Methods to Evaluate Bacterial Motility and Its Role in Bacterial-Host Interactions.

Bacterial motility is a widespread characteristic that can provide several advantages for the cell, allowing it to move towards more favorable conditions and enabling host-associated processes such as colonization. There are different bacterial motility types, and their expression is highly regulated by the environmental conditions. Because of this, methods for studying motility under realistic experimental conditions are required. A wide variety of approaches have been developed to study bacterial motility. Here, we present the most common techniques and recent advances and discuss their strengths as well as their limitations. We classify them as macroscopic or microscopic and highlight the advantages of three-dimensional imaging in microscopic approaches. Lastly, we discuss methods suited for studying motility in bacterial-host interactions, including the use of the zebrafish model.

Effect of Extender and Freezing Rate on Quality Parameters and In Vitro Fertilization Capacity of Alpaca Spermatozoa Recovered from Cauda Epididymis.

In alpacas, improvement of reproductive efficiency of male camelids is limited by the small testicular size, low spermatozoa production, and low quality of semen. In this study we aim to evaluate the effect of two extenders and two freezing rates on post-thaw quality of sperm recovered from alpaca epididymis with two methods (flushing and mincing), and to evaluate the in vitro fertilization (IVF) capacity of frozen sperm selected with two different selection methods (washing and swim-up). Sperm samples were processed with Tris-egg yolk or Bioxcell® extenders and frozen with slow freezing and fast freezing. The oocytes were coincubated with spermatozoa for 72 hours, and cleavage rates were recorded afterward. The results indicated that the recovery method did not influence sperm quality (∼70%). However, total sperm recovery was significantly lower for the flushing method than the mincing method. The sperm quality was influenced by the freezing extender (23.3% vs. 33.2%) and freezing rate (20.9% vs. 35.7%). When comparing different methods of sperm selection for IVF, no differences were observed on cleavage rate except for the fact that the concentration of sperm from swim-up method (20.6%) was significantly lower than the one obtained from the washing method (78.7%). The recovery technique of sperm does not affect sperm quality and the method of fast freezing was shown to be the most effective for cryopreservation of alpaca sperm.

Tendencias actuales para determinar la degradación de los materiales metálicos de componentes industriales / Current trends in degradation assessment on metallic materials of industrial components

En el trabajo se presentan las tendencias actuales en las metodologías para evaluar el daño de los materiales metálicos constructivos y la vida residual de las instalaciones de la industria energética, la del petróleo y otros procesos químicos. Se refirieren algunas de las ideas existentes sobre los fundamentos de la inspección de los componentes para detectar los defectos en el material metálico. Se describen los principales mecanismos de degradación de los materiales en esas instalaciones y se resumen las técnicas experimentales más empleadas in situ en el laboratorio para caracterizar esos mecanismos. Se presenta un grupo de métodos de análisis que se emplean para evaluar la degradación de los materiales y la vida residual de esas componentes.

The need to assess objectively a structural integrity analysis in nuclear and thermal power-, oil- and chemical- industry systems, represents a large challenge for engineers and researchers related to Materials Science, equipment manufactures or users. These systems share many of their problems with regards to aging mechanisms of components metallic materials, high replacement costs and increasing requirements on efficiency and safety. This paper makes an attempt to give an overview of the current trends on material damage and residual life assessment for installations of power-, oil- and chemical industry. Some of the currently existing ideas on components inspection, as an activity for damage detection are shown. A summary on mechanisms of material damage and experimental techniques for their characterization is also presented. Finally, some analytical methods with wide appliance in materials damage evaluation and residual life assessment of components are described.