RESUMEN
This study investigates the effects of dentin's drying time, roughness, and curing modes of resin cement on bond strength. Forty human teeth were divided into eight groups based on three experimental factors: dentin's roughness by 240-or 600-grit SiC paper (coarse or fine), dentin wetness with air-drying time (5-s or 10-s), and Single Bond Universal adhesive's curing mode by co-curing with RelyX Ultimate cement or light-curing separately (co-curing or light-curing). The micro-tensile bond strength of fifteen resin-dentin stikcs per groups was measured. Failure mode and adhesive layers were observed using stereoscopic and confocal laser scanning microscopy, respectively. The curing mode of the adhesive layer affected the bond strength of the dentin-resin cement (p<0.05). In particular, the light-curing mode exhibited a significantly higher bond strength than the co-curing one (p<0.05). The bond strength between the resin cement and dentin was improved in the 5-s drying groups than in the 10-s drying groups.
Asunto(s)
Recubrimiento Dental Adhesivo , Recubrimientos Dentinarios , Dentina , Ensayo de Materiales , Cementos de Resina , Propiedades de Superficie , Resistencia a la Tracción , Humanos , Cementos de Resina/química , Recubrimiento Dental Adhesivo/métodos , Recubrimientos Dentinarios/química , Microscopía Confocal , Bisfenol A Glicidil Metacrilato/química , Análisis del Estrés Dental , Curación por Luz de Adhesivos Dentales , Factores de TiempoRESUMEN
Resin-based composite materials are commonly used for restorations, but their dimensional changes during the polymerization could cause various clinical problems. This study evaluated the influence of a base of different materials and thicknesses on the stress magnitude and distribution in a second maxillary premolar with an MOD resin composite restoration using three-dimensional finite element analysis. A sound tooth without cavity was considered as the control group (ST), and another group was restored with composite resin without applying a base material in a MOD cavity (CR). The other three groups were restored with composite resin along with the following base materials: glass ionomer cement, low-viscosity resin, and tricalcium silicate, respectively (CR-GIC, CR-LR, and CR-TS). These three groups were further divided into two subgroups according to the thickness of the base layer: thin (0.5 mm) and thick (1.0 mm). The stress distribution was compared using the maximum principal stress after polymerization shrinkage and vertical loading with 600 N on the occlusal surface. Group ST showed the lowest stress value, and its stress propagation was confined to outer enamel surfaces only. Group CR demonstrated the highest stress distribution in the tooth-restoration interface with increased failure risk on marginal areas. The thin and thick subgroups of the three groups with a base layer had lower stress levels than Group CR. The base materials reduced the marginal stress caused by polymerization shrinkage of composite resin in MOD cavities. Different base materials and thicknesses did not affect the stress distribution.
RESUMEN
Background/purpose: One of the challenges in adhesive ceramic restorations is deterioration of tooth-cement-ceramic interfaces. This study was to quantitatively investigate lipopolysaccharide (LPS) penetration through adhesive ceramic restorations. Materials and methods: Standardized holes were formed on ceramic (IPS Empress CAD, Ivoclar Vivadent) and human dentin discs. Prepared discs were randomly assigned to experimental groups (n = 6/group): SC, self-adhesive resin cement (SmartCem 2, Dentsply Sirona) bonded to ceramic discs; ST, self-adhesive resin cement bonded to dentin discs; VC, etch-and-rinse resin cement (Variolink II, Ivoclar Vivadent AG) bonded to ceramic discs; VT, etch-and-rinse resin cement bonded to dentin discs. The specimens underwent thermocycling (10,000 cycles; 5-55 °C) then LPS penetration test until 5-weeks. A mixed effect analysis using R statistical language was performed for data analysis. Results: Dentin and ceramic bonded with etch-and-rinse resin cements (groups VT and VC) showed significantly less penetration than those with self-adhesive resin cement (groups ST and SC) (P < 0.05). ST showed significantly lower penetration than SC (P < 0.05), that showed similar penetration with positive control (P > 0.05). VC showed minimal penetration during observed time of period (P < 0.05). Scanning electron microscope observations showed different interfacial characteristics among the groups. Conclusion: Within the limitation of this study, etch-and-rinse resin cement showed less LPS penetration than self-adhesive resin cement on both ceramic and dentin surfaces.
