RESUMEN
OBJECTIVE: To determine humoral responses to an equine encephalitis vaccine in healthy alpacas. DESIGN: Clinical trial. ANIMALS: 39 healthy alpacas on 1 farm and 86 healthy alpacas on a second farm. PROCEDURES: All alpacas were given 3 doses IM of a bivalent, killed-virus equine encephalitis vaccine, with 4 weeks between doses. Eastern equine encephalitis (EEE) virus neutralizing antibody responses were determined with a plaque reduction neutralization assay every 14 days in alpacas on the first farm and 70 days after the first dose of vaccine on the second farm. RESULTS: For alpacas on the first farm, geometric mean virus neutralizing antibody titer peaked 2 weeks after the third vaccine dose was given (ie, day 70). At this time, 29 of 38 (76%) animals were seropositive for antibodies against EEE virus, and percentage of animals
Asunto(s)
Anticuerpos Antivirales/biosíntesis , Formación de Anticuerpos , Camélidos del Nuevo Mundo/inmunología , Virus de la Encefalitis Equina del Este/inmunología , Encefalomielitis Equina Oriental/veterinaria , Vacunas Virales/inmunología , Animales , Anticuerpos Antivirales/sangre , Camélidos del Nuevo Mundo/sangre , Camélidos del Nuevo Mundo/virología , Relación Dosis-Respuesta Inmunológica , Encefalomielitis Equina Oriental/sangre , Encefalomielitis Equina Oriental/inmunología , Encefalomielitis Equina Oriental/prevención & control , Femenino , Inyecciones Intramusculares/veterinaria , Masculino , Vacunación/veterinaria , Vacunas de Productos Inactivados , Vacunas Virales/administración & dosificaciónRESUMEN
OBJECTIVE: To compare neutralizing antibody response between horses vaccinated against West Nile virus (WNV) and horses that survived naturally occurring infection. DESIGN: Cross-sectional observational study. ANIMALS: 187 horses vaccinated with a killed WNV vaccine and 37 horses with confirmed clinical WNV infection. PROCEDURE: Serum was collected from vaccinated horses prior to and 4 to 6 weeks after completion of an initial vaccination series (2 doses) and 5 to 7 months later. Serum was collected from affected horses 4 to 6 weeks after laboratory diagnosis of infection and 5 to 7 months after the first sample was obtained. The IgM capture ELISA, plaque reduction neutralization test (PRNT), and microtiter virus neutralization test were used. RESULTS: All affected horses had PRNT titers > or = 1:100 at 4 to 6 weeks after onset of disease, and 90% (18/20) maintained this titer for 5 to 7 months. After the second vaccination, 67% of vaccinated horses had PRNT titers > or = 1:100 and 14% had titers < 1:10. Five to 7 months later, 33% (28/84) of vaccinated horses had PRNT titers > or = 1:100, whereas 29% (24/84) had titers < 1:10. Vaccinated and clinically affected horses' end point titers had decreased by 5 to 7 months after vaccination. CONCLUSIONS AND CLINICAL RELEVANCE: A portion of horses vaccinated against WNV may respond poorly. Vaccination every 6 months may be indicated in certain horses and in areas of high vector activity. Other preventative methods such as mosquito control are warranted to prevent WNV infection in horses.
Asunto(s)
Anticuerpos Antivirales/biosíntesis , Enfermedades de los Caballos/inmunología , Caballos/inmunología , Vacunas Virales/inmunología , Fiebre del Nilo Occidental/veterinaria , Virus del Nilo Occidental/inmunología , Animales , Anticuerpos Antivirales/sangre , Estudios Transversales , Culicidae/virología , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Enfermedades de los Caballos/sangre , Enfermedades de los Caballos/prevención & control , Masculino , Control de Mosquitos , Pruebas de Neutralización/veterinaria , Ensayo de Placa Viral/veterinaria , Vacunas Virales/administración & dosificación , Fiebre del Nilo Occidental/sangre , Fiebre del Nilo Occidental/inmunología , Fiebre del Nilo Occidental/prevención & controlRESUMEN
West Nile virus (WNV) infection in 4 reindeer (Rangifer tarandus) resulted in lymphohistiocytic encephalomyelitis within the medulla oblongata and cervical spinal cord. Immunohistochemistry revealed WNV antigen within neurons and among mononuclear cell infiltrates. These represent the first known cases of clinical WNV infection in Cervidae. Clinical signs and lesions were similar to those described in horses. Nucleotide sequence of a 768-bp region of the WNV E-glycoprotein gene revealed 1 nucleotide mutation, which resulted in a single amino acid substitution from a serine to a glycine (position 227 of E-glycoprotein) when compared with the prototype WNV-NY99 strain (isolated from Bronx zoo flamingo 382-99).
