Modifications of lung microbiota structure in traumatic brain injury ventilated patients according to time and enteral feeding formulas: a prospective randomized study.

BACKGROUND: Specialized diets enriched with immune nutrients could be an important supplement in patients (pts) with acute traumatic brain injury (TBI). Omega-3 and arginine may interact with immune response and microbiota. No data are available about the role of the specialized diets in modulating the lung microbiota, and little is known about the influence of lung microbiota structure in development of ventilator-associated pneumonia (VAP) in TBI pts. The aims of this study are to evaluate the impact of specific nutrients on the lung microbiota and the variation of lung microbiota in TBI pts developing VAP. METHODS: A cohort of 31 TBI pts requiring mechanical ventilation in ICU was randomized for treatment with specialized (16pts) or standard nutrition (15pts). Alpha and beta diversity of lung microbiota were analyzed from bronco Alveolar Lavage (BAL) samples collected at admission and 7 days post-ICU admission in both groups. A further analysis was carried out on the same samples retrospectively grouped in VAP or no VAP pts. RESULTS: None developed VAP in the first week. Thereafter, ten out of thirty-one pts developed VAP. The BAL microbiota on VAP group showed significant differences in beta diversity and Staphylococcus and Acinetobacter Genera were high. The specialized nutrition had influence on beta diversity that reached statistical significance only in Bray-Curtis distance. CONCLUSION: Our data suggest that TBI patients who developed VAP during ICU stay have different structures of BAL microbiota either at admission and at 7 days post-ICU admission, while no correlation has been observed between different enteral formulas and microbiota composition in terms of richness and evenness. These findings suggest that targeting the lung microbiota may be a promising approach for preventing infections in critically ill patients.

Wearable pulmonary monitoring system with integrated functional lung imaging and chest sound recording: a clinical investigation in healthy subjects.

Objective.Current wearable respiratory monitoring devices provide a basic assessment of the breathing pattern of the examined subjects. More complex monitoring is needed for healthcare applications in patients with lung diseases. A multi-sensor vest allowing continuous lung imaging by electrical impedance tomography (EIT) and auscultation at six chest locations was developed for such advanced application. The aims of our study were to determine the vest's capacity to record the intended bio-signals, its safety and the comfort of wearing in a first clinical investigation in healthy adult subjects.Approach.Twenty subjects (age range: 23-65 years) were studied while wearing the vests during a 14-step study protocol comprising phases of quiet and deep breathing, slow and forced full expiration manoeuvres, coughing, breath-holding in seated and three horizontal postures. EIT, chest sound and accelerometer signals were streamed to a tablet using a dedicated application and uploaded to a back-end server. The subjects filled in a questionnaire on the vest properties using a Likert scale.Main results.All subjects completed the full protocol. Good to excellent EIT waveforms and functional EIT images were obtained in 89% of the subjects. Breathing pattern and posture dependent changes in ventilation distribution were properly detected by EIT. Chest sounds were recorded in all subjects. Detection of audible heart sounds was feasible in 44%-67% of the subjects, depending on the sensor location. Accelerometry correctly identified the posture in all subjects. The vests were safe and their properties positively rated, thermal and tactile properties achieved the highest scores.Significance.The functionality and safety of the studied wearable multi-sensor vest and the high level of its acceptance by the study participants were confirmed. Availability of personalized vests might further advance its performance by improving the sensor-skin contact.

Biology and crop production in Space environments: Challenges and opportunities.

Long-term manned space-exploration missions and the permanence of human colonies on orbital stations or planetary habitats will require the regeneration of resources onboard or in-situ. Bioregenerative Life Support Systems (BLSSs) are artificial environments where different compartments, involving both living organisms and physical-chemical processes, are integrated to achieve a safe, self-regulating, and chemically balanced Earth-like environment to support human life. Higher plants are key elements of such systems and Space greenhouses represent the producers' compartment. Growing plants in Space requires the knowledge of their growth responses not only to all environmental factors acting on Earth, but also to specific Space constraints such as altered gravity, ionizing radiations and confined volume. Moreover, cultivation techniques need to be adjusted considering such limitations. The type and intensity of environmental factors to be taken into account depend on the mission scenarios. Here, we summarize constraints and opportunities of cultivating higher plants in Space to regenerate resources and produce fresh food onboard. Both biological and agro-technological issues are considered briefly going through experiments both ground-based on Earth and in Space.

An Ecological Studybased on Textile Sensory Platforms to Improve Safety and Well-Being at Work.

