Beyond the Spectrum: Unleashing the Potential of Infrared Radiation in Poultry Industry Advancements.

The poultry industry is dynamically advancing production by focusing on nutrition, management practices, and technology to enhance productivity by improving feed conversion ratios, disease control, lighting management, and exploring antibiotic alternatives. Infrared (IR) radiation is utilized to improve the well-being of humans, animals, and poultry through various operations. IR radiation occurs via electromagnetic waves with wavelengths ranging from 760 to 10,000 nm. The biological applications of IR radiation are gaining significant attention and its utilization is expanding rapidly across multiple sectors. Various IR applications, such as IR heating, IR spectroscopy, IR thermography, IR beak trimming, and IR in computer vision, have proven to be beneficial in enhancing the well-being of humans, animals, and birds within mechanical systems. IR radiation offers a wide array of health benefits, including improved skin health, therapeutic effects, anticancer properties, wound healing capabilities, enhanced digestive and endothelial function, and improved mitochondrial function and gene expression. In the realm of poultry production, IR radiation has demonstrated numerous positive impacts, including enhanced growth performance, gut health, blood profiles, immunological response, food safety measures, economic advantages, the mitigation of hazardous gases, and improved heating systems. Despite the exceptional benefits of IR radiation, its applications in poultry production are still limited. This comprehensive review provides compelling evidence supporting the advantages of IR radiation and advocates for its wider adoption in poultry production practices.

An efficient segmentation model for abnormal chicken droppings recognition based on improved deep dual-resolution network.

The characteristics of chicken droppings are closely linked to their health status. In prior studies, chicken droppings recognition is treated as an object detection task, leading to challenges in labeling and missed detection due to the diverse shapes, overlapping boundaries, and dense distribution of chicken droppings. Additionally, the use of intelligent monitoring equipment equipped with edge devices in farms can significantly reduce manual labor. However, the limited computational power of edge devices presents challenges in deploying real-time segmentation algorithms for field applications. Therefore, this study redefines the task as a segmentation task, with the main objective being the development of a lightweight segmentation model for the automated monitoring of abnormal chicken droppings. A total of 60 Arbor Acres broilers were housed in 5 specific pathogen-free cages for over 3 wk, and 1650 RGB images of chicken droppings were randomly divided into training and testing sets in an 8:2 ratio to develop and test the model. Firstly, by incorporating the attention mechanism, multi-loss function, and auxiliary segmentation head, the segmentation accuracy of the DDRNet was enhanced. Then, by employing the group convolution and an advanced knowledge-distillation algorithm, a lightweight segmentation model named DDRNet-s-KD was obtained, which achieved a mean Dice coefficient (mDice) of 79.43% and an inference speed of 86.10 frames per second (FPS), showing a 2.91% and 61.2% increase in mDice and FPS compared to the benchmark model. Furthermore, the DDRNet-s-KD model was quantized from 32-bit floating-point values to 8-bit integers and then converted to TensorRT format. Impressively, the weight size of the quantized model was only 13.7 MB, representing an 82.96% reduction compared to the benchmark model. This makes it well-suited for deployment on the edge device, achieving an inference speed of 137.51 FPS on Jetson Xavier NX. In conclusion, the methods proposed in this study show significant potential in monitoring abnormal chicken droppings and can provide an effective reference for the implementation of other agricultural embedded systems.

The characteristics of chicken droppings are closely related to their health. In this study, we developed a lightweight segmentation model for chicken droppings and evaluated its inference speed on the edge device with limited computational power. The results showed that the proposed model exhibits significant potential in the early warning of abnormal chicken droppings, which can help producers implement interventions before disease outbreaks, thereby avoiding great economic losses. Additionally, the model optimization and compression processes proposed in this study can provide an effective reference for the implementation of other embedded systems.

Design, synthesis, and anticancer evaluation of alkynylated pyrrole derivatives.

