Case of Esophageal Actinomycosis Occurred after Endoscopic Mucosal Resection for Subepithelial Tumor.

Esophageal actinomycosis is a rare, chronic granulomatous disease caused by Actinomyces species. Endoscopy and biopsy are essential for making a diagnosis. This paper reports a case of esophageal actinomycosis that developed after an endoscopic mucosal resection (EMR) for a subepithelial tumor (SET). A 74-year-old male patient had a 3 cm flat, smooth elevation in the esophagus without symptoms. The SET was partially resected, and histology revealed "nonspecific degenerated mesenchymal tissue". Three months later, the patient exhibited a persistently large ulceration at the EMR site, and a biopsy revealed actinomycosis. CT of the chest and abdomen revealed no abnormal findings. Ampicillin treatment was administered for six months, and the ulceration on the esophageal SET improved.

Structural evolution of in-plane hybrid graphene/hexagonal boron nitride heterostructure upon heating.

In-plane hybrid graphene/hexagonal boron nitride (h-BN) heterostructure (graphene/hBN/graphene) is studied via molecular dynamics simulation. The initial configuration (6400-atom graphene/6200-atom h-BN/6400-atom graphene) is heated up from 50 K to 7500 K via Tersoff potential. To study the structural evolution, some thermal dynamics quantities are calculated such as the coordination number, the total energy per atom, the heat capacity, the angular distribution, and the distribution of rings. Some main results are calculated and presented as follows: i) The sudden increase of total energy per atom at the melting point (5500 K) exhibits the first order phase transition from the crystalline state to a liquid state of the hybrid graphene/h-BN/graphene heterostructure; ii) The heat capacity shows two peaks. The first peak (at 5500 K) represents the phase transition from the crystalline to a liquid states while the second one (at 6300 K) represents the formation of gaseous atoms of B and N in the h-BN sheet; iii) The coordination number of three decreases dramatically at temperature of 5500 K (about 10% lefts for each type of atoms) leading to the formation of the first peak in the graph of the heat capacity. The coordination number of zero for B and N in the h-BN layer increases significantly (over 55%) at 6300 K causing the formation of the second peak in the graph of the heat capacity; iv) The influence of the relative number of atoms of h-BN to graphene in the hybrid graphene/h-BN/graphene heterostructure on the structural evolution upon heating is considered as follows. The number of atoms in the graphene sheets remains constant (6400 atoms per sheet) while the one of the h-BN sheet varies in size (780, 1560, 3120, 4680, 5490, 5880, 6080, and 6200 atoms). The results show that although the phase transition is still the first order type, the phase transition temperature decreases as the size of the h-BN layer in the hybrid heterostructure increases.

Compression-induced hexa-to-tetra phase transition of confined germanene.

Via molecular dynamics (MD) simulations we find the existence of the new allotrope of two-dimensional (2D) germanene, i.e. 2D tetra-germanene (tetra-Ge) which contains entirely tetragons. We compress 2D hexa-germanene (hexa-Ge) step by step over a broad density range at constant temperature and hexa-tetra Ge phase transition occurs. We find that the compression of hexa-Ge at 2000 K (not far above the melting point of hexa-Ge) leads to the formation of tetra-Ge with the highest quality. Atomic structure of the obtained tetra-Ge at 300 K is analyzed in details. Although fraction of tetragons in the tetra-Ge is very high (larger than 0.99), some defects are found in addition to the skew tetragons. Due to containing almost entirely tetragons, tetra-Ge may exhibit new behaviors unlike those of the hexa-Ge. Subsequent studies in this direction for 2D tetra-Ge. In addition, first-principles calculations under density functional theory confirm the existence of stable tetra-Ge.

Efficient and recyclable Nd3+-doped CoFe2O4 for boosted visible light-driven photocatalytic degradation of Rhodamine B dye.

Rare earth metal doping spinel ferrites offer excellent electronic, magnetic, and photocatalytic properties, but they have not been well explored for environmental mitigation. Herein, we report the facile fabrication of novel CoNd x Fe2-x O4 (x = 0-0.05) photocatalysts based on Nd3+ incorporated into CoFe2O4 for the degradation of Rhodamine B under visible light irradiation. The Nd3+ dopant considerably increased the specific surface area (35 m2 g-1) and enhanced the degradation performance (94.7%) of CoNd x Fe2-x O4 catalysts. Nd3+-doped CoFe2O4 played a role in the formation of radicals, including ˙OH, h+, and ˙O2 -. With high recyclability and performance, CoNd0.05Fe1.95O4 nanoparticles can be efficient and reusable photocatalysts for degrading organic dyes, including Rhodamine B from wastewaters.

