Use of grape by-products in aquaculture: New frontiers for a circular economy application.

Grape by-products have already been used in cosmetics, food industries, but also animal feed industry, especially monogastrics and in aquaculture. Grape by-products have been studied for a long time and their principal activities are antimicrobial and antioxidant. Concerning aquaculture, the great demand and necessity to replace animal sources with vegetable ones, has placed grape by-products as possible new phytonutrients with beneficial properties. The purpose of this review is to describe the use of grape by-products in aquaculture, during the last decade, concerning their effects on: 1) gut health and welfare status; 2) growth performances; 3) quality of fillets and flesh during the rearing cycle and shelf-life products. Although other studies highlighted that the high supplementation of grape by-products could negatively affect fish health and growth, due to antinutritional factors (tannins), grape by-products are proven to be valuable phytonutrients that can be incorporated into fish feed to enhance growth and health during rearing conditions. Even in fish products, their utilization has proven to elongate the properties and shelf-life of fillets and minces. Further studies to evaluate the possible integrations or replacements with grape by-products in fish feed in order to evaluate their effectiveness in aquaculture from a sustainable circular economy perspective will be desirable to enhance the use of these products.

Mercury levels in Merluccius merluccius muscle tissue in the central Mediterranean Sea: Seasonal variation and human health risk.

In this study we analysed total mercury (THg) levels in European hake (Merluccius merluccius) - an ecologically and commercially important species throughout the Mediterranean - caught in the northern and central Adriatic Sea. To the best of our knowledge, this is the first study evaluating THg levels in hake fillets in relation to ecological (season) and biological (body size, sex, sexual maturity, lipid content) parameters. THg levels in muscle showed no sex-related differences; in contrast, significant season-related differences were found in females, with higher levels in spring-summer compared with autumn-winter. No season-related differences were seen in males. A significant sex effect was found for body size and sexual maturity. Females showed a correlation between THg level and length, THg being significantly higher in mature compared with immature specimens. No significant sex effect was found for muscle lipid content, because a correlation between THg concentration and tissue lipids was found in both sexes. Since the mean THg concentration found in M. merluccius fillets (0.64 ± 0.29 mg kg-1 dry weight; range, 0.20-1.53) was consistently under the level set by EU regulations, this study demonstrates that European hake caught in the northern and central Adriatic is safe for human consumption.

Assessment of seasonal relationship between polycyclic aromatic hydrocarbon accumulation and expression patterns of oxidative stress-related genes in muscle tissues of red mullet (M. barbatus) from the Northern Adriatic Sea.

In this study, we examined the seasonal association between Polycyclic Aromatic Hydrocarbon (PAH) concentrations and mRNA expression profiles of some antioxidant genes (i.e. CAT, GST and SOD), as well as lipid peroxidation (LPO), in muscle of sexually inactive females of red mullet (Mullus barbatus). Fish were captured in a fishery area of the Northern Adriatic Sea during both winter and summer. We found significantly (p < 0.05) higher ∑HMW-PAHs concentrations in muscle of specimens caught during winter than summer. On the basis of sampling season, red mullets exhibited different gene expression profiles of antioxidant enzymes showing lower levels of both CAT and GST in winter than in summer. Accordingly, CAT was found to be negatively associated with ∑PAH concentrations, especially ∑LMW-PAH, in individuals collected during winter. Seasonal-related downregulation of some oxidative stress biomarker expression is suggestive of greater susceptibility of red mullets to PAHs during winter.