RESUMEN
The molecular basis for onset, maintenance and propagation of excitation along neuronal networks in epilepsy is still poorly understood. Besides different neurotransmitter receptors that control signal transfer at the synapse, one key regulator involved in all of these processes is the ATPase N-ethylmaleimide-sensitive fusion protein (NSF). Therefore, we analyzed receptor subunits and NSF levels in tissues from the medial temporal gyrus (MTG) of patients with pharmaco-resistant focal temporal lobe epilepsy resected during epilepsy surgery and autopsy controls. The resected tissues were further characterized by field potential recordings into tissues with and without spontaneous sharp wave activity. We detected increased levels of NSF, NMDA 1.1, 2A and GABAAγ2 receptor subunits associated with spontaneous sharp wave spiking activity. We further identified correlations between NSF, AMPA receptor subunit, metabotropic glutamate receptor and adenosine 1 receptor levels in the spontaneous sharp wave spiking tissues. Our findings suggest that NSF plays a key role in controlling spontaneous network excitation in epilepsy by two mechanisms of action: (1) directly via controlling transmitter release at the presynaptic side, and (2) indirectly via altering the function of possible receptor crosstalk and directing/integrating specific receptor compounds through/into the postsynaptic membrane.
Asunto(s)
Epilepsia del Lóbulo Temporal/metabolismo , Proteínas Sensibles a N-Etilmaleimida/metabolismo , Lóbulo Temporal/metabolismo , Adolescente , Adulto , Anciano , Niño , Preescolar , Epilepsia Refractaria/metabolismo , Epilepsia Refractaria/patología , Epilepsia Refractaria/cirugía , Epilepsia del Lóbulo Temporal/patología , Epilepsia del Lóbulo Temporal/cirugía , Femenino , Humanos , Masculino , Persona de Mediana Edad , ARN Mensajero/metabolismo , Receptores de Neurotransmisores/metabolismo , Lóbulo Temporal/patología , Técnicas de Cultivo de TejidosRESUMEN
PURPOSE: A disturbed balance between excitatory and inhibitory neurotransmission underlies epileptic activity, although reports concerning neurotransmitter systems involved remain controversial. METHODS: We quantified densities of 15 receptors in neocortical biopsies from patients with pharmacoresistant focal temporal lobe epilepsy and autopsy controls, and searched for correlations between density alterations and clinical factors or the occurrence of spontaneous synaptic potentials in vitro. KEY FINDINGS: α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate, N-methyl-d-aspartate (NMDA), peripheral benzodiazepine, muscarinic (M)(1) , M(2) , nicotinic, α(1) , α(2h) , serotonin (5-HT)(1A) , and adenosine (A)(1) receptor densities were significantly altered in biopsies. The epileptic cohort was subdivided based on clinical (febrile seizures, hippocampal sclerosis, neocortical pathologies, surgery outcome) or electrophysiologic (spontaneous field potentials) criteria, resulting in different patterns of significantly altered receptor types when comparing a given epileptic group with controls. Only AMPA, kainate, M(2) , and 5-HT(1A) receptors were always significantly altered. γ-Aminobutyric acid (GABA)(A) , GABA(B) , and 5-HT(2) receptor alterations were never significant. Correlation patterns between receptor alterations and illness duration or seizure frequency varied depending on whether the epileptic cohort was considered as a whole or subdivided. SIGNIFICANCE: Neocortical temporal lobe epilepsy is associated with a generalized receptor imbalance resulting in a net potentiation of excitatory neurotransmission. Peripheral benzodiazepine receptor alterations highlight that astrocytes are also impaired by seizure activity.
Asunto(s)
Epilepsias Parciales/metabolismo , Neocórtex/metabolismo , Receptores de Superficie Celular/metabolismo , Adulto , Anciano , Estudios de Cohortes , Electroencefalografía/métodos , Epilepsias Parciales/fisiopatología , Femenino , Humanos , Ligandos , Masculino , Persona de Mediana Edad , Unión Proteica/fisiología , Adulto JovenRESUMEN
Periventricular nodular heterotopias (PNHs) are frequently associated with pharmacoresistant epilepsy. They are considered part of a dysfunctional network, connected to the overlying cortex. Therefore, removal of the PNHs and additional cortectomy or lobectomy seem to be essential for significant and long-lasting seizure reduction. These procedures, however, can have considerable limitations, especially in patients with functional eloquent cortex adjacent to the PNH. Alternatively, stereotactic neurosurgery can reduce the surgical trauma. Presented is a 56-year-old man who became seizure-free after stereotactically guided radiofrequency lesioning of a solitary PNH.
