Insights from entire mitochondrial genome sequences into the phylogeny of ticks of the genera Haemaphysalis and Archaeocroton with the elevation of the subgenus Alloceraea Schulze, 1919 back to the status of a genus.

We used entire mitochondrial (mt) genome sequences (14.5-15 kbp) to resolve the phylogeny of the four main lineages of the Haematobothrion ticks: Alloceraea, Archaeocroton, Bothriocroton and Haemaphysalis. In our phylogenetic trees, Alloceraea was the sister to Archaeocroton sphenodonti, a tick of an archetypal reptile, the tuatara, from New Zealand, to the exclusion of the rest of the species of Haemaphysalis. The mt genomes of all four of the Alloceraea species that have been sequenced so far had a substantial insert, 132-312 bp, between the tRNA-Glu (E) gene and the nad1 gene in their mt genomes. This insert was not found in any of the other eight subgenera of Haemaphysalis. The mt genomes of 13 species of Haemaphysalis from NCBI GenBank were added to the most recent data set on Haemaphysalis and its close relatives to help resolve the phylogeny of Haemaphysalis, including five new subgenera of Haemaphysalis not previously considered by other authors: Allophysalis (structurally primitive), Aboimisalis (structurally primitive), Herpetobia (structurally intermediate), Ornithophysalis (structurally advanced) and Segalia (structurally advanced). We elevated Alloceraea Schulze, 1919 to the status of genus because Alloceraea Schulze, 1919 is phylogenetically distinct from the other subgenera of Haemaphysalis. Moreover, we propose that the subgenus Allophysalis is the sister to the rest of the Haemaphysalis (14 subgenera) and that the 'structurally primitive' subgenera Hoogstraal and Kim comprise early diverging lineages. Our matrices of the pairwise genetic difference (percent) of mt genomes and partial 16S rRNA sequences indicated that the mt genome sequence of Al. kitaokai (gb# OM368280) may not be Al. kitaokai Hoogstraal, 1969 but rather another species of Alloceraea. In a similar way, the mt genome sequence of H. (Herpetobia) nepalensis Hoogstraal, 1962 (gb# NC_064124) was only 2% genetically different to that of H. (Allophysalis) tibetensis Hoogstraal, 1965 (gb# OM368293): this indicates to us that they are the same species. Alloceraea cretacea may be better placed in a genus other than Alloceraea Schulze, 1919. Reptiles may have been the host to the most recent common ancestor of Archaeocroton and Alloceraea.

A mammal tick with a taste for lizard blood: Parasitism by the kangaroo soft tick, (Ornithodoros gurneyi) on sleepy lizards (Tiliqua rugosa).

The kangaroo soft tick (Ornithodoros gurneyi) inhabits xeric habitats of Australia and, as a result, little is known about the natural hosts of the different life stages of this tick. Large macropods are inferred as the kangaroo soft tick's principal hosts since all life stages inhabit the loose soil of kangaroo wallows. These ticks have been reported to parasitize bearded dragons (Pogona barbata) and sleepy lizards (Tiliqua rugosa), however it is not known whether these lizards are competent hosts of this tick. In this study the prevalence and intensity of O. gurneyi parasitism on T. rugosa was examined by sampling lizards at a long-term study site spanning a precipitation-induced ecological gradient. The competence of T. rugosa as a host for O. gurneyi was explored by keeping larvae and nymphs of O. gurneyi, that had fed on T. rugosa, in captivity to determine if they will survive and moult. We found that O. gurneyi occurs throughout the study site, but that they were seldom recorded to parasitise T. rugosa. Still, it was noted that T. rugosa is a competent host of O. gurneyi. The results of this study indicate that kangaroos are not the only competent hosts of O. gurneyi and raises the question of whether T. rugosa and other lizards may play a role in the dispersal of these ticks.

An instance of parasitism on a human by a nymph of the kangaroo soft tick, Ornithodoros gurneyi Warburton, 1926 (Acari: Argasidae) in South Australia.

The kangaroo soft tick, Ornithodoros gurneyi Warburton, 1926, is a poorly studied argasid of the more arid regions of Australia. Anecdotal accounts have stated that this tick species will bite humans, and yet bites have not been described. Herein we report on an instance of parasitism by a nymph of O. gurneyi on a human, and we provide a summary of confirmed instances of parasitism on humans by this tick based on museum specimens.

Effects of the brown anole invasion and betelnut palm planting on arthropod diversity in southern Taiwan.

The brown anole ( Anolis sagrei ) occurs naturally in various localities in Central America, and an exotic invasive population was first reported in Sheishan District, Chiayi County, Taiwan, in 2000. Previous studies showed that following the invasion of A. sagrei , the diversity and abundance of local terrestrial arthropods, such as orb spiders and arboreal insects, were severely affected. In this study, we assessed the impact of A. sagrei on arthropod diversity in Taiwan by comparing spider and insect diversities among betelnut palm plantations, in which this lizard species was either present or absent, and a secondary forest. In addition, enclosures were established in which the density of A. sagrei was manipulated to investigate the effect of this predator on spiders. The results of a lizard stomach content analysis showed that spiders comprised 7% and insects 90% of the prey consumed. Among the insects consumed by A. sagrei , more than 50% were ants. The abundances of the major arthropod prey of A. sagrei , such as jumping spiders and hymenopterans, in the lizard-present sites were much lower than in the lizard-removed sites. The enclosure experiments also showed that predation by the lizards significantly reduced the abundance of jumping spiders. All these results indicated that the introduced lizard greatly affected the diversity and abundance of terrestrial arthropods in agricultural areas in southern Taiwan.