RESUMEN
[This corrects the article DOI: 10.1371/journal.pone.0150459.].
RESUMEN
Nitro-arachidonic acid (NO2-AA) is a cell signaling nitroalkene that exerts anti-inflammatory activities during macrophage activation. While angiotensin II (ANG II) produces an increase in reactive oxygen species (ROS) production and mitochondrial dysfunction in renal tubular cells, little is known regarding the potential protective effects of NO2-AA in ANG II-mediated kidney injury. As such, this study examines the impact of NO2-AA on ANG II-induced mitochondrial dysfunction in an immortalized renal proximal tubule cell line (HK-2 cells). Treatment of HK-2 cells with ANG II increases the production of superoxide (O2â-), nitric oxide (âNO), inducible nitric oxide synthase (NOS2) expression, peroxynitrite (ONOO-) and mitochondrial dysfunction. Using high-resolution respirometry, it was observed that the presence of NO2-AA prevented ANG II-mediated mitochondrial dysfunction. Attempting to address mechanism, we treated isolated rat kidney mitochondria with ONOO-, a key mediator of ANG II-induced mitochondrial damage, in the presence or absence of NO2-AA. Whereas the activity of succinate dehydrogenase (SDH) and ATP synthase (ATPase) were diminished upon exposure to ONOO-, they were restored by pre-incubating the mitochondria with NO2-AA. Moreover, NO2-AA prevents oxidation and nitration of mitochondrial proteins. Combined, these data demonstrate that ANG II-mediated oxidative damage and mitochondrial dysfunction is abrogated by NO2-AA, identifying this compound as a promising pharmacological tool to prevent ANG II-induced renal disease.
Asunto(s)
Angiotensina II/farmacología , Ácido Araquidónico/farmacología , Túbulos Renales Proximales/metabolismo , Mitocondrias/metabolismo , Adenosina Trifosfatasas/metabolismo , Línea Celular , Humanos , Túbulos Renales Proximales/efectos de los fármacos , Mitocondrias/efectos de los fármacos , Modelos Biológicos , Óxido Nítrico Sintasa/metabolismo , Oxidación-Reducción , Ácido Peroxinitroso/metabolismo , Succinato Deshidrogenasa/metabolismo , Superóxidos/metabolismoRESUMEN
Macrophage-derived nitric oxide ((â¢)NO) participates in cytotoxic mechanisms against diverse microorganisms and tumor cells. These effects can be mediated by (â¢)NO itself or (â¢)NO-derived species such as peroxynitrite formed by its diffusion-controlled reaction with NADPH oxidase-derived superoxide radical anion (O(2)(â¢-)). In vivo, the facile extracellular diffusion of (â¢)NO as well as different competing consumption routes limit its bioavailability for the reaction with O(2)(â¢-) and, hence, peroxynitrite formation. In this work, we evaluated the extent by which (â¢)NO diffusion to red blood cells (RBC) can compete with activated macrophages-derived O(2)(â¢-) and affect peroxynitrite formation yields. Macrophage-dependent peroxynitrite production was determined by boron-based probes that react directly with peroxynitrite, namely, coumarin-7-boronic acid (CBA) and fluorescein-boronate (Fl-B). The influence of (â¢)NO diffusion to RBC on peroxynitrite formation was experimentally analyzed in co-incubations of (â¢)NO and O(2)(â¢-)-forming macrophages with erythrocytes. Additionally, we evaluated the permeation of (â¢)NO to RBC by measuring the intracellular oxidation of oxyhemoglobin to methemoglobin. Our results indicate that diluted RBC suspensions dose-dependently inhibit peroxynitrite formation, outcompeting the O(2)(â¢-) reaction. Computer-assisted kinetic studies evaluating peroxynitrite formation by its precursor radicals in the presence of RBC are in accordance with experimental results. Moreover, the presence of erythrocytes in the proximity of (â¢)NO and O(2)(â¢-)-forming macrophages prevented intracellular Fl-B oxidation pre-loaded in L1210 cells co-cultured with activated macrophages. On the other hand, Fl-B-coated latex beads incorporated in the macrophage phagocytic vacuole indicated that intraphagosomal probe oxidation by peroxynitrite was not affected by nearby RBC. Our data support that in the proximity of a blood vessel, (â¢)NO consumption by RBC will limit the extracellular formation (and subsequent cytotoxic effects) of peroxynitrite by activated macrophages, while the intraphagosomal yield of peroxynitrite will remain unaffected.