Toward enhanced visible-light photocatalytic dye degradation and reusability of La3+ substituted ZnFe2O4 nanostructures.

The present work focused on the synthesis of novel ZnLaxFe2-xO4 catalysts (x = 0, 0.01, 0.03, 0.05) and their utilization for the photocatalytic degradation of Rhodamine B dye. Structurally, the band gap energy of the catalysts tended to decrease (1.94-1.70 eV) with increasing the amount of La3+ dopant. ZnLa0.05Fe1.95O4 had an average particle size (40 nm), high surface area (41.07 m2 g-1) and large pore volume (0.186 cm3 g-1). Moreover, the effect of doping ratio, reaction time, H2O2 concentration, catalyst loading on the treatment performance of La3+ substituted ZnFe2O4 nanocomposites was investigated. ZnLa0.05Fe1.95O4/H2O2 system exhibited the highest degradation efficiency of 99.5% and nonlinear pseudo first-order kinetic reaction rate (14.8 × 10-3 min-1) in the presence of visible light irradiation. The key role of reactive oxygen species involving •O2- and •OH radicals was well explained through the scavenger study. A plausible mechanism of the degradation of Rhodamine B dye was also proposed. Due to two advantageous points including high recyclability (up to 4 cycles) and stability, La3+ substituted ZnFe2O4 nanocomposites can be an effective and competitive catalyst for the visible light-driven photodegradation of toxic dyes in the real wastewaters.

Coralline photosynthetic physiology across a steep light gradient.

Coralline algae (CA) are globally distributed and fulfil many important roles within coastal ecosystems. In this study, photosynthetically active radiation (PAR) measured for 616 days at 2 and 10 m in a temperate subtidal kelp forest in southern New Zealand provided context to photosynthesis vs. irradiance relationships for, and pigment concentrations of, an articulated coralline alga, Arthrocardia sp. and a crustose coralline species assemblage within the Hapalidiales order. The maximum photosynthetic rate Pmax of the Arthrocardia sp. (20.38 ± 2.38 µmol O2. gDW-1 h-1) was significantly higher than the Pmax of crustose coralline spp. (3.72 ± 0.74 µmol O2. gDW-1 h-1) at the same 2 m stratum. Pigment concentration of Arthrocardia sp. was significantly higher than that of crustose coralline spp. at the same depth, while pigment concentration of crustose coralline spp. at 2 and 10 m were not significantly affected by depth. The photosynthetic characteristics of these coralline algae represent a shade acclimated organism with low saturation irradiance (all Ek < 100 µmol photons m-2 s-1). Despite sevenfold difference in average daily dose between 2 and 10 m there was no significant effect of depth on the photosynthetic performance of crustose coralline algae measured. The lack of evidence for acclimation to low light could be because periods of clear water provide enough light to maintain photosynthesis, lower energetic requirements of species found at depth or constraints on the synthesis of photosynthetic pigments at greater depth.

Enhanced Photocatalytic Activity of Spherical Nd3+ Substituted ZnFe2O4 Nanoparticles.

In this study, nanocrystalline ZnNdxFe2-xO4 ferrites with x = 0.0, 0.01, 0.03 and 0.05 were fabricated and used as a catalyst for dye removal potential. The effect of Nd3+ ions substitution on the structural, optical and photo-Fenton activity of ZnNdxFe2-xO4 has been investigated. The addition of Nd3+ ions caused a decrease in the grain size of ferrites, the reduction of the optical bandgap energies and thus could be well exploited for the catalytic study. The photocatalytic activity of the ferrite samples was evaluated by the degradation of Rhodamine B (RhB) in the presence of H2O2 under visible light radiation. The results indicated that the ZnNdxFe2-xO4 samples exhibited higher removal efficiencies than the pure ZnFe2O4 ferrites. The highest degradation efficiency was 98.00%, attained after 210 min using the ZnNd0.03Fe1.97O4 sample. The enhanced photocatalytic activity of the ZnFe2O4 doped with Nd3+ is explained due to the efficient separation mechanism of photoinduced electron and holes. The effect of various factors (H2O2 oxidant concentration and catalyst loading) on the degradation of RhB dye was clarified.