A series of alkynylated pyrrole derivatives were meticulously designed, drawing inspiration from the structure of 3-alkynylpyrrole-2,4-dicarboxylates, which were synthesized via a cyclization process involving methylene isocyanides and propiolaldehydes under mild conditions. These derivatives were subsequently subjected to evaluation for their anticancer properties against a panel of cell lines, including U251, A549, 769-P, HepG2, and HCT-116. According to the detailed analysis of structure-activity relationship, compound 12l emerged as the most promising molecule, with IC50 values of 2.29 ± 0.18 and 3.49 ± 0.30 µM toward U251 and A549 cells, respectively. Subsequent mechanistic investigations revealed that compound 12l exerts its effects by arresting the cell cycle in the G0/G1 phase and inducing apoptosis specifically in A549 cells. These innovative alkynylated pyrrole derivatives hold the potential to serve as a valuable template for the discovery of novel anticancer molecules.

Species sensitivities to artificial light at night: A phylogenetically controlled multilevel meta-analysis on melatonin suppression.

The rapid urbanization of our world has led to a surge in artificial lighting at night (ALAN), with profound effects on wildlife. Previous research on wildlife's melatonin, a crucial mechanistic indicator and mediator, has yielded inconclusive evidence due to a lack of comparative analysis. We compiled and analysed an evidence base including 127 experiments with 437 observations across 31 wild vertebrates using phylogenetically controlled multilevel meta-analytic models. The evidence comes mainly from the effects of white light on melatonin suppression in birds and mammals. We show a 36% average decrease in melatonin secretion in response to ALAN across a diverse range of species. This effect was observed for central and peripheral melatonin, diurnal and nocturnal species, and captive and free-living populations. We also reveal intensity-, wavelength-, and timing-dependent patterns of ALAN effects. Exposure to ALAN led to a 23% rise in inter-individual variability in melatonin suppression, with important implications for natural selection in wild vertebrates, as some individuals may display higher tolerance to ALAN. The cross-species evidence has strong implications for conservation of wild populations that are subject to natural selection of ALAN. We recommend measures to mitigate harmful impacts of ALAN, such as using 'smart' lighting systems to tune the spectra to less harmful compositions.

Enzymatic Catalysis in Size and Volume Dual-Confined Space of Integrated Nanochannel-Electrodes Chip for Enhanced Impedance Detection of Salmonella.

Nanochannel-based confinement effect is a fascinating signal transduction strategy for high-performance sensing, but only size confinement is focused on while other confinement effects are unexplored. Here, a highly integrated nanochannel-electrodes chip (INEC) is created and a size/volume-dual-confinement enzyme catalysis model for rapid and sensitive bacteria detection is developed. The INEC, by directly sandwiching a nanochannel chip (60 µm in thickness) in nanoporous gold layers, creates a micro-droplet-based confinement electrochemical cell (CEC). The size confinement of nanochannel promotes the urease catalysis efficiency to generate more ions, while the volume confinement of CEC significantly enriches ions by restricting diffusion. As a result, the INEC-based dual-confinement effects benefit a synergetic enhancement of the catalytic signal. A 11-times ion-strength-based impedance response is obtained within just 1 min when compared to the relevant open system. Combining this novel nanoconfinement effects with nanofiltration of INEC, a separation/signal amplification-integrated sensing strategy is further developed for Salmonella typhimurium detection. The biosensor realizes facile, rapid (<20 min), and specific signal readout with a detection limit of 9 CFU mL-1 in culturing solution, superior to most reports. This work may create a new paradigm for studying nanoconfined processes and contribute a new signal transduction technique for trace analysis application.

Eggshell biometrics for individual egg identification based on convolutional neural networks.

Individual egg identification technology has potential applications in breeding, product tracking/tracing, and anti-counterfeit. This study developed a novel method for individual egg identification based on eggshell images. A convolutional neural network-based model, named Eggshell Biometric Identification (EBI) model, was proposed and evaluated. The main workflow included eggshell biometric feature extraction, egg information registration, and egg identification. The image dataset of individual eggshell was collected from the blunt-end region of 770 chicken eggs using an image acquisition platform. The ResNeXt network was then trained as a texture feature extraction module to obtain sufficient eggshell texture features. The EBI model was applied to a test set of 1,540 images. The testing results showed that when an appropriate Euclidean distance threshold for classification was set (17.18), the correct recognition rate and the equal error rate reached 99.96% and 0.02%. This new method provides an efficient and accurate solution for individual chicken egg identification, and can be extended to eggs of other poultry species for product tracking/tracing and anti-counterfeit.

