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Investigating natural processes in arsenic (As) polluted areas and plants that have naturally chosen to grow there pose practical restoration recommendations. This study aimed to assess long-term changes in natural As dendroremediation dynamics for Quercus robur L. and Tilia cordata Mill., tree species capable of growing in areas polluted by mining activities. We examined total As and its forms, as well as B, Ca, K, Mg, Na and P, in soil and trees over 5 years. We also characterized pH and EC of soil, examined proline content in tree organs, and calculated Bioconcentration Factor (BCF) and Translocation Factor (TF) for As. Initial As concentrations in soil were 37.0 mg kgâ»1 under Q. robur and 34.7 mg kgâ»1 under T. cordata, significantly decreasing after 5 years to 10.5 mg kgâ»1 and 9.51 mg kgâ»1, respectively. This corresponds to pollution reduction of up to 71.8%. A notable decrease in As(III) and dimethylarsinic acid, along with increase in other organic As forms in soil, was observed. Additionally, concentrations of essential elements in soil, as well as its pH and EC, decreased over time. Both tree species accumulated substantial amounts of As in their organs, but the dynamics of this process were species-specific. During first 4 years, T. cordata accumulated more As and exhibited higher BCF, but in the 5th year, it was clearly surpassed by Q. robur. The highest TF was calculated for Q. robur in year 3, and for T. cordata in years 2 and 3. Generally, limited aboveground movement of As was indicated: BCF >1 were calculated for years 2 and 3, while TF were consistently <1. Proline content increased significantly in all organs, correlating with As, especially in Q. robur. In contrast, Q. robur leaves mapping revealed stable macroelement distributions, but clear variations were observed for T. cordata., which may suggest specific reaction to stress. These findings suggest that both species can effectively restore As-polluted areas, though with different dynamics. The selection of species for dendrorestoration should be based on whether the goal is faster remediation with lesser overall reduction (e.g. T. cordata) or slower remediation with ultimately greater pollution reduction (e.g. Q. robur).
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Glacier mice are peculiar rolling or stationary moss balls found on the surface of some glaciers. They may harbour an ecological habitat for cold-adapted invertebrates and microorganisms, but little is known about their potential to accumulate and disseminate harmful elements and substances. In this study, we investigate the presence of fallout radionuclides (137Cs, 238Pu, 239Pu, 240Pu, 210Pb) and heavy metals (Pb, As, Hg, Cd) in glacier mice and compare the results to bryophytes from adjacent glacier ecosystems. Samples were collected at Austerdalsbreen, a Norwegian outlet glacier from Jostedalsbreen ice cap. Maximum activity concentrations for bryophytes are 552 ± 12 Bq kg-1 for 137Cs, 3485 ± 138 Bq kg-1 for 210Pb, 0.0223 ± 0.065 Bq kg-1 for 238Pu and 4.34 ± 0.43 Bq kg-1 for 239+240Pu while maximum heavy metals concentrations are 70.5 mg kg-1 for Pb, 1.0 mg kg-1 for As, 1.6 mg kg-1 for Hg and 0.13 mg kg-1 for Cd. Maximum activity concentrations in cryconite are 1973.4 ± 5.0 Bq kg-1 for 137Cs, 3632 ± 593 Bq kg-1 for 210Pb, 0.51 ± 0.11 Bq kg-1 for 238Pu and 13.1 ± 1.4 Bq kg-1 for 239+240Pu and maximum heavy metal concentrations are 50.4 mg kg-1 for Pb, 3.4 mg kg-1 for As, 1.5 mg kg-1 for Hg and 0.082 mg kg-1 for Cd. We find that glacier mice show lower activity concentrations of radionuclides compared to cryoconite. The major source of plutonium isotopes is related to global fallout, whereas detected radio-cesium may be additionally affected by post-Chernobyl fallout to an unknown extent. Comparison between glacier surface and adjacent glacial habitats shows higher concentrations of heavy metals in glacier mice on the glacier ice surface and medial moraines compared to bryophytes in the glacier forefield. Glacier mice exported from a receding glacier may affect the cycling of radioactive and metal pollutants in developing proglacial ecosystems.
