , Simportadores/genética , Animales , Células Cultivadas , Clonación Molecular , ADN Complementario , Femenino , Expresión Génica/fisiología , Homeostasis/fisiología , Túbulos Renales Proximales/metabolismo , Macrófagos/citología , Masculino , Ratones , Ratones Noqueados , Osteoclastos/citología , Fenotipo , Fosfatos/metabolismo , ARN Mensajero/análisis , Conejos , Proteínas Cotransportadoras de Sodio-Fosfato , Proteínas Cotransportadoras de Sodio-Fosfato de Tipo I , Proteínas Cotransportadoras de Sodio-Fosfato de Tipo II , Proteínas Cotransportadoras de Sodio-Fosfato de Tipo III , Simportadores/metabolismo , Tibia/citología , Tibia/fisiologíaRESUMEN
Migration of endothelial cells induced by vascular endothelial growth factor (VEGF) is a critical step in angiogenesis. Stimulation of motility by growth factors such as VEGF requires interaction with the signal transduction pathways activated by the extracellular matrix (ECM). Here we demonstrate that the Rac GTPase is the critical intersection activated by type 1 collagen ECM and VEGF during stimulation of endothelial cell motility. To analyze the role of the Rho family GTPases in VEGF-stimulated endothelial cell chemotaxis and ECM-stimulated haptotaxis, we transduced the respective fusion proteins in human foreskin dermal endothelial cells using a Tat peptide from the human immunodeficiency virus Tat protein. VEGF signaling required Rac activation during chemotaxis, and Rac and Cdc42 were activated during haptotaxis on type I collagen. Similar to VEGF, Rac activation induced an increase in endothelial cell stress fiber and focal adhesion. Surprisingly, Rho activation was not present in collagen-induced haptotaxis or stimulation of chemotaxis by VEGF, although Rho induced stress fibers and focal adhesions similar to Rac activation. The result of constitutive Rho activation was an inhibition of haptotaxis. Thus, Rac is required and sufficient for the activation of endothelial cell haptotaxis and VEGF-stimulated chemotaxis.
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Movimiento Celular/fisiología , Factores de Crecimiento Endotelial/metabolismo , Endotelio Vascular/enzimología , Linfocinas/metabolismo , Neovascularización Fisiológica/fisiología , Proteínas de Unión al GTP rho/metabolismo , Actinas/efectos de los fármacos , Actinas/metabolismo , Antimaláricos/farmacología , Movimiento Celular/efectos de los fármacos , Células Cultivadas/efectos de los fármacos , Células Cultivadas/metabolismo , Quimiotaxis/efectos de los fármacos , Quimiotaxis/fisiología , Cloroquina/farmacología , Colágeno/efectos de los fármacos , Colágeno/metabolismo , Citoesqueleto/efectos de los fármacos , Citoesqueleto/metabolismo , Factores de Crecimiento Endotelial/farmacología , Endotelio Vascular/efectos de los fármacos , Matriz Extracelular/efectos de los fármacos , Matriz Extracelular/metabolismo , Técnica del Anticuerpo Fluorescente , Productos del Gen tat/genética , Humanos , Linfocinas/farmacología , Neovascularización Fisiológica/efectos de los fármacos , Proteínas Recombinantes de Fusión/genética , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial Vascular , Vinculina/efectos de los fármacos , Vinculina/metabolismo , Proteína de Unión al GTP cdc42/metabolismo , Proteínas de Unión al GTP rac/efectos de los fármacos , Proteínas de Unión al GTP rac/metabolismo , Proteínas de Unión al GTP rho/efectos de los fármacosRESUMEN
Estrogen deficiency induces bone loss by upregulating osteoclastogenesis by mechanisms not completely defined. We found that ovariectomy-enhanced T-cell production of TNF-alpha, which, acting through the TNF-alpha receptor p55, augments macrophage colony-stimulating factor-induced (M-CSF-induced) and RANKL-induced osteoclastogenesis. Ovariectomy failed to induce bone loss, stimulate bone resorption, or increase M-CSF- and RANKL-dependent osteoclastogenesis in T-cell deficient mice, establishing T cells as essential mediators of the bone-wasting effects of estrogen deficiency in vivo. These findings demonstrate that the ability of estrogen to target T cells, suppressing their production of TNF-alpha, is a key mechanism by which estrogen prevents osteoclastic bone resorption and bone loss.