Asunto(s)
Óxido Nítrico/metabolismo , Oxidación-Reducción , Ácido Peroxinitroso/metabolismo , Superóxidos/metabolismo , Animales , Difusión , Eritrocitos/metabolismo , Cinética , Macrófagos/metabolismo , Ratones , Ácido Peroxinitroso/biosíntesis , Fagosomas/metabolismoRESUMEN
Trypanosoma cruzi, the causative agent of Chagas disease, contains exclusively iron-dependent superoxide dismutases (Fe-SODs) located in different subcellular compartments. Peroxynitrite, a key cytotoxic and oxidizing effector biomolecule, reacted with T. cruzi mitochondrial (Fe-SODA) and cytosolic (Fe-SODB) SODs with second order rate constants of 4.6 ± 0.2 × 10(4) M(-1) s(-1) and 4.3 ± 0.4 × 10(4) M(-1) s(-1) at pH 7.4 and 37 °C, respectively. Both isoforms are dose-dependently nitrated and inactivated by peroxynitrite. Susceptibility of T. cruzi Fe-SODA toward peroxynitrite was similar to that reported previously for Escherichia coli Mn- and Fe-SODs and mammalian Mn-SOD, whereas Fe-SODB was exceptionally resistant to oxidant-mediated inactivation. We report mass spectrometry analysis indicating that peroxynitrite-mediated inactivation of T. cruzi Fe-SODs is due to the site-specific nitration of the critical and universally conserved Tyr(35). Searching for structural differences, the crystal structure of Fe-SODA was solved at 2.2 Å resolution. Structural analysis comparing both Fe-SOD isoforms reveals differences in key cysteines and tryptophan residues. Thiol alkylation of Fe-SODB cysteines made the enzyme more susceptible to peroxynitrite. In particular, Cys(83) mutation (C83S, absent in Fe-SODA) increased the Fe-SODB sensitivity toward peroxynitrite. Molecular dynamics, electron paramagnetic resonance, and immunospin trapping analysis revealed that Cys(83) present in Fe-SODB acts as an electron donor that repairs Tyr(35) radical via intramolecular electron transfer, preventing peroxynitrite-dependent nitration and consequent inactivation of Fe-SODB. Parasites exposed to exogenous or endogenous sources of peroxynitrite resulted in nitration and inactivation of Fe-SODA but not Fe-SODB, suggesting that these enzymes play distinctive biological roles during parasite infection of mammalian cells.
Asunto(s)
Proteínas Protozoarias/metabolismo , Superóxido Dismutasa/metabolismo , Trypanosoma cruzi/enzimología , Animales , Sitios de Unión/genética , Western Blotting , Dominio Catalítico , Enfermedad de Chagas/parasitología , Cristalografía por Rayos X , Cisteína/química , Cisteína/genética , Cisteína/metabolismo , Espectroscopía de Resonancia por Spin del Electrón , Transporte de Electrón , Activación Enzimática/efectos de los fármacos , Interacciones Huésped-Parásitos , Isoenzimas/química , Isoenzimas/genética , Isoenzimas/metabolismo , Cinética , Modelos Moleculares , Simulación de Dinámica Molecular , Mutagénesis Sitio-Dirigida , Nitratos/metabolismo , Ácido Peroxinitroso/química , Ácido Peroxinitroso/metabolismo , Ácido Peroxinitroso/farmacología , Unión Proteica , Estructura Secundaria de Proteína , Proteínas Protozoarias/química , Proteínas Protozoarias/genética , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/química , Superóxido Dismutasa/genética , Trypanosoma cruzi/genética , Trypanosoma cruzi/fisiología , Tirosina/química , Tirosina/genética , Tirosina/metabolismoRESUMEN
Excess production of superoxide (O2(-)) and nitric oxide (NO) in blood vessel walls may occur early in atherogenesis leading to the formation of peroxynitrite, a strong oxidant and nitrating agent. This study was designed to determine the effect of diphenyl diselenide (PhSe)2, a synthetic organoselenium compound, in comparison with ebselen, on peroxynitrite-mediated endothelial damage. Experimental results showed that pre-incubation of BAEC (24 h) with low concentrations of (PhSe)2 (0.5 and 1 µM) protected the cells from peroxynitrite-dependent apoptosis and protein tyrosine nitration. The intracellular levels of GSH were almost completely depleted by peroxynitrite and, although the compounds did not restore its normal levels, (PhSe)2 per se significantly increased GSH in a concentration-dependent manner. Moreover, (PhSe)2, which was about two times more active as a GPx mimic than ebselen, induced a significantly higher increase in both cellular GPx expression and activity. Taking into account the kinetics of the reaction between peroxynitrite and (PhSe)2, our data indicate that (PhSe)2 protects BAEC against peroxynitrite-mediated cell damage not by a direct reaction, but rather by increasing cellular GPx expression as a consequence of enhanced nuclear translocation of Nrf-2, which together with the increase in intracellular GSH, may work catalytically to reduce peroxynitrite to nitrite.
