Testing distance characteristics and reference values for ice-hockey straight sprint speed and acceleration. A systematic review and meta-analyses.

Ice-hockey requires high acceleration and speed sprint abilities, but it is unclear what the distance characteristic is for measuring these capabilities. Therefore, this systematic meta-analysis aims to summarize the sprint reference values for different sprint distances and suggest the appropriate use of ice-hockey straight sprint testing protocols. A total of 60 studies with a pooled sample of 2254 males and 398 females aged 11-37 years were included. However, the pooled data for women was not large enough to permit statistical analysis. The sprint distance used for measuring the reported acceleration and speed was between 4-48 m. Increased test distance was positively associated with increased speed (r = 0.70) and negatively with average acceleration (r = -0.87). Forward skating sprint speed increases with the measured distance up to 26 m and do not differ much from longer distance tests, while acceleration decreases with a drop below 3 m/s at distances 15 m and longer. The highest acceleration (5.89 m/s2 peak, 3.31 m/s2 average) was achieved in the shortest distances up to 7 m which significantly differs from 8-14 m tests. The highest speed (8.1 m/s peak, 6.76 m/s average) has been recorded between 26-39 m; therefore, distances over 39 m are not necessary to achieve maximum speed. Considering match demands and most reported test distances, 6.1 m is the recommended distance for peak acceleration and 30 m for peak speed. The sprint time, acceleration, and speed of each individual and the number of skating strides should be reported in future studies.

Training with a Heavy Puck Elicits a Higher Increase of Shooting Speed Than Unloaded Training in Midget Ice Hockey Players.

A method of load variability is a common way of developing specific skills in various sports, however, not explored considering the use of different ice-hockey pucks. Therefore, the purpose of this study was to compare shooting speed, shooting accuracy, and handgrip strength changes after training with variable training loads (lighter 60g pucks and heavier 260g pucks) in the wrist shot and snapshot. Sixteen male ice hockey players (13.62±0.35y; 167.67±7.71cm; 53.87±7.55kg) were subjected to a 12 week experiment during which they trained six weeks with a light puck and six weeks with a heavy puck and were tested for shooting speed, shooting accuracy and handgrip strength. The variable load training increased shooting speed (the long hand snapshot by 7.4%, the shorthand snapshot by 8.5%, and the wrist shot by 13%), shooting accuracy (by 14%), and handgrip strength (by 8.7%) of the bottom hand; all at p<0.001. Training with heavy pucks was more effective (d=0.50-0.86) than training with lighter pucks (d=23-25) for increasing puck speed. Shooting accuracy was increased by variable load training with a similar effect of heavy and light puck training. The variable training load had a positive effect on shooting speed and accuracy and the use of a heavier load was more effective than using the unloaded puck. Variable load shooting training in youth ice-hockey players is more effective with heavier pucks than lighter ones, and the improvements are greater in players with better shooting skills.

Analysis of formin functions during cytokinesis using specific inhibitor SMIFH2.

The phragmoplast separates daughter cells during cytokinesis by constructing the cell plate, which depends on interaction between cytoskeleton and membrane compartments. Proteins responsible for these interactions remain unknown, but formins can link cytoskeleton with membranes and several members of formin protein family localize to the cell plate. Progress in functional characterization of formins in cytokinesis is hindered by functional redundancies within the large formin gene family. We addressed this limitation by employing Small Molecular Inhibitor of Formin Homology 2 (SMIFH2), a small-molecule inhibitor of formins. Treatment of tobacco (Nicotiana tabacum) tissue culture cells with SMIFH2 perturbed localization of actin at the cell plate; slowed down both microtubule polymerization and phragmoplast expansion; diminished association of dynamin-related proteins with the cell plate independently of actin and microtubules; and caused cell plate swelling. Another impact of SMIFH2 was shortening of the END BINDING1b (EB1b) and EB1c comets on the growing microtubule plus ends in N. tabacum tissue culture cells and Arabidopsis thaliana cotyledon epidermis cells. The shape of the EB1 comets in the SMIFH2-treated cells resembled that of the knockdown mutant of plant Xenopus Microtubule-Associated protein of 215 kDa (XMAP215) homolog MICROTUBULE ORGANIZATION 1/GEMINI 1 (MOR1/GEM1). This outcome suggests that formins promote elongation of tubulin flares on the growing plus ends. Formins AtFH1 (A. thaliana Formin Homology 1) and AtFH8 can also interact with EB1. Besides cytokinesis, formins function in the mitotic spindle assembly and metaphase to anaphase transition. Our data suggest that during cytokinesis formins function in: (1) promoting microtubule polymerization; (2) nucleating F-actin at the cell plate; (3) retaining dynamin-related proteins at the cell plate; and (4) remodeling of the cell plate membrane.

Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production.