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Antígenos CD/metabolismo , Resorción Ósea/metabolismo , Proteínas Portadoras/metabolismo , Estrógenos/fisiología , Glicoproteínas de Membrana/metabolismo , Receptores del Factor de Necrosis Tumoral/metabolismo , Linfocitos T/metabolismo , Factor de Necrosis Tumoral alfa/biosíntesis , Animales , Antígenos CD/genética , Células Cultivadas , Factor Estimulante de Colonias de Macrófagos/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Ratones Desnudos , Osteoclastos/fisiología , Ovariectomía , Ligando RANK , Receptor Activador del Factor Nuclear kappa-B , Receptores del Factor de Necrosis Tumoral/genética , Receptores Tipo I de Factores de Necrosis Tumoral , Receptores Tipo II del Factor de Necrosis TumoralRESUMEN
We report the p35 and p60 forms of XRCC4 protein, appearing in human leukemia MOLT-4 or U937 cells following X-irradiation or hyperthermia. p35 appeared in conjunction with the cleavage of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and the fragmentation of internucleosomal DNA, and was suppressed by Ac-DEVD-CHO. p35 was also produced in vitro by treating MOLT-4 cell lysate with recombinant caspases, suggesting that p35 was a caspase-cleaved fragment of XRCC4 in apoptotic cell death. p60 was sensitive to treatment with phosphatase or wortmannin and was undetectable in M059J cells deficient in DNA-PKcs. However, p60 was found in ataxia-telangiectasia cells after irradiation. These results indicated p60 as a phosphorylated form of XRCC4, requiring DNA-PKcs but not ataxia-telangiectasia mutated (ATM).
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Proteínas de Unión al ADN/metabolismo , Proteínas de Unión al ADN/efectos de la radiación , Androstadienos/farmacología , Proteínas de la Ataxia Telangiectasia Mutada , Western Blotting , Caspasa 3 , Caspasa 7 , Inhibidores de Caspasas , Caspasas/metabolismo , Proteínas de Ciclo Celular , Proteína Quinasa Activada por ADN , Proteínas de Unión al ADN/química , Calor , Humanos , Peso Molecular , Proteínas Nucleares , Fragmentos de Péptidos/química , Fragmentos de Péptidos/metabolismo , Fragmentos de Péptidos/efectos de la radiación , Fosfatidilinositol 3-Quinasas/metabolismo , Inhibidores de las Quinasa Fosfoinosítidos-3 , Fosforilación/efectos de la radiación , Proteínas Serina-Treonina Quinasas/antagonistas & inhibidores , Proteínas Serina-Treonina Quinasas/deficiencia , Proteínas Serina-Treonina Quinasas/genética , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Recombinantes/antagonistas & inhibidores , Proteínas Recombinantes/metabolismo , Células Tumorales Cultivadas , Proteínas Supresoras de Tumor , Células U937 , WortmaninaRESUMEN
Osteoclasts express the alphavbeta3 integrin, an adhesion receptor that has been implicated in bone resorption and that is therefore a potential therapeutic target. To assess the role of this heterodimer in skeletal development in vivo, we engineered mice in which the gene for the beta3 integrin subunit was deleted. Bone marrow macrophages derived from these mutants differentiate in vitro into numerous osteoclasts, thus establishing that alphavbeta3 is not necessary for osteoclast recruitment. Furthermore, the closely related integrin, alphavbeta5, does not substitute for alphavbeta3 during cytokine stimulation or authentic osteoclastogenesis. beta3 knockout mice, but not their heterozygous littermates, develop histologically and radiographically evident osteosclerosis with age. Despite their increased bone mass, beta3-null mice contain 3.5-fold more osteoclasts than do heterozygotes. These mutant osteoclasts are, however, dysfunctional, as evidenced by their reduced ability to resorb whale dentin in vitro and the significant hypocalcemia seen in the knockout mice. The resorptive defect in beta3-deficient osteoclasts may reflect absence of matrix-derived intracellular signals, since their cytoskeleton is distinctly abnormal and they fail to spread in vitro, to form actin rings ex vivo, or to form normal ruffled membranes in vivo. Thus, although it is not required for osteoclastogenesis, the integrin alphavbeta3 is essential for normal osteoclast function.