This paper describes a study performed in the frame of WEARABLES project and reports about preliminary analysis of the results on the activity, HR and breathing rate distribution. Objective of the study was the monitoring of employees' well-being finalized at the investigation on the correlation between daily working activity and the observed physical parameters. The study has been performed by using sensing textiles, to collect objective work-correlated parameters during daily activity aiming at the acquisition of objective indicators for an improved management of people within teams. Scope of the project was to monitor a sample of 28 volunteers in environmental service delivery (at the Amey's contract with Wolverhampton City Council), for a period of two non-consecutive weeks per volunteer, with a total of 275 data acquisition sessions.

A Pilot Study on an Integrated Service Based on Wearable Textile Platforms to promote Workers Wellness at Workplace.

This paper describes a study performed in the frame of Wearables project and reports preliminary results. Objective of the study was the implementation of an integrated service finalized to increase employees' well-being through the investigation on the correlation between daily working activity and the observed physical parameters. The project monitored 28 volunteers employed in the field of waste collection (at the Amey's contract with Wolverhampton City Council), for a total of 275 data acquisition sessions. The study has been performed using sensing textiles, to collect objective work-correlated parameters during daily activity, aiming at the acquisition of objective indicators for an improved wellbeing. Physical parameters like heart rate, energy expenditure and heart rate activity-zones distribution have been evaluated from data acquired during normal working activity. The service produced encouraging results both in terms of monitoring individual subjects and in identifying trends correlated to different roles or tasks covered by workers. Also in term of usability and acceptability the system showed interesting potentialities, proving how wearable technologies can trigger innovative approaches and open new prospective in the growing field of workplace wellness.

Dodecagonal plasmonic quasicrystals for phage-based biosensing.

In this work, we fabricate and characterize a novel sensitive two-dimensional surface enhanced Raman spectroscopy (SERS) substrate made of plasmonic nanocavities in a photonic quasicrystal arrangement characterized by a 12-fold rotational symmetry. Our SERS device is capable of detecting chemisorbed bacteriophages at a femtomolar range. Most importantly, the paper presents for the first time a study on the procedure to functionalize the plasmonic quasicrystal with bacteriophages of the Podoviridae family. The immobilization of the phages on the plasmonic substrate has been studied and verified through SERS measurements. A new stable peak, visible in the SERS spectra at 1326 cm-1 at a greater than 60 times amplification, confirms the immobilization of the phages on the substrate. This functionalization approach can be used also for other types of phages or plasmonic sensors and hence, our achievements could allow the development of novel systems for the specific detection of different species of bacteria.

Microbial Community Dynamics and Response to Plant Growth-Promoting Microorganisms in the Rhizosphere of Four Common Food Crops Cultivated in Hydroponics.

Plant growth promoting microorganisms (PGPMs) of the plant root zone microbiome have received limited attention in hydroponic cultivation systems. In the framework of a project aimed at the development of a biological life support system for manned missions in space, we investigated the effects of PGPMs on four common food crops (durum and bread wheat, potato and soybean) cultivated in recirculating hydroponic systems for a whole life cycle. Each crop was inoculated with a commercial PGPM mixture and the composition of the microbial communities associated with their root rhizosphere, rhizoplane/endosphere and with the recirculating nutrient solution was characterised through 16S- and ITS-targeted Illumina MiSeq sequencing. PGPM addition was shown to induce changes in the composition of these communities, though these changes varied both between crops and over time. Microbial communities of PGPM-treated plants were shown to be more stable over time. Though additional development is required, this study highlights the potential benefits that PGPMs may confer to plants grown in hydroponic systems, particularly when cultivated in extreme environments such as space.

First detection of papillomaviruses and polyomaviruses in swimming pool waters: unrecognized recreational water-related pathogens?

AIMS: Viral outbreaks associated with swimming pools have been described worldwide. The objective of this study was to examine the extent of viral contamination in indoor and outdoor swimming pools. METHODS AND RESULTS: Pools were examined for the presence of human enteric viruses (adenovirus, norovirus and enterovirus) and nonenteric viruses (papillomavirus and polyomavirus-BK, JC, KI, WU and Merkel cell). Bacteriological parameters were also evaluated. The analysed pool waters met microbiological quality standards. Enteric viruses were not detected. On the other hand, papillomaviruses (HPV8, 12, 23, 25, 120 and unclassified HPVs) and polyomaviruses (JC and Merkel cell polyomaviruses) were detected in 9/14 samples (64%). The number of HPV DNA copies in pool waters, measured by quantitative Real-time PCR, ranged from 1.27E+04 to 1.13E+05/10L. CONCLUSION: Results show that a variety of nonenteric viruses may be discharged in pool waters by various secretions and excretions from infected individuals or asymptomatic carriers. SIGNIFICANCE AND IMPACT OF THE STUDY: To the best of our knowledge, this is the first report on human papillomaviruses and polyomaviruses in swimming pools. The likelihood that these viruses can be transmitted by recreational activities deserves to be explored in future studies.