Asunto(s)
Reno/virología , Fiebre del Nilo Occidental/veterinaria , Virus del Nilo Occidental/aislamiento & purificación , Animales , Anticuerpos Antivirales/sangre , Antígenos Virales/metabolismo , Encéfalo/patología , Encéfalo/virología , Iowa , Masculino , Nebraska , Neuronas/patología , Neuronas/virología , Pruebas de Neutralización/veterinaria , ARN Viral/química , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Médula Espinal/patología , Médula Espinal/virología , Fiebre del Nilo Occidental/sangre , Fiebre del Nilo Occidental/patología , Fiebre del Nilo Occidental/virología , Virus del Nilo Occidental/genéticaRESUMEN
In 2002, West Nile virus (WNV) infection with clinical neurologic disease and encephalomyelitis was described in reindeer (Rangifer tarandus). The susceptibility of reindeer to WNV prompted questions concerning vaccination of reindeer to prevent WNV infection. Between January and April 2003, eleven 2-4-yr-old, castrated male reindeer, some of which had antibody titers suggestive of prior exposure to WNV, were vaccinated three times at 4-wk intervals with a commercially available vaccine approved for use in horses. No adverse reactions to vaccination were noted. All vaccinated reindeer developed high neutralizing antibody titers to WNV, as determined by the plaque reduction neutralization test. Reindeer without antibody titers from previous natural exposure to WNV required a primary vaccination and one or two booster vaccinations for development of neutralizing antibody to WNV. Protective efficacy of vaccination was not evaluated. Vaccination of reindeer for WNV may be warranted in certain circumstances combined with management practices to limit exposure to potential vectors.
Asunto(s)
Anticuerpos Antivirales/sangre , Reno/inmunología , Vacunas Virales/inmunología , Fiebre del Nilo Occidental/veterinaria , Virus del Nilo Occidental/inmunología , Animales , Anticuerpos Antivirales/biosíntesis , Masculino , Pruebas de Neutralización/veterinaria , Seguridad , Vacunación/veterinaria , Ensayo de Placa Viral/veterinaria , Vacunas Virales/administración & dosificación , Fiebre del Nilo Occidental/prevención & controlRESUMEN
The enzootic or endemic strains of Venezuelan equine encephalomyelitis (VEE) virus (ID, IE, IF, and II-VI) are considered avirulent. In 1993 and 1996, outbreaks of encephalitis occurred in the horse populations in the Chiapas and Oaxaca provinces of Mexico, respectively. In both instances, enzootic VEE virus subserotype IE was isolated from brain tissues of dead horses. The present study investigated the pathogenicity of the Chiapas viral isolate (NVSL VEE IE 93-42124) in ponies. Three ponies were inoculated intradermally with 4, 5, and 6 logs, respectively, of the NVSL VEE IE 93-42124 viral isolate. All ponies showed fluctuations in body temperature, encephalitis, and other signs of infection with VEE virus. Virus was isolated only from the blood of ponies from day 1 to day 3 postinfection. Microscopic examination of hematoxylin and eosin-stained tissue sections showed mild to moderate nonsuppurative encephalitis, perivascular cuffing by mononuclear cells, gliosis, and meningoencephalitis. Antibody (IgM) to VEE virus IE was unable to differentiate between various subserotypes of VEE I viruses (serotypes IAB, IC, ID, and IF). Virus neutralizing antibody titers to heterologous VEE I viruses were 10-100-fold less than those for NVSL VEE IE 93-42124 virus and Mena II, a human isolate of VEE IE virus. The study confirmed that NVSL VEE IE 93-42124 virus, which was isolated from a brain of a horse during an outbreak of VEE in Chiapas, Mexico, was pathogenic for ponies.
Asunto(s)
Virus de la Encefalitis Equina Venezolana/patogenicidad , Encefalomielitis Equina Venezolana/veterinaria , Enfermedades de los Caballos/virología , Animales , Anticuerpos Antivirales/sangre , Encéfalo/patología , Encéfalo/virología , Virus de la Encefalitis Equina Venezolana/clasificación , Virus de la Encefalitis Equina Venezolana/inmunología , Encefalomielitis Equina Venezolana/patología , Encefalomielitis Equina Venezolana/fisiopatología , Encefalomielitis Equina Venezolana/virología , Ensayo de Inmunoadsorción Enzimática/veterinaria , Enfermedades de los Caballos/patología , Enfermedades de los Caballos/fisiopatología , Caballos , Inmunoglobulina M/sangre , Ratones , Pruebas de Neutralización/veterinaria , Serotipificación/veterinariaRESUMEN
Bovine sera from northeastern states (Connecticut, Delaware, Maine, Maryland, Massachusetts, New York, Pennsylvania, Vermont, and West Virginia), north central states (Indiana, Illinois, Iowa, Kentucky, Michigan, Minnesota, North Dakota, Ohio, South Dakota, and Wisconsin), Virginia, Alaska, and Hawaii were examined for the presence of neutralizing antibodies to Cache Valley (CV), Lokern (LK), Main Drain (MD), Northway (NW), and Tensaw (TS) viruses. Microneutralization tests were performed using Vero cells. Ninety percent inhibition of the virus at a 1:10 serum dilution was considered positive for the presence of specific antibody. Sera having antibody to more than one virus were titrated from 1:10 to 1:640. The results indicated that 4-28% of the cattle per region had specific antibodies to CV virus. Neutralizing antibodies to NW, LK, and TS viruses were also detected, indicating possible exposure to these Bunyamwera serogroup viruses along with CV virus. Antibody titers measured against NW virus were very similar to those against CV virus. Antibodies to MD virus were present in low levels in bovine sera from Illinois, Maryland, and Ohio. Cattle from Alaska had only antibodies to NW virus. Antibodies to Bunyamwera serogroup viruses were not observed in sera from Hawaii.