Nitrogen-fixing rhizobia and legumes have developed complex mutualistic mechanism that allows to convert atmospheric nitrogen into ammonia. Signalling by mitogen-activated protein kinases (MAPKs) seems to be involved in this symbiotic interaction. Previously, we reported that stress-induced MAPK (SIMK) shows predominantly nuclear localization in alfalfa root epidermal cells. Nevertheless, SIMK is activated and relocalized to the tips of growing root hairs during their development. SIMK kinase (SIMKK) is a well-known upstream activator of SIMK. Here, we characterized production parameters of transgenic alfalfa plants with genetically manipulated SIMK after infection with Sinorhizobium meliloti. SIMKK RNAi lines, causing strong downregulation of both SIMKK and SIMK, showed reduced root hair growth and lower capacity to form infection threads and nodules. In contrast, constitutive overexpression of GFP-tagged SIMK promoted root hair growth as well as infection thread and nodule clustering. Moreover, SIMKK and SIMK downregulation led to decrease, while overexpression of GFP-tagged SIMK led to increase of biomass in above-ground part of plants. These data suggest that genetic manipulations causing downregulation or overexpression of SIMK affect root hair, nodule and shoot formation patterns in alfalfa, and point to the new biotechnological potential of this MAPK.

The Specificity of Motor Learning Tasks Determines the Kind of Skating Skill Development in Older School-Age Children.

The specificity of motor learning tasks for skating development in older school-age children has not been sufficiently explored. The main objective was to compare the effects of training programs using change-of-direction (COD) speed exercises and partial skating task (SeqT) training on speed and agility performance in U12 ice hockey players. Thirteen young ice hockey males (13 ± 0.35 years, 41.92 ± 9.76 kg, 152.23 ± 9.41 cm) underwent three straight speed (4 and 30 m with and without a puck) and agility testing sessions before and after six weeks of COD training and then after a six-week intervention involving partial skating task (SeqT) training. The statistics were performed using magnitude-based decision (MBD) analysis to calculate the probability of the performance change achieved by the interventions. The MBD analysis showed that COD training had a large effect (11.7 ± 2.4% time decrease) on skating start improvement (straight sprint 4 m) and a small effect (-2.2 ± 2.4%) on improvement in agility with a puck. Partial skating task (SeqT) training had a large effect (5.4 ± 2.5%) on the improvement of the 30-m sprint with a puck and moderate effect on agility without a puck (1.9 ± 0.9%) and likely improved the 30-m sprint without a puck (2.6 ± 1.3%). COD training on the ice improves short starts and agility with a puck, while partial skating tasks (SeqT) target longer 30-m sprints and agility without a puck. Therefore, both types of training should be applied in accordance with motor learning tasks specific to current training needs.

Tissue culture, genetic transformation, interaction with beneficial microbes, and modern bio-imaging techniques in alfalfa research.

Current research needs to be more focused on agronomical plants to effectively utilize the knowledge obtained from model plant species. Efforts to improve legumes have long employed common breeding tools. Recently, biotechnological approaches facilitated the development of improved legumes with new traits, allowing them to withstand climatic changes and biotic stress. Owing to its multiple uses and profits, alfalfa (Medicago sativa L.) has become a prominent forage crop worldwide. This review provides a comprehensive research summary of tissue culture-based genetic transformation methods, which could be exploited for the development of transgenic alfalfa with agronomically desirable traits. Moreover, advanced bio-imaging approaches, including cutting-edge microscopy and phenotyping, are outlined here. Finally, characterization and the employment of beneficial microbes should help to produce biotechnologically improved and sustainable alfalfa cultivars.

Off-Ice Agility Provide Motor Transfer to On-Ice Skating Performance and Agility in Adolescent Ice Hockey Players.

Agility plays a crucial role in ice hockey training, and it can be developed directly on the ice or by additional off-ice training. Since the effectiveness of on-ice and off-ice training on players' agility have not been previously described, the purpose of this research is to compare the effects of on-ice and off-ice agility training on skating performance. Fourteen ice hockey players performed agility training on-ice for 4 weeks and off-ice for 4 weeks in a crossover design; they were tested before the agility program, after the first month and after finishing both training programs. The players were randomly assigned into one of two groups (n = 7 in each group), either performing the on-ice training protocol first (Ice1) followed by the off-ice agility training or performing the off-ice protocol first and the on-ice training second (Ice2). The test battery included straight sprints to 6.1 m and 35 m and the S corner test, test with break, weave agility with puck test and reactive agility test. The magnitude based decision showed the effect of agility training in both groups in the weave agility (Ice1, 2.9±2.8% likely improvement; Ice2, 3.1±2.5% possible improvement) and reactive agility tests (Ice1, 3.1 ±2.5% likely improvement; Ice2, 1.7±2.1% possible improvement), where the Ice1 protocol resulted in a likely positive change and Ice2 resulted in a possible positive change. The comparison of the training effect resulted in a possibly harmful change of performance in Ice2 protocol (-0.5 ± 8.9%) compared to Ice1 protocol (-1.0 ± 5.1%). On-ice training is more effective in the development of specific types of agility in adolescent U16 players. However, there is evidence that off-ice agility have motor transfer to on-ice agility. Therefore, we recommend developing on-ice agility with additional off-ice agility training during the ice hockey season.

Biochemical and Genetic Interactions of Phospholipase D Alpha 1 and Mitogen-Activated Protein Kinase 3 Affect Arabidopsis Stress Response.