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Cubierta de Hielo , Metales Pesados , Metales Pesados/análisis , Noruega , Cubierta de Hielo/química , Animales , Monitoreo del Ambiente , Ceniza Radiactiva/análisis , Briófitas/química , Radioisótopos , Radioisótopos de Cesio/análisisRESUMEN
The efficiency of element accumulation depends on numerous factors, where the physico-chemical characteristics of the soil seem to be very important, and the role of taxonomic rank in the accumulation of elements by mushrooms seems to be important. The aim of the study was to compare the mineral composition of 7 species belonging to Leccinum and Suillus genera, collected between 2019 and 2021 from localizations in the west-central part of Poland. The research aimed to indicate the role of selected soil parameters in stimulating/inhibiting the accumulation of elements by selected Boletales mushroom species and to answer the question about the role of species belonging to the genus as an indicator determining the specific mineral composition of fruiting bodies. Soil pH and other soil properties (granulometric composition, organic carbon, degree of organic matter decomposition) may significantly affect mushrooms' mineral composition. Mushroom species belonging to Leccinum genus exhibited the higher amount of essential major and trace elements than species of Suillus genus). It suggests that the affiliation of the studied mushroom species to a specific genus may affect their mineral composition, and the physicochemical properties of the soil may be responsible for the lack of a clear division in the efficiency of element(s) accumulation. Selected species contain high amounts of K, Cu, Fe, and Zn, while others, such as selected Suillus gravellei fruiting bodies, also contain As and Cd. The results described serve as an introduction to a broader scientific discussion and require many further studies to confirm the role of taxonomic ranks and the influence of soil characteristics on the accumulation of elements by fruiting bodies.
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Agaricales , Minerales , Suelo , Suelo/química , Agaricales/química , Minerales/análisis , PoloniaRESUMEN
The vast amounts of mining and metallurgical wastes containing unimaginable quantities of toxic metal(loid)s require searching for managed ways. The study aimed to long-term assess the growth, elements accumulation (As, Cd, Hg, In, Mn, Mo, Pb, Sb, Sn, Ti, Tl, Zn) and proline content in 2-year-old Tilia cordata Mill. and Quercus robur L. seedlings growing under 1 and 3% extremely polluted mining sludge (MS) after 1, 2 and 3 years. Both species were able to grow efficiently without significant differences resulting from the impact of MS. The overall rise was higher for T. cordata than for Q. robur. The accumulation ability for As, Hg, In, Mn, Mo, Pb, Ti, and Zn in the whole plant was significantly higher for T. cordata, while Cd, Sb, Sn and Tl did not differ considerably between species. The highest content was found for As, Mn and Zn (68.7, 158, and 157 mg per plant, respectively) for T. cordata after 3 years of growth. The calculated Bioconcentration Factors were the highest for Cu (1.23), In (6.86), and Zn (38.4) for Q. robur, as well as for As (1.55), Hg (3.24), Mn (32.8), Mo (1.64) and Ti (18.0) for T. cordata after 3 years. The highest Translocation Factors were observed for In (1.35) and Sn (1.25) after 3 years, as well as for Mn (2.72, 3.38, and 3.03 after 1, 2, and 3 years) for Q. robur seedlings. The proline content was higher for Q. robur, regardless of which organ was examined, and the differences increased with the time of the experiment and the amount of MS addition (possibly more sensitive to stress). Young T. cordata seedlings show much greater potential than Q. robur. This is the first time that a demonstration of the high potential of long-living trees in multi-element MS remediation has been described.
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Biodegradación Ambiental , Minería , Quercus , Contaminantes del Suelo , Tilia , Contaminantes del Suelo/análisis , Contaminantes del Suelo/metabolismo , Aguas del Alcantarillado , Metales Pesados/análisis , Suelo/química , Restauración y Remediación Ambiental/métodosRESUMEN
The mineral composition of wild-growing mushroom species is influenced by various environmental factors, particularly the chemical properties of the soil/substrate. We hypothesised that element uptake might also correlate with taxonomic classification, potentially allowing us to predict contamination levels based on mushrooms within the same taxonomic rank. This study compared the mineral composition (Ag, As, Ba, Ca, Cd, Co, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, Pb, Se, and Zn) of 16 saprotrophic mushroom species from 11 genera across 4 families and 2 orders. Among these were 13 edible and 3 inedible mushrooms, all collected from natural, wild stands in a forest in central-western Poland between 2017 and 2020. Phallus impudicus exhibited the highest mean content of Ba (together with Phallus hadriani) (6.63 and 8.61 mg kg-1, respectively), Ca (with Paralepista gilva and Stropharia rugosoannulata) (803, 735 and 768 mg kg-1, respectively), Cd (with Lycoperdon perlatum) (3.59 and 3.12 mg kg-1, respectively), Co (0.635 mg kg-1), and Fe (with P. hadriani and S. rugosoannulata) (476, 427 and 477 mg kg-1, respectively), while Macrolepiota mastoidea showed the highest content of Ag (1.96 mg kg-1), As (with Coprinus comatus) (1.56 and 1.62 mg kg-1, respectively) and Cu (with Macrolepiota procera and Chlorophyllum rhacodes) (192, 175 and 180 mg kg-1, respectively). Comparing the content of the analysed elements in the genera represented by at least two species, a similarity was observed, the same as the mean concentration in soil under these species. Soil characteristics could be a superior factor that overshadows the impact of the mushroom genus on the elements accumulation, obscuring its role as a determinant in this process. The results are not definitive evidence that belonging to a particular taxonomic rank is a prerequisite condition affecting the accumulation of all elements. A closer focus on this issue is needed.
