RESUMEN
The chemical composition of the aerial parts of the Cape Verdean endemic shrub Artemisia gorgonum Webb (Asteraceae) was careful investigated, which led to the isolation and identification of six known furfuran lignans: eudesmin (1), magnolin (2), epimagnolin A (3), aschantin (4), kobusin (5), sesamin (6) and a flavone: artemetin (7). Compounds 1-7 were evaluated in vitro for their cytotoxicity in a screening panel consisting of various mammalian tumor cell lines, for their antimalarial activity against chloroquine-resistant Plasmodium falciparum (FcB1 strain) and for their cytotoxicity against murine normal cells (CFU-GM). While no promising cytotoxicity against human tumor cells were noticed, marginal potency and selectivity was found for compounds 1-5 against murine colon 38. Besides, compounds 2-7 showed mild antiplasmodial activities, 6 and 7 being the most active compounds (IC(50) 3.37 and 3.50 µg/ml respectively) without noticeable toxicity on mammalian normal cells. This is the first report of antiplasmodial activity for furfuran lignans and the first isolation of 1-7 from Artemisia gorgonum.
Asunto(s)
Antimaláricos/aislamiento & purificación , Artemisia/química , Flavonas/aislamiento & purificación , Lignanos/aislamiento & purificación , Plasmodium falciparum/efectos de los fármacos , Animales , Antimaláricos/farmacología , Línea Celular Tumoral , Cromatografía Líquida de Alta Presión , Flavonas/farmacología , Humanos , Técnicas In Vitro , Lignanos/farmacología , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
The chemical composition of Tornabenea bischoffii J. A. Schmidt essential oil from Cape Verde was studied by GC and GC/MS. Sixty volatile compounds were identified of which myristicin was the major constituent (33.6%). Using the 2,2-diphenyl-2-picrylhydrazyl free-radical scavenging method and the in vitro assay for prevention of lipid peroxidation by thiobarbituric reactive species, significant antioxidant activities were evidenced.
Asunto(s)
Antioxidantes/química , Apiaceae/química , Aceites Volátiles/química , Aceites de Plantas/química , Peroxidación de Lípido , Componentes Aéreos de las Plantas/química , Sustancias Reactivas al Ácido TiobarbitúricoRESUMEN
The chemical composition of Artemisia gorgonum Webb essential oil from Cape Verde was analyzed by GC and GC/MS. A total of 111 volatile compounds, accounting for 94.9% of the essential oil, were identified by GC and GC/MS. The major compounds were camphor (28.7%), chrysanthenone (10.8%), lavandulyl 2-methylbutanoate (9.5%), alpha-phellandrene (5.5%), lavandulyl propanoate (4.2%), camphene (4.0%), and p-cymene (3.4%). The volatile oil of this endemic plant, which is used in Cape Verdean folk medicine against several ailments, was tested for its antioxidant and antimalarial properties, and was found to exhibit free-radical scavenging on 1,1-diphenyl-2-picrylhydrazyl (DPPH), prevention of lipid peroxidation-in vitro by TBARS (thiobarbituric acid reactive species) assay, and antiplasmodial activity.
Asunto(s)
Antimaláricos/química , Artemisia/química , Depuradores de Radicales Libres/química , Aceites Volátiles/química , Aceites de Plantas/química , Antimaláricos/aislamiento & purificación , Antimaláricos/farmacología , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Cromatografía de Gases y Espectrometría de Masas , Peroxidación de Lípido/efectos de los fármacos , Aceites Volátiles/aislamiento & purificación , Aceites Volátiles/farmacología , Extractos Vegetales/químicaRESUMEN
The chemical composition of essential oil from the air-dried aerial parts of Satureja forbesii (Benth.) Briq. from Cape Verde was studied by GC and GC/MS. Thirty-nine volatile compounds were identified of which geranial (42.0%) and neral (31.2%) were the major constituents. Using the 2,2-diphenyl-2-picrylhydrazyl free-radical scavenging method and the in vitro assay for prevention of lipid peroxidation by thiobarbituric reactive species, significant activities were evidenced.
Asunto(s)
Depuradores de Radicales Libres/aislamiento & purificación , Aceites de Plantas/química , Satureja/química , Terpenos/aislamiento & purificación , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Compuestos de Bifenilo/química , Cabo Verde , Depuradores de Radicales Libres/química , Picratos/química , Componentes Aéreos de las Plantas/química , Terpenos/química , Sustancias Reactivas al Ácido Tiobarbitúrico/análisisRESUMEN
Leaves and flowers of Artemisia gorgonum (Asteraceae) collected in Fogo, Cape Verde islands, were phytochemically investigated and resulted in isolation and characterization of three guaianolides 1, 2, 5, and a secoguainolide 4, in addition to eight known guaianolides 6-11 and two known germacranolides 12, 13. Structures were elucidated by 1D and 2D NMR experiments. Careful examination of the (13)C NMR spectrum led to revision of the structure of a previously described guaianolide from 2 to 3. Most compounds exhibited mild antiplasmodial activities, ridentin (13) being the most interesting with an IC(50) of 3.8+/-0.7microgml(-1) against Plasmodium falciparum FcB1 and weak cytotoxicity in a vero cell line (IC(50) 71.0+/-3.9microgml(-1)).