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1.
Parasitol Res ; 123(2): 142, 2024 Feb 23.
Artículo en Inglés | MEDLINE | ID: mdl-38393400

RESUMEN

Sarcocystis are Apicomplexan protozoa with a dixenous life cycle that includes a predator and a prey as definitive and intermediate hosts, respectively. Domestic and wild pigs are intermediate hosts of S. suihominis, with formation of sarcocysts in their muscles, while humans and non-human primates act as final hosts. After ingesting raw or undercooked sarcocyst-infested pork, signs of gastroenteritis including inappetence, nausea, vomiting, and diarrhea may develop in humans. Moreover, excretion of infective forms with human feces leads to dissemination of the parasite in the environment. In this study, macroscopic sarcocysts of white color, oval shape, and a diameter of approximately 3-8 mm were found in the skeletal muscle of a slaughtered domestic pig (Sus scrofa domesticus) destined for human consumption in an abattoir of Makurdi, Benue State, Nigeria. Sarcocyst DNA was used as template to PCR amplify the near-complete length of the 18S rRNA gene and a fragment of the cytochrome c oxidase subunit 1 (cox-1) gene. Amplicons were sequenced and used to construct phylogenetic trees with selected available Sarcocystis spp. sequences. In both cases, the placement of the analyzed sequences with S. suihominis was strongly supported, confirming the species identity of this macroscopic sarcocyst-forming parasite. This constitutes the first molecular identification of S. suihominis in Nigeria and the African continent. Proximity between pigs and humans, and poor sanitary conditions frequently encountered in pig farms of Nigeria might favor the dissemination of this zoonotic parasite, posing a threat to public health.


Asunto(s)
Sarcocystis , Sarcocistosis , Animales , Humanos , Porcinos , Sarcocystis/genética , Sarcocistosis/veterinaria , Sarcocistosis/parasitología , Filogenia , Nigeria , ARN Ribosómico 18S/genética , Músculo Esquelético , Sus scrofa
2.
Trop Anim Health Prod ; 42(8): 1605-10, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20582719

RESUMEN

The occurrence of African swine fever (ASF) DNA in slaughtered pigs in the major pig producing areas of Plateau state over a 2-year period was investigated. Three hundred fifty-nine pig tissue samples from five local government councils (LGCs) were analyzed by clinical signs (C/S), postmortem (PM) lesions and polymerase chain reaction (PCR). The results of diagnosis made by C/S and PM were compared to results obtained by PCR. Out of the 359 abattoir samples, 13 (3.62%) were positive by examination of C/S and PM lesions while 346 (96.38%) were negative. Jos-north LGC had the highest occurrence of PCR positive samples (31 samples); Panyam in Mangu LGC had no positive result. PCR analysis identified 53 positive samples (14.76%); more than 40 were identified on the field. Of the samples, 306 were PCR negative, thus giving a true ASF status of pigs in Plateau state. Analysis of the results, variables involved in the ASF spread and predictable effects of such findings in the pig industry in Plateau state and Nigeria as a whole is discussed.


Asunto(s)
Virus de la Fiebre Porcina Africana/genética , Fiebre Porcina Africana/epidemiología , Fiebre Porcina Africana/patología , Enfermedades Endémicas/veterinaria , Animales , Nigeria/epidemiología , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , Porcinos
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