The Effects of Honeysuckle (Lonicera caerulea L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental Trichinellosis.

The aim of the study was to determine the effect of iridoid-anthocyanin extract from honeysuckle (Lonicera caerulea L.) (LC) berries on histopathological changes in the intestines and muscles during experimental trichinellosis in mice. The LC extract was administered to uninfected mice (LC group) and Trichinella-spiralis-infected mice (T+LC) orally at a dose of 2 g/kg bw, six times at 24 h intervals, from day 3 prior to infection to day 3 post-infection (dpi). Jejunum samples were collected on 5, 7, 14, and 21 dpi, and their histological assessment involved the villus height to crypt depth ratio (VH/CD), goblet cell (GC) number, and morphological changes. In the T. spiralis-infected muscles, the extent of inflammatory infiltration on the 14th and 21st dpi was assessed. LC in the infected mice restored the VH/CD ratio to control values on 14 dpi. A beneficial effect of the LC extract on the villus height was also observed 14 dpi in the LC and T+LC groups. No differences in the extent of inflammatory infiltration in the muscles between the T+LC and T groups were observed. In conclusion, the iridoid-anthocyanin extract from honeysuckle berry contributed to alleviating the symptoms of the intestinal phase of T. spiralis infection.

Weather parameters as a predictive tool potentially allowing for better monitoring of dairy cattle against gastrointestinal parasites hazard.

In animal production, yield is critically related to animal health status. To ensure high productivity, innovative control strategies for herd and parasites monitoring are required. Gastrointestinal parasites have a strong influence on changing feed intake or nutrient use, limiting animal productivity. Serological control has been proposed, given that parasite development is largely dependent on environmental temperature and humidity. However, breeders and field veterinarians lack readily accessible climate characteristics that provide information to determine whether and when herds require laboratory examination. To help reduce the testing costs incurred by farmers, we investigated whether selected meteorological data could serve as conclusive predictors to increase the precision of herd selection for serological monitoring. Our results indicate that the selection of herds by farmers for testing can be guided by regular checking of meteorological data, especially various temperature and humidity indicators. In general, ranges of 24-28 °C, as well as - 0.5 to 7.5 °C for the monthly maximum and minimum temperature, respectively, and relative humidity (68-79%) and vapour pressure (10-15 hPa) correspond to a high antiparasitic response of the herd, expressed as the optical density ratio. It is recommended to introduce coproscopic and/or serological tests if the observed weather pattern (covering the prepatent period of parasite development) ranges within the estimated values.

Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (Lonicera caerulea L.) in the Course of Experimental Trichinellosis in Mice.

Our experiment determined the immunotropic activity of a natural, iridoid-anthocyanin extract from honeysuckle berry (Lonicera caerulea L.) (LC). The extract was administered to mice infected with Trichinella spiralis, orally at a dose of 2 g/kg bw, six times at 24 h intervals (from day 3 prior to the infection to day 3 post-infection (dpi) with T. spiralis. At 5, 7, 14, and 21 dpi, samples of blood, spleen, and mesenteric lymph nodes (MLN) were collected, and isolated lymphocytes were analyzed by flow cytometry. The splenocyte proliferation was estimated with MTT testing, and the intensity of intestinal and muscle infection was also studied. LC stimulated the local immune system by inducing lymphocyte proliferation in the spleen 7 dpi and altered the percentage and absolute count of B (CD19+) and T (CD3+, CD8+) cells 7, 14, and 21 dpi in the peripheral blood. LC extract affected the dynamics of expulsion of adult Trichinella from the intestines and prolonged the intestinal phase of the infection but did not change the number of larvae in the muscles. These results suggest that Lonicera caerulea L. fruit extract modulates murine cellular immune response during intestinal phase of T. spiralis infection but shows no antiparasitic activity.

Cryptosporidium spp. in dogs and cats in Poland.

INTRODUCTION: Cryptosporidium spp. are common protozoan parasites of animals and humans. Due to their zoonotic potential it is important to know their species and prevalence in dogs and cats. OBJECTIVE: The aim of the study was to determine the occurrence and molecular characteristics of Cryptosporidium spp. in dogs and cats in Poland. MATERIAL AND METHODS: A total of 365 faecal samples (264 dogs and 101 cats) collected from animals living in Poland were analyzed using the Ziehl-Neelsen staining method and genus-specific PCR assay to amplify the Cryptosporidium 18S rRNA gene. RESULTS: Cryptosporidium were found in 11 out of the 365 examined stool samples (3%). PCR analysis identified Cryptosporidium in 9 out of 264 canine stool samples (3.4%) and 2 out of 101 feline specimens (2%). DNA sequencing confirmed the presence of C. canis and C. parvum in dogs and C. felis in cats. CONCLUSIONS: This is the first molecular characterization of Cryptosporidium spp. infection in dogs and cats in Poland.