Preliminary evaluation of Wearable Wellness System for Obstructive Sleep Apnea detection.

Several studies have proven how sleep deprivation has a negative impact on daily life, affecting people's psychophysical state. In this field, research is focusing on the improvement of unobtrusive sleep monitoring devices for promoting sleep hygiene and early detection of sleep disorders. This study aims to assess the use of a textile-based wearable system, with its associated apnea detection algorithm, in monitoring of Obstructive Sleep Apnea Syndrome (OSAs). The system has been compared through the simultaneous acquisition of physiological signals in parallel with polysomnograph in laboratory and home environments. Results show that such a wearable system could be successfully used for early detection of OSAs (Obstructive Sleep Apnea Syndrome) and could stimulate people to a better self healthcare looking for a specialized medic examination and eventually undergoing to proper treatment avoiding the onset of OSAs co-morbidities.

An Italian investigation on non-tuberculous mycobacteria in an urban water supply.

BACKGROUND: Atypical or non-tuberculous mycobacteria (NTM) are considered opportunistic pathogenic bacteria. They are natural inhabitants of a broad variety of environmental reservoirs, including natural and treated waters. Due to the lack of Italian studies on these microorganisms in drinking water, an investigation was carried out on their occurrence and on species present in a municipal water distribution system in Rome. The presence of the traditional indicators of faecal contaminations and free living amoebae were also verified. METHODS: Two different methodological approaches based on cultural and molecular techniques have been applied in parallel. RESULTS: Mean concentrations of NTM obtained with the two analytical methodologies were roughly 102 CFU/L. Among the numerous NTM species identified, M. chelonae was the most frequently isolated. No correlation of NTM with indicators of faecal contamination and amoebae was found. CONCLUSIONS: Mycobacteria can be present even if routine tests show water to have an excellent hygienic quality.

Leaf anatomy and photochemical behaviour of Solanum lycopersicum L. plants from seeds irradiated with low-LET ionising radiation.

Plants can be exposed to ionising radiation not only in Space but also on Earth, due to specific technological applications or after nuclear disasters. The response of plants to ionising radiation depends on radiation quality/quantity and/or plant characteristics. In this paper, we analyse some growth traits, leaf anatomy, and ecophysiological features of plants of Solanum lycopersicum L. "Microtom" grown from seeds irradiated with increasing doses of X-rays (0.3, 10, 20, 50, and 100 Gy). Both juvenile and compound leaves from plants developed from irradiated and control seeds were analysed through light and epifluorescence microscopy. Digital image analysis allowed quantifying anatomical parameters to detect the occurrence of signs of structural damage. Fluorescence parameters and total photosynthetic pigment content were analysed to evaluate the functioning of the photosynthetic machinery. Radiation did not affect percentage and rate of seed germination. Plants from irradiated seeds accomplished the crop cycle and showed a more compact habitus. Dose-depended tendencies of variations occurred in phenolic content, while other leaf anatomical parameters did not show distinct trends after irradiation. The sporadic perturbations of leaf structure, observed during the vegetative phase, after high levels of radiation were not so severe as to induce any significant alterations in photosynthetic efficiency.

[Comparison of cultural methods for the recovery of Pseudomonas aeruginosa: the UNI EN ISO 16266 reference method and the alternative method Pseudalert®]. / Confronto di metodi colturali per il rilevamento di Pseudomonas aeruginosa: il metodo di riferimento UNI EN ISO 16266 e il metodo alternativo Pseudalert®.

