RESUMEN
This study evaluated the toxicity of benzene, toluene, and xylenes (BTX), isolated and in binary mixtures to Mysidopsis juniae. The organisms were exposed to BTX, and combined effect patterns were predicted by applying the theoretical models of Concentration Addition and Independent Action. According to the LC50 of the isolated compounds, xylene (16.1 ± 2.4 mg L-1) was considered the most toxic, followed by toluene (38.0 ± 5.3 mg L-1) and, lastly, benzene (78.0 ± 2.9 mg L-1). The binary combinations showed deviations from additivity, with exposure to the xylene-benzene mixture presenting as antagonistic, while the xylene-toluene and toluene-benzene mixtures were better explained by a dose ratio deviation, with toluene being responsible for the antagonistic pattern. This study provides new insights into toxicity prediction of a BTX mixture, which adds value to the risk assessment procedure over evaluation of chemical hazards on a case-by-case basis.