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Antígenos CD/genética , Resorción Ósea/genética , Cadenas beta de Integrinas , Osteoclastos/citología , Osteosclerosis/genética , Glicoproteínas de Membrana Plaquetaria/genética , Animales , Células de la Médula Ósea/citología , Diferenciación Celular , Movimiento Celular , Matriz Extracelular/metabolismo , Fémur/citología , Integrina beta3 , Integrinas/metabolismo , Macrófagos/citología , Ratones , Ratones Noqueados , Osteoclastos/metabolismo , Transducción de SeñalRESUMEN
The integrins alphavbeta3 and alphavbeta3 are expressed reciprocally during murine osteoclastogenesis in vitro. Specifically, immature osteoclast precursors, in the form of bone marrow macrophages, contain exclusively alphavbeta5, surface expression of which declines with commitment to the osteoclast phenotype, while levels of alphavbeta3 increase concomitantly. The distinct functional significance of alphavbeta5 is underscored by the integrin's capacity, unlike alphavbeta3, to mediate both attachment and spreading on ligand, of marrow macrophages, suggesting alphavbeta3 negotiates initial recognition, by osteoclast precursors, of bone matrix. Northern analysis demonstrates changes in the two beta-subunits, and not alphav, are responsible for these alterations. Treatment of early precursors with granulocyte-macrophage colony stimulating factor (GM-CSF) leads to alterations in beta3 and beta5 mRNA and alphavbeta5 and alphavbeta3, paralleling those occurring during osteoclastogenesis. Nuclear run-on and message stability studies demonstrate that while GM-CSF treatment of precursors alters beta5 transcriptionally, the changes in beta3 arise from prolonged mRNA t1/2. Similar to GM-CSF treatment, the rate of beta5 transcription falls during authentic osteoclastogenesis. In contrast to cytokine-induced alphavbeta3, however, that attending osteoclastogenesis reflects accelerated transcription of the beta3-subunit. Thus, while GM-CSF may participate in modulation of alphavbeta5 during osteoclast differentiation, signals other than those derived from the cytokine must regulate expression of alphavbeta3.
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Regulación de la Expresión Génica , Factor Estimulante de Colonias de Granulocitos y Macrófagos/farmacología , Integrinas/genética , Osteoclastos/metabolismo , Receptores de Vitronectina/genética , Células Madre/metabolismo , Animales , Membrana Celular/metabolismo , Integrinas/fisiología , Cinética , Masculino , Ratones , Ratones Endogámicos C3H , ARN Mensajero/metabolismo , Receptores de Vitronectina/fisiología , Transcripción GenéticaAsunto(s)
Transportadoras de Casetes de Unión a ATP , Adenosina Trifosfato/farmacología , Canales de Potasio de Rectificación Interna , Canales de Potasio/efectos de los fármacos , Canales de Potasio/genética , Animales , Clonación Molecular , Conductividad Eléctrica , Humanos , Isomerismo , Canales de Potasio/metabolismo , Canales de Potasio/fisiología , Receptores de Droga/genética , Receptores de Droga/fisiología , Receptores de SulfonilureasRESUMEN
Inwardly rectifying K+ (Kir) channels play important roles in various cellular functions in excitable and non-excitable cells. We recently cloned the human genes encoding the Kir channel subunits Kir2.2v (KCNJN1) and Kir2.2 (KCNJ12). However, the physiological role of Kir2.2v has not yet been clarified. Fluorescence in situ hybridization analysis of human metaphase chromosomes assigned both genes to 17p11.2-->p11.1. The presence of hybridization signals in the paracentromeric regions of both chromosomes 17 from two Smith-Magenis syndrome (SMS) patients indicated that Kir2.2v and Kir2.2 are not located within the minimum critical region of this syndrome.