RESUMEN
INTRODUCTION: The aim of this case report was to discuss the clinical application using a quantitative light-induced fluorescent (QLF) device for the diagnosis and treatment of a cracked tooth as visualizing the tooth's crack. CASE REPORT: A 39-year-old woman visited a dental hospital complaining of throbbing pain on tooth #36. Observation of the tooth with naked eyes showed that tooth had a ceramic restoration with no specific findings. QLF device's images around the restoration, and after removal of the restoration were taken. In the QLF image after removal of the restoration, clear red-fluorescent crack line was shown, indicating that microorganism had penetrated along the crack path. During root canal treatment, the QLF image of the inside of the pulp chamber showed a pattern in which the crack line progressed into the tooth. During the treatment of the cracked tooth, the crack line was removed as much as possible using the fluorescent information obtained by the QLF device, and crack lines of fluorescence images quantitatively analyzed using a QLF's software. CONCLUSION: Images acquired with the QLF device can provide useful information for detecting crack lines, recording the treatment process, and restorative management of cracked teeth.
Asunto(s)
Síndrome de Diente Fisurado , Fotoquimioterapia , Diente , Femenino , Humanos , Adulto , Fotoquimioterapia/métodos , Fármacos Fotosensibilizantes , Tratamiento del Conducto Radicular , ColorantesRESUMEN
BACKGROUND: The accuracy of intraoral scanning plays a crucial role in the workflow of computer-assisted design/computer-assisted manufacturing. However, data regarding scanning accuracy for inlay preparation designs are lacking. The purpose of this in vitro study was to evaluate the influence of the depth of the occlusal cavity and width of the gingival floor of the proximal box on the trueness and precision of intraoral scans for inlay restoration. METHODS: Artificial teeth were used in this study. Four types of preparations for mesio-occlusal inlay were performed on each #36 artificial tooth depending on two different depths of the occlusal cavity (1 mm and 2 mm) and widths of the gingival floor of the proximal box (1.5 mm and 2.5 mm). Artificial teeth were scanned 10 times each with Cerec Primescan AC, and another scan was performed subsequently with a laboratory scanner as a reference (n = 10). Standard tessellation language files were analyzed using a three-dimensional analysis software program. Experimental data were analyzed using two-way analysis of variance and the Bonferroni multiple comparison test. RESULTS: The narrow shallow group had significantly higher deviation values for trueness than the wide deep group (p < 0.05). The wide deep group had the lowest average deviation value for trueness and there was no significant difference between the narrow deep and wide shallow groups (p > 0.05). For the mean maximum positive deviation, the wide groups had significantly lower values than the narrow groups (p < 0.05). Trueness was affected by both the width and depth(p < 0.05), whereas the mean maximum positive deviation was affected by the width (p < 0.05). The mean maximum negative deviation was affected by all three factors (p < 0.05). Precision was affected by the depth and the interaction between the depth of the occlusal cavity and width of the gingival floor (p < 0.05). CONCLUSIONS: The design of different inlay cavity configurations affected the accuracy of the digital intraoral scanner. The highest average deviation for trueness was observed in the narrow shallow group and the lowest in the wide deep group. With regard to precision, the narrow shallow group showed the lowest average deviation, and the narrow deep group showed highest value.
Asunto(s)
Caries Dental , Incrustaciones , Humanos , Diseño Asistido por Computadora , Atención Odontológica , EncíaRESUMEN
AIM: This study aimed to evaluate the intaglio surface trueness and fit of zirconia crowns depending on the different machining strategies used with the CEREC system. MATERIALS AND METHODS: Thirty duplicate tooth models for a single zirconia crown were randomly assigned to the three groups (n = 10) according to the machining mode used for fabrication: grinding, wet milling, and dry milling. The scan data of the final crowns were compared to their design data to evaluate the intaglio surface trueness. The marginal and internal fits were evaluated using the cross-sectional method. The time required for the machining and sintering processes was measured for each group. RESULT: The wet milling group showed better trueness (RMS, 13.8 ± 1.0 µm) than the grinding and dry milling groups (p < 0.001). The marginal gap was greater in the grinding group (58.6 ± 28.9 µm) than that in the wet milling and dry milling groups (p < 0.001). The dry milling group required the shortest time for the manufacturing process. CONCLUSION: All machining modes fabricated crowns with a clinically acceptable trueness and fit. However, the dry milling mode was advantageous for the chairside CAD/CAM system with respect to time efficiency.
