Synthesis of magnetic Fe3O4/graphene aerogel for the removal of 2,4-dichlorophenoxyacetic acid herbicide from water.

Graphene-based aerogels are among the lightest materials in the world and have been extensively studied for environmental remediation. In this work, an Fe3O4/graphene aerogel material was synthesized using the co-precipitation method. The prepared material was characterized using X-ray diffraction (XRD), scanning electron microscopy/X-ray energy dispersive spectroscopy (FESEM/EDX), infrared spectroscopy (FT-IR), and vibration sample magnetization (VSM). The results showed that the Fe3O4 nanoparticles with a particle size of less than 100 nm were well-distributed on the surface of the graphene aerogel. The prepared Fe3O4/graphene aerogel showed effective removal of 2,4-D herbicide from the aqueous solution with a maximal adsorption capacity of approximately 42.918 mg g-1. The adsorption isotherms and kinetics were investigated to study the adsorption behaviour of the resultant material. The saturation magnetism value of the aerogel was determined to be about 20.66 emu g-1, indicating that the adsorbent could be easily collected from the solution using an external magnet. These results implied that the prepared Fe3O4/graphene aerogel could be a promising adsorbent for the removal of 2,4-D herbicide from water.

Simple and Label-Free Detection of Carboxylesterase and Its Inhibitors Using a Liquid Crystal Droplet Sensing Platform.

In this study, we developed a liquid crystal (LC) droplet-based sensing platform for the detection of carboxylesterase (CES) and its inhibitors. The LC droplet patterns in contact with myristoylcholine chloride (Myr) exhibited dark cross appearances, corresponding to homeotropic anchoring of the LCs at the aqueous/LC interface. However, in the presence of CES, Myr was hydrolyzed; therefore, the optical images of the LC patterns changed to bright fan-shaped textures, corresponding to a planar orientation of LCs at the interface. In contrast, the presence of CES inhibitors, such as benzil, inhibits the hydrolysis of Myr; as a result, the LC patterns exhibit dark cross textures. This principle led to the development of an LC droplet-based sensing method with a detection limit of 2.8 U/L and 10 µM, for CES detection and its inhibitor, respectively. The developed biosensor not only enables simple and label-free detection of CES but also shows high promise for the detection of CES inhibitors.

Ultrasensitive colorimetric detection of amoxicillin based on Tris-HCl-induced aggregation of gold nanoparticles.

An ultrasensitive colorimetric aptasensor for the detection of amoxicillin (AMO) based on the Tris-HCl buffer-induced aggregation of gold nanoparticles (AuNPs) was developed. The AuNPs were aggregated by the addition of Tris-HCl buffer. The adsorption of the aptamer on the AuNP surface increased its negative charge density, leading to the enhancement of the electrostatic repulsion between the nanoparticles, thus protecting AuNPs from aggregation in the Tris-HCl buffer. However, the specific binding of the aptamer with AMO induced conformational changes in the aptamer, which reduced the adsorption of the aptamer on the AuNP surface, diminishing the protective effect of the aptamer. This resulted in the aggregation of AuNPs by Tris-HCl buffer, and consequently, color change of the solution containing AuNPs from red to blue. Under optimized conditions, a linear relationship between the absorbance ratio variation (ΔA680/A520) and the AMO concentration was observed in the concentration range of 0.1-125 nM, with a detection limit of 67 pM. The developed biosensor exhibited high selectivity toward AMO. Moreover, this strategy was successfully applied to the detection of AMO in lake water samples. Thus, the present aptasensor is a promising alternative for the simple and ultrasensitive detection of AMO in the environment.

A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles.

Here, we designed a simple, rapid, and ultrasensitive colorimetric aptasensor for detecting anatoxin-a (ATX-a). The sensor employs a DNA aptamer as the sensing element and gold nanoparticles (AuNPs) as probes. Adsorption of the aptamer onto the AuNP surface can protect AuNPs from aggregation in NaCl solution, thus maintaining their dispersion state. In the presence of ATX-a, the specific binding of the aptamer with ATX-a results in a conformational change in the aptamer, which facilitates AuNP aggregation and, consequently, a color change of AuNPs from red to blue in NaCl solution. This color variation is directly associated with ATX-a concentration and can be easily measured using a UV/Vis spectrophotometer. The absorbance variation is linearly proportional to ATX-a concentration across the concentration range of 10 pM to 200 nM, with a detection limit of 4.45 pM and high selectivity against other interferents. This strategy was successfully applied to the detection of ATX-a in lake water samples. Thus, the present aptasensor is a promising alternative method for the rapid detection of ATX-a in the environment.