Genotypes of Giardia duodenalis in Household Dogs and Cats from Poland.

BACKGROUND: Giardia duodenalis is a widespread protozoan parasite affecting humans and many species of animals, including dogs and cats. Due to its zoonotic potential, it is important to know the frequency of this parasite in companion animals. The aim of this study was to determine current epidemiological status of G. duodenalis in household dogs and cats. METHODS: In this study, 293 fecal samples from pet dogs and cats were collected from January 2017 to July 2019 and tested for G. duodenalis by PCR (using ß-giardin gene). The animals were divided into groups depending on their age, breed and fecal consistency. RESULTS: The examination allowed for detection of G. duodenalis in 6.0% of canine and 3.9% of feline fecal samples. The highest frequency was revealed in young (under one-year old) dogs. Sequencing confirmed the presence of assemblages C and D in dogs and A and F in cats. CONCLUSION: The study showed current frequency of G. duodenalis in dogs and cats and also revealed the occurrence of host-specific assemblages as well as zoonotic assemblage A.

Effects of iridoid-anthocyanin extract of Cornus mas L. on hematological parameters, population and proliferation of lymphocytes during experimental infection of mice with Trichinella spiralis.

The influence of iridoid-anthocyanin aqueous extract of cornelian cherry fruits (CM) on hematological parameters, lymphocyte subsets and proliferation during Trichinella spiralis infection in mice was investigated. CM (100 mg/kg) was administered orally to T. spiralis-infected mice six times within a period encompassing three days prior to the infection and three days after the infection (dai). CM increased the percentage of CD3+, CD4+ cells and CD4+/CD8+ ratio and decreased total count of CD8+ and CD19+ splenocytes (5th dai). An increase in total count of CD4+, CD3+, CD19+ splenocytes was observed (21st dai). CM elevated the percentage of CD4+ cells (7th dai) and CD4+/CD8+ ratio (21st dai) in MLN. CM increased (14th dai) and then reduced (21st dai) the percentage of CD8+ MLN lymphocytes and decreased total count of MLN CD8+ cells (21st dai) and B cells (14th dai). An activation of lymphocyte proliferation in spleen and simultaneous decrease in MLN on 5th dai was observed. An increase in red blood cells parameters (5th dai) and in leukocyte count (7th dai) was found. A rise in platelet count was noticed both on 5th and 7th dai. Moreover, the number of adult T. spiralis on 5th dai in mice receiving CM extract was lower than in the control mice. These results suggested that iridoid-anthocyanin aqueous extract of CM stimulated murine immune response during T. spiralis infection.

Zoonotic microsporidia in dogs and cats in Poland.

This study investigated the prevalence, genetic diversity, and zoonotic concerns of microsporidia in household dogs and cats in Poland. A total of 126 (82 dogs and 44 cats) fecal specimens were analyzed for the presence of specific DNA of Enterocytozoon bieneusi and Encephalitozoon spp. using a nested PCR protocol amplifying the internal transcribed spacer region of the rRNA gene. Microsporidia were found in 10 (7.9%) out of the 126 examined stool samples. Of the 82 dogs, 4 (4.9%) and 2 (2.4%) were positive for E. bieneusi (genotypes D and PtEbIX) and Encephalitozoon cuniculi genotype II, respectively. Of the 44 cats, 4 (9.1%) were positive for E. bieneusi (genotypes PtEbIX and eb52). Additionally, one cat (2.3%) was concurrently infected with E. bieneusi (PtEbIX) and E. cuniculi (genotype II). Considering that all detected microsporidia in dogs and cats have been previously associated with human microsporidiosis, companion animals may be a potential source of microsporidia infections in humans.

Molecular identification of Giardia duodenalis isolates from domestic dogs and cats in Wroclaw, Poland.