Asunto(s)
Ablación por Catéter/métodos , Heterotopia Nodular Periventricular/cirugía , Electroencefalografía , Epilepsia/complicaciones , Humanos , Imagen por Resonancia Magnética/métodos , Masculino , Persona de Mediana Edad , Heterotopia Nodular Periventricular/etiología , Técnicas EstereotáxicasRESUMEN
BACKGROUND: Most patients do well after epilepsy surgery for mesial temporal lobe sclerosis, and in only 8 to 12% of all operations, the outcome is classified as not improved. OBJECTIVE: To analyze the outcome of reoperation in cases of incomplete resection of mesial temporal lobe structures in patients with mesial temporal lobe sclerosis in temporal lobe epilepsy. METHODS: We analyzed 22 consecutive patients who underwent reoperation for mesial temporal lobe sclerosis (follow-up, 23-112 months; mean, 43.18 months) by evaluating noninvasive electroencephalographic/video monitoring before the first and second surgeries (semiology, interictal epileptiform discharges, ictal electroencephalography with special attention to the secondary contralateral evolution of the electroencephalographic seizure pattern after the initial regionalization), and magnetic resonance imaging (resection indices after the first and second surgeries on the amygdala, hippocampus, lateral temporal lobe). In 18 of 22 patients T2 relaxometry of the contralateral hippocampus was performed. RESULTS: Nine of 22 patients became seizure free; another 4 patients had a decrease in seizures and eventually became seizure free (range, 16-51 months; mean, 30.3). Recurrence of seizures is associated with (1) ictal electroencephalography with later evolution of an independent pattern over the contralateral temporal lobe (0 of 5 patients seizure free vs 5 of 7 patients non-seizure free; P = .046) and (2) a smaller amount of lateral temporal lobe resection in the second surgery (1.06 ± 0.59 cm vs 2.18 ± 1.37 cm; P = .019). No significant correlation with outcome was found for lateralization of interictal epileptiform discharges, change in semiology, other resection indices, T2 relaxometry, onset and duration of epilepsy, duration of follow-up, and side of surgery. CONCLUSION: Patients have a less favorable outcome with a reoperation if they show ictal scalp electroencephalography with secondary contralateral propagation and if only a small second resection of the lateral temporal lobe is performed.
Asunto(s)
Epilepsia del Lóbulo Temporal/patología , Epilepsia del Lóbulo Temporal/cirugía , Hipocampo/patología , Hipocampo/cirugía , Procedimientos Neuroquirúrgicos , Adolescente , Adulto , Niño , Electroencefalografía , Epilepsia del Lóbulo Temporal/fisiopatología , Femenino , Hipocampo/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Reoperación , Esclerosis/complicaciones , Esclerosis/patología , Adulto JovenRESUMEN
We report the clinical presentation, neuroradiologic characteristics, and detailed histopathologic findings in a unique case of drug-resistant focal epilepsy due to sublobar dysplasia (SLD), treated successfully by resection of the malformed cortex. Histopathology with leptomeningeal and subcortical heterotopia, disturbance of cortical lamination and marked cortical and subcortical astrocytosis, but absence of balloon cells, points to a disorder of neuronal migration and organization rather than proliferation in SLD. The additional presence of a lateral proboscis and meningocele in our case as well as further associated callosal and cerebellar anomalies may suggest an etiologic unknown damage of pathways controlling the embryogenesis of craniofacial field processes.
Asunto(s)
Epilepsia/patología , Epilepsia/cirugía , Malformaciones del Desarrollo Cortical/patología , Malformaciones del Desarrollo Cortical/cirugía , Corteza Cerebral/anomalías , Corteza Cerebral/cirugía , Epilepsias Parciales/patología , Epilepsias Parciales/cirugía , Humanos , Procedimientos NeuroquirúrgicosRESUMEN
Angiocentric glioma has recently been described as a novel epilepsy associated tumor with distinct clinico-pathologic features. We report the clinical and pathologic findings in 8 additional cases of this rare tumor type and extend its characterization by genomic profiling. Almost all patients had a history of long-standing drug-resistant epilepsy. Cortico-subcortical tumors were located in the temporal and parietal lobes. Seizures began at 3 to 14 years of age and surgery was performed at 6 to 70 years. Histologically, the tumors were characterized by diffuse growth and prominent perivascular tumor cell arrangements with features of astrocytic/ependymal differentiation, but lacking neoplastic neuronal features. Necrosis and vascular proliferation were not observed and mitoses were sparse or absent. MIB-1 proliferation indices ranged from <1% to 5%. Immunohistochemically, all cases stained positively for glial fibrillary acidic protein, vimentin, protein S100B, variably for podoplanin, and showed epithelial membrane antigen-positive cytoplasmic dots. Electron microscopy showed ependymal characteristics in 2 of 3 cases investigated. An analysis of genomic imbalances by chromosomal comparative genomic hybridization revealed loss of chromosomal bands 6q24 to q25 as the only alteration in 1 of 8 cases. In 1 of 3 cases, a high-resolution screen by array-comparative genomic hybridization identified a copy number gain of 2 adjacent clones from chromosomal band 11p11.2 containing the protein-tyrosine phosphatase receptor type J (PTPRJ) gene. All patients are seizure free and without evidence of tumor recurrence at follow-up times ranging from 1/2 to 6.9 years. Our findings support 2 previous reports proposing that angiocentric glioma is a novel glial tumor entity of low-grade malignancy.