A reliable and low-cost deep learning model integrating convolutional neural network and transformer structure for fine-grained classification of chicken Eimeria species.

Chicken coccidiosis is a disease caused by Eimeria spp. and costs the broiler industry more than 14 billion dollars per year globally. Different chicken Eimeria species vary significantly in pathogenicity and virulence, so the classification of different chicken Eimeria species is of great significance for the epidemiological survey and related prevention and control. The microscopic morphological examination for their classification was widely used in clinical applications, but it is a time-consuming task and needs expertise. To increase the classification efficiency and accuracy, a novel model integrating transformer and convolutional neural network (CNN), named Residual-Transformer-Fine-Grained (ResTFG), was proposed and evaluated for fine-grained classification of microscopic images of seven chicken Eimeria species. The results showed that ResTFG achieved the best performance with high accuracy and low cost compared with traditional models. Specifically, the parameters, inference speed and overall accuracy of ResTFG are 1.95M, 256 FPS and 96.9%, respectively, which are 10.9 times lighter, 1.5 times faster and 2.7% higher in accuracy than the benchmark model. In addition, ResTFG showed better performance on the classification of the more virulent species. The results of ablation experiments showed that CNN or Transformer alone had model accuracies of only 89.8% and 87.0%, which proved that the improved performance of ResTFG was benefit from the complementary effect of CNN's local feature extraction and transformer's global receptive field. This study invented a reliable, low-cost, and promising deep learning model for the automatic fine-grain classification of chicken Eimeria species, which could potentially be embedded in microscopic devices to improve the work efficiency of researchers and extended to other parasite ova, and applied to other agricultural tasks as a backbone.

In vivo prediction of abdominal fat and breast muscle in broiler chicken using live body measurements based on machine learning.

The purpose of this study was to predict the carcass characteristics of broilers using support vector regression (SVR) and artificial neural network (ANN) model methods. Data were obtained from 176 yellow feather broilers aged 100-day-old (90 males and 86 females). The input variables were live body measurements, including external measurements and B-ultrasound measurements. The predictors of the model were the weight of abdominal fat and breast muscle in male and female broilers, respectively. After descriptive statistics and correlation analysis, the datasets were randomly divided into train set and test set according to the ratio of 7:3 to establish the model. The results of this study demonstrated that it is feasible to use machine learning methods to predict carcass characteristics of broilers based on live body measurements. Compared with the ANN method, the SVR method achieved better prediction results, for predicting breast muscle (male: R2 = 0.950; female: R2 = 0.955) and abdominal fat (male: R2 = 0.802; female: R2 = 0.944) in the test set. Consequently, the SVR method can be considered to predict breast muscle and abdominal fat of broiler chickens, except for abdominal fat in male broilers. However, further revaluation of the SVR method is suggested.

Advanced methods and implementations for the meta-analyses of animal models: Current practices and future recommendations.

Meta-analytic techniques have been widely used to synthesize data from animal models of human diseases and conditions, but these analyses often face two statistical challenges due to complex nature of animal data (e.g., multiple effect sizes and multiple species): statistical dependency and confounding heterogeneity. These challenges can lead to unreliable and less informative evidence, which hinders the translation of findings from animal to human studies. We present a literature survey of meta-analysis using animal models (animal meta-analysis), showing that these issues are not adequately addressed in current practice. To address these challenges, we propose a meta-analytic framework based on multilevel (linear mixed-effects) models. Through conceptualization, formulations, and worked examples, we illustrate how this framework can appropriately address these issues while allowing for testing new questions. Additionally, we introduce other advanced techniques such as multivariate models, robust variance estimation, and meta-analysis of emergent effect sizes, which can deliver robust inferences and novel biological insights. We also provide a tutorial with annotated R code to demonstrate the implementation of these techniques.