BACKGROUND: For the water analysis, for Pseudomonas aeruginosa a presumptive positive result can be achieved in 40- 48 hours using the traditional membrane filtration technique followed by an additional 24-48 hour confirmation stage. Conversely, the Pseudalert Quanti-Tray™ method can give confirmed results after 24-28 hours. In this case, actively growing strains of Pseudomonas aeruginosa show a confirmed positive result when a specific enzyme cleaving the substrate in the reagent produces a blue fluorescence under 365 nm ultraviolet light. A comparison of the performance of the Pseudalert respect to the standard method was conducted using statistical methods. METHODS: Drinking water was analyzed in parallel with the membrane filtration technique using Pseudomonas CN agar (UNI EN ISO 16266) and the Pseudalert. Confirmation test are requested by the standard method and although Pseudalert Quanti-Tray™ gives confirmed results, all the positive isolates were also confirmed. Data were analyzed by statistical methods. RESULTS: For drinking water, Pseudalert showed a very high sensitivity (98,8%) and a high percentage of specificity (96,8%). From a total of 889 positive isolates, a very high confirmation rates (99,3%) was calculated. Statistical analyses confirmed that the two methods were not statistically different. CONCLUSIONS: These results indicate that the Pseudalert produces confirmed results in a shorter time than the standard reference method allowing the detection of Pseudomonas aeruginosa with no further confirmation steps. It could be a valid alternative method for the water analysis.

Microgravity effects on different stages of higher plant life cycle and completion of the seed-to-seed cycle.

Human inhabitation of Space requires the efficient realisation of crop cultivation in bioregenerative life-support systems (BLSS). It is well known that plants can grow under Space conditions; however, perturbations of many biological phenomena have been highlighted due to the effect of altered gravity and its possible interactions with other factors. The mechanisms priming plant responses to Space factors, as well as the consequences of such alterations on crop productivity, have not been completely elucidated. These perturbations can occur at different stages of plant life and are potentially responsible for failure of the completion of the seed-to-seed cycle. After brief consideration of the main constraints found in the most recent experiments aiming to produce seeds in Space, we focus on two developmental phases in which the plant life cycle can be interrupted more easily than in others also on Earth. The first regards seedling development and establishment; we discuss reasons for slow development at the seedling stage that often occurs under microgravity conditions and can reduce successful establishment. The second stage comprises gametogenesis and pollination; we focus on male gamete formation, also identifying potential constraints to subsequent fertilisation. We finally highlight how similar alterations at cytological level can not only be common to different processes occurring at different life stages, but can be primed by different stress factors; such alterations can be interpreted within the model of 'stress-induced morphogenic response' (SIMR). We conclude by suggesting that a systematic analysis of all growth and reproductive phases during the plant life cycle is needed to optimise resource use in plant-based BLSS.

Soilless cultivation of soybean for Bioregenerative Life-Support Systems: a literature review and the experience of the MELiSSA Project - Food characterisation Phase I.

Higher plants play a key role in Bioregenerative Life-Support Systems (BLSS) for long-term missions in space, by regenerating air through photosynthetic CO2 absorption and O2 emission, recovering water through transpiration and recycling waste products through mineral nutrition. In addition, plants could provide fresh food to integrate into the crew diet and help to preserve astronauts' wellbeing. The ESA programme Micro-Ecological Life-Support System Alternative (MELiSSA) aims to conceive an artificial bioregenerative ecosystem for resources regeneration, based on both microorganisms and higher plants. Soybean [Glycine max (L.) Merr.] is one of the four candidate species studied for soilless (hydroponic) cultivation in MELiSSA, because of the high nutritional value of the seeds. Within the MELiSSA programme - Food characterisation Phase I, the aim of the research carried out on soybean at the University of Naples was to select the most suitable European cultivars for cultivation in BLSS. In this context, a concise review on the state-of-the-art of soybean cultivation in space-oriented experiments and a summary of research activity for the preliminary theoretical selection and subsequent agronomical evaluation of four cultivars will be presented in this paper.

Daily-life monitoring of stroke survivors motor performance: the INTERACTION sensing system.

The objective of the INTERACTION Eu project is to develop and validate an unobtrusive and modular system for monitoring daily life activities, physical interactions with the environment and for training upper and lower extremity motor function in stroke subjects. This paper describes the development and preliminary testing of the project sensing platform made of sensing shirt, trousers, gloves and shoes. Modular prototypes were designed and built considering the minimal set of inertial, force and textile sensors that may enable an efficient monitoring of stroke patients. The single sensing elements are described and the results of their preliminary lab-level testing are reported.

Performances evaluation of piezoresistive fabric sensors as function of yarn structure.