Corrigendum: The Hsp90 Inhibitor, Monorden, Is a Promising Lead Compound for the Development of Novel Fungicides.

[This corrects the article DOI: 10.3389/fpls.2020.00371.].

A random parameter logistic model of fatigue-related motorcycle crash involvement in Hanoi, Vietnam.

Since motorcycle taxi drivers often work long hours, fatigue would affect their riding abilities, impacting crash risks. However, there is limited understanding about motorcycle taxi drivers' fatigue-related crashes. This study investigates self-reported fatigue-related crashes among motorcycle taxi drivers in Hanoi, Vietnam. Results from a survey showed that approximately 16% of the motorcycle taxi drivers reported fatigue-related crash involvement. It was also found that nearly 37% of all crashes reported by motorcycle taxi drivers were related to fatigue while riding a motorcycle taxi. Results of the heterogeneity-in-means random parameter logistic model suggested that working fulltime, more delivery trips, and overweight conditions were associated with increased likelihoods of fatigue-related crash involvement. Hybrid taxi drivers, who operate as either traditional or ride-hailing taxi drivers at different times, and most ride-hailing taxi drivers had a reduced likelihood of fatigue-related crash involvement when compared to traditional taxi drivers. Overall, this study has revealed a significant issue of fatigue-related crashes among motorcycle taxi drivers. Immediate interventions via publicity or educational campaigns should be considered by authorities to address this important issue. Ride-hailing companies should contribute by sending warnings of excessive riding hours to ride-hailing taxi drivers.

The Hsp90 Inhibitor, Monorden, Is a Promising Lead Compound for the Development of Novel Fungicides.

Endophytic fungi are great resources for the identification of useful natural products such as antimicrobial agents. In this study, we performed the antifungal screening of various plant endophytic fungi against the dollar spot pathogen Sclerotinia homoeocarpa and finally selected Humicola sp. JS-0112 as a potential biocontrol agent. The bioactive compound produced by the strain JS-0112 was identified as monorden known as an inhibitor of heat shock protein 90 (Hsp90). Monorden exhibited strong antagonistic activity against most tested plant pathogenic fungi particularly against tree pathogens and oomycetes with the minimum inhibitory concentration values less than 2.5 µg mL-1. Extensive in planta assays revealed that monorden effectively suppressed the development of several important plant diseases such as rice blast, rice sheath blight, wheat leaf rust, creeping bentgrass dollar spot, and cucumber damping-off. Especially, it showed much stronger disease control efficacy against cucumber damping-off than a synthetic fungicide chlorothalonil. Subsequent molecular genetic analysis of fission yeast and Fusarium graminearum suggested that Hsp90 is a major inhibitory target of monorden, and sequence variation among fungal Hsp90 is a determinant for the dissimilar monorden sensitivity of fungi. This is the first report dealing with the disease control efficacy and antifungal mechanism of monorden against fungal plant diseases and we believe that monorden can be used as a lead molecule for developing novel fungicides with new action mechanism for the control of plant diseases caused by fungi and oomycetes.

Autophagy: A Novel Mechanism Involved in the Anti-Inflammatory Abilities of Probiotics.

BACKGROUND/AIMS: Deregulation of the complex interaction among host genetics, gut microbiota and environmental factors on one hand and aberrant immune responses on the other hand, are known to be associated with the development of inflammatory bowel disease. Recent studies provided strong evidence that autophagy plays a key role in the etiology of Crohn's disease (CD). Probiotics may exhibit many therapeutic properties, including anti-inflammatory abilities. While successful results have been obtained in ulcerative colitis patients, probiotics remain inefficient in CD for unknown reason. It remains therefore important to better understand their molecular mechanisms of action. METHODS: The activation of autophagy was examined by stimulating bone marrow-derived dendritic cells by the bacteria, followed by confocal microscopy and western blot analysis. The impact of blocking in vitro autophagy was performed in peripheral blood mononuclear cells using 3-methyl adenine or bafilomycin followed by cytokine secretion measurement by ELISA. The role of autophagy in the anti-inflammatory capacities of the bacterial strains was evaluated in vivo using an acute trinitrobenzene sulfonic acid-induced murine model of colitis. The impact of BMDC was evaluated by adoptive transfer, notably using bone marrow cells derived from autophagy-related 16-like 1-deficient mice. RESULTS: We showed that selected lactobacilli and bifidobacteria are able to induce autophagy activation in BMDCs. Blocking in vitro autophagy abolished the capacity of the strains to induce the release of the anti-inflammatory cytokine interleukin-10, while it exacerbated the secretion of the pro-inflammatory cytokine interleukin-1ß. We confirmed in the TNBS-induced mouse model of colitis that autophagy is involved in the protective capacity of these selected strains, and showed that dendritic cells are involved in this process. CONCLUSION: We propose autophagy as a novel mechanism involved in the regulatory capacities of probiotics.