Effects of light color and intensity on discrimination of red objects in broilers.

Poultry are sensitive to red objects, such as comb and blood on the body surface, likely inducing injurious pecking in flocks. Light is an important factor that affects the pecking behavior of poultry. A wooden box was built to investigate the effects of Light Emitting Diode (LED) light color (warm white and cold white) and intensity (5 and 50 lux) of background light on the discrimination of red objects in broilers. A piece of red photographic paper (Paper 1) was used to simulate a red object and paired with another piece of paper (Paper 2 to 8) with a different color. Bigger number of the paired paper indicated greater color difference. The experiment consisted of three phases: adaptation, training, and test. In the adaptation phase, birds were selected for the adaptation to reduce the stress from the box. In the training phase, birds were trained to discriminate and peck at Paper 1 when paired with Paper 8 under one type of background light. Twenty-three birds were tested when the paired paper was changed from Paper 7 to 2. Each pair of paper included 12 trials for every bird, and response time to peck and proportion of choices of Paper 1 in the last 10 trials were collected. The results showed that broilers tested under 5 lux light had longer response times than broilers tested under 50 lux light (P < 0.05). When Paper 1 was paired with paper 7, broilers tested under warm white light had lower proportion of choices of Paper 1 than those tested under cold white light (P < 0.05). Color difference had a significant effect on response time of broilers (P < 0.05). Moreover, the proportion of choices of Paper 1 decreased to 50% (chance-level performance) when color of the paired paper was gradually similar to Paper 1. Conclusively, rearing broilers in warm white rather than cold white light with appropriate light intensity should be recommended to reduce damaging pecking behavior in broiler production.

Poultry are sensitive to red objects, such as comb and blood on the body surface, likely inducing injurious pecking in flocks. We built a wooden box to investigate the effects of light color (reddish and bluish) and intensity (5 and 50 lux) of background light on the discrimination of red objects in broilers. A piece of red photographic paper (Paper 1) was used and paired with another piece of paper (Paper 2 to 8) with a different color. Every bird was trained to discriminate and peck at Paper 1 when paired with Paper 8 under one type of background light. Then, Paper 8 was changed from Paper 7 to 2. Response time to peck and proportion of choices of Paper 1 were collected. We found that broilers under 5 lux light had longer response times than under 50 lux light. Broilers under reddish light had lower proportion of choices than under bluish light. Moreover, color difference had a significant effect on the response time and the proportion of choices. Conclusively, rearing broilers under reddish rather than bluish light with appropriate intensity should be recommended to reduce damaging pecking behavior in broiler production.

Effects of Monochromatic Blue Light on Reducing the Adverse Impact of Induced Cyclic Chronic Heat Stress during the Thermal Manipulation of Broiler Embryos.

Objective: The aim was to detect effects of blue light on reducing the adverse effect of heat stress in thermal manipulation (TM) of broiler embryos by subjecting embryos to heat stress during incubation development. Methods: Eggs were assigned to four treatments in which the TM (thermal manipulation) was exposed to 40°C for 4 h daily during five successive days, if TM was operated. The treatments were (1) normal temperature with white lighting group (37°C+W), (2) normal temperature with blue lighting group (37°C+B), (3) thermal manipulation with white lighting group (40°C+W), and (4) thermal manipulation with blue lighting group (40°C+B). Results: Blue light significantly lowered MDA and corticosterone concentrations in the embryonic liver. Additionally, the damage of embryonic liver tissue caused by heat stress could be reduced by blue light. HSPs and HSFs gene expression of chicken liver were modulated by blue light significantly, whereas the effects were different, respectively. Moreover, blue light modulated liver antioxidant enzyme activity and their gene expression in embryonic liver significantly. However, blue light did not exert significant effects on body weight, late hatch rectal temperature and tibia length of hatched chicks. Conclusions: The results suggest that monochromatic blue light can reduce the content of MDA and corticosterone of broiler embryos in heat stress and increase the relative expression of SOD and CAT genes. Moreover, the monochromatic blue light may reduce the metabolic heat production of broilers during the embryonic stage, thus reducing the damage of broilers due to heat stress during the embryonic heat acclimation stage.