A Label-Free Liquid Crystal Biosensor Based on Specific DNA Aptamer Probes for Sensitive Detection of Amoxicillin Antibiotic.

We developed a liquid crystal (LC) aptamer biosensor for the sensitive detection of amoxicillin (AMX). The AMX aptamer was immobilized onto the surface of a glass slide modified with a mixed self-assembled layer of dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP) and (3-aminopropyl) triethoxysilane (APTES). The long alkyl chains of DMOAP maintained the LC molecules in a homeotropic orientation and induced a dark optical appearance under a polarized light microscope (POM). In the presence of AMX, the specific binding of the aptamer and AMX molecules induced a conformational change in the aptamers, leading to the disruption of the homeotropic orientation of LCs, resulting in a bright optical appearance. The developed aptasensor showed high specificity and a low detection limit of 3.5 nM. Moreover, the potential application of the developed aptasensor for the detection of AMX in environmental samples was also demonstrated. Therefore, the proposed aptasensor is a promising platform for simple, rapid, and label-free monitoring of AMX in an actual water environment with high selectivity and sensitivity.

A Cationic Surfactant-Decorated Liquid Crystal-Based Aptasensor for Label-Free Detection of Malathion Pesticides in Environmental Samples.

We report a liquid crystal (LC)-based aptasensor for the detection of malathion using a cationic surfactant-decorated LC interface. In this method, LCs displayed dark optical images when in contact with aqueous cetyltrimethylammonium bromide (CTAB) solution due to the formation of a self-assembled CTAB monolayer at the aqueous/LC interface, which induced the homeotropic orientation of LCs. With the addition of malathion aptamer, the homeotropic orientation of LCs changed to a planar one due to the interactions between CTAB and the aptamer, resulting in a bright optical image. In the presence of malathion, the formation of aptamer-malathion complexes caused a conformational change of the aptamers, thereby weakening the interactions between CTAB and the aptamers. Therefore, CTAB is free to induce a homeotropic ordering of the LCs, which corresponds to a dark optical image. The developed sensor exhibited high specificity for malathion determination and a low detection limit of 0.465 nM was achieved. Moreover, the proposed biosensor was successfully applied to detect malathion in tap water, river water, and apple samples. The proposed LC-based aptasensor is a simple, rapid, and convenient platform for label-free monitoring of malathion in environmental samples.

An acetylcholinesterase-based biosensor for the detection of pesticides using liquid crystals confined in microcapillaries.

Here, we demonstrate a capillary-sensing platform based on liquid crystals (LCs) confined in microcapillaries for simple and sensitive detection of acetylcholinesterase (AChE) and its inhibitors. LC droplets were formed through sequential injection of LCs and an aqueous solution into trichloro(octyl)silane (OTS)-treated microcapillaries. When the confined LC droplets make contact with a cationic surfactant solution, myristoylcholine chloride (Myr), the formation of a Myr monolayer at the aqueous/LC interface induces a horizontal orientation of the LCs at the interface along the microcapillary, producing an optical LC droplet texture of a four-petal shape. On the other hand, AChE can catalyze the hydrolysis of Myr into choline and myristic acid. The hydrolyzed Myr is unable to form a monolayer at the aqueous/LC interface, and therefore the confined LC droplets exhibit two bright-lined optical images when in contact with the pre-incubated mixture of Myr and AChE, corresponding to the homeotropic orientation of LCs at the interface. However, in the presence of AChE-inhibiting pesticides, such as fenobucarb and malathion, the activity of AChE is inhibited, and thus, the enzymatic hydrolysis of Myr cannot occur. As a result, the confined LC droplets present the four petal-shaped optical images when in contact with the pre-incubated mixture of Myr, AChE, and pesticides. Based on this principle, an LC-based microcapillary sensor was developed and utilized for the detection of pesticides. Using this sensing platform, fenobucarb and malathion were detected at limits of 5 pg/mL and 2.5 pg/mL, respectively. Moreover, the proposed biosensor was successfully applied to the determination of pesticides in real river water. Therefore, this LC-based microcapillary sensor is a promising platform for simple, rapid, and label-free detection of pesticides with very high sensitivity.

Label-free liquid crystal-based biosensor for detection of dopamine using DNA aptamer as a recognition probe.

