Flight performance of pollen starved honey bees and incomplete compensation through ingestion after early life pollen deprivation.

We investigated the effect of adult honey bee pollen nutrition on the flight performance of honey bees. Therefore, caged bees were allowed to perform 30 min of defecation/training flights every second day before flight performance of pollen-fed bees and pollen-deprived bees older than 16 days were compared in a flight mill. We first fed 10 µL of 1 M glucose solution to bees, and after they metabolized this during flight, they were fed 10 µL of 2 M glucose solution for a second flight test. Pollen-deprived bees flew longer and further than pollen-fed bees in both flights. Pollen-fed bees flew faster in the early period at the beginning of flights, whereas pollen-deprived bees were faster in the final phases. Pollen-fed bees were able to raise their maximum flight speed in 2 M glucose solution flights, whereas pollen-constraint bees were not. The two groups did not differ in abdomen fresh weight, but the fresh weight of the head and thorax and dry weight of the head, thorax and abdomen were higher in pollen-fed bees. In a second experiment, we constrained pollen consumption of caged bees during the first 7 days and compared daily consumption of bees from day 8-16 to consumption of bees unrestricted in pollen. We found that pollen-deprived bees perceive the pollen shortage and try to compensate for their needs by consuming significantly more pollen at the later phase of their life than pollen-fed bees of the same age. Still, bees constrained from pollen in the first 7 days did only reach 51.1% of the lifetime consumption of unconstrained bees. This shows that bees can sense the need for essential nutrients from pollen, but their physiological apparatus does not allow them to fully compensate for their early life constraint. Pollen deprivation only in the first 7 days of worker life likewise significantly reduced fresh and dry weights of the body sections (head, thorax, and abdomen) and survival. This underlines the importance of protein consumption in a short critical period early in adult bees' lives for their development and their performance later in life.

Bee Pollen: Clinical Trials and Patent Applications.

Bee pollen is a natural cocktail of floral nectar, flower pollen, enzymes, and salivary secretions produced by honeybees. Bee pollen is one of the bee products most enriched in proteins, polysaccharides, polyphenols, lipids, minerals, and vitamins. It has a significant health and medicinal impact and provides protection against many diseases, including diabetes, cancer, infectious, and cardiovascular. Bee pollen is commonly promoted as a cost-effective functional food. In particular, bee pollen has been applied in clinical trials for allergies and prostate illnesses, with a few investigations on cancer and skin problems. However, it is involved in several patents and health recipes to combat chronic health problems. This review aimed to highlight the clinical trials and patents involving bee pollen for different cases and to present the role of bee pollen as a supplementary food and a potential product in cosmetic applications.

Does the Pollen Diet Influence the Production and Expression of Antimicrobial Peptides in Individual Honey Bees?

We investigated the importance of protein nutrition for honey bee immunity. Different protein diets (monofloral pollen of Helianthus spp., Sinapis spp., Asparagus spp., Castanea spp., a mixture of the four different pollen and the pollen substitute FeedbeeTM) were fed to honey bees in cages ad libitum. After 18 days of feeding, apidaecin 1 isoforms concentration in the thorax were measured using nanoflow liquid chromatography coupled with mass spectrometry. Expression levels of genes, coding for apidaecins and abaecin in the abdomen were determined using quantitative PCR. The results indicate that protein-containing nutrition in adult worker honey bees can trigger certain metabolic responses. Bees without dietary protein showed lower apidaecin 1 isoforms concentrations. The significantly lowest concentration of apidaecin 1 isoforms was found in the group that was fed no pollen diet when compared to Asparagus, Castanea, Helianthus, and Sinapis pollen or the pollen supplement FeedBeeTM. Expression levels of the respective genes were also affected by the protein diets and different expression levels of these two antimicrobial peptides were found. Positive correlation between concentration and gene expression of apidaecins was found. The significance of feeding bees with different protein diets, as well as the importance of pollen nutrition for honey bee immunity is demonstrated.