RESUMEN
Crab-eating (Cerdocyon thous) and Pampas foxes (Lycalopex gymnocercus) are wild canids distributed in South America. Domestic dogs (Canis lupus familiaris) and wild canids may share viral pathogens, including rabies virus (RABV), canine distemper virus (CDV), and canine parvovirus 2 (CPV-2). To characterize the virome of these wild canid species, the present work evaluated the spleen and mesenteric lymph node virome of 17 crab-eating and five Pampas foxes using high-throughput sequencing (HTS). Organ samples were pooled and sequenced using an Illumina MiSeq platform. Additional PCR analyses were performed to identify the frequencies and host origin for each virus detected by HTS. Sequences more closely related to the Paramyxoviridae, Parvoviridae and Anelloviridae families were detected, as well as circular Rep-encoding single-stranded (CRESS) DNA viruses. CDV was found only in crab-eating foxes, whereas CPV-2 was found in both canid species; both viruses were closely related to sequences reported in domestic dogs from southern Brazil. Moreover, the present work reported the detection of canine bocavirus (CBoV) strains that were genetically divergent from CBoV-1 and 2 lineages. Finally, we also characterized CRESS DNA viruses and anelloviruses with marked diversity. The results of this study contribute to the body of knowledge regarding wild canid viruses that can potentially be shared with domestic canids or other species.
Asunto(s)
Perros/virología , Zorros/virología , Viroma , Virus/clasificación , Virus/genética , Anelloviridae/clasificación , Anelloviridae/genética , Animales , Bocavirus/clasificación , Bocavirus/genética , Brasil , Virus ADN/clasificación , Virus ADN/genética , ADN Viral , Virus del Moquillo Canino/clasificación , Virus del Moquillo Canino/genética , Secuenciación de Nucleótidos de Alto Rendimiento , Ganglios Linfáticos/virología , Metagenómica , Paramyxoviridae/clasificación , Paramyxoviridae/genética , Parvoviridae/clasificación , Parvoviridae/genética , Parvovirus Canino/clasificación , Parvovirus Canino/genética , Filogenia , ARN Viral , Bazo/virología , Uruguay , Virosis/veterinaria , Virosis/virología , Virus/aislamiento & purificaciónRESUMEN
Domestic dogs share habitats with human, a fact that makes them a potential source of zoonotic viruses. Moreover, knowledge regarding possible bloodborne pathogens is important due to the increasing application of blood transfusion in dogs. In the present study, we evaluated the serum virome of 520 dogs using throughput sequencing (HTS). The serum samples were pooled and sequenced using an Illumina MiSeq platform. Our unbiased method identified prevalent canine pathogens as canine protoparvovirus 1 (canine parvovirus 2), undersearched agents as canine bocaparvovirus 1 (minute virus of canines) and canine circovirus, circular viruses closely related to viruses recently found in human samples, and new parvovirus and anelloviruses. The dog virome described in the present work furthers the knowledge concerning the viral population in domestic animals. The present data includes information regarding viral agents that are potentially transmitted through blood transfusion among dogs.