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Anomalías Múltiples/genética , Mapeo Cromosómico , Cromosomas Humanos Par 17 , Discapacidad Intelectual/genética , Canales de Potasio de Rectificación Interna , Canales de Potasio/genética , Análisis Mutacional de ADN , Humanos , Hibridación Fluorescente in Situ , Reacción en Cadena de la PolimerasaRESUMEN
We have cloned the human genes encoding the inwardly rectifying K+ (Kir) channel subunits, Kir2.2 (hKir2.2) and its variant, termed hKir2.2v. When expressed in Xenopus oocytes, hKir2.2 produced strong inwardly rectifying K+ currents, whereas the expression of hKir2.2v did not elicit significant currents. Coexpression of hKir2.2v with hKir2.2 showed an hKir2.2v inhibition of hKir2.2 K+ currents, indicating that it acts as a negative regulator of hKir2.2 channel activity. Mutational analysis of hKir2.2v and studies of chimeras between hKir2.2 and hKir2.2v suggest that the intracellular C-terminal region of hKir2.2v participates in the negative regulation of the hKir2.2v channel activity.
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Canales de Potasio de Rectificación Interna , Canales de Potasio/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario , Electrofisiología , Humanos , Datos de Secuencia Molecular , Mutación , Oocitos , Canales de Potasio/genética , Proteínas Recombinantes de Fusión/genética , Homología de Secuencia de Aminoácido , XenopusRESUMEN
We have isolated cDNA encoding a fourth member (P2X-4) of the ATP receptor P2X receptor family from a rat pancreatic islet cDNA library. Rat P2X-4 is a protein of 388 amino acids which shares 50%, 49%, and 47% identity with P2X-1, P2X-2, and P2X-3, respectively, and has two putative transmembrane segments. Rat P2X-4 mRNA is widely expressed in brain and peripheral tissues, including various endocrine tissues, and it is also expressed in various hormone-secreting cell lines. We have heterologously expressed the cloned P2X-4 in Xenopus laevis oocytes and have characterized its pharmacological properties. ATP, its analogs and ADP activate cation-selective ion channels. The order of agonist potency is ATP ADP 2-methyl- thioATP(2MeSATP) >> alpha beta-methelene-ATP (alpha betameATP). ATP-evoked currents are only partially blocked by suramin, reactive blue-2, or H2DIDS. The present study suggests that P2X-4, with pharmacological properties distinct from those of P2X-1+, P2X-2, and P2X-3, mediates extracellular ATP-induced biological effects in non-neuronal cells, including endocrine cells, as well as in neuronal cells.
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Encéfalo/metabolismo , Glándulas Endocrinas/metabolismo , Receptores Purinérgicos P2/clasificación , Receptores Purinérgicos P2/genética , Secuencia de Aminoácidos , Animales , Clonación Molecular , ADN Complementario/genética , Femenino , Expresión Génica , Técnicas In Vitro , Masculino , Datos de Secuencia Molecular , Oocitos/metabolismo , Ratas , Receptores Purinérgicos P2/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Homología de Secuencia de Aminoácido , Distribución Tisular , Xenopus laevisRESUMEN
A member of the inwardly rectifying potassium channel family was cloned here. The channel, called BIR (Kir6.2), was expressed in large amounts in rat pancreatic islets and glucose-responsive insulin-secreting cell lines. Coexpression with the sulfonylurea receptor SUR reconstituted an inwardly rectifying potassium conductance of 76 picosiemens that was sensitive to adenosine triphosphate (ATP) (IKATP) and was inhibited by sulfonylureas and activated by diazoxide. The data indicate that these pancreatic beta cell potassium channels are a complex composed of at least two subunits--BIR, a member of the inward rectifier potassium channel family, and SUR, a member of the ATP-binding cassette superfamily. Gene mapping data show that these two potassium channel subunit genes are clustered on human chromosome 11 at position 11p15.1.