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Agaricales , Agaricales/química , Agaricales/clasificación , Polonia , Monitoreo del Ambiente/métodos , Contaminantes del Suelo/análisis , Oligoelementos/análisis , Minerales/análisisRESUMEN
Arsenic (As) remediation is challenging due to the complex nature and the persistence of these metalloid compounds. While it may seem that differences between As forms influence have been extensively described, new findings challenge the previously accepted knowledge, particularly for woody plants. Therefore, this study focused on 2-year-old Tilia cordata Mill. seedlings early (0, 2, 4, 12, 24 h) and late (3, 7, 12, 18, 25, 33 days) responses during growth under: As(III), As(V) or dimethylarsinic acid (DMA) (0.3 mM). Time-dependent transformations of As forms, distribution in plants, and microbiological characteristics (actinobacteria, bacteria, fungi, enzyme activity) were investigated. The highest increase in total As content was observed in plants exposed to As(V) and As(III). Dynamic metalloid form changes in the solution and tree organs were indicated. The most phytotoxic was DMA. This form was the main factor limiting the growth and effective accumulation of As. Despite experimenting in hydroponics, microorganisms played an important role in As form transformations, suggesting the potential for microbial-assisted dendroremediation strategies. The study confirmed that trees can convert more toxic forms into less toxic ones (e.g. As(III) to phytochelatins - As(III)-(PC3)), whose presence in roots seedlings exposed to As(III) and As(V) has been identified. The formation of hydrophobic forms (e.g. dimethylarsinoyl lipid) in the roots of seedlings grown under As(V) was confirmed. It is the first discovery for trees, previously observed only in bacteria and algae. The dynamics of metalloid form changes indicated that T. cordata transforms As forms according to their needs, which may give tree species an advantage in phytoremediation techniques. It holds great promise for the potential of dendroremediation.
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Arsénico , Biodegradación Ambiental , Plantones , Contaminantes del Suelo , Plantones/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Arsénico/metabolismo , Contaminantes del Suelo/metabolismo , Contaminantes del Suelo/toxicidad , Árboles , Metaloides/metabolismoRESUMEN
The more yerba mate infusions that are consumed, the larger the amount of grounds generated. What is more, both the infusion and the residues after brewing remain rich elements. Therefore, a strategy for the three-stage assessment of the element content was presented. A new brewing method was based on dynamic extraction, ensuring both the ease of preparing the infusion and recovering the grounds. In turn, microwave-assisted acid mineralization was used to decompose the leaves and twigs of yerba mate before and after brewing. In total, 30 products were analyzed by ICP OES in three fractions each, i.e., dry yerba mate, infusion, and grounds, to determine up to 25 elements. The elemental composition was considered in terms of the country of origin, type, or composition of yerba mate. The extraction percentages obtained with dynamic extraction were comparable to previously used ultrasound-assisted extraction, as well as data from the literature. The three-stage strategy is a novel approach in yerba mate studies, and it may be a model procedure for the laboratory preparation of yerba mate grounds (waste that can be re-used, e.g., a natural fertilizer).