RESUMEN
PURPOSE: To test the feasibility of nitrogen-doped TiO2 nanoparticles in the killing of Streptococcus mutans (S. mutans) for short term treatment. METHODS: For the study, S. mutans were treated with the combinations of N-TiO2, visible light, and without/with 0.5% H2O2 inclusion. Visible light was irradiated for 3 minutes one time. RESULTS: Methylene blue solution was degraded (bleached) 5-30% by one of N-TiO2 (or TiO2) + visible laser (405 or 660 nm) +0.5% H2O2 conditions owing to almost linearly producing free radicals through photocatalysis. Antibacterial outcomes treated with N-TiO2 were slightly better than those by TiO2 regardless of test condition. Also, killing of S. mutans treated with 405 nm laser was slightly better than those by 660 nm laser. CLINICAL SIGNIFICANCE: S. mutans can be eliminated using N-TiO2 with clinically acceptable light (wavelength, intensity) and low concentration H2O2 condition under short term treatment.
Asunto(s)
Peróxido de Hidrógeno , Streptococcus mutans , Peróxido de Hidrógeno/farmacología , Titanio/farmacología , Luz , Antibacterianos/farmacologíaRESUMEN
PURPOSE: To test the efficacy of nitrogen doped-TiO2 (N-TiO2) nanoparticles (NPs) on teeth whitening under visible light irradiation. METHODS: N-TiOV2 NPs were prepared by the sol-gel method, using TiN as a precursor. Their light absorbance and crystal structures were characterized. Photocatalytic reactions were tested using methylene blue (MB) and extracted teeth. For the extracted teeth, carbomer gel, without or with 3% H2O2, and light irradiated, with subsequent evaluation of the color differences. RESULTS: Unlike ordinary TiO2, N-TiO2 showed high absorbance after 400 nm. N-TiO2 prepared with TiN as a precursor showed rutile phase over the TiN structure. For MB solution, N-TiO2 with 3% H2O2 showed the maximum decrease in absorbance after laser irradiation. Observing the effect on teeth, N-TiO2+3% H2O2+405 nm laser treatment achieved approximately 25% higher whitening than that by 15% H2O2 during the same treatment time. Higher H2O2 concentrations may offer faster results. CLINICAL SIGNIFICANCE: N-TiO2 nanoparticles (without or with 3% H2O2) show better whitening of teeth as compared to 15% H2O2, if used with a visible laser for 5 hours. The potential on N-TiO2 nanoparticles to be used as a tooth whitener needs to be further explored to reduce its application time.
Asunto(s)
Peróxido de Hidrógeno , Blanqueamiento de Dientes , Catálisis , Luz , Azul de Metileno/química , Nitrógeno/química , TitanioRESUMEN
PURPOSE: To compare the autofluorescence (AF) spectra of resin products with teeth to determine if this type of non-invasive testing is feasible for differentiating resin products from teeth during resin repair. METHODS: For the study, 11 methacrylate-based resin products were chosen. A 405 nm laser was used to induce AF, and a spectrophotometer and a qualitative laser-induced fluorescence (QLF) camera were used to obtain AF spectra and images, respectively. RESULTS: Resin products and teeth showed one or two emission peak(s) at 435-465 nm and 475-480 nm, respectively. Other resin constituents produced weak emission peaks beyond the 435-475 nm range. Resin products with high emission intensities produced bright images. When layered, surface resins (0.2 mm-thick) were different from underlying base resins and teeth. CLINICAL SIGNIFICANCE: During resin repair, a restored resin can be readily removed if AF spectroscopy is used alone or in combination with QLF imaging.
