Efficiency of plant-based acaricide gels compared to fluvalinate-impregnated strips for control of Varroa destructor in honey bee colonies.

The Varroa mite is one of the most important pests of honey bee colonies. This study compares the efficiency of thymol-based acaricides with the tau-fluvalinate-based commercial treatment to control this mite species. Two experiments were conducted: (1) in the Fall, thymol-based gel was compared with a widely used acaricide, fluvalinate-impregnated strips (Apistan®), and (2) in the Spring, gels containing thymol-only, thymol + menthol + eucalyptus essential oil, and thymol + thyme essential oil were compared with fluvalinate-impregnated strips. In the first experiment, both treatments reduced Varroa mite infestation levels significantly after 70 days of their application compared to the control treatment. The results of the second experiment showed that thymol + thyme essential oil gel, thymol + menthol + eucalyptus essential oil gel, and Apistan strips had always higher efficiency on decreasing mite infestation levels of the selected colonies compared to the control treatment and thymol-only gel during their 7-week application. The results of this study demonstrate that plant-based acaricides for controlling Varroa mites in honey bee colonies have similar efficiency compared to the chemical control methods. Therefore, it is better to use these selected acaricides to reduce mite resistance to chemical control treatments in honey bee colonies.

Soil Fertilization With Medicinal Plant Processing Wastes Suppresses Tuta absoluta (Lepidoptera: Gelechiidae) and Aphis gossypii (Hemiptera: Aphididae) Populations.

Organic soil amendments can influence insect pest populations and the damage to plants they cause. In this study, the effects of medicinal plant processing wastes (MPPWs) applied as organic fertilizers on the host preference and performance of Tuta absoluta and Aphis gossypii were investigated on tomato and cucumber plants, respectively. Processing wastes of cumin, rosemary, thyme, artichoke, chamomile, fenugreek, and nettle were applied in four levels of 0, 20, 40, and 80 g dry matter/1kg culture media in pot experiments. Results showed the application of MPPWs, especially 80 g of nettle, reduced the number of T. absoluta eggs (from 0.8 to 0.4 egg/leaf) and their hatching percentage (from 90 to 76%). The highest and lowest number of aphids were observed in control (36 aphids/plant) and treated cucumbers with 80 g of cumin (18 aphids/plant). Also, the lowest intrinsic rate of increase (0.08 d-1) and net reproductive rate (20 offspring) of T. absoluta were observed in tomatoes fertilized with nettle. The highest and lowest net reproductive rate of A. gossypii were obtained on control and treated plants with 80 g of nettle, respectively. Results of damage assessment showed that the percentage of dry weight loss in the aphid-infested plants was reduced by the use of MPPWs, so that lowest weight loss was observed in the treatment with 80 g of nettle. In conclusion, soil amendment using MPPWs could result in lower pest populations and may improve plant tolerance to insect pest stress, thus these by-products could be considered a valuable tool in pest management.

Simultaneous Occurrence of Diapause and Cold Hardiness in Overwintering Eggs of the Apple Oystershell Scale, Lepidosaphes Malicola Borchsenius (Hem.: Diaspididae).

As the key pest of apple fruits, the oystershell scale, Lepidosaphes malicola Borchsenius (Hem.: Diaspididae), overwinters as diapausing eggs under the protective, waxy cover of females. In this research, the effects of diapause development, cold acclimation, and rapid cold hardening were studied on the cold hardiness of the eggs. The changes in some physiological components were also investigated. The results indicated cold exposure to be a prerequisite for the survival of the diapausing eggs of L. malicola. No eggs hatched without exposure to cold. In addition, a direct relationship was observed among cold hardiness, cold acclimation, and diapause of the eggs based on the results. The highest level of hatching (the highest cold hardiness) of the eggs (80%) occurred in the cold-acclimated eggs at the end of diapause (March). Rapid cold hardening also influenced the cold hardiness of the eggs with diapause development. At the end of diapause, the lowest (61%) and the highest (77%) rates of egg survival were observed when the eggs were exposed to 5 and -10°C for 24 h, respectively. Cold hardiness of the diapausing eggs of L. malicola was also accompanied by some physiological changes, i.e., a decrease in glycogen content and an increase in simple sugar, lipid, and protein contents. The lowest glycogen content (about 50 µg/g) and the highest amounts of total simple sugars (454 µg/g) of lipids (542 µg/g) and proteins (84 µg/g) were observed in the cold-acclimated eggs at the end of diapause.

Side effects of chlorantraniliprole, phosalone and spinosad on the egg parasitoid, Trichogramma brassicae.

The combined use of chemicals and biological control is not always a successful strategy owing to the potential side effects on biocontrol agents. Lethal and sublethal effects of three commonly used insecticides were assessed on adult and immature stages of the egg parasitoid Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae). Recommended field concentrations of chlorantraniliprole, phosalone and spinosad caused mortality on preimaginal stages by 24, 87, and 98%, respectively. Lethal effects on parasitoid adults exposed to the insecticide dry residues were estimated as median lethal concentrations (LC50) that were 13.28, 0.25, and 0.03 µg a.i. ml-1 for chlorantraniliprole, phosalone, and spinosad, respectively. The effect of a low lethal concentration (LC30) of the compounds was evaluated on various adult biological traits, such as longevity, fecundity, emergence rate and other life table parameters. All compounds caused detrimental effects on all the estimated demographical indexes. Chlorantraniliprole affected the net reproductive rate, mean generation time and doubling time in comparison to the control; while, phosalone and spinosad adversely affected all assessed parameters. Phosalone and spinosad significantly reduced gross reproductive rate, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time and doubling time and reduced longevity, fecundity, emergence rate related to other biological parameters in comparison with control. The results suggest that all compounds are not fully compatible with the activity of T. brassicae, and that the inclusion of chlorantraniprole, spinosad and phosalone into Integrated Pest Management (IPM) involving this parasitoid has to be avoided. Nevertheless, further studies in open field conditions and on a multiple generation scale are necessary for providing a more definitive conclusion on the IPM suitability of the three tested insectcides.

Behavioral and Biological Effects of Exposure to Tuta absoluta (Lepidoptera: Gelechiidae) Sex Pheromone on Several Trichogramma (Hymenoptera: Trichogrammatidae) Populations.

Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most destructive pests of tomato worldwide. Biological control of the pest using Trichogramma (Hymenoptera: Trichogrammatidae) wasps can be combined with other practices such as use of synthetic sex pheromones for mating disruption or mass trapping programs. In this study, effects of T. absoluta sex pheromone on behavioral responses and fertility life table parameters of Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae), Wolbachia-uninfected (W-) Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae), and Wolbachia-infected (W+) T. brassicae were investigated under laboratory conditions. Female wasps of T. evanescens and T. brassicae (W+) were attracted to the pest synthetic sex pheromone (around 62%) and calling virgin female moths (around 75%) and their responses were affected by the wasp age and temperature. Exposure to the host synthetic sex pheromone significantly reduced the percentage of adult emergence, longevity, and fecundity of female wasps, as well as the time spent to find a mate and duration of mating. However, tested populations were not affected similarly. Despite the laboratory tests, greenhouse experiments showed that the wasps were not caught in the traps baited with T. absoluta synthetic sex pheromone. Complementary studies are needed to precisely determine possible interference between pheromone application and releasing Trichogramma Westwood (Hymenoptera: Trichogrammatidae) wasps to achieve a successful integrated control of T. absoluta.