Mobile phone related crashes among motorcycle taxi drivers.

In many countries, motorcycle taxis remain an important mode of travel due to their fast, flexible, and inexpensive service. The recent advent of ride-hailing services has led to dramatic growth in the fleet of motorcycle taxis and additional types of motorcycle taxi drivers. Furthermore, mobile phone use while riding a motorcycle is an emerging safety issue, particularly among ride-hailing motorcycle taxi drivers. This paper investigates mobile phone use while riding, crashes and mobile phone related crashes among ride-hailing, traditional, and hybrid motorcycle taxi drivers, using data from a survey in Hanoi, Vietnam. Results show that ride-hailing motorcycle taxi drivers had the highest prevalence of mobile phone use while riding a motorcycle taxi (95.3%), followed by hybrid (88.6%) and traditional taxi drivers (64%). Approximately 32.6%, 19.3%, and 9.7% of motorcycle taxi drivers reported being involved in a crash, injury crash, and mobile phone related crash respectively. Mobile phone related crashes represent 20.5% of all reported crashes. Logistic and negative binomial regression were used to explore factors influencing mobile phone use while riding and crash frequencies. Regression results indicate that ride-hailing taxi drivers were more likely to be involved in a mobile phone related crash. Delivery trips were found to be associated with increases in crashes whereas passenger trips were found to be associated with decreases in crashes. Policy implications are also discussed.

Pedestrian overpass use and its relationships with digital and social distractions, and overpass characteristics.

Pedestrian deaths and injuries are a major health issue in both developed and developing countries. In Vietnam, pedestrians account for about 10-11% of all road traffic deaths, while their travel distance contributes to approximately 2.4% of the total distance travelled by all modes. This paper aims to explore the use of pedestrian overpasses and identify influencing factors, particularly with regards to social and digital distractions, and overpass characteristics. An observational survey was conducted in Hanoi, Vietnam, in March 2017 at ten pedestrian overpasses. Behaviours of 608 pedestrians, including those who used an overpass to cross and those who illegally crossed, were observed. The rates of overpass usage varied significantly, between 35.9% and 96.5%. Modelling results suggest that pedestrians tended to compensate for the risks of illegal crossing by forming group and avoiding digital and social distractions (i.e., calling, operating a mobile phone's screen, listening to music, or talking to other pedestrians while crossing). In addition, overpass usage decreased with taller overpasses, but increased with wider overpasses. Effects of gender, weather, and illegal crossing speed on overpass use were also discussed.

Mobile phone use while riding a motorcycle and crashes among university students.

OBJECTIVE: Motorcycle crashes are a significant road safety challenge, particularly in many low- and middle-income countries where motorcycles represent the vast majority of their vehicle fleet. Though risky riding behaviors, such as speeding and riding under the influence of alcohol, have been identified as important contributors to motorcycle crashes, little is understood about the effect of using a mobile phone while riding on motorcycle crash involvement. This article investigates crash involvement among motorcycle riders with risky riding behaviors, particularly using a mobile phone while riding. METHODS: Data were obtained from an online survey of university students' risky riding behaviors in Vietnam administered between March and May 2016 (n = 665). RESULTS: Results show that 40% of motorcycle riders reported to have experienced a crash/fall and nearly 24% of motorcycle riders indicated that they had been injured in a crash/fall. Effects of mobile phone use while riding on safety of motorcycle riders are highlighted. Specifically, more frequent use of a mobile phone for texting or searching for information while riding is associated with a higher chance of being involved in a crash/fall. The results also show that drink riding is associated with a higher chance of being injured. CONCLUSIONS: Overall this article reveals significant safety issues of using a mobile phone while riding a motorcycle, providing valuable insight for designing education and publicity campaigns.