Asunto(s)
Resinas Compuestas , Diente , Resinas Compuestas/química , Restauración Dental Permanente/métodos , Metacrilatos/químicaRESUMEN
PURPOSE: To test the applicability of autofluorescence (AF) spectrum and image in the detection and identification of oral pathogens. METHODS: Oral pathogens (Candida albicans, Porphyromonas gingivalis, Streptococcus mutans) and teeth were used. To induce AF, the 405 nm laser was used as a light source, and AF was obtained and observed using a spectrometer, fluorescence camera, and microscope, respectively. RESULTS: The tested oral pathogens had similar spectral distributions, but their peak intensities and peak ratios were different. Their peak positions and spectral patterns were different from those of the tested sound and carious teeth. These differences were also found from the other referenced oral mucosa. Fluorescence image could localize the existence of oral bacteria. Oral pathogens could be imaged by fluorescence, but identification of each pathogen by image was not probable. CLINICAL SIGNIFICANCE: Oral pathogens can be observed and identified from the lesion if autofluorescence spectrum and fluorescence images are combined.
Asunto(s)
Caries Dental , Diente , Caries Dental/diagnóstico , Caries Dental/microbiología , Fluorescencia , Humanos , Rayos Láser , Streptococcus mutans , Diente/patologíaRESUMEN
This study aimed to measure real-time temperature changes in gold-restored teeth compared with intact teeth during the intake of hot and cold drinks. Sixteen molars, including eight natural intact teeth and eight restored teeth with gold inlays, were selected from the participants. Custom-made thermocouple sensors were attached to the coronal third of the buccal surface of teeth. Participants consecutively consumed hot and cold drinks according to a standardized regimen. Resting, maximum, and minimum temperatures; time to reach peak temperatures; and heating and cooling velocities were obtained. Statistical analysis was performed using independent two-sample t-test. Teeth with gold restorations showed a significantly higher maximum temperature (44.7 °C [SD 2.9]) than did natural teeth (40.5 °C [SD 1.2]) during hot water drinking and showed a lower minimum temperature (25.0 °C [SD 4.9]) than did natural teeth (31.5 °C [SD 3.1]) during cold water drinking. The heating and cooling rates for the teeth with gold restorations were two and three times higher than those of the natural teeth. Gold-restored teeth showed greater temperature change than intact teeth in terms of magnitude and velocity in response to temperature changes induced by hot and cold drinks.
Asunto(s)
Oro , Diente , Frío , Calor , Humanos , Diente Molar , Temperatura , Diente/fisiología , AguaRESUMEN
This study aimed to evaluate the stress distribution that results from temperature changes in teeth restored with gold inlay and to predict the fatigue life of the teeth. A thermal load regimen for finite element analysis (FEA) was established in an in vivo experiment, in which participants with gold inlays drank hot and cold water. An extracted human maxillary molar was converted into a 3D FEA model. The tooth models with Class I and II preparations were restored with gold inlay in the experimental groups. The stress distribution was analyzed under the thermal loads. Also, lifetime prediction was conducted using Basquin's formulation. The gold inlay groups showed a higher stress distribution than the preparation-only groups regardless of the type of preparation. The Class II gold inlay group showed the shortest fatigue life of 2.48×1011 cycles, whereas the Class I preparation group showed the longest fatigue life of 4.49×1016 cycles.