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The Arctic region is threatened by climate change and pollution caused by human activities which potentially influence the elemental concentrations available to and from the biota. To better understand this delicate balance, it is crucial to investigate the role of several factors. Therefore, we quantified the level of 43 chemical elements in soils from Elsa and Ebba Valleys, Petunia Bay, Spitsbergen, a region that has experienced lasting environmental impacts from historical mining activities. We evaluated the a) vertical sampling influence by examining the variation in element distribution between the soil upper and deeper layers, b) animal influence by verifying the role of native animals, particularly vertebrates, in introducing thought faeces elements to the soil and c) anthropogenic influence by studying the spatial geographical differences in element distribution based on the degree of human pressure between the valleys. Our analysis also includes data on soil organic matter (SOM) and mineral composition. Both valleys exhibited similar mineralogical composition, but Elsa Valley had higher concentrations of most analyzed elements compared to Ebba Valley. Despite the contribution of vertebrate feaces, no increase in element concentrations was observed in the animal-influenced soils. The sampled soil layers had similar chemical element profiles for most elements. SOM content tended to be higher in superficial soils and showed a strong positive correlation with most quantified elements. The higher concentrations in Elsa Valley reflect its past mining and mineral exploration, making this area more significantly impacted than Ebba Valley. Surprisingly, vertebrate animals do not appear to influence the concentrations of chemical elements or organic matter in soils. Our findings provide valuable insights into the legacy of past mining activities and mechanisms driving environmental change in the Arctic.
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Ambiente , Suelo , Animales , Humanos , Svalbard , Compuestos de BencilidenoRESUMEN
The optimization and application of a new hyphenated procedure for iron ionic speciation, i.e., high performance liquid chromatography (HPLC) with short cation-exchange column (50 mm × 4 mm) coupled to high resolution inductively coupled plasma optical emission spectrometry (ICP hrOES), is presented in this paper. Fe(III) and Fe(II) species were separated on the column with the mobile phase containing pyridine-2,6-dicarboxylic acid (PDCA). The total time of the analysis was approx. 5 min, with a significantly low eluent flow rate (0.5 mL min-1) compared to the literature. Additionally, a long cation-exchange column (250 mm × 4.0 mm) was used as reference. Depending on the total iron content in the sample, two plasma views were chosen, e.g., an attenuated axial (<2 g kg-1) and an attenuated radial. The standard addition method was performed for the method's accuracy studies, and the applicability was presented on three types of samples: sediments, soils, and archaeological pottery. This study introduces a fast, efficient, and green method for leachable iron speciation in both geological and pottery samples.
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Hierro , Cromatografía Líquida de Alta Presión/métodos , Hierro/análisis , Análisis Espectral , CationesAsunto(s)
Agaricales , Oligoelementos , Polonia , Madera/química , Minerales , Oligoelementos/análisisRESUMEN
In this paper, the effect of the extraction method on the concentrations of selected elements in yerba mate (Ilex paraguariensis) infusions is presented. Seven pure yerba mate samples (without additives) were selected, representing various types and countries of origin. An extensive sample preparation procedure was proposed: ultrasound-assisted extraction using two types of extractants (deionized and tap water) at two different temperatures (room and 80 °C). In parallel, the above extractants and temperatures were carried out for all samples by the classical brewing method (without ultrasound). In addition, microwave-assisted acid mineralization was carried out to determine the total content. All the proposed procedures were thoroughly investigated with certified reference material (tea leaves, INCT-TL-1). For the total content of all the determined elements, acceptable recoveries (80-116%) were obtained. All digests and extracts were analyzed by simultaneous ICP OES. For the first time, it was assessed how tap water extraction affects the percentage of extracted element concentrations.
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Plant ferritin is suggested as a good source of iron for human. Usually present in trace amounts, it was induced in legumes seeds by their sprouting in FeSO4 solution. Fortified sprouts were digested in the in vitro model of the human gastrointestinal tract. ~49% of lupine and ~ 45% of soy proteins were extracted into gastric fluid and next ~ 12% and only ~ 1% into intestine fluid from lupine and soybean, respectively. Gastric digestion released mainly ferrous iron (~ 85% from lupine and ~ 95% in soybean sprouts). Complexed iron constituted ~ 43% of total iron in intestine after lupine digestion and ~ 55% after soybean digestion. Intestine digestion doubled the total iron released from lupine sprouts (from ~ 21% up to 38%), while in soybean it increased from ~ 16% up to ~ 23%. Ferritin presence was confirmed by the specific antibodies in digestive fluids, but it is only partially extracted from sprouts during in vitro digestion.