Feather Damage Monitoring System Using RGB-Depth-Thermal Model for Chickens.

Feather damage is a continuous health and welfare challenge among laying hens. Infrared thermography is a tool that can evaluate the changes in the surface temperature, derived from an inflammatory process that would make it possible to objectively determine the depth of the damage to the dermis. Therefore, the objective of this article was to develop an approach to feather damage assessment based on visible light and infrared thermography. Fusing information obtained from these two bands can highlight their strengths, which is more evident in the assessment of feather damage. A novel pipeline was proposed to reconstruct the RGB-Depth-Thermal maps of the chicken using binocular color cameras and a thermal infrared camera. The process of stereo matching based on binocular color images allowed for a depth image to be obtained. Then, a heterogeneous image registration method was presented to achieve image alignment between thermal infrared and color images so that the thermal infrared image was also aligned with the depth image. The chicken image was segmented from the background using a deep learning-based network based on the color and depth images. Four kinds of images, namely, color, depth, thermal and mask, were utilized as inputs to reconstruct the 3D model of a chicken with RGB-Depth-Thermal maps. The depth of feather damage can be better assessed with the proposed model compared to the 2D thermal infrared image or color image during both day and night, which provided a reference for further research in poultry farming.

Wavelength-specific artificial light disrupts molecular clock in avian species: A power-calibrated statistical approach.

Nighttime lighting is an increasingly important anthropogenic environmental stress on plants and animals. Exposure to unnatural lighting environments may disrupt the circadian rhythm of organisms. However, the sample size of relevant studies, e.g. disruption of the molecular circadian clock by light pollution, was small (<10), which led to low statistical power and difficulties in replicating prior results. Here, we developed a power-calibrated statistical approach to overcome these weaknesses. The results showed that the effect size of 2.48 in clock genes expression induced by artificial light would ensure the reproducibility of the results as high as 80%. Long-wavelength light (560-660 nm) entrained expressions of the positive core clock genes (e.g. cClock) and negative core clock genes (e.g. cCry1, cPer2) in robust circadian rhythmicity, whereas those clock genes were arrhythmic in short-wavelength light (380-480 nm). Further, we found artificial light could entrain the transcriptional-translational feedback loop of the molecular clock in a wavelength-dependent manner. The expression of the positive core clock genes (cBmal1, cBmal2 and cClock), cAanat gene and melatonin were the highest in short-wavelength light and lowest in long-wavelength light. For the negative regulators of the molecular clock (cCry1, cCry2, cPer2 and cPer3), the expression of which was the highest in long-wavelength light and lowest in short-wavelength light. Our statistical approach opens new opportunities to understand and strengthen conclusions, comparing with the studies with small sample sizes. We also provide comprehensive insight into the effect of wavelength-specific artificial light on the circadian rhythm of the molecular clock in avian species. Especially, the global lighting is shifting from "yellow" sodium lamps, which is more like the long-wavelength light, toward short-wavelength light (blue light)-enriched "white" light-emitting diodes (LEDs).

The effect of 580 nm-based-LED mixed light on growth, adipose deposition, skeletal development, and body temperature of chickens.

Though previous study indicated that the 580 nm-yellow-LED-light showed an stimulating effect on growth of chickens, the low luminous efficiency of the yellow LED light cannot reflect the advantage of energy saving. In present study, the cool white LED chips and yellow LED chips have been combined to fabricate the white × yellow mixed LED light, with an enhanced luminous efficiency. A total 300 newly hatched chickens were reared under various mixed LED light. The results indicated that the white × yellow mixed LED light had "double-edged sword" effects on bird's body weight, bone development, adipose deposition, and body temperature, depending on variations in ratios of yellow component. Low yellow ratio of mixed LED light (Low group) inhibited body weight, whereas medium and high yellow ratio of mixed LED light (Medium and High groups) promoted body weight, compared with white LED light (White group). A progressive change in yellow component gave rise to consistent changes in body weight over the entire experiment. Moreover, a positive relationship was observed between yellow component and feed conversion ratio. High group-treated birds had greater relative abdominal adipose weight than Medium group-treated birds (P = 0.048), whereas Medium group-treated birds had greater relative abdominal adipose weight than Low group-treated birds (P = 0.044). We found that mixed light improved body weight by enhancing skeletal development (R2 = 0.5023, P = 0.0001) and adipose deposition (R2 = 0.6012, P = 0.0001). Birds in the Medium, High and Yellow groups attained significantly higher surface temperatures compared with the White group (P = 0.010). The results suggest that the application of the mixed light with high level of yellow component can be used successfully to improve growth and productive performance in broilers.

