Synthetic biology enabling access to designer polyketides.

The full potential of polyketide discovery has yet to be reached owing to a lack of suitable technologies and knowledge required to advance engineering of polyketide biosynthesis. Recent investigations on the discovery, enhancement, and non-natural use of these biosynthetic gene clusters via computational biology, metabolic engineering, structural biology, and enzymology-guided approaches have facilitated improved access to designer polyketides. Here, we discuss recent successes in gene cluster discovery, host strain engineering, precursor-directed biosynthesis, combinatorial biosynthesis, polyketide tailoring, and high-throughput synthetic biology, as well as challenges and outlooks for rapidly generating useful target polyketides.

A historical context for understanding "an eye roll test for hypnotizability" by herbert spiegel, m.d.

Herb Spiegel was known for many professional and scientific achievements. He may be best remembered for his discovery of the Eye Roll Sign (ERS) and its relation to innate trance capacity and the parallel creation and development of the Hypnotic Induction Profile (HIP). The present paper provides a historical context for understanding Herb's 1972 publication of "An Eye Roll Test for Hypnotizability" which originally appeared in the American Journal of Clinical Hypnosis 38 years ago and is reprinted in this journal issue.

Herbert Spiegel, MD, a man for all seasons: early personal and professional development, 1914-1946.

Herbert Spiegel, MD, was a pioneer in American psychiatry and the field of hypnosis, which he first started using as an army psychiatrist posted at Fort Meade, Maryland. He served as a battalion surgeon during the invasion of North Africa and later in the Tunisian campaign. On the battlefield, Spiegel used hypnosis for quick symptom resolution and pain control. He was wounded in action on May 7, 1943, and was awarded a Purple Heart for his courage and bravery. When Spiegel was evacuated back to America, he began writing about short-term treatment strategies based on cognitive restructuring, hypnosis, and other clinical techniques. This article details his early life and career.

A historical context for understanding "An eye roll test for hypnotizability" by Herbert Spiegel, M.D.

Herb Spiegel was known for many professional and scientific achievements. He is may be best remembered for his discovery of the Eye Roll Sign (ERS) and its relation to innate trance capacity and the parallel creation and development of the Hypnotic Induction Profile (HIP). The present paper provides a historical context for understanding Herb's 1972 publication of "An Eye Roll Test for Hypnotizability" which originally appeared in the American Journal of Clinical Hypnosis 38 years ago and is reprinted in this journal issue.