Asunto(s)
Apoptosis/efectos de los fármacos , Azoles/farmacología , Derivados del Benceno/farmacología , Células Endoteliales/efectos de los fármacos , Compuestos de Organoselenio/farmacología , Ácido Peroxinitroso/toxicidad , Sustancias Protectoras/farmacología , Análisis de Varianza , Animales , Aorta/citología , Aorta/efectos de los fármacos , Aorta/metabolismo , Bovinos , Supervivencia Celular/efectos de los fármacos , Células Endoteliales/citología , Células Endoteliales/metabolismo , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Isoindoles , Factor 2 Relacionado con NF-E2RESUMEN
SIGNIFICANCE: Chagas disease (CD) affects several million people in Latin America and is spreading beyond its classical boundaries due to the migration of infected host and insect vectors, HIV co-infection, and blood transfusion. The current therapy is not adequate for treatment of the chronic phase of CD, and new drugs are warranted. RECENT ADVANCES: Trypanosoma cruzi is equipped with a specialized and complex network of antioxidant enzymes that are located at different subcellular compartments which defend the parasite against host oxidative assaults. Recently, strong evidence has emerged which indicates that enzyme components of the T. cruzi antioxidant network (cytosolic and mitochondrial peroxiredoxins and trypanothione synthetase) in naturally occurring strains act as a virulence factor for CD. This precept is recapitulated with the observed increased resistance of T. cruzi peroxirredoxins overexpressers to in vivo or in vitro nitroxidative stress conditions. In addition, the modulation of mitochondrial superoxide radical levels by iron superoxide dismutase (FeSODA) influences parasite programmed cell death, underscoring the role of this enzyme in parasite survival. CRITICAL ISSUES: The unraveling of the biological significance of FeSODs in T. cruzi programmed cell death in the context of chronic infection in CD is still under examination. FUTURE DIRECTIONS: The role of the antioxidant enzymes in the pathogenesis of CD, including parasite virulence and persistence, and their feasibility as pharmacological targets justifies further investigation.
Asunto(s)
Enfermedad de Chagas/parasitología , Oxidorreductasas/fisiología , Proteínas Protozoarias/fisiología , Trypanosoma cruzi/fisiología , Factores de Virulencia/fisiología , Animales , Apoptosis , Enfermedad de Chagas/patología , Interacciones Huésped-Parásitos , Humanos , Óxido Nítrico/metabolismo , Estrés Oxidativo , Especies de Nitrógeno Reactivo/metabolismo , Trypanosoma cruzi/patogenicidadRESUMEN
Macrophage-derived radicals generated by the NADPH oxidase complex and inducible nitric-oxide synthase (iNOS) participate in cytotoxic mechanisms against microorganisms. Nitric oxide ((â¢)NO) plays a central role in the control of acute infection by Trypanosoma cruzi, the causative agent of Chagas disease, and we have proposed that much of its action relies on macrophage-derived peroxynitrite (ONOO(-) + ONOOH) formation, a strong oxidant arising from the reaction of (â¢)NO with superoxide radical (O(2)(-)). Herein, we have shown that internalization of T. cruzi trypomastigotes by macrophages triggers the assembly of the NADPH oxidase complex to yield O(2)(-) during a 60-90-min period. This does not interfere with IFN-γ-dependent iNOS induction and a sustained (â¢)NO production (â¼24 h). The major mechanism for infection control via reactive species formation occurred when (â¢)NO and O(2)() were produced simultaneously, generating intraphagosomal peroxynitrite levels compatible with microbial killing. Moreover, biochemical and ultrastructural analysis confirmed cellular oxidative damage and morphological disruption in internalized parasites. Overexpression of cytosolic tryparedoxin peroxidase in T. cruzi neutralized macrophage-derived peroxynitrite-dependent cytotoxicity to parasites and favored the infection in an animal model. Collectively, the data provide, for the first time, direct support for the action of peroxynitrite as an intraphagosomal cytotoxin against pathogens and the premise that microbial peroxiredoxins facilitate infectivity via decomposition of macrophage-derived peroxynitrite.
Asunto(s)
Citotoxinas/metabolismo , Macrófagos/enzimología , Óxido Nítrico Sintasa de Tipo II/metabolismo , Ácido Peroxinitroso/metabolismo , Proteínas Protozoarias/biosíntesis , Tiorredoxinas/biosíntesis , Trypanosoma cruzi/enzimología , Animales , Línea Celular , Enfermedad de Chagas/enzimología , Macrófagos/parasitología , Macrófagos/ultraestructura , Ratones , Óxido Nítrico/metabolismo , Oxidación-Reducción , Superóxidos/metabolismo , Trypanosoma cruzi/ultraestructuraRESUMEN
Activation of professional phagocytes with the concomitant generation of oxidant species is a medullar innate immune process for the control of acute Trypanosoma cruzi infection. Recent data reinforce the hypothesis that parasites more prepared to deal with the host-oxidative assault are more efficient for the establishment of Chagas disease. For instance, parasites overexpressing peroxiredoxins are more resistant to macrophage-derived peroxynitrite, a key cytotoxic oxidant produced in the phagosome towards the internalized parasite. Differentiation to the infective metacyclic trypomastigote is accompanied by an increased expression of antioxidant enzymes. Moreover, augmented antioxidant enzyme expression and activities correlate with higher parasite virulence in experimental infections. The potency of the parasite antioxidant armamentarium influences the final fate of the Trypanosoma cruzi journey to macrophage invasion at the onset of infection.
