Cytotoxicity, Antimicrobial, Anti-inflammatory and Antioxidant Activity of Camellia Sinensis and Citrus Mediated Copper Oxide Nanoparticle-An In vitro Study.

Aim: Several applications of copper oxide nanoparticles (CuONPs) have been documented in various fields, including healthcare, dentistry, medication delivery, tissue and cancer imaging, biolabeling, and biosensing. Therefore, this study aimed to synthesize CuONPs using the plant extracts of Camellia Sinesis (CS) and citrus limon (CL). The nanoparticles were then evaluated for their cytotoxicity, antibacterial, anti-inflammatory, and antioxidant activities. Materials and Methods: CuONPs were prepared using CS and CL through the green synthesis method. The Zone of Inhibition (ZOI) test was used to assess the antibacterial activity against strains of Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, and Candida albicans. The albumin denaturation assay was used to assess the substances' anti-inflammatory activity. The cytotoxicity was determined by conducting the brine shrimp lethality test. Additionally, the antioxidant nature was tested using the 1,1-diphenyl-2-picryl hydrazyl method. Results: CuONPs mediated by CS and CL were successfully synthesized. The nanoparticles demonstrated significant antimicrobial activity against the bacteria being studied, specifically S. aureus. The cytotoxic effect was observed to be the least when the concentrations were below 20 µL. A potent antioxidant effect, characterized by its maximum absorbance at 517 nm, was observed at a concentration of 50 µL. A significant anti-inflammatory effect was noted for all tested concentrations. Conclusion: The use of CS- and CL-mediated CuONPs demonstrates a favorable antimicrobial effect with reduced cytotoxicity, as well as improved anti-inflammatory and antioxidant effects at higher concentrations.

ATM technique - a novel radiographic technique to assess the position of buccal shelf implants.

OBJECTIVES: Success of the buccal shelf implants depends on its proper placement. Radiographic techniques like cone beam computed tomography (CBCT), posteroanterior cephalogram (PA cephalogram) have been used to evaluate the position of buccal shelf implant placement. However, these techniques have disadvantages like increased radiation exposure, metal artifact, less availability in dental offices and are more expensive.The aim of Arvind's Trans Mandibular (ATM) technique is to overcome this drawback by using an intraoral periapical radiograph (IOPA) film or radiovisiography (RVG) sensor to assess the post-operative placement of buccal shelf implants. METHODS: Radiographs were taken for four patients after the placement of buccal shelf implants. The IOPA sensor was placed beneath the lower border of the mandible using this modified extraoral technique to assess the position of the buccal shelf implants in relation to the adjacent molars. The findings from the IOPA image were verified with CBCT images. RESULTS: The radiographic images taken using ATM technique were compared with the gold-standard CBCT technique. The ATM technique was able to accurately assess the postoperative buccolingual position of the implant with respect to the roots of molars in all the four cases. The proximity of the implant to the roots of the molar can be clearly visualized in all the cases. CONCLUSIONS: ATM is a novel radiographic technique using IOPA radiographic film or RVG sensor to assess the post-operative buccolingual placement of buccal shelf implants in relation to lower molar roots.

Association of severity of malocclusion with socioeconomic status in the South Indian population.

Malocclusion has been linked to various factors out of which certain dietary patterns and unhealthy habits are the most overlooked. The dietary patterns and unhealthy habits vary according to socioeconomic status. The present research was aimed to perform an association of malocclusion severity with socioeconomic status. This study was done in a retrospective manner and was conducted at Saveetha Dental College. A total of 241 clinical case records of the participants with malocclusion reporting for orthodontic therapy were selected and enrolled for the study. Data on the socioeconomic status and the severity of malocclusion as assessed with the Index of Orthodontic Treatment Needs (IOTNs) index were noted. All these records were collected and entered into Excel and then analyzed through statistics. Descriptive statistics and nonparametric Chi-square tests were performed. From the analysis, the proportion of IOTN Grade 1 malocclusion (30%) was found to be the highest. The highest number of patients with Grade 1 malocclusion belonged to the lower socioeconomic class. Socioeconomic status and the severity of malocclusion were significantly associated with each other. Malocclusion prevalence and severity were more among participants belonging to lower socioeconomic groups.

Association of vertical growth pattern with canine impactions in Dravidian subjects.

Canine impaction, ectopic canines, canine transposition, canine transmigration, and agenesis are all caused by disturbances during development and eruption of the teeth. The position of the canines is of utmost importance, and they should be carefully inspected as they follow the longest path during eruption in the oral cavity and their shape and position play a major role in occlusal guidance. The prevalence of canine impaction may also be connected to the pattern of facial growth. The objective of this study was to analyze the association of vertical growth pattern and canine impaction in Dravidian population. The dental records were consulted for information. The patient information was gathered from the outpatient data records of patients who were treated with fixed orthodontic treatment at the private dental setup. Patients with canine impactions were shortlisted. Malocclusion, growth pattern, and type of impaction were recorded. The data obtained were tabulated and analyzed using statistical software. Out of 1385 subjects, 35 patients had impacted canines. Among 35 patients with impacted canines, 23 (65.71%) have a vertical growth pattern, 6 (17.14%) have an average growth pattern, and 6 (17.14%) have a horizontal growth pattern. According to the findings of this investigation, there was no gender-related difference in canine impaction. The canine impaction prevalence in Dravidian subjects was found to be 2.53%. Most of them with impacted canines had a vertical growth pattern.

