RESUMEN
Atomic force microscopy (AFM) is a powerful tool for analysing the shapes of individual molecules and the forces acting on them. AFM-based force spectroscopy provides insights into the structural and energetic dynamics of biomolecules by probing the interactions within individual molecules, or between a surface-bound molecule and a cantilever that carries a complementary binding partner. Here, we show that an AFM cantilever with an antibody tether can measure the distances between 5-methylcytidine bases in individual DNA strands with a resolution of 4 Å, thereby revealing the DNA methylation pattern, which has an important role in the epigenetic control of gene expression. The antibody is able to bind two 5-methylcytidine bases of a surface-immobilized DNA strand, and retracting the cantilever results in a unique rupture signature reflecting the spacing between two tagged bases. This nanomechanical approach might also allow related chemical patterns to be retrieved from biopolymers at the single-molecule level.
Asunto(s)
Citidina/análogos & derivados , Metilación de ADN , ADN de Cadena Simple/química , Nanotecnología/métodos , Citidina/química , Epigénesis Genética , Microscopía de Fuerza Atómica , Conformación de Ácido NucleicoRESUMEN
Two-dimensional, crystalline bacterial cell surface layers, termed S-layers, are one of the most commonly observed cell surface structures of prokaryotic organisms. In the present study, genetically modified S-layer protein SbpA of Bacillus sphaericus CCM 2177 carrying the short affinity peptide Strep-tag I or Strep-tag II at the C terminus was used to generate a 2D crystalline monomolecular protein lattice on a silicon surface. Because of the genetic modification, the 2D crystals were addressable via Strep-tag through streptavidin molecules. Atomic force microscopy (AFM) was used to investigate the topography of the single-molecules array and the functionality of the fused Strep-tags. In high-resolution imaging under near-physiological conditions, structural details such as protein alignment and spacing were resolved. By applying molecular recognition force microscopy, the Strep-tag moieties were proven to be fully functional and accessible. For this purpose, streptavidin molecules were tethered to AFM tips via approximately 8-nm-long flexible polyethylene glycol (PEG) linkers. These functionalized tips showed specific interactions with 2D protein crystals containing either the Strep-tag I or Strep-tag II, with similar energetic and kinetic behavior in both cases.
Asunto(s)
Glicoproteínas de Membrana/química , Microscopía de Fuerza Atómica/métodos , Cristalización , Polietilenglicoles/química , Silicio/química , Estreptavidina/química , Propiedades de SuperficieRESUMEN
BACKGROUND: The release of vesicles by red blood cells (RBCs) occurs in vivo and in vitro under various conditions. Vesiculation also takes place during RBC storage and results in the accumulation of vesicles in RBC units. The membrane protein composition of the storage-associated vesicles has not been studied in detail. The characterization of the vesicular membrane might hint at the underlying mechanism of the storage-associated changes in general and the vesiculation process in particular. STUDY DESIGN AND METHODS: Vesicles from RBCs that had been stored for various periods were isolated and RBCs of the same RBC units were used to generate calcium-induced microvesicles. These two vesicle types were compared with respect to their size with atomic force microscopy, their raft protein content with detergent-resistant membrane (DRM) analysis, and their thrombogenic potential and activity with annexin V binding and thrombin generation, respectively. RESULTS: The storage-associated vesicles and the calcium-induced microvesicles are similar in size, in thrombogenic activity, and in membrane protein composition. The major differences were the relative concentrations of the major integral DRM proteins. In storage-associated vesicles, stomatin is twofold enriched and flotillin-2 is threefold depleted. CONCLUSION: These data indicate that a stomatin-specific, raft-based process is involved in storage-associated vesiculation. A model of the vesiculation process in RBCs is proposed considering the raft-stabilizing properties of stomatin, the low storage temperature favoring raft aggregation, and the previously reported storage-associated changes in the cytoskeletal organization.
Asunto(s)
Vesículas Citoplasmáticas/metabolismo , Eritrocitos/metabolismo , Microdominios de Membrana/metabolismo , Proteínas de la Membrana/metabolismo , Adenosina Trifosfato/metabolismo , Algoritmos , Conservación de la Sangre/métodos , Eritrocitos/citología , Citometría de Flujo , Glucosa/metabolismo , Humanos , Concentración de Iones de Hidrógeno , Microscopía de Fuerza Atómica , Sodio/metabolismoRESUMEN
Plasmid DNA and viral RNA were imaged in a liquid environment by dynamic force microscopy (DFM) and fine structures of DNA with heights of 1.82+/-0.66 nm were obtained in topographical images. In simultaneously acquired phase images, DNA could be imaged with better contrast at lower imaging forces. By splitting the cantilever oscillation signal into lower and upper parts, the contribution of the adhesion between tip and sample to the topographical images was eliminated, resulting in better signal-to-noise ratio. DFM of the single stranded RNA genome of a human rhinovirus showed loops protruding from a condensed RNA core, 20-50 nm in height. The mechanical rigidity of the RNA was determined by single molecule pulling experiments. From fitting RNA stretching curves to the Worm-Like-Chain (WLC) model a persistence length of 1.0+/-0.17 nm was obtained.