Asunto(s)
Neoplasias Encefálicas/genética , Neoplasias Encefálicas/ultraestructura , Epilepsia/genética , Regulación Neoplásica de la Expresión Génica , Glioma/genética , Glioma/ultraestructura , Adolescente , Adulto , Anciano , Astrocitos/patología , Neoplasias Encefálicas/química , Neoplasias Encefálicas/complicaciones , Neoplasias Encefálicas/cirugía , Diferenciación Celular , Proliferación Celular , Niño , Preescolar , Deleción Cromosómica , Cromosomas Humanos Par 11 , Cromosomas Humanos Par 6 , Epéndimo/patología , Epilepsia/patología , Epilepsia/prevención & control , Europa (Continente) , Femenino , Estudios de Seguimiento , Dosificación de Gen , Perfilación de la Expresión Génica/métodos , Proteína Ácida Fibrilar de la Glía/análisis , Glioma/química , Glioma/complicaciones , Glioma/cirugía , Humanos , Imagen por Resonancia Magnética , Masculino , Glicoproteínas de Membrana/análisis , Persona de Mediana Edad , Mucina-1/análisis , Factores de Crecimiento Nervioso/análisis , Hibridación de Ácido Nucleico , Análisis de Secuencia por Matrices de Oligonucleótidos , Proteínas Tirosina Fosfatasas Clase 3 Similares a Receptores/genética , Subunidad beta de la Proteína de Unión al Calcio S100 , Proteínas S100/análisis , Factores de Tiempo , Resultado del Tratamiento , Vimentina/análisisRESUMEN
Balloon cells are histopathological hallmarks of various cortical malformations, i.e., focal cortical dysplasia (Taylor's type, FCD IIb), hemimegalencephaly (HME) or cortical tubers (tuberous sclerosis, TSC). Whether this intriguing cell type results from similar pathogenetic pathways remains to be shown. Here, we analyzed the immunohistochemical distribution pattern of the CD34 epitope in surgical specimens from 34 patients with FCD IIb, compared to that of 6 patients with TSC and 3 patients with HME. In normal brain, CD34 occurs only transiently during neurulation, but cannot be detected in mature neuroectodermal cell progenies. In contrast, 58% of our patients showed CD34 immunoreactivity within a subpopulation of balloon cells. Interestingly, CD34-positive balloon cells were confined to the white matter, but never observed in neocortical layers. Furthermore, balloon cells expressing neurofilament protein were also restricted to white matter, whereas GFAP-positive balloon cells were observed either in white or gray matter location. Clinical characteristics did not significantly differ between patients with CD34-positive versus CD34-negative lesions. No significant correlation was found between CD34 expression and genetic alterations of the TSC1 gene, which is affected in many FCD and TSC patients and which plays a role in the regulation of cell size. Further studies are warranted to clarify the restricted expression of CD34 in balloon cells of the white matter.
Asunto(s)
Antígenos CD34/metabolismo , Encefalopatías/metabolismo , Encefalopatías/patología , Corteza Cerebral/anomalías , Adolescente , Encefalopatías/genética , Corteza Cerebral/metabolismo , Niño , Preescolar , Genotipo , Humanos , Inmunohistoquímica , Lactante , Recién Nacido , Pérdida de Heterocigocidad , Fenotipo , Convulsiones/etiología , Esclerosis Tuberosa/genética , Esclerosis Tuberosa/metabolismo , Esclerosis Tuberosa/patologíaRESUMEN
PURPOSE: To determine whether dysembryoplastic neuroepithelial tumors (DNTs) that belong to the neuronal migration disorders (NMDs) are to be classified with them or with "other low-grade brain tumors" regarding several etiologic, clinical, magnetic resonance imaging (MRI), and EEG findings. METHODS: These findings of 21 DNT patients were compared with those of 13 consecutive patients with other low-grade brain tumors and 41 NMD patients. RESULTS: The result is absolutely clear: nearly all findings in DNT patients (complications during pregnancy, birth, the newborn period and the postnatal period, age at first seizure, epileptic syndrome, seizure type, febrile seizures, retarded milestones, intellectual and neurologic deficits, MRI, interictal and ictal EEG findings) being similar or in agreement with those of other low-grade brain tumors, not with those of other NMDs. CONCLUSIONS: Regarding various clinical features including surgery outcome, MRI, and EEG findings, DNTs should be classified with the other low-grade brain tumors, rather than with NMDs.