We present a label-free liquid crystal-based biosensor for the detection of dopamine (DA) in aqueous solutions using dopamine-binding aptamers (DBA) as recognition elements. In this system, the dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP) self-assembled monolayers immobilized on glass slides support the long alkyl chains that keep the liquid crystal (LC) molecules in a homeotropic orientation. Glutaraldehyde (GA) is used as a cross-linker to immobilize DBA onto the surface of glass slides. The specific binding of DA and DBA disrupts the homeotropic orientation of LCs, thereby inducing a change in the orientation from homeotropic to a random alignment. This orientation change can be converted and visualized simply as a transition from a dark optical LC image to a brighter image under a polarized optical microscope (POM), enabling the detection of DA. The developed LC-based aptasensor shows a good linear optical response towards DA in the very wide range of 1 pM-10 µM (0.19 pg/mL to 1.9 µg/mL) and has a very low detection limit of 10 pM (∼1.9 pg/mL). The biosensor also exhibited satisfactory selectivity and could be successfully applied to detect DA in human urine. The proposed LC-based aptamer sensing method offers a simple, rapid, highly sensitive and selective, and a label-free method for the analysis of DA in real clinical samples.

Synthesis and Irreversible Thermochromic Sensor Applications of Manganese Violet.

An irreversible thermochromic material based on manganese violet (MnNH4P2O7) is synthesized. The crystal phase, chemical composition, and morphology of the synthesized material are analyzed using X-ray diffraction, scanning electron microscopy coupled with energy-dispersive X-ray spectrometry, and Fourier-transform infrared spectroscopy. The absorption spectra of the synthesized material are obtained using a UV-Vis spectrometer, and the thermochromism exhibited by the powdered samples at high temperatures is also investigated. The as-synthesized manganese violet pigment consists of pure α-MnNH4P2O7 phase. In addition, the synthesized pigment largely consists of hexagonal crystals with a diameter of hundreds of nanometers. On heating, the pigment simultaneously loses H2O and NH3 in two successive steps at approximately 330⁻434.4 °C and 434.4⁻527 °C, which correspond to the formation of an intermediate phase and of Mn2P4O12, respectively. An overall mass loss of 14.22% is observed, which is consistent with the expected 13.79%. An irreversible color change from violet to white is observed after exposure of the synthesized manganese violet pigment at 400 °C for 30 min. This is attributed to the oxidation of ammonia to hydroxylamine, which then decomposes to nitrogen and water, or alternatively to the direct oxidation of ammonia to nitrogen. Furthermore, we demonstrate the potential application of synthesized manganese violet in the production of irreversible thermochromic paint by mixing with potassium silicate solution as a binder and deionized water as a solvent at a specific ratio. The thermochromic paint is then applied in fabrication of irreversible thermochromic sensors by coating it onto a steel plate surface. Finally, we show that manganese violet-based irreversible thermochromic sensors are able to detect temperatures around 400 °C by changing color from violet to white/milky.

Synthesis and Thermochromic Properties of Cr-Doped Al2O3 for a Reversible Thermochromic Sensor.

An inorganic thermochromic material based on Cr-doped Al2O3 is synthesized using a solid-state method. The crystal structure, chemical composition, and morphology of the synthesized material are analyzed using X-ray diffraction, scanning electron microscopy coupled with an energy-dispersive X-ray spectrometer, and Fourier transform infrared (FT-IR) spectroscopy. The color performances of the synthesized material are analyzed using a UV-VIS spectrometer. Finally, the thermochromism exhibited by the powdered samples at high temperatures is investigated. The material exhibits exceptional thermochromic property, transitioning from pink to gray or green in a temperature range of 25-600 °C. The change in color is reversible and is dependent on the surrounding temperature and chromium concentration; however, it is independent of the exposure time. This novel property of Cr-doped Al2O3 can be potentially employed in reversible thermochromic sensors that could be used not only for warning users of damage due to overheating when the environmental temperature exceeds certain limits, but also for detecting and monitoring the temperature of various devices, such as aeronautical engine components, hotplates, and furnaces.

Hydrogel Encapsulation of Cells in Core-Shell Microcapsules for Cell Delivery.

A newly designed 3D core-shell microcapsule structure composed of a cell-containing liquid core and an alginate hydrogel shell is fabricated using a coaxial dual-nozzle electrospinning system. Spherical alginate microcapsules are successfully generated with a core-shell structure and less than 300 µm in average diameter using this system. The thickness of the core and shell can be easily controlled by manipulating the core and shell flow rates. Cells encapsulated in core-shell microcapsules demonstrate better cell encapsulation and immune protection than those encapsulated in microbeads. The observation of a high percentage of live cells (≈80%) after encapsulation demonstrates that the voltage applied for generation of microcapsules does not significantly affect the viability of encapsulated cells. The viability of encapsulated cells does not change even after 3 d in culture, which suggests that the core-shell structure with culture medium in the core can maintain high cell survival by providing nutrients and oxygen to all cells. This newly designed core-shell structure can be extended to use in multifunctional platforms not only for delivery of cells but also for factor delivery, imaging, or diagnosis by loading other components in the core or shell.