Crohn's Disease-Associated Adherent-Invasive Escherichia coli Manipulate Host Autophagy by Impairing SUMOylation.

The intestinal mucosa of Crohn's disease (CD) patients is abnormally colonized with adherent-invasive Escherichia coli (AIEC) that are able to adhere to and to invade intestinal epithelial cells (IECs), to survive in macrophages, and to induce a pro-inflammatory response. AIEC persist in the intestine, and induce inflammation in CEABAC10 transgenic mice expressing human CAECAM6, the receptor for AIEC. SUMOylation is a eukaryotic-reversible post-translational modification, in which SUMO, an ubiquitin-like polypeptide, is covalently linked to target proteins. Here, we investigated the role of SUMOylation in host responses to AIEC infection. We found that infection with the AIEC LF82 reference strain markedly decreased the levels of SUMO-conjugated proteins in human intestinal epithelial T84 cells. This was also observed in IECs from LF82-infected CEABAC10 transgenic mice. LF82-induced deSUMOylation in IECs was due in part to increased level of microRNA (miR)-18, which targets PIAS3 mRNA encoding a protein involved in SUMOylation. Over-expression of SUMOs in T84 cells induced autophagy, leading to a significant decrease in the number of intracellular LF82. Consistently, a decreased expression of UBC9, a protein necessary for SUMOylation, was accompanied with a decrease of LF82-induced autophagy, increasing bacterial intracellular proliferation and inflammation. Finally, the inhibition of miR-18 significantly decreased the number of intracellular LF82. In conclusion, our results suggest that AIEC inhibits the autophagy response to replicate intracellularly by manipulating host SUMOylation.

Correlations between mobile phone use and other risky behaviours while riding a motorcycle.

Motorcyclist safety is a major concern in many developing countries. Understanding motorcycle riders' risky behaviours, particularly among the younger population, is essential to developing effective interventions. This paper explores the correlations between mobile phone use while riding and other risky riding behaviours as well as the relationships between perceived risks and risky riding behaviours, using an online survey of university students in Vietnam. Results show that calling while riding a motorcycle had the highest prevalence (74%) while reckless overtaking had the lowest prevalence (33.2%). Survey participants who indicated that they had the behaviours of reckless overtaking or riding on sidewalks were around twice as likely to call, text, or search for information while riding. In addition, those who admitted that they rode a motorcycle while under the influence of alcohol were nearly twice as likely to call or text while riding. The results also show that perceived crash risks reduced the likelihood of risky riding behaviours, including calling, texting, searching for information, speeding, running red lights, riding on the wrong side of a road, and riding on sidewalks. A more coordinated approach to enforcement is needed to help reduce the prevalence of multiple risk taking behaviours among motorcyclists.

Who's calling? Social networks and mobile phone use among motorcyclists.

Mobile phone use while riding a motorcycle poses a key safety risk, particularly among younger people who have been found to be more susceptible to distracted driving. While previous research has examined the influence of social networks on mobile phone use while driving a car, no research has explored this association in the context of motorcycle use. Using a survey of university students in Vietnam, this research explores the association between social networks and mobile phone use among motorcyclists and the links this has to reported crashes/falls. Results show that the majority of students are most likely to use a mobile phone to communicate with a friend while riding, either through talking (56.5%) or text messaging (62.0%). However, respondents who frequently talk to a girlfriend/boyfriend or spouse while riding were more likely to experience a crash/fall than those who frequently talk with others while riding (e.g. parent, brother/sister). In addition, those who frequently text message a friend while riding were more likely to experience a crash/fall than those who frequently text message others while riding. The results highlight a clear association between social networks and mobile phone use while riding a motorcycle. Developing a culture of societal norms, where mobile phone use while riding a motorcycle is considered socially unacceptable, will help to reduce the prevalence and ultimate crash risk associated with mobile phone use while riding.

Calling, texting, and searching for information while riding a motorcycle: A study of university students in Vietnam.