INTRODUCTION: Giardia duodenalis (G. intestinalis) is a common protozoan causing gastrointestinal disorders in many species of mammals. The genus of Giardia has high molecular diversity. Dogs and cats, in addition to their typical infection with assemblages C, D and F, may be a reservoir of zoonotic assemblages (A and B). OBJECTIVE: The aim of this study was a genetic characteristic of Giardia isolates of dogs and cats from the area of Wroclaw (Poland). MATERIALS AND METHOD: A total of 128 and 33 faecal samples from dogs and cats, respectively, were analyzed by routine coprological methods. The animals were diagnosed on the presence of G. duodenalis antigens in faeces soluble with the use of SNAP Giardia (IDEXX Laboratories) immunosorbent assay. 27 DNA isolates of Giardia were subjected to molecular identification (PCR-RFLP). RESULTS AND CONCLUSIONS: The prevalence of G. duodenalis was 21.1% (27/128) in dogs and 15.1% (5/33) in cats. In dogs, C assemblage was present in 18 (81%) positive stool samples, D assemblage in 2 (9%) samples, B assemblage present in one (4.5%), and mixed assemblages (C and D) occurred in one (4.5%) sample. F assemblage was found in 4 (80%) cats' positive stool samples and A assemblage occurred in one case (20%). Confirmation of the presence of A and B zoonotic assemblages suggests that infected pets can be a threat to human health. This study describes for the first time the presence of mixed infections within host-specific C and D assemblages in dogs in Poland.

Serological detection of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato and Ehrlichia canis antibodies and Dirofilaria immitis antigen in a countrywide survey in dogs in Poland.

Canine vector-borne diseases (CVBDs) have increasingly become a focus of attention in the past few years. Nevertheless, in many parts of Europe information on their occurrence is still scarce. In a large study in Poland 3,094 serum samples taken from dogs throughout all 16 Polish provinces were tested using a commercial kit for the detection of circulating antibodies against Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato and Ehrlichia canis and of Dirofilaria immitis antigen. A total of 12.31% (381/3,094; 95% confidence interval [CI]: 11.18-13.52%) and 3.75% (116/3,094; 95% CI: 3.11-4.48%) of the dogs were positive for A. phagocytophilum and B. burgdorferi s.l. antibodies, respectively. Furthermore, 0.26% (8/3,094; 95% CI: 0.11-0.51%) were positive for E. canis antibodies and 0.16% (5/3,094; 95% CI: 0.05-0.38%) for D. immitis antigen. The highest percentages of A. phagocytophilum-positive dogs were noted in Lesser Poland, Silesia and Lódz Provinces. For B. burgdorferi s.l., the highest prevalence was recorded in Lódz Province. Co-infections with A. phagocytophilum and B. burgdorferi s.l. were recorded in 1.71% of all examined dogs (53/3,094; 95% CI: 1.29-2.23%). One dog even had a triple infection, testing positive for E. canis too. Both A. phagocytophilum and B. burgdorferi s.l. have previously been reported in Poland and were confirmed in the present study by positive samples from all 16 provinces. Concerning E. canis and D. immitis travel history or importation cannot be excluded as factors which may have determined the occurrence of these pathogens in the relevant animals. Practitioners in Poland should be aware of the above mentioned CVBDs and of prophylactic measures to protect dogs and their owners.

Occurrence of tapeworms of the family Anoplocephalidae in herds of dairy cattle in Lesser Poland and in Lower Silesia, Poland.

This study was performed to determine the tapeworm infection of grazing cattle in 11 dairy herds in Lower Silesia and Lesser Poland (Galicia). Rectal faecal samples were examined microscopically for tapeworm eggs by Willis-Shlaaf's flotation, Telemann's sedimentation (for fatty stools) and decantation method. Out of 182 cattle, 10 (5.5%) were found to be infected with tapeworms. The prevalence in 5 of 11 examined herds varied from 15.5 to 30.5% and was highest for animal being in the first lactation. Tapeworm eggs were detected in animals being in the first and second lactation (about three- to four-years-old cows). All the qualitative methods were effective in the diagnosis of tapeworm infection in cattle.

[The apoptosis in the course of experimental infection with T. pseudospiralis in mice]. / Proces apoptozy w przebiegu doswiadczalnego zarazenia T. pseudospiralis u myszy.

The apoptosis in the course of experimental infection with T. pseudospiralis in mice. The level of apoptic cells in the lamina propria of the mucosa of the jejunum and in the masseter muscle in the mice infected with 200 larvae T. pseudospiralis was observed. Cryostat preparations made from jejunum and muscle samples on 7, 14, 21, 28, 60, 180 and 360 day post infection (dpi), were examined employing the TUNNEL method with the use of "In situ Cell Death Detection KIT Fluorescein" of Roche. The number of apoptic cells in the lamina propria of jejunum started to increase as early as on 7 dpi. The highest number of the apoptic cells, on an average - 56,4 in the crypta-villus unit, was recorded on 28 dpi, whereas the return to the standard level took place only 360 dpi. The level of the apoptic cells in the masseter muscle was the highest on 28 and 60 dpi, which constituted some 30% of all the cells of infiltration.