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Transportadoras de Casetes de Unión a ATP , Canales de Potasio de Rectificación Interna , Canales de Potasio/química , Canales de Potasio/metabolismo , Potasio/metabolismo , Receptores de Droga/química , Adenosina Trifosfato/farmacología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Línea Celular , Mapeo Cromosómico , Cromosomas Humanos Par 11 , Clonación Molecular , Cricetinae , Diazóxido/farmacología , Humanos , Islotes Pancreáticos/metabolismo , Canales KATP , Ratones , Datos de Secuencia Molecular , Canales de Potasio/genética , Ratas , Receptores de Droga/metabolismo , Rubidio/metabolismo , Compuestos de Sulfonilurea/farmacología , Receptores de SulfonilureasRESUMEN
ATP-sensitive K+ (KATP) channels play a crucial role in coupling metabolic energy to the membrane potential of cells. We have isolated a cDNA encoding a novel member (uKATP-1) of the inward rectifier K+ channel family from a rat pancreatic islet cDNA library. Rat uKATP-1 is a 424-amino acid residue protein (M(r) = 47,960). Electrophysiological studies of uKATP-1 expressed in Xenopus laevis oocytes show that uKATP-1 is a weak rectifier and is blocked with Ba2+ ions. Single-channel patch clamp study of clonal human kidney epithelial cells (HEK293) transfected with uKATP-1 cDNA reveals that uKATP-1 closes in response to 1 mM ATP and has a single channel conductance of 70 +/- 2 picosiemens (n = 6), indicating that uKATP-1 is an ATP-sensitive inward rectifier K+ channel. In addition, uKATP-1 is activated by the KATP channel opener, diazoxide. RNA blot analysis shows that uKATP-1 mRNA is expressed ubiquitously in rat tissues, including pancreatic islets, pituitary, skeletal muscle, and heart, suggesting that uKATP-1 may play a physiological role as a link between the metabolic state and membrane K+ permeability of cells in almost every normal tissue. Since uKATP-1 shares only 43-46% amino acid identity with members of previously reported inward rectifier K+ channel subfamilies, including ROMK1, IRK1, GIRK1, and cKATP-1, uKATP-1 is not an isoform of these subfamilies and, therefore, represents a new subfamily of the inward rectifier K+ channel family having two transmembrane segments.
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Expresión Génica , Islotes Pancreáticos/metabolismo , Músculo Esquelético/metabolismo , Hipófisis/metabolismo , Canales de Potasio/biosíntesis , Transportadoras de Casetes de Unión a ATP , Adenosina Trifosfato/metabolismo , Secuencia de Aminoácidos , Animales , Clonación Molecular , Femenino , Biblioteca de Genes , Canales KATP , Masculino , Potenciales de la Membrana , Datos de Secuencia Molecular , Miocardio/metabolismo , Oocitos/fisiología , Especificidad de Órganos , Ovario/metabolismo , Técnicas de Placa-Clamp , Canales de Potasio/fisiología , Canales de Potasio de Rectificación Interna , ARN Mensajero/análisis , ARN Mensajero/biosíntesis , Ratas , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/metabolismo , Homología de Secuencia de Aminoácido , Testículo/metabolismo , Xenopus laevisRESUMEN
A new method was developed for quantitating the serum and urinary levels of 1,5-anhydroglucitol (AG), a sensitive and informative marker of glycemic control. This method utilized a combination of ODS and pyranose oxidase-immobilized columns for HPLC, and monitored hydrogen peroxide production with an electrochemical detector. We applied this method to determine the serum and urinary AG levels in 15 patients with insulin-dependent diabetes mellitus (IDDM) as well as in control subjects. Baseline separation of AG from other sugars such as glucose and myoinositol was achieved. Quantitation of AG was achieved over the range from 0.2 ng to 0.3 micrograms based upon peak heights. The serum and urinary AG levels in the IDDM patients were 4.4 +/- 8.3 mg/l and 5.1 +/- 4.3 mg/day, respectively. We found that the urinary AG to serum AG ratio showed a linear correlation with the urinary glucose level in the IDDM patients (urinary glucose (y) vs. urinary AG to serum AG ratio (x): y = 9.071x-0.991; r = 0.968, P < 0.001). This method proved efficient and reliable for quantitating urinary AG. Since determination of both the AG and glucose levels in urine gives equivalent clinical information to the serum AG level, urinary monitoring could provide a valuable addition to the available methods for assessing the glycemic status of IDDM patients.