OBJECTIVE: The objective of this study was to investigate the prevalence of calling, texting, and searching for information while riding a motorcycle among university students and the influences of sociodemographic characteristics, social norms, and risk perceptions on these behaviors. METHODS: Students at 2 university campuses in Hanoi and Ho Chi Minh City, the 2 largest cities in Vietnam, were invited to participate in an anonymous online survey. Data collection was conducted during March and May 2016. RESULTS: There were 741 respondents, of whom nearly 90% of students (665) were motorcycle riders. Overall prevalence of mobile phone use while riding is 80.9% (95% confidence interval [CI], 77.9-83.9%) with calling having a higher level of prevalence than texting or searching for information while riding: 74% (95% CI, 70.7-77.3%) vs. 51.7% (95% CI, 47.9-55.5%) and 49.9% (95% CI, 46.1-53.7%), respectively. Random parameter ordered probit modeling results indicate that mobile phone use while riding is associated with gender, motorcycle license duration, perceived crash risk, perceived risk of mobile phone snatching, and perceptions of friends' mobile phone use while riding. CONCLUSIONS: Mobile phone use while riding a motorcycle is highly prevalent among university students. Educational programs should focus on the crash and economic risk of all types of mobile phone use while riding, including calling, texting, and searching for information. In addition, they should consider targeting the influence of social norms and peers on mobile phone use while riding.

Mobile phone use among motorcyclists and electric bike riders: A case study of Hanoi, Vietnam.

Motorcyclist injuries and fatalities are a major concern of many developing countries. In Vietnam, motorcycles are involved in more than 70% of all road traffic crashes. This paper aims to explore the prevalence and factors associated with mobile phone use among motorcyclists and electric bike riders, using a case study of Hanoi, Vietnam. A cross-sectional observation survey was undertaken at 12 sites, in which each site was surveyed during a two-hour peak period from 16:30 to 18:30 for two weekdays and one weekend day. A total of 26,360 riders were observed, consisting of 24,759 motorcyclists and 1601 electric bike riders. The overall prevalence of mobile phone use while riding was 8.4% (95% CI: 8.06-8.74%) with calling having higher prevalence than screen operation: 4.64% (95% CI: 4.39-4.90%) vs. 3.76% (95% CI: 3.52-3.99%) respectively. Moreover, the prevalence of mobile phone use was higher among motorcyclists than electric bike riders: 8.66% (95%CI: 8.30-9.01%) vs. 4.43% (95% CI: 3.40-5.47%) respectively. Logistic regression analyses revealed that mobile phone use while riding was associated with vehicle type, age, gender, riding alone, weather, day of week, proximity to city centre, number of lanes, separate car lanes, red traffic light duration, and police presence. Combining greater enforcement of existing legislations with extensive education and publicity programs is recommended to reduce potential deaths and injuries related to the use of mobile phones while riding.

Functional TNFα gene silencing mediated by polyethyleneimine/TNFα siRNA nanocomplexes in inflamed colon.

During inflammatory bowel disease, TNFα is the major pro-inflammatory cytokine mainly secreted from macrophages and dendritic cells. Here, we have demonstrated that TNFα siRNA/polyethyleneimine loaded into polylactide at an optimal concentration of 20 g/L nanoparticles covered with polyvinyl alcohol are efficiently taken up by inflamed macrophages and inhibit TNFα secretion by the macrophages. Those nanoparticles have a diameter of ∼380 nm and zeta potential of -8 mV at pH 7.2, and are non-cytotoxic. Complexation, interactions and protection from RNAse between TNFα siRNA and polyethyleneimine were higher than those using chitosan. Importantly, complexation between TNFα siRNA and polyethyleneimine facilitated higher rates of siRNA loading into nanoparticles, compared to Chi or free siRNA mixed with Lipofectamine. Oral administration of encapsulated TNFα siRNA-loaded nanoparticles specifically reduced the TNFα expression/secretion in colonic tissue in LPS-treated mice. In conclusion, we have shown: (1) that proposed TNFα siRNA-loaded NPs are prepared via a non-denaturing synthetic process; (2) a high encapsulation rate of TNFα siRNA complexed to polyethyleneimine into NPs; (3) effective enzymatic protection of TNFα siRNA by polyethyleneimine; (4) non-cytotoxicity and biodegradability of nanoparticles loaded with polyethyleneimine/TNFα siRNA; and (5) in vitro and in vivo significant anti-inflammatory effects at low TNFα siRNA dose that is specific and restricted to the colonic cells. Our results collectively indicate that polyethyleneimine/TNFα siRNA nanocomplexes represent an efficient therapeutic option for diseases such as IBD.