Asunto(s)
Microscopía de Fuerza Atómica/métodos , Plásmidos/química , ARN Viral/química , Humanos , Níquel/química , Conformación de Ácido Nucleico , Plásmidos/análisis , ARN Viral/análisis , Rhinovirus/químicaRESUMEN
Semiquantitative prediction of the Uppsalator (electroosmotical oscillator developed in Uppsala) under constant voltage is confirmed via numerical solution of exact nonlinear integro-differential equations in partial derivatives (PDE). Critical voltage and characteristic features of pressure and liquid flow velocity as functions of time are reproduced with high precision. In particular, the number of inflection points per period of oscillating velocity increases with growing voltage from two to six. A new feature is demonstrated. For sufficiently high voltages the Uppsalator shows normal deceleration, i.e., the oscillation period grows with voltage. However, in some supercritical range of voltages, acceleration is observed, as expressed by decrease of the oscillation period with growing voltage. Thus, the Uppsalator at constant voltage considered earlier as an impossible phenomenon represents a great challenge for experimental verification.
Asunto(s)
Electroósmosis/instrumentación , Modelos Teóricos , Aceleración , DesaceleraciónRESUMEN
Dynamic force microscopy (DFM) was used to image human rhinovirus HRV2 alone and complexed with single receptor molecules under near physiological conditions. Specific and site-directed immobilization of HRV2 on a model cell membrane resulted in a crystalline arrangement of virus particles with hexagonal symmetry and 35 nm spacing. High-resolution imaging of the virus capsid revealed about 20 resolvable structural features with 3 nm diameters; this finding is in agreement with protrusions seen by cryo-electron microscopy. Binding of receptor molecules to individual virus particles was observed after injection of soluble receptors into the liquid cell. Virus-receptor complexes with zero, one, two, or three attached receptor molecules were resolved. The number of receptor molecules associated to virions increased over time. Occasionally, dissociation of single receptor molecules from viral particles was also observed.
Asunto(s)
Cápside/ultraestructura , Receptores Virales/ultraestructura , Rhinovirus/ultraestructura , Membrana Celular , Cristalografía , Humanos , Microscopía de Fuerza Atómica , Receptores Virales/fisiología , Rhinovirus/fisiologíaRESUMEN
Antibody binding to surface antigens in membranes is the primary event in the specific immune defence of vertebrates. Here we used force microscopy to study the dynamics of antibody recognition of mutant purple membranes from Halobacterium salinarum containing a genetically appended anti-Sendai recognition epitope. Ligation of individual anti-Sendai antibodies to their antigenic epitopes was observed over time. Their increase in number within a small selected area revealed an apparent kinetic on-rate. The membrane-bound antibodies showed many different conformations that ranged from globular to V- and Y-like shapes. The maximum distance of two Fab fragments of the same antibody was observed to be approximately 18 nm, indicating an overall strong intrinsic flexibility of the antibody hinge region. Fab fragments of bound anti-Sendai antibodies were allocated to antigenic sites of the purple membrane, allowing the identification and localization of individual recognition epitopes on the surface of purple membranes.
Asunto(s)
Anticuerpos Antivirales/ultraestructura , Mapeo Epitopo/métodos , Microscopía de Fuerza Atómica , Membrana Púrpura/inmunología , Anticuerpos Monoclonales/inmunología , Anticuerpos Monoclonales/ultraestructura , Anticuerpos Antivirales/inmunología , Antígenos de Superficie/genética , Antígenos de Superficie/inmunología , Sitios de Unión de Anticuerpos/inmunología , ARN Polimerasas Dirigidas por ADN/genética , ARN Polimerasas Dirigidas por ADN/inmunología , Halobacterium salinarum/genética , Halobacterium salinarum/inmunología , Fragmentos Fab de Inmunoglobulinas/inmunología , Fragmentos Fab de Inmunoglobulinas/ultraestructura , Cinética , Virus Sendai/inmunología , Tiempo , Proteínas Virales/genética , Proteínas Virales/inmunologíaRESUMEN
We employed magnetic ACmode atomic force microscopy (MACmode AFM) as a novel dynamic force microscopy method to image surfaces of biological membranes in their native environments. The lateral resolution achieved under optimized imaging conditions was in the nanometer range, even when the sample was only weakly attached to the support. Purple membranes (PM) from Halobacterium salinarum were used as a test standard for topographical imaging. The hexagonal arrangement of the bacteriorhodopsin trimers on the cytoplasmic side of PM was resolved with 1.5nm lateral accuracy, a resolution similar to images obtained in contact and tapping-mode AFM. Human rhinovirus 2 (HRV2) particles were attached to mica surfaces via nonspecific interactions. The capsid structure and 2nm sized protein loops of HRV2 were routinely obtained without any displacement of the virus. Globular and filamentous structures on living and fixed endothelial cells were observed with a resolution of 5-20nm. These examples show that MACmode AFM is a favorable method in studying the topography of soft and weakly attached biological samples with high resolution under physiological conditions.