Asunto(s)
Enfermedad de Chagas/parasitología , Macrófagos/inmunología , Macrófagos/parasitología , Oxidantes/inmunología , Estrés Oxidativo , Estrés Fisiológico , Trypanosoma cruzi/inmunología , Trypanosoma cruzi/fisiología , Animales , Interacciones Huésped-Parásitos , Humanos , Oxidantes/metabolismoRESUMEN
Tobacco smoke is known to cause nitric oxide ((*)NO) inactivation and endothelial dysfunction. In this work we evaluated the interplay between (.)NO and superoxide (O(2)(*-)) radicals and the consequent impact on (*)NO bioavailability and nitroxidative stress in bovine aortic endothelial cells exposed to cigarette smoke extract (CSE) and in smokers. Bovine aortic endothelial cells in the presence of CSE triggered O(2)(*-) production as indicated by spin-trapping electron paramagnetic resonance experiments. O(2)(*-) was produced both extracellulary (3.4 vs. 1.0 nmol.h(-1)*mg(-1); CSE vs. control; cytochrome c(3+) reduction assay) and intracellularly (40% inhibition of cytosolic aconitase). CSE also led to the production of peroxynitrite as evaluated by dihydrorhodamine oxidation and protein tyrosine nitration on cells. O(2)(*-) and peroxynitrite formation were decreased by ascorbate and alpha-tocopherol. Additionally, CSE led to the oxidation of endothelial nitric oxide synthase increasing the monomeric inactive form of endothelial nitric oxide synthase. Smokers and age-matched healthy volunteers were supplemented orally with 500 mg ascorbate plus 400 IU all-rac-alpha-tocopherol every 12 h for 165 days. Smokers had endothelial dysfunction compared with control subjects (95% confidence interval: 2.5, 8.3 vs. 10.6, 14.2; P < 0.05) as assessed by flow-mediated dilation of the brachial artery, and plasma levels of protein 3-nitrotyrosine were 1.4-fold higher. The loss of flow-mediated dilation in smokers reverted after a long-term antioxidant supplementation (95% confidence interval: 13.9, 19.9; P < 0.05), reaching values comparable with the control population. Our data indicate that elements on tobacco smoke, most likely through redox cycling, divert (*)NO toward peroxynitrite by inducing O(2)(*-) production in vascular endothelial cells both in vitro and in vivo.
Asunto(s)
Antioxidantes/administración & dosificación , Células Endoteliales/metabolismo , Óxido Nítrico/metabolismo , Ácido Peroxinitroso/metabolismo , Fumar/metabolismo , Superóxidos/metabolismo , Adulto , Animales , Antioxidantes/metabolismo , Antioxidantes/farmacocinética , Aorta/citología , Ácido Ascórbico/administración & dosificación , Ácido Ascórbico/sangre , Ácido Ascórbico/farmacocinética , Arteria Braquial/fisiología , Bovinos , Células Cultivadas , Células Endoteliales/citología , Femenino , Humanos , Masculino , Óxido Nítrico Sintasa de Tipo III/metabolismo , Estrés Oxidativo/efectos de los fármacos , Estrés Oxidativo/fisiología , Fumar/efectos adversos , Adulto Joven , alfa-Tocoferol/administración & dosificación , alfa-Tocoferol/sangre , alfa-Tocoferol/farmacocinéticaRESUMEN
Protein tyrosine oxidation mechanisms in hydrophobic biocompartments (i.e., biomembranes, lipoproteins) leading to nitrated, dimerized, and hydroxylated products are just starting to be appreciated. This chapter reports on the use of the hydrophobic tyrosine analog N-t-BOC-l-tyrosine tert-butyl ester (BTBE) incorporated to phosphatidyl choline liposomes to study peroxynitrite-dependent tyrosine oxidation processes in model biomembranes. The probe proved to be valuable in defining the role of biologically relevant variables in the oxidation process, including the action of hydrophilic and hydrophobic peroxynitrite and peroxynitrite-derived free radical scavengers, transition metal catalysts, carbon dioxide, molecular oxygen, pH, and fatty acid unsaturation degree. Moreover, detection of the BTBE phenoxyl radical and relative product distribution yields of 3-nitro-, 3,3'-di-, and 3-hydroxy-BTBE in the membrane fully accommodate with a free radical mechanism of tyrosine oxidation, with physical chemical and biochemical determinants that in several respects differ of those participating in aqueous environments. The methods presented herein can be extended to explore the reaction mechanisms of tyrosine oxidation by other biologically relevant oxidants and in other hydrophobic biocompartments.
Asunto(s)
Liposomas , Técnicas de Sonda Molecular , Sondas Moleculares , Nitratos/metabolismo , Ácido Peroxinitroso/química , Proteínas/química , Tirosina/análogos & derivados , Tirosina/química , Dimerización , Espectroscopía de Resonancia por Spin del Electrón/métodos , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Hidroxilación , Modelos Biológicos , Ácido Peroxinitroso/metabolismo , Proteínas/metabolismo , Detección de Spin/métodos , Tirosina/metabolismoRESUMEN
Protein 3-nitrotyrosine is a posttranslational modification found in many pathological conditions from acute to chronic diseases. Could 3-nitrotyrosine formation participate on the basis of these diseases or is it just a marker connected with the associated nitroxidative stress? In vitro and in vivo data, including proteomic research, show that protein tyrosine nitration is a selective process where only a small amount of proteins is found nitrated and one or a few tyrosine residues are modified in each. Accumulating data suggest a strong link between protein 3-nitrotyrosine and the mechanism involved in disease development. In this review, we analyze the factors determining protein 3-nitrotyrosine formation, the functional and biological outcome associated with protein tyrosine nitration, and the fate of the nitrated proteins.