Phospholipase D alpha 1 (PLDα1, AT3G15730) and mitogen-activated protein kinases (MAPKs) participate on signaling-dependent events in plants. MAPKs are able to phosphorylate a wide range of substrates putatively including PLDs. Here we have focused on functional regulations of PLDα1 by interactions with MAPKs, their co-localization and impact on salt stress and abscisic acid (ABA) tolerance in Arabidopsis. Yeast two-hybrid and bimolecular fluorescent assays showed that PLDα1 interacts with MPK3. Immunoblotting analyses likewise confirmed connection between both these enzymes. Subcellularly we co-localized PLDα1 with MPK3 in the cortical cytoplasm close to the plasma membrane and in cytoplasmic strands. Moreover, genetic interaction studies revealed that pldα1mpk3 double mutant was resistant to a higher salinity and showed a higher tolerance to ABA during germination in comparison to single mutants and wild type. Thus, this study revealed importance of new biochemical and genetic interactions between PLDα1 and MPK3 for Arabidopsis stress (salt and ABA) response.

Recent Advances in the Cellular and Developmental Biology of Phospholipases in Plants.

Phospholipases (PLs) are lipid-hydrolyzing enzymes known to have diverse signaling roles during plant abiotic and biotic stress responses. They catalyze lipid remodeling, which is required to generate rapid responses of plants to environmental cues. Moreover, they produce second messenger molecules, such as phosphatidic acid (PA) and thus trigger or modulate signaling cascades that lead to changes in gene expression. The roles of phospholipases in plant abiotic and biotic stress responses have been intensively studied. Nevertheless, emerging evidence suggests that they also make significant contributions to plants' cellular and developmental processes. In this mini review, we summarized recent advances in the study of the cellular and developmental roles of phospholipases in plants.

Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy.

Phospholipase D alpha 1 (PLDα1, At3g15730) and its product phosphatidic acid (PA) are involved in a variety of cellular and physiological processes, such as cytoskeletal remodeling, regulation of stomatal closure and opening, as well as biotic and abiotic stress signaling. Here we aimed to study developmental expression patterns and subcellular localization of PLDα1 in Arabidopsis using advanced microscopy methods such as light-sheet fluorescence microscopy (LSFM) and structured illumination microscopy (SIM). We complemented two knockout pldα1 mutants with a YFP-tagged PLDα1 expressed under the PLDα1 native promoter in order to study developmental expression pattern and subcellular localization of PLDα1 in Arabidopsis thaliana under natural conditions. Imaging of tissue-specific and developmentally-regulated localization of YFP-tagged PLDα1 by LSFM in roots of growing seedlings showed accumulation of PLDα1-YFP in the root cap and the rhizodermis. Expression of PLDα1-YFP in the rhizodermis was considerably higher in trichoblasts before and during root hair formation and growth. Thus, PLDα1-YFP accumulated in emerging root hairs and in the tips of growing root hairs. PLDα1-YFP showed cytoplasmic subcellular localization in root cap cells and in cells of the root transition zone. In aerial parts of plants PLDα1-YFP was also localized in the cytoplasm showing enhanced accumulation in the cortical cytoplasmic layer of epidermal non-dividing cells of hypocotyls, leaves, and leaf petioles. However, in dividing cells of root apical meristem and leaf petiole epidermis PLDα1-YFP was enriched in mitotic spindles and phragmoplasts, as revealed by co-visualization with microtubules. Finally, super-resolution SIM imaging revealed association of PLDα1-YFP with both microtubules and clathrin-coated vesicles (CCVs) and pits (CCPs). In conclusion, this study shows the developmentally-controlled expression and subcellular localization of PLDα1 in dividing and non-dividing Arabidopsis cells.

Developmental Nuclear Localization and Quantification of GFP-Tagged EB1c in Arabidopsis Root Using Light-Sheet Microscopy.

The development of the root apex is determined by progress of cells from the meristematic region to the successive post-mitotic developmental zones for transition, cell elongation and final cell differentiation. We addressed root development, tissue architecture and root developmental zonation by means of light-sheet microscopic imaging of Arabidopsis thaliana seedlings expressing END BINDING protein 1c (EB1c) fused to green fluorescent protein (GFP) under control of native EB1c promoter. Unlike the other two members of the EB1 family, plant-specific EB1c shows prominent nuclear localization in non-dividing cells in all developmental zones of the root apex. The nuclear localization of EB1c was previously mentioned solely in meristematic cells, but not further addressed. With the help of advanced light-sheet microscopy, we report quantitative evaluations of developmentally-regulated nuclear levels of the EB1c protein tagged with GFP relatively to the nuclear size in diverse root tissues (epidermis, cortex, and endodermis) and root developmental zones (meristem, transition, and elongation zones). Our results demonstrate a high potential of light-sheet microscopy for 4D live imaging of fluorescently-labeled nuclei in complex samples such as developing roots, showing capacity to quantify parameters at deeper cell layers (e.g., endodermis) with minimal aberrations. The data presented herein further signify the unique role of developmental cell reprogramming in the transition from cell proliferation to cell differentiation in developing root apex.