Asunto(s)
Neoplasias Encefálicas/complicaciones , Encéfalo/anomalías , Epilepsia/etiología , Epilepsia/cirugía , Neoplasias Neuroepiteliales/complicaciones , Teratoma/complicaciones , Adolescente , Adulto , Neoplasias Encefálicas/clasificación , Niño , Preescolar , Electroencefalografía , Epilepsia/diagnóstico , Humanos , Imagen por Resonancia Magnética , Neoplasias Neuroepiteliales/clasificación , Teratoma/clasificaciónRESUMEN
Focal cortical dysplasia (FCD) is characterized by a localized malformation of the neocortex and underlying white matter. Balloon cells, similar to those observed in tuberous sclerosis, are present in many cases (FCD(bc)). In these patients, a hyperintense funnel-shaped subcortical lesion tapering toward the lateral ventricle was the characteristic finding on fluid-attenuated inversion recovery magnetic resonance imaging scans. Surgical lesionectomy results in complete seizure relief. Although the pathogenesis of FCD(bc) remains uncertain, histopathological similarities indicate that FCD(bc) may be related pathogenetically to tuberous sclerosis. Here, we studied alterations of the TSC1 and TSC2 genes in a cohort of patients with chronic, focal epilepsy and histologically documented FCD(bc) (n = 48). DNA was obtained after microdissection and laser-assisted isolation of balloon cells, dysplastic neurons, and nonlesional cells from adjacent normal brain tissue. Sequence alterations resulting in amino acid exchange of the TSC1 gene product affecting exons 5 and 17 and silent base exchanges in exons 14 and 22 were increased in patients with FCD(bc) compared with 200 control individuals (exon 5, 2.3% FCD(bc) vs 0% C; exon 17, 35% FCD(bc) vs 1.0% C; exon 14, 37.8% FCD(bc) vs 15% C; exon 22, 45% FCD(bc) vs 23.8% C). Sequence alterations could be detected in FCD(bc) and in adjacent normal cells. In 24 patients, DNA was suitable to study loss of heterozygosity at the TSC1 gene locus in microdissected FCD(bc) samples compared with control tissue. Eleven FCD(bc) cases exhibited loss of heterozygosity. In the TSC2 gene, only silent polymorphisms were detected at similar frequencies as in controls. Our findings indicate that FCD(bc) constitutes a clinicopathological entity with distinct neuroradiological, neuropathological, and molecular genetic features. These data also suggest a role of the TSC1 gene in the development of FCD(bc) and point toward a pathogenic relationship between FCD(bc) and the tuberous sclerosis complex.
Asunto(s)
Neocórtex/patología , Proteínas/genética , Esclerosis Tuberosa/genética , Esclerosis Tuberosa/patología , Sustitución de Aminoácidos/genética , ADN/aislamiento & purificación , Análisis Mutacional de ADN , Disección , Epilepsia/genética , Epilepsia/patología , Epilepsia/cirugía , Humanos , Rayos Láser , Pérdida de Heterocigocidad/genética , Reacción en Cadena de la Polimerasa , Polimorfismo Genético/genética , Esclerosis Tuberosa/cirugía , Proteína 1 del Complejo de la Esclerosis Tuberosa , Proteínas Supresoras de TumorRESUMEN
Two infants with severe drug refractory focal epilepsy caused by Sturge-Weber syndrome and extensive cerebral leptomeningeal angiomatosis were referred for preoperative video-electroencephalographic evaluation. Brain imaging with computed tomography and gadolinium-enhanced magnetic resonance imaging demonstrated bilateral disease in both children with a predominance of involvement of one hemisphere. Clinical examination and neurophysiology with ictal video recording demonstrated epileptogenesis from one hemisphere. Successful surgical treatment with functional hemispherectomy was followed by good long-term seizure control in both patients. The dramatic seizure control was accompanied by markedly improved quality of life for the family and children. These cases indicate that the spectrum of children that may benefit from epilepsy surgery should not be viewed too restrictively, and subsets of children with localization related epilepsy caused by extensive lesions may be resective surgical candidates with a good seizure outcome prognosis.