Asunto(s)
Biomarcadores/metabolismo , Nitratos/metabolismo , Proteínas/metabolismo , Tirosina/metabolismo , Secuencia de Aminoácidos , Datos de Secuencia Molecular , Proteínas/químicaRESUMEN
There is increasing evidence that Trypanosoma cruzi antioxidant enzymes play a key immune evasion role by protecting the parasite against macrophage-derived reactive oxygen and nitrogen species. Using T. cruzi transformed to overexpress the peroxiredoxins TcCPX (T. cruzi cytosolic tryparedoxin peroxidase) and TcMPX (T. cruzi mitochondrial tryparedoxin peroxidase), we found that both cell lines readily detoxify cytotoxic and diffusible reactive oxygen and nitrogen species generated in vitro or released by activated macrophages. Parasites transformed to overexpress TcAPX (T. cruzi ascorbate-dependent haemoperoxidase) were also more resistant to H2O2 challenge, but unlike TcMPX and TcCPX overexpressing lines, the TcAPX overexpressing parasites were not resistant to peroxynitrite. Whereas isolated tryparedoxin peroxidases react rapidly (k=7.2 x 10(5) M(-1) x s(-1)) and reduce peroxynitrite to nitrite, our results demonstrate that both TcMPX and TcCPX peroxiredoxins also efficiently decompose exogenous- and endogenously-generated peroxynitrite in intact cells. The degree of protection provided by TcCPX against peroxynitrite challenge results in higher parasite proliferation rates, and is demonstrated by inhibition of intracellular redox-sensitive fluorescence probe oxidation, protein 3-nitrotyrosine and protein-DMPO (5,5-dimethylpyrroline-N-oxide) adduct formation. Additionally, peroxynitrite-mediated over-oxidation of the peroxidatic cysteine residue of peroxiredoxins was greatly decreased in TcCPX overexpressing cells. The protective effects generated by TcCPX and TcMPX after oxidant challenge were lost by mutation of the peroxidatic cysteine residue in both enzymes. We also observed that there is less peroxynitrite-dependent 3-nitrotyrosine formation in infective metacyclic trypomastigotes than in non-infective epimastigotes. Together with recent reports of up-regulation of antioxidant enzymes during metacyclogenesis, our results identify components of the antioxidant enzyme network of T. cruzi as virulence factors of emerging importance.
Asunto(s)
Macrófagos/fisiología , Peroxirredoxinas/metabolismo , Ácido Peroxinitroso/farmacología , Tripanocidas/farmacología , Trypanosoma cruzi/enzimología , Animales , Enfermedad de Chagas/epidemiología , Enfermedad de Chagas/prevención & control , Humanos , Cinética , América Latina/epidemiología , Macrófagos/parasitología , Ratones , Mutagénesis Sitio-Dirigida , Peroxirredoxinas/genética , Proteínas Protozoarias/genética , Proteínas Protozoarias/metabolismo , Proteínas Recombinantes/metabolismo , Trypanosoma cruzi/efectos de los fármacos , VirulenciaRESUMEN
Carbonate radicals (CO3-) can be formed biologically by the reaction of OH with bicarbonate, the decomposition of the peroxynitrite-carbon dioxide adduct (ONOOCO2-), and enzymatic activities, i.e., peroxidase activity of CuZnSOD and xanthine oxidase turnover in the presence of bicarbonate. It has been reported that the spin-trap DMPO reacts with CO3(-) to yield transient species to yield finally the DMPO-OH spin adduct. In this study, the kinetics of reaction of CO3(-) with DMPO were studied by pulse radiolysis, yielding a second-order rate constant of 2.5 x 10(6) M(-1) s(-1). A Fenton system, composed of Fe(II)-DTPA plus H2O2, generated OH that was trapped by DMPO; the presence of 50-500 mM bicarbonate, expected to convert OH to CO3(-), markedly inhibited DMPO-OH formation. This was demonstrated to be due mainly to a fast reaction of CO3(-) with FeII-DTPA (k=6.1 x 10(8) M(-1) s(-1)), supported by kinetic analysis. Generation of CO3(-) by the Fenton system was further proved by analysis of tyrosine oxidation products: the presence of bicarbonate caused a dose-dependent inhibition of 3,4-dihydroxiphenylalanine with a concomitant increase of 3,3'-dityrosine yields, and the presence of DMPO inhibited tyrosine oxidation, in agreement with the rate constants with OH or CO3(-). Similarly, the formation of CO3(-) by CuZnSOD/H(2)O(2)/bicarbonate and peroxynitrite-carbon dioxide was supported by DMPO hydroxylation and kinetic competition data. Finally, the reaction of CO3(-) with DMPO to yield DMPO-OH was shown in peroxynitrite-forming macrophages. In conclusion, CO3(-) reacts quite rapidly with DMPO and may contribute to DMPO-OH yields in chemical and cellular systems; in turn, the extent of oxidation of other target molecules (such as tyrosine) by CO3(-) will be sensitive to the presence of DMPO.