Asunto(s)
Porcelana Dental , Incrustaciones , Resinas Compuestas , Análisis del Estrés Dental , Fatiga , Análisis de Elementos Finitos , Oro , Humanos , Estrés MecánicoRESUMEN
PURPOSE: To investigate the effect of roughness and drying time of dentin as well as the number of coats of a self-adhesive composite-cement primer on the bond strength of self-adhesive composite cement. MATERIAL AND METHODS: Sixty human teeth were prepared and assigned to 12 groups (n = 5), according to three experimental factors: 1) dentin surface roughness, rough or fine, as achieved by 250- and 600-grit silicon carbide papers, respectively; 2) dentin wetness based on air-drying time (5 or 10 s); and 3) the self-adhesive composite-cement primer applications (no-coat, 1-coat, and 2-coat). Composite resin blocks were made with hybrid composite resin (M1 GraceFil) and cemented with G-CEM ONE (both GC). Cement-dentin sticks (12) were prepared, and the microtensile bond strength (µTBS) test was performed. Failure modes were observed with a stereomicroscope (40X), and bonding interfaces were evaluated with confocal laser scanning microscopy (CLSM). Statistical analysis was performed using three-way ANOVA and Tukey's post-hoc comparisons test (α = 0.05). RESULTS: Dentin roughness (250-grit > 600-grit, p = 0.000), drying time (5-s drying > 10-s drying, p = 0.000), and primer application (no-coat < 1-coat = 2-coat, p = 0.000) had significant effects on bond strength. These factors also showed significant interactions with each other (p = 0.003). The highest µTBS (31.8 ± 3.1 MPa) was observed in the 1-coat/fine roughness/10-s drying group and the lowest µTBS (13.4 ± 2.7 MPa) in the no-coat/coarse roughness/5-s drying group. CLSM showed higher penetration of cement in the primer-coated groups compared to that in the no-coat groups. CONCLUSION: Bond strength between the self-adhesive composite cement and dentin was higher in the fine-roughness dentin group than in the coarse-roughness dentin group, and in the 5-s drying group compared to the 10-s drying group. Applying a primer to dentin improved bond strength of the self-adhesive composite cement.
Asunto(s)
Recubrimiento Dental Adhesivo , Cementos de Resina , Resinas Compuestas/química , Cementos Dentales , Materiales Dentales/química , Dentina , Recubrimientos Dentinarios/química , Cementos de Ionómero Vítreo/química , Humanos , Ensayo de Materiales , Cementos de Resina/química , Propiedades de Superficie , Resistencia a la TracciónRESUMEN
STATEMENT OF PROBLEM: Clinical guidelines for obtaining accurate scan data during the intraoral scanning of inlay cavities with various configurations and interproximal distances are lacking. PURPOSE: The purpose of this in vitro study was to evaluate the effect of interproximal distance and cavity type on the accuracy of digital scans for inlay cavities, including proximal cavities. MATERIAL AND METHODS: Four artificial teeth with 4 types of inlay cavities designed based on the buccolingual width and gingival level of the proximal box were installed in a mannequin at distances of 0.6, 0.8, and 1.0 mm from the adjacent teeth. Reference scans of the 4 artificial teeth were obtained by using a laboratory scanner. The CEREC Primescan AC was used to acquire digital scan data (each n=10). Standard tessellation language (STL) files were analyzed with a 3-dimensional analysis software program. The mean deviation values were measured with a 3-dimensional best-fit alignment method to evaluate the accuracy of the digital scan data. Statistical analyses were performed by using 2-way ANOVA and the Bonferroni multiple comparison test (α=.05). RESULTS: As per the interproximal distance, the 1.0-mm group showed significantly higher trueness than the 0.6-mm group (P<.05). As the interproximal distance increased, the maximum positive deviation significantly decreased (P<.05). Maximum negative deviation and precision of the scan data were not significantly different among the distance groups (P>.05). Cavity type had a significant influence on the trueness and precision of the scan data (P<.05). In particular, the narrow long cavity type had an adverse effect on the precision and maximum positive deviation of scan data. CONCLUSIONS: During the intraoral scanning of class II inlay restoration, interproximal distance and cavity type affected the accuracy of an intraoral scan. As the interproximal distance increased, the trueness of the acquired digital images increased and the maximum positive deviation significantly decreased. The narrow long cavity type negatively affected the mean maximum positive deviation and precision of scan data.