Effects of mixing eggs of different initial incubation time on the hatching pattern, chick embryonic development and post-hatch performance.

BACKGROUND: The hatch window that varies from 24 to 48 h is known to influence post-hatch performance of chicks. A narrow hatch window is needed for commercial poultry industry to acquire a high level of uniformity of chick quality. Hatching synchronization observed in avian species presents possibilities in altering hatch window in artificial incubation. METHODS: Layer eggs which were laid on the same day by a single breeder flock and stored for no more than two days started incubation 12 h apart to obtain developmental distinction. The eggs of different initial incubation time were mixed as rows adjacent to rows on day 12 of incubation. During the hatching period (since day 18), hatching time of individual eggs and hatch window were obtained by video recordings. Embryonic development (day 18 and 20) and post-hatch performance up to day 7 were measured. RESULTS: The manipulation of mixing eggs of different initial incubation time shortened the hatch window of late incubated eggs in the manipulated group by delaying the onset of hatching process, and improved the hatchability. Compared to the control groups, chick embryos or chicks in the egg redistribution group showed no significant difference in embryonic development and post-hatch performance up to day 7. DISCUSSION: We have demonstrated that eggs that were incubated with advanced eggs performed a narrow spread of hatch with higher hatchability, normal embryonic development as well as unaffected chick quality. This specific manipulation is applicable in industrial poultry production to shorten hatch window and improve the uniformity of chick quality.

The quantitative models for broiler chicken response to monochromatic, combined, and mixed light-emitting diode light: A meta-analysis.

Although many experiments have been conducted to clarify the response of broiler chickens to light-emitting diode (LED) light, those published results do not provide a solid scientific basis for quantifying the response of broiler chickens. This study used a meta-analysis to establish light spectral models of broiler chickens. The results indicated that 455 to 495 nm blue LED light produced the greatest positive response in body weight by 10.66% (BW; P < 0.001) and 515 to 560 nm green LED light increased BW by 6.27% (P < 0.001) when compared with white light. Regression showed that the wavelength (455 to 660 nm) was negatively related to BW change of birds, with a decrease of about 4.9% BW for each 100 nm increase in wavelength (P = 0.002). Further analysis suggested that a combination of the two beneficial light sources caused a synergistic effect. BW was further increased in birds transferred either from green LED light to blue LED light (17.23%; P < 0.001) or from blue LED light to green LED light (17.52%; P < 0.001). Moreover, birds raised with a mixture of green and blue LED light showed a greater BW promotion (10.66%; P < 0.001) than those raised with green LED light (6.27%). A subgroup analysis indicated that BW response to monochromatic LED light was significant regardless of the genetic strain, sex, control light sources, light intensity and regime of LED light, environmental temperature, and dietary ME and CP (P > 0.05). However, there was an interaction between the FCR response to monochromatic LED light with those covariant factors (P < 0.05). Additionally, green and yellow LED light played a role in affecting the meat color, quality, and nutrition of broiler chickens. The results indicate that the optimal ratio of green × blue of mixed LED light or shift to green-blue of combined LED light may produce the optimized production performance, whereas the optimal ratio of green/yellow of mixed or combined LED light may result in the optimized meat quality.

Artificial light and biological responses of broiler chickens: dose-response.