Asunto(s)
Óxidos N-Cíclicos/química , Radicales Libres/química , Animales , Línea Celular , Espectroscopía de Resonancia por Spin del Electrón , Peróxido de Hidrógeno/química , Radical Hidroxilo/química , Hierro/química , Cinética , Macrófagos/metabolismo , Ratones , Radiólisis de Impulso , Marcadores de Spin , Superóxido Dismutasa/metabolismo , Tirosina/químicaRESUMEN
We have previously demonstrated that red blood cells (RBC) are an important sink of intravascularly generated peroxynitrite even in the presence of physiological concentrations of CO2 or other plasmatic biotargets. Once inside erythrocytes, peroxynitrite reacts fast with oxyhemoglobin (oxyHb; k2=2 x 10(4) M(-1) s(-1) at 37 degrees C and pH 7.4) and isomerizes to nitrate. Herein, we investigated whether, in spite of the fast diffusion and consumption of extracellularly added peroxynitrite by intraerythrocytic oxyHb, peroxynitrite-dependent radical processes could occur at the RBC membrane, focusing on tyrosine nitration. For this purpose, the hydrophobic tyrosine analogue N-t-BOC-L-tyrosine tert-butyl ester (BTBE) was successfully incorporated for the first time to a biological membrane, that is, RBC membrane, with incorporation yields approximately 1-3 x 10(7) molecules per RBC. The membrane integrity of BTBE-containing RBC was not significantly altered after BTBE incorporation as demonstrated by permeability studies. The probe was then used to study peroxynitrite-dependent reactions. The addition of peroxynitrite to BTBE-containing RBC suspensions resulted in BTBE nitration and dimerization to 3-nitro-BTBE and 3,3'-di-BTBE, respectively, indicative of peroxynitrite-derived radicals reactions in the membrane. Peroxynitrite addition to RBC also caused tyrosine nitration of membrane-associated proteins. The free radical nature of the process was also shown by the detection of protein-derived radicals by DMPO-immunospin trapping. While the presence of extracellular CO2 was potently inhibitory of intracellular oxyHb oxidation, membrane protein and BTBE nitration by peroxynitrite at Asunto(s)
Membrana Eritrocítica/química
, Ácido Peroxinitroso/química
, Tirosina/análogos & derivados
, Dióxido de Carbono/química
, Dimerización
, Radicales Libres
, Humanos
, Interacciones Hidrofóbicas e Hidrofílicas
, Nitrógeno/química
, Oxidación-Reducción
, Oxihemoglobinas/química
, Tirosina/química
RESUMEN
Hyperglycemic challenge to bovine aortic endothelial cells (BAECs) increases oxidant formation and cell damage that are abolished by MnSOD overexpression, implying mitochondrial superoxide (O(2)(.-)) as a central mediator. However, mitochondrial O(2)(.-) and its steady-state concentrations have not been measured directly yet. Therefore, we aimed to detect and quantify O(2)(.-) through different techniques, along with the oxidants derived from it. Mitochondrial aconitase, a sensitive target of O(2)(.-), was inactivated 60% in BAECs incubated in 30 mM glucose (hyperglycemic condition) with respect to cells incubated in 5 mM glucose (normoglycemic condition). Under hyperglycemic conditions, increased oxidation of the mitochondrially targeted hydroethidine derivative (MitoSOX) to hydroxyethidium, the product of the reaction with O(2)(.-), could be specifically detected. An 8.8-fold increase in mitochondrial O(2)(.-) steady-state concentration (to 250 pM) and formation rate (to 6 microM/s) was estimated. Superoxide formation increased the intracellular concentration of both hydrogen peroxide, measured as 3-amino-2,4,5-triazole-mediated inactivation of catalase, and nitric oxide-derived oxidants (i.e., peroxynitrite), evidenced by immunochemical detection of 3-nitrotyrosine. Oxidant formation was further evaluated by chloromethyl dichlorodihydrofluorescein (CM-H(2)DCF) oxidation. Exposure to hyperglycemic conditions triggered the oxidation of CM-H(2)DCF and was significantly reduced by pharmacological agents that lower the mitochondrial membrane potential, inhibit electron transport (i.e., myxothiazol), and scavenge mitochondrial oxidants (i.e., MitoQ). In BAECs devoid of mitochondria (rho(0) cells), hyperglycemic conditions did not increase CM-H(2)DCF oxidation. Mitochondrial O(2)(.-) formation in hyperglycemic conditions was associated with increased glucose metabolization in the Krebs cycle and hyperpolarization of the mitochondrial membrane.