Asunto(s)
Técnica de Impresión Dental , Modelos Dentales , Diseño Asistido por Computadora , Incrustaciones , Imagenología TridimensionalRESUMEN
OBJECTIVE: This study aimed to evaluate the influence of the presence of adjacent teeth on the accuracy of intraoral scanning (IOS) systems for class II inlay preparation. MATERIALS AND METHODS: The mesio-occlusal inlay preparation was prepared in an anatomical model of the maxillary molar. The prepared tooth was secured to a typodont with the mesial adjacent tooth removed or in situ. Ten digital impressions of the inlay preparation were acquired using three IOS systems (CEREC Primescan, 3Shape TRIOS 3, and Medit i500). A laboratory scanner (3Shape E3) was used to obtain the reference scan data. The mean absolute deviation values were calculated to evaluate the accuracy of the digital models. RESULTS: The group with the adjacent teeth present showed lower trueness and precision compared to that without the adjacent tooth (p < .05). Significant differences were observed among the IOS systems (p < .05). Primescan showed the highest accuracy, irrespective of the presence of adjacent teeth. CONCLUSIONS: The presence of the adjacent tooth negatively affected the accuracy of all the IOSs tested. Although the performance of Primescan was superior to that of TRIOS 3 and i500, each IOS system showed clinically acceptable levels of accuracy for class II inlay preparation. CLINICAL SIGNIFICANCE: The adjacent tooth can be a confounding factor for accurate digital impressions of class II inlay preparation.
Asunto(s)
Técnica de Impresión Dental , Modelos Dentales , Diseño Asistido por Computadora , Arco Dental , Imagenología Tridimensional , IncrustacionesRESUMEN
The treatment of teeth with severely calcified canals is a difficult procedure for clinicians. Procedural accidents such as drill path deviation or perforation of the root canal often occur. 3D-printed directional guides made using CBCT and CAD/CAM can help the clinician to accurately drill in the original root canal direction. Furthermore, chairside operating times and excessive loss of tooth structure are reduced, and the risk of perforation is avoided. The present case report describes two cases of calcified root canals with path deviations that were retreated utilizing a guided endodontic technique.
Asunto(s)
Tomografía Computarizada de Haz Cónico , Cavidad Pulpar , Humanos , Impresión Tridimensional , Retratamiento , Tratamiento del Conducto RadicularRESUMEN
Diagnosing a cracked tooth is a challenge for dental clinicians. This report describes the use of a quantitative light-induced fluorescent (QLF) device that detects fluorescence reactions with visible light (405 nm) to visually identify microscopic tooth cracks during the diagnosis and treatment of cracked teeth that caused pulp disease. Fluorescence images of the occlusal surface, before and after removal of the restoration, and inside of the access cavity for root canal treatment were obtained using an intraoral capture-type QLF device (Q-ray penC; AIOBIO, Seoul, Korea). The device provided visual information such as enhanced magnification and fluorescent images to identify cracks on the exterior of the tooth, around restorations, and inside the cavity after removal of the restoration by a simple image capture process. The device was able to demonstrate the existence of the crack line and to predict the depth of cracks during treatment.The QLF device showed a potential benefit in the diagnosis and characterization, including the location and depth, of tooth cracks.
Asunto(s)
Síndrome de Diente Fisurado , Caries Dental , Fracturas de los Dientes , Diente , Síndrome de Diente Fisurado/diagnóstico , Síndrome de Diente Fisurado/terapia , Humanos , Tratamiento del Conducto RadicularRESUMEN
The purpose of this study was to investigate the effect of silane-containing universal adhesives on the bonding strength of lithium disilicate. Two-hundred-and-forty lithium disilicate blocks were divided into 16 groups according to the following surface treatments: hydrofluoric acid (HF)-treated or not, silane-treated or not, and the type of universal adhesive used (All-Bond Universal (ABU); Prime & bond (PB); Clearfil Universal Bond (CU); Single bond Universal (SBU)). After surface treatment, resin discs were bonded to each lithium disilicate using dual-cure resin cement. Bonded specimens were stored in distilled water for 24 h and then subjected to microtensile bond strength (µTBS) test. Failure modes were examined under stereomicroscope. Microscopic observation of bonded interfaces was analyzed using scanning electron microscopy. The µTBS data were statistically analyzed. Regardless of silane treatment, all groups treated with HF showed higher bonding strengths compared to those that were not treated with HF (p < 0.05). In groups treated with HF, the bonding strength increased after silane application (p < 0.05) except PB and CU (p > 0.05). Adhesive failures were dominant in all groups, but some mixed failures were observed in ABU treated with HF and silane. While most of the specimens that were not treated with silane after HF application only showed loose bonding between the ceramic and resin cement due to partial gaps, the specimens treated with silane application after HF showed a tight ceramic-resin interface. In conclusion, the silane in universal adhesives did not effectively improve the bonding strength between lithium disilicate and resin cement.