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Hierro , Lupinus , Humanos , Hierro/metabolismo , Glycine max , Ferritinas , Verduras , DigestiónRESUMEN
Of the many environmental factors that modulate the phytoextraction of elements, little has been learnt about the role of metal interactions. The study aimed to show how different concentrations of Cu, Pb and Zn in the cultivation medium influenced the biomass, plant development and phytoextraction abilities of Acer platanoides L. seedlings. Additionally, the impact on the content and distribution of Ca, K, Mg and Na in plant parts was studied with an analysis of phenols. Plants treated with a mixture of two metals were characterised by lower biomass of leaves and higher major elements content jointly than those grown in the salt of one element. Leaves of A. platanoides cultivated in Pb5 + Zn1, Pb1 + Zn1 and Pb1 + Zn5 experimental systems were characterised by specific browning of their edges. The obtained results suggest higher toxicity to leaves of Pb and Zn present simultaneously in Knop solution than Cu and Pb or Cu and Zn, irrespective of the mutual ratio of the concentrations of these elements. Antagonism of Cu and Zn concerning Pb was clearly shown in whole plant biomass when one of these elements was in higher concentration (5 mmol L-1) in solution. In the lowest concentrations (1 mmol L-1), there was a synergism between Cu and Zn in plant roots. Plants exposed to Zn5, Cu1 + Pb5, Pb5 + Zn1 and Cu1 + Zn1 were characterised by higher total phenolic content than the rest plants. Both the presence and the concentration of other elements in the soil are significant factors that modulate element uptake, total phenolic content, and plant development.
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Acer , Metales Pesados , Contaminantes del Suelo , Cobre/análisis , Zinc/análisis , Plomo/análisis , Contaminantes del Suelo/análisis , Plantas , Suelo , Biodegradación Ambiental , Metales Pesados/análisis , Raíces de Plantas/químicaAsunto(s)
Agaricales , Metales de Tierras Raras , Suelo , Árboles , Metales de Tierras Raras/análisis , MineralesRESUMEN
This study aims to collect information about soil investigation by FTIR. As we know, the FTIR technique is most often used in organic and bioorganic chemistry, while in geochemistry FTIR spectroscopy is not used very often. Therefore, there is a problem with the identification and interpretation of the IR spectra of minerals contained in sediments and soils. The reason for this is a deficiency of data about characteristic wavenumbers for minerals. Therefore, this study reviews and sums up, in one place, published articles that are connected to an investigation of minerals from 2002 to 2021 (based on the Scopus database). Additionally, the present review highlights various analytical techniques (ATR-FTIR, DRIFT, 2D-IR, and SR-FTIR) and discusses some of them for geochemical study. Additionally, the study describes helpful tools in the data pre-processing of IR spectra (normalization, baseline correction, and spectral derivatives).
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Minerales , Suelo , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Espectrofotometría InfrarrojaRESUMEN
Due to their enzymatic and bioaccumulation faculties the use of macromycetes for the decontamination of polluted matrices seems reasonable for bioremediation. For this reason, the aim of our study was to evaluate the mycoremediation ability of Agaricus bisporus cultivated on compost mixed with flotation tailings in different quantities (1, 5, 10, 15, and 20% addition). The biomass of the fruit bodies and the content of 51 major and trace elements were determined. Cultivation of A. bisporus in compost moderately polluted with flotation tailings yielded significantly lower (the first flush) and higher (the second flush) biomass of fruit bodies, compared with the control treatment. The presence of toxic trace elements did not cause any visible adverse symptoms for A. bisporus. Increasing the addition of flotation tailings to the compost induced an elevated level of most determined elements. A significant increase in rare earth elements (both flushes) and platinum group elements (first flush only) was observed. The opposite situation was recorded for major essential elements, except for Na and Mg in A. bisporus from the second flush under the most enriched compost (20%). Nevertheless, calculated bioaccumulation factor values showed a selective accumulation capacity-limited for toxic elements (except for Ag, As, and Cd) and the effective accumulation of B, Cu, K, and Se. The obtained results confirmed that A. bisporus can be used for practical application in mycoremediation in the industry although this must be preceded by larger-scale tests. This application seems to be the most favorable for media contaminated with selected elements, whose absorption by fruiting bodies is the most efficient.