Asunto(s)
Células Endoteliales/metabolismo , Glucosa/metabolismo , Peróxido de Hidrógeno/metabolismo , Mitocondrias/metabolismo , Estrés Oxidativo , Ácido Peroxinitroso/metabolismo , Superóxidos/metabolismo , Aconitato Hidratasa/metabolismo , Animales , Catalasa/antagonistas & inhibidores , Catalasa/metabolismo , Bovinos , Células Cultivadas , Ciclo del Ácido Cítrico , Células Endoteliales/efectos de los fármacos , Células Endoteliales/enzimología , Inhibidores Enzimáticos/farmacología , Fluoresceínas/química , Colorantes Fluorescentes/química , Hiperglucemia/metabolismo , Inmunohistoquímica , Potencial de la Membrana Mitocondrial , Microscopía Fluorescente , Mitocondrias/efectos de los fármacos , Mitocondrias/enzimología , Óxido Nítrico/metabolismo , Oxidación-Reducción , Fenantridinas/química , Factores de Tiempo , Triazoles/farmacología , Tirosina/análogos & derivados , Tirosina/metabolismoRESUMEN
Several pathologies of the cardiovascular system are associated with an augmented production of nitric oxide and/or superoxide-derived oxidants and/or alteration in the antioxidant detoxification pathways that lead to nitroxidative stress. One important consequence of these reactive intermediates at the biochemical level is the nitration of protein tyrosines, which is performed through either of two of the relevant nitration pathways that operate in vivo, namely peroxynitrite and heme peroxidase-dependent nitration. Proteins nitrated at tyrosine residues have been detected in several compartments of the cardiovascular system. In this review a selection of nitrated proteins in plasma (fibrinogen, plasmin, Apo-1), vessel wall (Apo-B, cyclooxygenase, prostaglandin synthase, Mn-superoxide dismutase) and myocardium (myofibrillar creatine kinase, alpha-actinin, sarcoplasmic reticulum Ca(2+) ATPase) are analyzed in the context of cardiovascular disease. Nitration of tyrosine can affect protein function, which could directly link nitroxidative stress to the molecular alterations found in disease. While some proteins are inactivated by nitration (e.g. Mn-SOD) others undergo a gain-of-function (e.g. fibrinogen) that can have an ample impact on the pathophysiology of the cardiovascular system. Nitrotyrosine is also emerging as a novel independent marker of cardiovascular disease. Pharmacological strategies directed towards inhibiting protein nitration will assist to shed light on the relevance of this post-translational modification to human cardiovascular pathology.
Asunto(s)
Enfermedades Cardiovasculares/metabolismo , Sistema Cardiovascular/metabolismo , Óxido Nítrico/metabolismo , Transducción de Señal/fisiología , Tirosina/metabolismo , Animales , Fibrinógeno/metabolismo , Humanos , Nitrosación , Superóxido Dismutasa/metabolismo , Tirosina/análogos & derivadosRESUMEN
Trypanosoma cruzi undergo PCD (programmed cell death) under appropriate stimuli, the mechanisms of which remain to be established. In the present study, we show that stimulation of PCD in T. cruzi epimastigotes by FHS (fresh human serum) results in rapid (<1 h) externalization of phosphatidylserine and depletion of the low molecular mass thiols dihydrotrypanothione and glutathione. Concomitantly, enhanced generation of oxidants was established by EPR and immuno-spin trapping of radicals using DMPO (5,5-dimethylpyrroline-N-oxide) and augmentation of the glucose flux through the pentose phosphate pathway. In the early period (<20 min), changes in mitochondrial membrane potential and inhibition of respiration, probably due to the impairment of ADP/ATP exchange with the cytosol, were observed, conditions that favour the generation of O2*-. Accelerated rates of mitochondrial O2*- production were detected by the inactivation of the redox-sensitive mitochondrial aconitase and by oxidation of a mitochondrial-targeted probe (MitoSOX). Importantly, parasites overexpressing mitochondrial FeSOD (iron superoxide dismutase) were more resistant to the PCD stimulus, unambiguously indicating the participation of mitochondrial O2*- in the signalling process. In summary, FHS-induced PCD in T. cruzi involves mitochondrial dysfunction that causes enhanced O(2)(*-) formation, which leads to cellular oxidative stress conditions that trigger the initiation of PCD cascades; moreover, overexpression of mitochondrial FeSOD, which is also observed during metacyclogenesis, resulted in cytoprotective effects.
Asunto(s)
Apoptosis , Citoprotección , Mitocondrias/metabolismo , Superóxido Dismutasa/metabolismo , Superóxidos/metabolismo , Trypanosoma cruzi/citología , Trypanosoma cruzi/metabolismo , Animales , Citocromos c/metabolismo , Regulación Enzimológica de la Expresión Génica , Glutatión/análogos & derivados , Glutatión/metabolismo , Inmunohistoquímica , NADH NADPH Oxidorreductasas/metabolismo , Estrés Oxidativo , Espermidina/análogos & derivados , Espermidina/metabolismo , Superóxido Dismutasa/genética , Trypanosoma cruzi/genéticaRESUMEN
Most of the mechanistic studies of tyrosine nitration have been performed in aqueous solution. However, many protein tyrosine residues shown to be nitrated in vitro and in vivo are associated to nonpolar compartments. In this work, we have used the stable hydrophobic tyrosine analogue N-t-BOC-L-tyrosine tert-butyl ester (BTBE) incorporated into phosphatidylcholine (PC) liposomes to study physicochemical and biochemical factors that control peroxynitrite-dependent tyrosine nitration in phospholipid bilayers. Peroxynitrite leads to maximum 3-nitro-BTBE yields (3%) at pH 7.4. In addition, small amounts of 3,3'-di-BTBE were formed at pH 7.4 (0.02%) which increased over alkaline pH; at pH 6, a hydroxylated derivative of BTBE was identified by HPLC-MS analysis. BTBE nitration yields were similar in dilauroyl- and dimyristoyl-PC and were also significant in the polyunsaturated fatty acid-containing egg PC. *OH and *NO2 scavengers inhibited BTBE nitration. In contrast to tyrosine in the aqueous phase, the presence of CO2 decreased BTBE nitration, indicating that CO3*- cannot permeate to the compartment where BTBE is located. On the other hand, micromolar concentrations of hemin and Mn-tccp strongly enhanced BTBE nitration. Electron spin resonance (ESR) detection of the BTBE phenoxyl radical and kinetic modeling of the pH profiles of BTBE nitration and dimerization were in full agreement with a free radical mechanism of oxidation initiated by ONOOH homolysis in the immediacy of or even inside the bilayer and with a diffusion coefficient of BTBE phenoxyl radical 100 times less than for the aqueous phase tyrosyl radical. BTBE was successfully applied as a hydrophobic probe to study nitration mechanisms and will serve to study factors controlling protein and lipid nitration in biomembranes and lipoproteins.