RESUMEN
Previous studies have shown that mesenchymal stem cells (MSCs) derived from various tissue sources can be differentiated into smooth muscle-like cells (SMLCs) in vitro. In this paper, dental pulp-derived mesenchymal stem cells (DPSCs) were evaluated for their differentiation ability towards smooth muscle-like cells (SMLCs) under the effect of widely used cytokines (TGF-ß1 and PDGF-BB) with special focus on different culturing environments. For this purpose, both the commercially used culturing plates (Norm-c) and 0.1% gelatin-precoated (Gel-c) plates were used. Isolated cells displayed plastic adherence, pluripotency and cell surface marker profiling, and adipogenic and osteogenic differentiation potential with lineage specific marker expression. Differentiated cells induced under different culturing plates showed successful differentiation into SMLCs by positively expressing smooth muscle cell (SMC) specific markers both at the mRNA and protein levels. Gelatin coating could substantially enhance DPSC differentiation potential than Norm-c-induced cells. However, the absence of mature marker MHY-11 by immunostaining results from all treatment groups further indicated the development of immature and synthetic SMLCs. Finally, it was concluded that DPSC differentiation ability into SMLCs can be enhanced under cytokine treatment as well as by altering the cellular niche by precoating the culturing plates with suitable substrates. However, to get fully functional, contractile, and mature SMLCs, still many different cytokine cocktail combinations and more suitable coating substrates will be needed.
Asunto(s)
Técnicas de Cultivo de Célula/instrumentación , Diferenciación Celular/efectos de los fármacos , Pulpa Dental/citología , Células Madre Mesenquimatosas/citología , Miocitos del Músculo Liso/citología , Becaplermina/farmacología , Biomarcadores/metabolismo , Técnicas de Cultivo de Célula/métodos , Diferenciación Celular/fisiología , Linaje de la Célula , Células Cultivadas , Colágeno , Medios de Cultivo/química , Medios de Cultivo/farmacología , Geles , Humanos , Células Madre Mesenquimatosas/efectos de los fármacos , Células Madre Mesenquimatosas/fisiología , Células Madre Pluripotentes/fisiología , Factor de Crecimiento Transformador beta1/farmacologíaRESUMEN
Purpose This study aimed to evaluate the influence of tooth location and inlay cavity type on the accuracy of intraoral digital impressions.Methods Class II inlay preparation was performed on anatomical models of the maxillary first molar (16) and mandibular first molar (46). Mesio-occlusal and disto-occlusal cavities were prepared, such that the axial wall of the proximal box measured 1 mm or 2 mm in height. Thus, four types of inlay cavities were prepared in 16 and 46, respectively. Ten digital impressions of each cavity were obtained using Cerec Primescan (Sirona). Reference scans were obtained with a laboratory scanner (E3, 3Shape). All scan data were exported for comparative analysis of the three-dimensional models. Mean absolute deviation values were calculated to evaluate the trueness and precision of the digital models. Color-coded maps were used for the qualitative analysis of deviations.Results The overall results showed that the trueness for 16 (10.43 ± 0.39 µm) was higher than that for 46 (12.42 ± 0.59 µm) (p < 0.05), while the precision was similar between 16 (3.08 ± 0.92 µm) and 46 (3.08 ± 0.76 µm). The cavity type affected the accuracy of the digital impressions. The highest deviation was observed in positive directions at the margins of the proximal boxes regardless of the cavity type.Conclusions Tooth location and cavity type affected the accuracy of intraoral digital impressions. Positive deviations were observed at the margins of the proximal boxes.