Effect of low intensity photobiomodulation associated with norbixin-based poly (hydroxybutyrate) membrane on post-tenotomy tendon repair. In vivo study.

PURPOSE: To evaluate the in vivo response of photobiomodulation therapy associated with norbixin-based poly(hydroxybutyrate) membrane (PHB) in tenotomized calcaneal tendon. METHODS: Thirty rats were randomly allocated to six groups (n=5 each): LED groups (L1, L2 and L3) and membrane + LED groups (ML1, ML2 and ML3). The right calcaneal tendons of all animals were sectioned transversely and were irradiated with LED daily, one hour after surgery every 24 hours, until the day of euthanasia. At the end of the experiments the tendons were removed for histological analysis. RESULTS: The histological analysis showed a significant reduction in inflammatory cells in the ML1, ML2 and ML3 groups (p=0.0056, p=0.0018 and p<0.0001, respectively) compared to those in the LED group. There was greater proliferation of fibroblasts in the ML1 (p<0.0001) and L3 (p<0.0001) groups. A higher concentration of type I collagen was also observed in the ML1 group (p=0.0043) replacing type III collagen. CONCLUSION: Photobiomodulation in association with norbixin-based PHB membrane led to control of the inflammatory process. However, it did not favor fibroblast proliferation and did not optimize type I collagen formation in the expected stage of the repair process.

Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats.

PURPOSE: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. METHODS: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. RESULTS: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). CONCLUSION: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.

Effect of low intensity photobiomodulation associated with norbixin-based poly (hydroxybutyrate) membrane on post-tenotomy tendon repair. In vivo study

Abstract Purpose: To evaluate the in vivo response of photobiomodulation therapy associated with norbixin-based poly(hydroxybutyrate) membrane (PHB) in tenotomized calcaneal tendon. Methods: Thirty rats were randomly allocated to six groups (n=5 each): LED groups (L1, L2 and L3) and membrane + LED groups (ML1, ML2 and ML3). The right calcaneal tendons of all animals were sectioned transversely and were irradiated with LED daily, one hour after surgery every 24 hours, until the day of euthanasia. At the end of the experiments the tendons were removed for histological analysis. Results: The histological analysis showed a significant reduction in inflammatory cells in the ML1, ML2 and ML3 groups (p=0.0056, p=0.0018 and p<0.0001, respectively) compared to those in the LED group. There was greater proliferation of fibroblasts in the ML1 (p<0.0001) and L3 (p<0.0001) groups. A higher concentration of type I collagen was also observed in the ML1 group (p=0.0043) replacing type III collagen. Conclusion: Photobiomodulation in association with norbixin-based PHB membrane led to control of the inflammatory process. However, it did not favor fibroblast proliferation and did not optimize type I collagen formation in the expected stage of the repair process.

Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats

Abstract Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.

Effects of Therapy with Light Emitting Diode (LED) in the Calcaneal Tendon Lesions of Rats: A Literature Review.

The objective of this review was to analyze original articles about the effects of therapy with LED in experimental models of calcaneal tendon lesions of rats. The search was performed in the period from February to May 2018, in the following electronic databases: MEDLINE, SciELO, and LILACS, besides the Google Scholar, using the descriptors "Achilles tendon", "Rats", "LED", "Tendinopathy", and "Low-level Light Therapy", as well as their matching parts in the Portuguese and Spanish languages, related to and in association with the relevant terms to the content sought. From the descriptors used 215 works were found. After application of eligibility criteria 8 works were selected, in which positive results were found after the application of the LED. Regarding the main results found with phototherapy, we observed a significant reduction in inflammation. Only one article mentioned little reduction of inflammation. In relation to the number of sessions, there was wide variation, with an average of approximately 5 sessions every 24 hours. Studies in this review pointed out, therefore, positive results in the repair of the calcaneal tendon after therapy with irradiation LED; however, carrying out more experimental studies that help the standardization of parameters to be used in this therapy for further clinical studies becomes necessary.

Fatores associados à queda de idosos que podem resultar em fratura de fêmur / Factors associated with the fall of elderly which may result in femoral fracture / Factores asociados con la caída de ancianos que pueden resultar en fractura de fémur

Objetivo: analisar os fatores associados a queda de idosos que podem resultar em fratura de fêmur. Método: estudo exploratório e descritivo com abordagem qualitativa, desenvolvido no Centro Integrado de Saúde do Uninovafapi, com 50 idosos. Os dados foram coletados por meio de entrevista, processados no Iramuteq e analisados pela classificação hierárquica descendente. Resultados: foram apresentados em cinco categorias, a saber: 1. Condição das residências dos idosos que facilitam as quedas; 2. Falta de adaptação das residências às necessidades dos idosos; 3. Condições irregulares do calçamento das ruas; 4. Condições irregulares das calçadas das casas e comércios; e 5. Hipertensão arterial e diabetes, como doenças crônicas associadas às quedas entre idosos. Conclusão: os fatores associados à queda de idosos tem relação com as condições das residências e as ruas da cidade, mas o que determina a gravidade desses acidentes são as doenças crônicas que o idoso possui. Há necessidade de melhorar a política pública de atenção ao idoso relacionada às quedas.(AU)

Objective: analyzing the factors associated with the fall of the elderly that may result in femur fractures. Method: an exploratory and a descriptive study of a qualitative approach developed at the Integrated Health Center Uninovafapi, with 50 seniors. Data were collected through interviews, processed in Iramuteq and analyzed through the descending hierarchical classification. Results: they were presented in five categories, namely: 1. Condition of the homes of the elderly that facilitate the falls; 2. Lack of adapting homes to the needs of the elderly; 3. Irregular conditions of paving of streets; 4. Irregular conditions of the sidewalks of homes and businesses; and 5. High blood pressure and diabetes, as chronic diseases associated with falls among the elderly. Conclusion: the factors associated with the fall of the elderly is related to the conditions of the homes and streets of the city, but what determines the severity of these accidents are the chronic diseases that old people have. There is a need to improve public policy of elderly care related to falls. (AU)

Objetivo: analizar los factores asociados con la caída de las personas mayores que pueden dar lugar a fracturas de fémur. Método: estudio exploratorio y descriptivo con enfoque cualitativo desarrollado en el Centro Integrado de Salud Uninovafapi, con 50 personas mayores. Los datos fueron recolectados a través de entrevistas, procesados en Iramuteq y analizados por la clasificación jerárquica descendente. Resultados: se presentaron en cinco categorías, a saber: 1. Condición de las viviendas de las personas mayores que facilitan las caídas; 2. La falta de adaptación de viviendas a las necesidades de los ancianos; 3. Condiciones de pavimento irregular de calles; 4. Condiciones irregulares de las aceras de las casas y negocios; y 5. La presión arterial alta y la diabetes como las enfermedades crónicas asociadas con caídas entre los ancianos. Conclusión: los factores asociados con la caída de personas mayores está relacionada con las condiciones de las viviendas y calles de la ciudad, pero lo que determina la gravedad de estos accidentes son las enfermedades crónicas que las personas mayores tienen. Hay una necesidad de mejorar las políticas públicas relacionadas con el cuidado de ancianos a las caídas.(AU)