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Arch Virol ; 162(7): 2083-2090, 2017 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-28352973

RESUMEN

Overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in Thailand, were screened by RT-PCR using primers Polero-CP-F and Polero-CP-R. A total of 160 samples (~66%) were infected by poleroviruses. Analysis of a 1.4 kb region covering the 3' RNA-dependent RNA polymerase (RdRp) gene, the intergenic non-coding region (iNCR), and the coat protein (CP), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (CABYV), luffa aphid-borne yellows virus (LABYV), melon aphid-borne yellows virus (MABYV) and suakwa aphid-borne yellows virus (SABYV) were associated with the yellowing symptoms in cucurbit crops. Further analyses indicated presence of putative recombinant viruses referred to as CABYV-R and SABYV-R. CABYV-R was derived from the recombination between MABYV and the common strain of CABYV (CABYV-C). SABYV-R was derived from the recombination of MABYV and SABYV.


Asunto(s)
Cucurbita/virología , Luteoviridae/genética , Enfermedades de las Plantas/virología , Malezas/virología , ARN Polimerasa Dependiente del ARN/genética , Productos Agrícolas/virología , Genoma Viral , Luteoviridae/clasificación , Luteoviridae/aislamiento & purificación , Sistemas de Lectura Abierta , Filogenia , Análisis de Secuencia de ADN , Tailandia
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