Light intensity is an important aspect for broiler production. However, previous results do not provide a solid scientific basis for quantifying the response of broilers to light intensity. This study performed a meta-analysis to model the response of broilers to 0.1-200 lux of light intensity. Meta-analysis was used to integrate smaller studies and increase the statistical power over that of any single study and explore new hypotheses. The results indicated that light intensity <5 lux caused welfare concern (P < 0.05) and light intensity <1 lux induced productivity loss of broiler (P < 0.05), whereas greater level of light intensity >10 lux led to increased mortality (P < 0.01) and decreased uniformity (P < 0.05). Meta-regression showed that 30-200 lux light intensity was negatively related to BW (P = 0.047) and feed intake change (P = 0.054), whereas a quadratic relationship was observed between feed conversion ratio change and 50-180 lux light intensity (R2 = 0.95). In addition, the majority of carcass characteristics (abdominal fat weight and wing weight) and metabolic indicators (K+, Ca2+, and T3) were affected by light intensity >5 lux. To conclude, this meta-analysis based on published data quantitatively identified that 5 lux of light intensity during grow-out period should be the minimum level to maintain a well productivity and welfare of broiler chickens.

Synthesis and evaluation of a series of pyridine and pyrimidine derivatives as type II c-Met inhibitors.

In this study, a series of novel pyridine and pyrimidine-containing derivatives were designed, synthesized and biologically evaluated for their c-Met inhibitory activities. In the biological evaluation, half of the target compounds exhibited moderate to potent c-Met inhibitory activities. Among which, it is noteworthy that compounds 13d not only showed most potent c-Met inhibitory potency but also displayed excellent anti-proliferative activity (IC50=127nM against EBC-1 cell line) as well as an acceptable kinase selectivity profile. Moreover, the western blot assay indicated that 13d inhibited c-Met phosphorylation in EBC-1 cells in a dose-dependent manner, with complete abolishment at 0.1mM. All these experimental results suggested that 13d could be served as a promising lead compound for the development of anticancer agents.

A new method to manipulate broiler chicken growth and metabolism: Response to mixed LED light system.

Present study introduced a new method to manipulate broiler chicken growth and metabolism by mixing the growth-advantage LED. We found that the green/blue LED mixed light system (G-B and G × B) have the similar stimulatory effect on chick body weight with single green light and single blue light (G and B), compared with normal artificial light (P = 0.028). Moreover, the percentage of carcass was significantly greater in the mixed light (G × B) when compared with the single light (P = 0.003). Synchronized with body weight, the mixed light (G-B and G × B) had a significant improved influence on the feed conversion of birds compared with normal light (P = 0.002). A significant improvement in feed conversion were found in mixed light (G × B) compared with single LED light (P = 0.037). G group resulted in a greater high-density lipoprotein cholesterol level than B group (P = 0.002), whereas B group resulted in a greater low-density lipoprotein cholesterol level than G group (P = 0.017). The mixed light significantly increased the birds' glucose level in comparison with the single light (P = 0.003). This study might establish an effective strategy for maximizing growth of chickens by mixed LED technology.

Physiological responses to daily light exposure.

Long daylength artificial light exposure associates with disorders, and a potential physiological mechanism has been proposed. However, previous studies have examined no more than three artificial light treatments and limited metabolic parameters, which have been insufficient to demonstrate mechanical responses. Here, comprehensive physiological response curves were established and the physiological mechanism was strengthened. Chicks were illuminated for 12, 14, 16, 18, 20, or 22 h periods each day. A quadratic relationship between abdominal adipose weight (AAW) and light period suggested that long-term or short-term light exposure could decrease the amount of AAW. Quantitative relationships between physiological parameters and daily light period were also established in this study. The relationships between triglycerides (TG), cholesterol (TC), glucose (GLU), phosphorus (P) levels and daily light period could be described by quadratic regression models. TG levels, AAW, and BW positively correlated with each other, suggesting long-term light exposure significantly increased AAW by increasing TG thus resulting in greater BW. A positive correlation between blood triiodothyronine (T3) levels and BW suggested that daily long-term light exposure increased BW by thyroid hormone secretion. Though the molecular pathway remains unknown, these results suggest a comprehensive physiological mechanism through which light exposure affects growth.