RESUMEN
Di(2-ethylhexyl) phthalate (DEHP) is one of the most abundant plasticizers in common household products. It leaches from materials, resulting in exposure associated with detrimental health effects. The main objective of this study was to investigate how DEHP and its metabolite, mono(2-ethylhexyl) phthalate (MEHP), interact with and permeate lipid structures, namely vesicles and planar bilayers. Using dynamic light scattering, we observed significant changes in the size and polydispersity of L-α-phosphatidylcholine (egg PC) vesicles when incubated with DEHP but not MEHP at the same concentrations (100 and 200⯵M). We demonstrated that these effects are mitigated by pre-treatment with chitosan nanoparticles which adsorb the phthalates. Using quartz crystal microbalance with dissipation monitoring (QCM-D), we observed a concentration dependence on the interaction of DEHP with egg PC supported lipid bilayers (SLBs). QCM-D results suggested lipid removal for 5 and 100⯵M DEHP, and adsorption and potential embedment in the bilayer at 50 and 200⯵M DEHP. SLB mass decrease was observed for all concentrations of MEHP (5, 50, 100, and 200⯵M), suggesting lipid removal. We also investigated the permeability of DEHP and MEHP as well as several small molecules across a 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) suspended lipid bilayer. We found that DEHP and MEHP both had low permeabilities, but only DEHP remained associated with the bilayer. Exposure to DEHP and MEHP influenced how several common small molecules interacted with DOPC bilayers. Ultimately, this work provides insight into mechanisms of phthalate interactions with lipid structures, having implications for human health.