RESUMEN
Sugarcane root systems are poorly studied and understood due to the perennial nature, tall stature, and the long cropping cycle. Whilst some field studies gave insights into sugarcane root traits, there is no detailed description of root and root system traits available. The objectives of our work were to establish a baseline of sugarcane root trait values that will serve for future studies, and to characterize the degree of root system resilience when restricting tiller number. We first conducted an initial screening for root trait diversity on a collection of twenty cultivars representative of sugarcane breeding from 1930 to now. Then we investigated the effect of reduced tillering, via manual de-tillering, on the plant root and root system traits of five varieties grown under optimal conditions in a glasshouse for 1700°Cd. In addition to establishing baseline means and variation for sugarcane root trait values that could serve as a reference for crop models, we demonstrated that the sugarcane root mass fraction was extremely resilient to drastic reduction in tiller number. Restricted plants were effectively maintaining their root system configuration (opening angle) by dramatically increasing the number of nodal roots produced per tiller as well as maximizing total root length by increasing the specific root length. Using this knowledge of sugarcane root traits in combination with the specific agronomic constraints for sugarcane will now underpin the development of a root system ideotype for sugarcane to enable targeted root trait selection for improving crop productivity.
RESUMEN
The recent development of genetically modified sugarcane, with the aim of commercial production, requires an understanding of the potential risks of increased weediness of sugarcane as a result of spread and persistence of volunteer sugarcane. As sugarcane is propagated vegetatively from pieces of stalk and the seed plays no part in the production cycle, the fate of seed in the environment is yet to be studied. In this study, sugarcane seed samples, collected in fields over a 2-year period, were used to determine the overall level of sugarcane fertility, seed dormancy, and longevity of seed under field conditions. A survey of the soil seed bank in and around sugarcane fields was used to quantify the presence of sugarcane seeds and to identify and quantify the weeds that would compete with sugarcane seedlings. We demonstrated that under field conditions, sugarcane has low fertility and produces non-dormant seed. The viability of the seeds decayed rapidly (half-life between 1.5 and 2.1 months). This means that, in Australia, sugarcane seeds die before they encounter climatic conditions that could allow them to germinate and establish. Finally, the soil seed bank analysis revealed that there were very few sugarcane seeds relative to the large number of weed seeds that exert a large competitive effect. In conclusion, low fertility, short persistence, and poor ability to compete limit the capacity of sugarcane seed spread and persistence in the environment.
RESUMEN
Hypocotyl growth is a key characteristic for plant emergence, influenced by environmental conditions, particularly temperature, and varying among genotypes. Cellular changes in Medicago truncatula hypocotyl were characterized to study the impact of the environment on heterotrophic growth and analyze differences between genotypes. The number and length of epidermal cells, ploidy levels, and sugar contents were measured in hypocotyls grown in the dark at 20 °C and 10 °C using two genotypes with contrasting maximum hypocotyl length. Hypocotyl elongation in the dark was due to cell elongation and not to an increase in cell number. A marked increase in cell ploidy level was observed just after germination and until mid elongation of the hypocotyl under all treatments. Larger ploidy levels were also observed in the genotype with the shorter hypocotyl and in cold conditions, but they were associated with larger cells. The increase in ploidy level and in cell volume was concomitant with a marked increase in glucose and fructose contents in the hypocotyl. Finally, differences in hypocotyl length were mainly due to different number of epidermal cells in the seed embryo, shown as a key characteristic of genotypic differences, whereas temperature during hypocotyl growth affected cell volume.
