RESUMEN
Chiral enamine N-oxides have been synthesised by a diastereoselective intermolecular reverse-Cope cycloaddition reaction between chiral hydroxylamines and activated acetylenes. Their structures have been investigated by NMR, X-ray crystallography and computational methods.
Asunto(s)
Óxidos/química , Cristalografía por Rayos X , Reacción de Cicloadición , Espectroscopía de Resonancia Magnética , Modelos Moleculares , Óxidos/síntesis química , EstereoisomerismoRESUMEN
A bifunctional molecular linker containing both aryl diazonium and trioxolane groups was synthesised and its ability to sequentially functionalise glassy carbon and covalently immobilise heme investigated. Functionalisation was demonstrated by electrochemical techniques.
Asunto(s)
Carbono/química , Compuestos de Diazonio/química , Hemo/química , Compuestos Heterocíclicos con 1 Anillo/química , Técnicas Electroquímicas , Propiedades de SuperficieRESUMEN
The synthesis of uridine monomers containing either a 2'-deoxy-2'-C-methylcyano or ethylcyano group is described. These monomers are intended for incorporation into oligonucleotides to investigate a proposed duplex-stabilising effect exerted by 2'-tethered amide groups.
Asunto(s)
Nucleótidos de Pirimidina/síntesis química , Uridina/análogos & derivados , Uridina/síntesis químicaRESUMEN
A novel nucleoside-derived macrocycle formed from three thymidine 5'-carboxylic acid monomer units is described.
Asunto(s)
Nucleósidos/síntesis química , Nucleótidos Cíclicos/síntesis química , Timidina/análogos & derivados , Ácidos Carboxílicos/química , Nucleósidos/químicaRESUMEN
For the first time a fully automated procedure has been developed for the incorporation of a 3'-S-phosphorothiolate linkage into DNA, using phosphorothioamidite monomers. Coupling yields with either of the activators 5-ethylthiotetrazole or 4,5-dicyanoimidazole were in the range of 80-90%. Coupling yields were equally good when performed on either a 0.2 or 1 mumole reaction column, thus facilitating large scale synthesis.
Asunto(s)
Oligonucleótidos/síntesis química , Compuestos Organotiofosforados/síntesis química , Tionucleótidos/síntesis química , Timidina/análogos & derivadosRESUMEN
OBJECTIVE: To examine the effect of high levels of pre-intensive care unit (ICU) discharge care, as assessed by the Therapeutic Intervention Scoring System (TISS), on subsequent hospital mortality. DESIGN: A 1-year prospective, observational study. SETTING: The ICU and wards of a university teaching hospital with no high dependency facility (HDU). PATIENTS: A total of 283 patients were discharged to hospital wards between July 1997 and June 1998. ++ RESULTS: 11 % of all ICU discharges subsequently died in hospital. Patients discharged with a TISS of 20 or greater had a 21.4 % mortality compared to 3.7 % for those with a TISS of less than 10. Increasing age, Acute Physiology Score (APS) on admission and male sex were also significantly associated with post-discharge death. CONCLUSIONS: In a hospital without HDU facilities, patients who are receiving HDU levels of care on discharge from the ICU have a high in-hospital mortality.