Severity of mandibular arch crowding in different sagittal malocclusions.

Mandibular anterior crowding is caused by a variety of factors. Mandibular incisor crowding can be caused by a number of causes including incisor and molar inclination, early loss of deciduous molars, mandibular growth, and oral musculature. The study was aimed to perform an association of the lower anterior crowding severity with gender and type of malocclusion. The current study was performed in a hospital setup and data about mandibular arch crowding patients were collected from the Records management system of a Private Dental Hospital in Chennai city. All the patient data on Mandibular arch crowding were sourced and tabulated after which statistical analysis with SPSS-IBM was done. Data collection was done over a period from June 2019 to February 2021. The entire study sample size was 634 case records. The result obtained from the statistical analysis was found that nearly 46% of the patients were found to have Mandibular arch crowding with female predilection (50%). The most commonly associated age groups were children than adults (63.2%) associated with mild type of crowding (65.1%). The most commonly involved malocclusion was found to be Class 1 (88.4%) Mild imbrications of the lower arch were common and were seen mainly in subjects with Class I malocclusion. Female subjects presented with more prevalence of mandibular arch crowding when compared to male subjects. Children were more affected by crowding than adults.

Synthesis, characterization, and antimicrobial activity of silver nanoparticles derived from Mentha X piperita + Ocimum tenuiflorum: An in vitro study.

The objective of the study was to synthesize silver nanoparticles using Mentha X Piperita (Mint) + Ocimum tenuiflorum (tulsi) and to confirm its size and shape. 0.5 mg of tulsi and 0.5 mg mint were diluted in distilled water (100 ml). The dissolved formulation was heated for 15 min at 70°C and filtered. The filtrate was homogeneously combined with 0.9 mg of silver nitrate to prepare nanoparticles of silver (AgNPs). The characterization of the obtained nanoparticle was done using transmission electron microscopy. Using agar disc diffusion assay, the antibacterial property was evaluated against common oral microbes at different concentrations. Silver nanoparticles showed excellent antimicrobial activity against Streptococcus mutans at 100 µL concentration. At 25 and 50 µL, all microbes showed similar extent of antimicrobial activity when quantified. Tulsi and mint prove to be effective in synthesizing silver nanoparticles that have good antimicrobial activity against oral microbes.

Association of crossbite with vertical skeletal growth patterns: A retrospective study.

Crossbite is a type of malocclusion in which teeth are positioned more buccally or lingually than their corresponding opposing tooth in the upper or lower dental arch, resulting in a lateral mismatch of the teeth in the dental arches. Crossbite can be unilateral or bilateral, and it can be anterior or posterior. The link between crossbite and skeletal growth pattern remains uncertain. The current study aimed to find the prevalence of crossbite in subjects visiting a private dental hospital and evaluate if there is any association between crossbite and skeletal growth pattern. Data required for the study were procured from the dental information archiving software. The data were sorted and entered into an Excel spreadsheet. IBM SPSS software version 23 was used to conduct the statistical analysis. Graphs and tables were used to interpret the findings. the prevalence of crossbite was found to be 6.4%. Subjects with horizontal growth pattern commonly had crossbite, but no statistical significance (P = 0.07), standard deviation (1.011), was noted. There was no statistically significant association between different skeletal growth patterns however subjects with horizontal growth pattern reported more commonly with crossbite.

Assessment of anterior open bite prevalence in children visiting a dental hospital - A retrospective evaluation.

Anterior open bite (AOB) is the insufficient vertical overlapping between the maxillary and mandibular anteriors when the teeth are in centric occlusion. The purpose of this study was to determine the prevalence of AOB in children and adolescents who visited a private dental hospital. In this study, subjects aged between 16 and 18 years with AOB were included. Data about orthodontic examination of patients were taken from preentered dental records of the hospital. Data of patients with AOB regarding age, gender, and intraoral and extraoral features were collected from the case records of the subjects, and statistical analysis was performed (Chi-square test and nonparametric correlations). The prevalence percentage of open bite in the anteriors was 2.7%, with more prevalence in Class 1 subjects (81.5%). Subjects with AOB had competent lips (81.8%), straight nasolabial angle (86.8%), and shallow palatal vault (49.5%). AOB had no significant relationship with the type of malocclusion, palatal vault, and lip competency, according to the Chi-square test. AOB and the nasolabial angle had a significant association. Both acute and right-angled nasolabial angles were commonly seen in adolescent patients with AOB. In children and adolescents visiting a private dental hospital in Chennai, the prevalence of AOB was found to be 2.7%. A significant association was observed between AOB and nasolabial angle.