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Desoxiglucosa/sangre , Diabetes Mellitus Tipo 1/sangre , Adolescente , Adulto , Biomarcadores/sangre , Biomarcadores/orina , Niño , Preescolar , Cromatografía Líquida de Alta Presión/métodos , Desoxiglucosa/orina , Diabetes Mellitus Tipo 1/orina , Electroquímica/métodos , Femenino , Glucosuria , Humanos , Indicadores y Reactivos , Isomerismo , Masculino , Sensibilidad y EspecificidadRESUMEN
The effects of polyriboinosinic acid-polyribocytidylic acid (poly IC) on galactosamine-induced liver cell injury in rats were studied. Treatment of rats with D-galactosamine-HC1 (400 mg/kg) increased their serum glutamate-oxaloacetate transaminase (E.C.2.6.1.1, GOT) activities, indicating that hepatocyte injury was induced by galactosamine. Rapid and intense elevations of serum GOT activities were observed when galactosamine (200 mg/kg) was administered simultaneously with poly IC (10 mg/kg) but not with poly I or poly C. Acute increase in serum GOT activities caused by the simultaneous administration of poly IC and galactosamine was prevented by the simultaneous administration of uridine (1 g/kg), which is known to inhibit the early biochemical alterations caused by the amino sugar in the hepatocyte. These findings suggest that poly IC intensifies the hepatotoxic effect of galactosamine in rats. This poly IC-induced sensitization was inhibited by pretreatment with poly IC (10 mg/kg) itself, when injected 24 hr before the administration of the hepatotoxin together with poly IC.
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Galactosamina/toxicidad , Hígado/efectos de los fármacos , Poli I-C/farmacología , Animales , Relación Dosis-Respuesta a Droga , Sinergismo Farmacológico , Masculino , Poli C/farmacología , Poli I/farmacología , Ratas , Ratas Endogámicas , Factores de Tiempo , Uridina/farmacologíaRESUMEN
The antitumor activities of the cell wall skeleton (CWS) of Nocardia rubra were demonstrated for syngeneic fibrosarcoma (AMC-60) in ACI/N rats in regard to macrophage activation. In the 24-hr cytolytic test, activated macrophages which were fractionated from peritoneal exudate cells induced by i.p. injection of Nocardia CWS showed significant cytolytic activity for [125I]iododeoxyuridine-labeled tumor cells. Activated macrophages also strongly inhibited [3H]thymidine incorporation into the tumor cells during the 24-hr cytostatic test. When tumor cells were inoculated s.c. with activated macrophages in the Winn-type transfer assay, subsequent tumor growth was significantly inhibited. Repeated i.p. injection of the CWS seemed to enhance these antitumor activities of macrophages. The therapeutic effect of Nocardia CWS was assessed with the ascites tumor and with the solid tumor inoculated i.m. into the hind leg. In the former treatment, repeated i.p. injections completely prevented the accumulation of ascites fluid and resulted in prolongation of the survival period. The peritoneal macrophages harvested from these survivors had a strong cytolytic activity for tumor cells in the cytolytic test. In the latter treatment, repeated intratumoral injections inhibited the growth of primary tumor and prevented metastasis. Furthermore, peritoneal resident macrophages from these tumor-bearing rats treated intratumorally with the CWS were found to be cytolytic for tumor cells in the cytolytic test.