This work focus on the characterization of piezoresistive fabric sensors, realized with conductive yarns that are similar in term of conductive components, but different only in term of geometry, the yarns have been realized according two different production processes while the sensors have been produced following the same process, fabric structure and same materials. The different geometry of the yarns affects dramatically conductivity and functionality of the sensors in term of sensitivity and hysteresis minimization. This result confirms that the functional components can be engineered during the different phases of the process production; to get new properties and new applications. Small changes at fibers level can be fundamental to improve the properties of the fabric sensors.

Performances evaluation of textile electrodes for EMG remote measurements.

This work focus on the evaluation of textile electrodes for EMG signals acquisition. Signals have been acquired simultaneously from textile electrode and from gold standard electrodes, by using the same acquisition system; tests were done across subjects and with multiple trials to enable a more complete analysis. This research activity was done in the frame of the European Project Interaction, aiming at the development of a system for a continuous daily-life monitoring of the functional performance of stroke survivors in their physical interaction with the environment.

Treatment of symptomatic lumbar spinal degenerative pathologies by means of combined conservative biochemical treatments.

Research in spine surgery has proposed new soft and less invasive techniques. These are the results of our experience with oxygen-ozone therapy, which we could experiment within the Italian National Health System over 3 years. A total of 1,920 patients were admitted on the basis of unselected enrolment because of lumbosciatic pain. Patients were divided into three groups: (A) Patients with degenerative disc disease and arthropathy: 509 (26.5%), (B) Patients with failed back surgery syndrome (FBSS): 1,027 (53.489%), and (C) Patients with pure herniated lumbar disc: 384 (20%). The rationale of the treatment for all these different pathologies we have taken into consideration is the biochemical mechanism by which they can engender pain and dysfunction. Treatment for group A: paravertebral injection and phleboclysis (two cycles of 6 sessions, one each 3 days) +endoscopic neurolysis. Treatment for group B: paravertebral injection and phleboclysis (two cycles of 6 sessions, one each 3 days) + endoscopic neurolysis with intradiscal procedure (named percutaneous peridurodiscolysis). Treatment for group C: paravertebral injection (two cycles of 6 sessions, one each 3 days) + percutaneous discolysis.The perceived quality of result for this minimally invasive procedure makes oxygen-ozone therapy an interesting weapon in the hands of doctors. Furthermore, if the technique loses its clinical effectiveness, it can be repeated without harm for the patient, and costs for the health organization are notably very low, above all if compared to surgical procedures.We underline the need that this treatment should be performed in protected structures, in operative rooms, under anesthesiologic control, and in the hands of specialists.

Wearable monitoring systems for psychological and physiological state assessment in a naturalistic environment.

Wearable monitoring systems based on Smart Fibers and Interactive Textile (SFIT) platforms combine imperceptible sensing and computing functions with an interactive communication network. The integration into clothes of bio-potential sensors for health monitoring provides daily physiological parameters through a continuous, personalized, self-made detection of vital signs and the tracking of behavioral indicators of the subject. SFIT platforms can be used unobtrusively into the routinely daily activity to perform remote monitoring of persons in different circumstances and situations: during controlled exercises and diagnostic procedures as a biofeedback tool, during the usual daily life, during sleep or even to monitor behavioral indexes and mood disorders. Treatment of stress may include also training in cognitive-behavioral skills. Moreover, physiological signs and behavioral monitoring based on a multivariable approach leads to an enhanced sensitivity and specificity of these systems for the prediction of critical events. This paper presents two applications: a platform used in the frame of PSYCHE project, based on textile platforms and portable sensing devices for the long term and short term acquisition of data from patients affected by mood disorders and a platform addressing healthy subjects, based on biofeedback methodology, designed for the training of professional drivers named Mental Bio.

Textile electrodes and integrated smart textile for reliable biomonitoring.

Since birth the first and the most natural interface for the body is fabric, a soft, warm and reassuring material. Cloth is usually covering more than 80 % of the skin; which leads us to consider textile material as the most appropriate interface where new sensorial and interactive functions can be implemented. The new generation of personalised monitoring systems is based on this paradigm: functions like sensing, transmission and elaboration are implementable in the materials through the textile technology. Functional yarns and fibres are usable to realise garments where electrical and computing properties are combined with the traditional mechanical characteristics, giving rise to textile platforms that are comparable with the cloths that are normally used to produce our garments. The feel of the fabric is the same, but the functionality is augmented. Nowadays, consumers demand user-friendly connectivity and interactivity; sensing clothes are the most natural and ordinary interface able to follow us, everywhere in a non-intrusive way, in natural harmony with our body.