Asunto(s)
Membrana Dobles de Lípidos/química , Sondas Moleculares/química , Ácido Peroxinitroso/química , Tirosina/análogos & derivados , Tirosina/química , Dióxido de Carbono , Cromatografía Líquida de Alta Presión , Dimerización , Ácidos Grasos Insaturados/química , Interacciones Hidrofóbicas e Hidrofílicas , Liposomas/química , Membranas/química , Nitrógeno/química , Oxidación-Reducción , Fosfatidilcolinas/química , Análisis EspectralRESUMEN
We studied the capacity of macrophage-derived peroxynitrite to diffuse into and exert cytotoxicity against Trypanosoma cruzi, the causative agent of Chagas' disease. In two types of macrophage-T. cruzi co-cultures, one with a fixed separation distance between source and target cells, and another involving cell-to-cell interactions, peroxynitrite resulted in significant oxidation of intracellular dihydrorhodamine and inhibition of [(3)H]thymidine incorporation in T. cruzi, which were not observed by superoxide or nitric oxide alone. The effects were attenuated in the presence of bicarbonate, in agreement with the extracellular consumption of peroxynitrite by its fast reaction with CO(2). However, studies using different T. cruzi densities, which allow to modify average diffusion distances of exogenously added peroxynitrite to target cells, indicate that at distances <5 microm, the diffusion process outcompetes the reaction with CO(2) and that the levels of peroxynitrite formed by macrophages would be sufficient to cause toxicity to T. cruzi during cell-to-cell contact and/or inside the phagosome.
Asunto(s)
Comunicación Celular/fisiología , Macrófagos/metabolismo , Oxígeno/metabolismo , Ácido Peroxinitroso/biosíntesis , Ácido Peroxinitroso/farmacología , Trypanosoma cruzi/efectos de los fármacos , Trypanosoma cruzi/fisiología , Animales , Comunicación Celular/efectos de los fármacos , Línea Celular , Proliferación Celular/efectos de los fármacos , Respiración de la Célula/efectos de los fármacos , Respiración de la Célula/fisiología , Técnicas de Cocultivo/métodos , Difusión , Ratones , Oxidantes/farmacología , Oxidación-Reducción/efectos de los fármacos , Ácido Peroxinitroso/química , Especies Reactivas de Oxígeno/metabolismoRESUMEN
OBJECTIVE: Decreased diaphragmatic contractility and organ failure observed during sepsis is mediated by an overproduction of nitric oxide ((.)NO)-derived species, mitochondria being a major target of oxidative and nitrative stress. We tested the potential protective effects of (a) a novel synthetic antioxidant, the manganese(III) 5,10,15,20-tetrakis(N-ethylpyridinium-2-yl) porphyrin (MnTE-2-PyP(5+)) and (b) the inducible (.)NO synthase inhibitor aminoguanidine (AG) on a rat model of sepsis. SETTING: University research laboratories. SUBJECTS AND INTERVENTIONS: Sepsis was induced by cecal ligation and perforation in rats. MEASUREMENTS AND RESULTS: Systemic hemodynamics, pulmonary gas exchange, in vitro diaphragmatic function and mitochondrial respiration were evaluated. Moreover, plasma and mitochondrial oxidative and nitrative stress parameters were investigated. Sepsis determined diaphragmatic dysfunction and a significant decrease in mitochondrial coupling and respiration. Oxidative stress was evidenced by decreased plasma antioxidants and increased lipid oxidation. Tyrosine nitration was increased in the plasma and mitochondria of the septic animals. These alterations were ameliorated or prevented by either MnTE-2-PyP(5+) or AG. CONCLUSIONS: Our results demonstrate that overproduction of (.)NO and (.)NO-derived reactive species play a critical role in mitochondrial impairment and diaphragmatic function during sepsis. More importantly, AG but mainly the novel metalloporphyrin MnTE-2-PyP(5+) were able to ameliorate diaphragmatic and mitochondrial dysfunction and could contribute to preventing organ failure during severe sepsis.