1.
Anal Biochem
; 275(2): 187-91, 1999 Nov 15.
Artículo
en Inglés
| MEDLINE
| ID: mdl-10552903
RESUMEN
A rapid and simple HPLC assay has been developed to separate and quantify N-acyl glutamates and the corresponding carboxylic acids of the acyl moiety. This method was specifically developed to assay hydrolytic activity for glutamate carboxypeptidases. Although established assays for specific substrates of such enzymes exist, they may not be amenable for examining the hydrolytic activity of new substrate probes. This assay was developed to accommodate such probes.