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Winter tourism can generate environmental pollution and affect microbial ecology in mountain ecosystems. This could stimulate the development of antibiotic resistance in snow and its dissemination through the atmosphere and through snow melting. Despite these potential impacts, the effect of winter tourism on the snow antibiotic resistome remains to be elucidated. In this study, snow samples subjected to different levels of anthropogenic activities and surrounding forest were obtained from the Sudety Mountains in Poland to evaluate the impact of winter tourism on snow bacteria using a metagenomic approach. Bacterial community composition was determined by the sequencing of the V3-V4 hypervariable region of the 16S rRNA gene and the composition of the antibiotic resistome was explored by metagenomic sequencing. Whereas environmental factors were the main drivers of bacterial community and antibiotic resistome composition in snow, winter tourism affected resistome composition in sites with similar environmental conditions. Several antibiotic resistance genes (ARGs) showed a higher abundance in sites subjected to human activities. This is the first study to show that anthropogenic activities may influence the antibiotic resistome in alpine snow. Our results highlight the need to survey antibiotic resistance development in anthropogenically polluted sites.
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The shrinking of the Aral Sea represents one of the greatest ecological disasters of modern time. The data on the surviving northern part (Small Aral) is scarce and requires an update. This study aimed to analyze the chemistry, phyto- and zooplankton composition, and their relation in the waters of the Small Aral and its tributary, Syr Darya River. The chemistry of both ecosystems was significantly different. Small Aral was characterized by higher ionic concentrations, salinity, and electric conductivity and more significant spatial variation of chemical properties. The area near the river mouth was more pristine, while the ions concentration and salinity in the distant bays were much higher (>10). The highest concentrations of nitrates and total phosphorus in the Syr Darya were observed near Kyzylorda, indicating urban pollution. Overall, 109 phytoplankton taxa were identified in both ecosystems, with diatoms, green algae, and cyanobacteria being most abundantly represented. Oligohalobes dominated, but no polyhalobes and euhalobes algal species were identified. In total, 27 taxa of zooplankton were identified in both studied ecosystems, with the domination of rotifers over microcrustaceans. An exceptionally high level of dominance (65-91%) of rotifer Keratella cochlearis in the Syr Darya was found. The phyto- and zooplankton species richness was higher in the Syr Darya. Plankton communities of the Small Aral reflected horizontal variability of chemical properties. The total phosphorus promoted the prevalence of diatoms, rotifers, and crustaceans. Increased nitrogen concentration promoted cyanobacteria, chlorophytes, cryptophytes and chrysophytes, and rotifers Keratella cochlearis and K. quadrata. The abundance of dinophytes, diatoms Navicula cryptotenella and Cocconeis placentula, green algae Mychonastes jurisii and rotifer Keratella tecta was driven by the higher alkalinity and conductivity/salinity levels. The results represent a reference point for future monitoring of the area and add to understanding the complexity of biological transformations in the Aral Sea and its tributary.
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Cianobacterias , Diatomeas , Rotíferos , Animales , Ecosistema , Nitrógeno , Fósforo , Fitoplancton , Plancton , Ríos/química , ZooplanctonRESUMEN
Iron (Fe) treatment is one of the most commonly used methods to restore eutrophic lakes and reservoirs. The Fe-based coagulants dosage results in an almost immediate improvement in water quality at a relatively low cost. However, the effects of the application of coagulants are not always predictable, and the scale of the risks is not fully understood. The dosage of coagulants changes the chemical and physical properties of water, thereby affecting aquatic biocenoses. In this study, several laboratory experiments were conducted to evaluate the effects of Fe-based coagulant dosage on two bivalves species: Anodonta anatina and Unio tumidus. Their ability to efficiently filter water and reduce seston makes them a key component of aquatic ecosystems in terms of maintaining proper ecological health and stable functioning. Behavioral response, biochemical parameters, and body chemistry changes in mussels exposed to different doses of coagulant were surveyed. A dose-dependent reduction in filtration activity of both species was observed. As early as 10 g Fe m2 (which is a moderate dose used in lakes restoration), mussels of both species almost completely reduced their filtration activity and remained with closed valves for several subsequent days. Significant Fe accumulation in muscles of bivalves exposed to coagulant was also observed. This was particularly the case when very high doses of coagulant were applied. Then, the iron content in leg muscles of both species increased over fourfold. At the same time, a decrease in muscles calcium and phosphorus content was observed. No symptoms of oxidative stress (TBARS, H2O2) after mussels exposure to coagulants were found. The results suggest that the application of Fe-based coagulant for water ecosystem restoration may be a threat to the mussels population. These findings are significant for decisions on the selection of restoration methods for a specific lake.