A comparison of the growth and development of pyjama sharks (Poroderma africanum) in wild and captive populations.

The pyjama shark (Poroderma africanum) is a Scyliorhinid species endemic to South Africa. Even though it is kept in captivity in many aquaria, there is a lack of research on its growth and development. In this study, we investigated the fertilization rate of eggs and the age at female sexual maturation in captive sharks and compared their growth to that of wild individuals. This is the first study to compare the growth of captive and wild catsharks as well as the first study to compare growth in male and female pyjama sharks and benefits from a much larger sample size than has previously been collected. The mean incubation rate (±standard error of the mean [SE]) was 239.46 ± 4.97 days, the mean Lt of hatchlings (±SE) was 14.65 ± 0.24 cm, and the mean Wt (±SE) was 17.19 ± 0.75 g. The observed ratio of male to female offspring (1:3.5) was also significantly different from 1:1. One female laid fertilized eggs 6.6 years after hatching and was considered sexually mature. Both in captivity and in the wild, males showed negative allometric growth and females showed isometric growth. The growth performance (Φ') was also greater in captive sharks compared to wild sharks regardless of sex. However, there was significant variation in growth between individuals of the same sex. The similar growth patterns (i.e., allometry and isometry) found in wild and captive populations are a very useful tool for informing future conservation management if the population of this shark species were to decline in the future and also prove that captive studies for this species are transferable to wild populations with regard to sexual differences. This study also provides a benchmark for further captive studies in other lesser-studied catshark species and raises interesting questions concerning sexual differences in growth for other shark species.

Squalomix: shark and ray genome analysis consortium and its data sharing platform.

The taxon Elasmobranchii (sharks and rays) contains one of the long-established evolutionary lineages of vertebrates with a tantalizing collection of species occupying critical aquatic habitats. To overcome the current limitation in molecular resources, we launched the Squalomix Consortium in 2020 to promote a genome-wide array of molecular approaches, specifically targeting shark and ray species. Among the various bottlenecks in working with elasmobranchs are their elusiveness and low fecundity as well as the large and highly repetitive genomes. Their peculiar body fluid composition has also hindered the establishment of methods to perform routine cell culturing required for their karyotyping. In the Squalomix consortium, these obstacles are expected to be solved through a combination of in-house cytological techniques including karyotyping of cultured cells, chromatin preparation for Hi-C data acquisition, and high fidelity long-read sequencing. The resources and products obtained in this consortium, including genome and transcriptome sequences, a genome browser powered by JBrowse2 to visualize sequence alignments, and comprehensive matrices of gene expression profiles for selected species are accessible through https://github.com/Squalomix/info.

A sting in the tail: An embedded stingray spine in a mid-1st millennium AD adult male skeleton from Rebun Island, Hokkaido, Japan.

OBJECTIVES: We report here a stingray spine (Dasyatidae) found embedded in the femur of a male skeleton from the archaeological site of Uedomari-5, Rebun Island, Hokkaido, Japan. MATERIALS: A single well-preserved but incomplete human skeleton. METHODS: Macroscopic observation and low power magnification, CT imaging, radiocarbon dating and stable isotope (carbon, nitrogen) analysis. RESULTS: The stingray spine is tentatively identified as Bathytoshia brevicaudata. CT imaging shows no healing, indicating that death occurred shortly afterwards. The skeleton has been directly radiocarbon dated to the Okhotsk period (cal AD 429-827), with δ13C (-13.7‰) and δ15N (19.3‰) values indicating a diet focused on marine foods. CONCLUSIONS: The absence of healing in what would have been a non-lethal injury strongly suggests that the spine tipped an arrowhead, rather than being the result of an accidental encounter with a living stingray. It is possible that the injury reflects a period of increased conflict coinciding with, or following on from, the expansion of the Okhotsk culture from Sakhalin into northern Hokkaido. SIGNIFICANCE: Uedomari-5 provides the first example, to our knowledge, of a stingray spine directly embedded in human bone at an archaeological site. More widely, the finding contributes to our knowledge of conflict in northern hunter-gatherer communities. LIMITATIONS: Given the early excavation date (1949-50), there is little contextual information available for the burials. SUGGESTIONS FOR FURTHER RESEARCH: ZooMS (Zooarchaeology by Mass Spectrometry) may be able to identify the stingray species. Archival research may provide more information concerning the excavations at Uedomari-5.

Shark genomes provide insights into elasmobranch evolution and the origin of vertebrates.

Modern cartilaginous fishes are divided into elasmobranchs (sharks, rays and skates) and chimaeras, and the lack of established whole-genome sequences for the former has prevented our understanding of early vertebrate evolution and the unique phenotypes of elasmobranchs. Here we present de novo whole-genome assemblies of brownbanded bamboo shark and cloudy catshark and an improved assembly of the whale shark genome. These relatively large genomes (3.8-6.7 Gbp) contain sparse distributions of coding genes and regulatory elements and exhibit reduced molecular evolutionary rates. Our thorough genome annotation revealed Hox C genes previously hypothesized to have been lost, as well as distinct gene repertories of opsins and olfactory receptors that would be associated with adaptation to unique underwater niches. We also show the early establishment of the genetic machinery governing mammalian homoeostasis and reproduction at the jawed vertebrate ancestor. This study, supported by genomic, transcriptomic and epigenomic resources, provides a foundation for the comprehensive, molecular exploration of phenotypes unique to sharks and insights into the evolutionary origins of vertebrates.

A staging table for the embryonic development of the brownbanded bamboo shark (Chiloscyllium punctatum).

BACKGROUND: Studying cartilaginous fishes (chondrichthyans) has helped us understand vertebrate evolution and diversity. However, resources such as genome sequences, embryos, and detailed staging tables are limited for species within this clade. To overcome these limitations, we have focused on a species, the brownbanded bamboo shark (Chiloscyllium punctatum), which is a relatively common aquarium species that lays eggs continuously throughout the year. In addition, because of its relatively small genome size, this species is promising for molecular studies. RESULTS: To enhance biological studies of cartilaginous fishes, we establish a normal staging table for the embryonic development of the brownbanded bamboo shark. Bamboo shark embryos take around 118 days to reach the hatching period at 25°C, which is approximately 1.5 times as fast as the small-spotted catshark (Scyliorhinus canicula) takes. Our staging table divides the embryonic period into 38 stages. Furthermore, we found culture conditions that allow early embryos to grow in partially opened egg cases. CONCLUSIONS: In addition to the embryonic staging table, we show that bamboo shark embryos exhibit relatively fast embryonic growth and are amenable to culture, key characteristics that enhance their experimental utility. Therefore, the present study is a foundation for cartilaginous fish research. Developmental Dynamics 247:712-723, 2018. © 2017 Wiley Periodicals, Inc.